Cary 4000/5000/6000i UV-Vis spectrophotometer – Site Preparation Checklist

Significance of Site Preparation

Ensuring correct laboratory setup is critical for reliable performance and longevity of UV-Vis spectrophotometers. Proper bench space, environmental control, and utility services prevent instrument damage, maintain measurement accuracy, and support efficient workflows.

Objectives and Overview

This checklist outlines the prerequisites for installing Agilent Cary 4000/5000/6000i spectrophotometers. It guides users through space allocation, environmental requirements, power setup, and necessary supplies to facilitate a smooth installation.

Methodology and Instrumentation

The document employs a structured checklist approach covering:

• Laboratory bench dimensions and weight capacity
• Ambient conditions (temperature, humidity, vibration, contamination control)
• Electrical power specifications (voltage, grounding, outlet placement)
• Optional nitrogen purging setup for enhanced optical performance

The primary instrument is the Agilent Cary UV-Vis spectrophotometer models 4000, 5000, and 6000i.

Main Requirements and Discussion

Bench Space and Weight Capacity

• Height: ~90 cm, stable support for up to 141 kg (packed)
• Clearance: ≥2 in at sides, ≥6 in rear, ≥8 in front for air circulation and accessory access

Environmental Conditions

• Temperature: 20–25 °C ±2 °C; non-operating: 5–45 °C
• Humidity: ≤90% non-condensing; operating within specs: 15–80%
• Contaminant-free atmosphere; separate sample prep area to avoid vapors and particulates

Power and Utilities

• 100–240 VAC ±10%, 50–60 Hz; single-phase three-wire system
• Dedicated grounded outlet; avoid extension cords and interference sources
• Power consumption: max 300 VA (185 W)

Optional Supplies

• Quartz cuvettes (pair)
• Nitrogen purging system: dry, oil-free gas at 12–25 psi; tubing and manifold with flow meters

Benefits and Practical Applications

Adherence to these guidelines ensures:

• Optimal instrument stability and measurement precision
• Reduced downtime and maintenance needs
• Safe operation and compliance with warranty conditions

Future Trends and Potential Applications

Integration of spectrophotometers with automated sample handling and IoT-enabled environmental monitoring can further enhance reliability. Advances in software-driven diagnostics and remote support will streamline installation and maintenance. Sustainable laboratory practices, such as energy-efficient components and closed-loop nitrogen recycling, are expected to gain traction.

Conclusion

Thorough site preparation is a foundational step for successful UV-Vis spectrophotometer operation. Following the outlined checklist minimizes installation risks, preserves instrument integrity, and supports accurate analytical outcomes.

Použitá instrumentace

Agilent Cary 4000 UV-Vis spectrophotometer

Agilent Cary 5000 UV-Vis spectrophotometer

Agilent Cary 6000i UV-Vis spectrophotometer

Reference

Agilent Technologies. Cary 4000/5000/6000i UV-Vis Spectrophotometer – Site Preparation Checklist. Publication G9825-90000, Revision 2.0, November 2016.