Evaluation of FAIMS Technology for Mass Spec Analysis of Chemical Cross-linked Peptides
Posters | 2019 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
IndustriesProteomics
ManufacturerThermo Fisher Scientific
Key wordsfaims, scx, peptides, crosslinked, dss, cross, fractionation, linked, mass, nce, max, unmodified, pierce, settings, hcd, lysine, identifications, enrichment, csms, dsbu, cid, xlinkx, improved, modifications, isolation, target, dsso, value, swissprot, search, width, bca, peptide, resolution, top, uniprot, sequest, terminus, used, lumos, cvs, combination, speed, injection, hela, complex, bsa, linking, intellectual, tribrid
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Optimization of FAIMS-XL-MS Workflow for Phospho-Enrichable Crosslinkers
2022|Thermo Fisher Scientific|Posters
Optimization of FAIMS-XL-MS Workflow for Phospho-Enrichable Crosslinkers Yuqi Shi1, Brand D. Groppe2, Leigh Foster3, Ryan Bomgarden3, Rosa Viner1, Thermo Fisher Scientific, 1San Jose, CA, 2Kansas City, MO, 3Rockford, IL Previously we described optimized FAIMS XL-MS workflow for instruments with ion-funnel…
Key words
faims, faimsphox, phoxdspp, dsppdsso, dssomagnetic, magneticagarose, agaroseidentified, identifiednta, ntabeads, beadsphospho, phosphoenrich, enrichenrichable, enrichablecrosslinkers, crosslinkerscrosslinker, crosslinkercrosslinked
IMSC: Optimization of crosslinked peptide analysis on an Orbitrap Fusion Lumos mass spectrometer
2016|Thermo Fisher Scientific|Posters
Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
Key words
crosslinked, crosslinkedbuurbu, buurbucleavable, cleavablecrosslinkers, crosslinkersethcd, ethcddsso, dssoprotein, proteinpeptides, peptidescrosslinking, crosslinkingidentified, identifiedxlinkx, xlinkxsequestht, sequesthtcid, cidlumos, lumosbsa
Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer
2016|Thermo Fisher Scientific|Posters
evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…
Key words
buurbu, buurbucrosslinked, crosslinkedcleavable, cleavablecrosslinkers, crosslinkersdsso, dssoethcd, ethcdsequestht, sequesthtlumos, lumoscrosslinking, crosslinkingfusion, fusiondss, dssxlinkx, xlinkxcid, cidorbitrap, orbitrappeptide
An optimized enrichment strategy for improved mass spec analysis of chemically crosslinked peptides
2018|Thermo Fisher Scientific|Posters
An optimized enrichment strategy for improved mass spec analysis of chemically crosslinked peptides Leigh Foster1, Ryan Bomgarden1, Erum Raja1, Chris Etienne1, Rosa Viner2, John Rogers1 1Thermo Fisher Scientific, Rockford, IL; 2Thermo Fisher Scientific, San Jose, CA Heat Protein Recovery (Background…
Key words
aadsbso, aadsbsocrosslinked, crosslinkedbiotinylation, biotinylationultralink, ultralinkstreptavidin, streptavidindsso, dssoneutravidin, neutravidincrosslinks, crosslinksbsa, bsadsbso, dsbsopeptides, peptideshela, helaagarose, agaroseenrichment, enrichmentavidin