Waters PURIFICATION Product Guide

The Importance of the Topic

Effective sample purification underpins high-quality analytical results by removing interferences and isolating target compounds in research, quality control, and process development. Advanced liquid chromatography (LC) and supercritical fluid chromatography (SFC) workflows increase throughput, reliability, and sustainability.

Objectives and Portfolio Overview

This guide reviews Waters’ purification solutions spanning semi-automated modular LC systems to fully automated mass-directed platforms and environmentally friendly SFC systems. It aims to help laboratories select systems for scales from micrograms to kilograms.

Methodology and Used Instrumentation

Waters’ purification approaches leverage gradient-based HPLC and SFC separations with UV and mass spectrometric detection. Key components include:

• Fluid delivery modules: 1525 EF pump, 2535 and 2545 Quaternary Gradient Modules, 2545 Binary Gradient Module, 2555 Quaternary Gradient Module
• Sample handling: 2707 Autosampler, 3767 Sample Manager, Waters Fraction Collector III, Waters Fraction Manager – Analytical
• Detectors: 2489 UV/Vis, 2998 PDA, SQ Detector 2, ACQUITY QDa, 2424 ELS
• Software: MassLynx with FractionLynx Application Manager, Empower, ChromScope

Scalable preparative columns include OBD-based chemistries and Torus SFC phases.

Main Features and Discussion

LC systems range from the basic LC Prep 150 for low-medium throughput to the AutoPurification series offering dual flow paths, high throughput, and mass-directed fraction collection. Modular configurations support flexible solvent delivery and detection options. SFC platforms such as the SFC Prep 150 AP, MGM, 350, Investigator, and 15 systems deliver high-speed chiral and achiral separations with green CO₂-based mobile phases.

Benefits and Practical Applications

• Scalable performance from analytical screening to preparative isolation
• High specificity through UV and MS-directed fraction collection
• Enhanced productivity via automated sample handling and software-driven workflows
• Reduced solvent consumption and environmental impact with SFC
• Robust hardware supporting complex mixtures and chiral compound purification

Future Trends and Application Possibilities

Integration of real-time data analytics and AI-based method optimization will further streamline purification workflows. Advances in sustainable mobile phase recycling, miniaturized flow cells, and enhanced mass detectors promise higher efficiency and lower costs. The convergence of chromatography with continuous flow and process analytical technology will expand preparative capabilities in pharmaceutical and industrial settings.

Conclusion

Waters’ purification portfolio offers flexible, high-performance solutions tailored to diverse purification needs. By combining modular hardware, sensitive detection, and intuitive software, laboratories can achieve reliable compound isolation, accelerated workflows, and sustainable operations.

References

No references were provided in the original document.