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IMSC: Optimization of crosslinked peptide analysis on an Orbitrap Fusion Lumos mass spectrometer

 

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evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…
Key words
buurbu, buurbucrosslinked, crosslinkedcleavable, cleavablecrosslinkers, crosslinkersdsso, dssoethcd, ethcdsequestht, sequesthtlumos, lumoscrosslinking, crosslinkingfusion, fusiondss, dsscid, cidpeptide, peptidence, nceorbitrap
Evaluation of FAIMS Technology for Mass Spec Analysis of Chemical Cross-linked Peptides Rosa Viner1; Leigh A Foster2; Ryan D. Bomgarden2; Michael W. Belford1; Satendra Prasad1; Romain Huguet1; Eloy R. Wouters1 , 1Thermo Fisher Scientific, San Jose, CA; 2Rockford, IL ABSTRACT…
Key words
faims, faimsscx, scxpeptides, peptidescrosslinked, crosslinkeddss, dsscross, crossfractionation, fractionationlinked, linkedmass, massnce, ncemax, maxunmodified, unmodifiedpierce, piercesettings, settingshcd
Crosslinking mass spectrometry (XL-MS) goes mainstream
2018|Thermo Fisher Scientific|Technical notes
proteomics WHITE PAPER 65343 Crosslinking mass spectrometry (XL-MS) goes mainstream Mass spectrometrists and structural biologists guide to using XL-MS to understand the structure and function of molecular machines Author Executive summary Julian Saba, Thermo Fisher Scientific, San Jose, CA, USA…
Key words
crosslinked, crosslinkedprotein, proteinpeptides, peptidescrosslinking, crosslinkingcrosslinkers, crosslinkersdsso, dssointeractions, interactionsmass, masspeptide, peptidecan, canions, ionsproteins, proteinscleavable, cleavablecomplexes, complexesresearchers
An optimized enrichment strategy for improved mass spec analysis of chemically crosslinked peptides Leigh Foster1, Ryan Bomgarden1, Erum Raja1, Chris Etienne1, Rosa Viner2, John Rogers1 1Thermo Fisher Scientific, Rockford, IL; 2Thermo Fisher Scientific, San Jose, CA Heat Protein Recovery (Background…
Key words
aadsbso, aadsbsocrosslinked, crosslinkedbiotinylation, biotinylationultralink, ultralinkstreptavidin, streptavidindsso, dssoneutravidin, neutravidincrosslinks, crosslinksbsa, bsadsbso, dsbsopeptides, peptideshela, helaenrichment, enrichmentagarose, agaroseavidin
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