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IMSC: Development of an All-Recombinant Intact Protein Standard for LC-MS Application Development and System Suitability Testing

Posters | 2016 | Thermo Fisher ScientificInstrumentation
Consumables, LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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Improving high-throughput Top-Down proteomics using a modified hybrid quadrupole-ultra-high-field-Orbitrap mass spectrometer Eugen Damoc1, Kyle L. Fort2, Michiel van de Waterbeemd2, Sem Tamara2, Christian Thoeing1, Erik Cousijn1, Tabiwang Arrey1, Alexander Harder1, Albert J. R. Heck2 and Alexander Makarov1 Fisher Scientific (Bremen)…
Key words
ftms, ftmsabundance, abundancedda, ddatop, topdown, downrelative, relativesliding, slidingdeconvolution, deconvolutionhigh, highmasses, massesesi, esifull, fullwindow, windowxtract, xtractproteins
Evaluation of Novel FAIMS Technology for Intact Protein Detection and Characterization by Infusion Susan E. Abbatiello1, Jason Neil1, William McGee1, Scott Kronewitter1, Michael Belford2, Jim Stephenson1, Mary Blackburn2, 1Thermo Fisher Scientific, Cambridge, MA, 2Thermo Fisher Scientific, San Jose, CA ABSTRACT…
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faims, faimsvoltages, voltagescompensation, compensationoff, offinfusion, infusionprotein, proteinproteoforms, proteoformsspe, spevoltage, voltageproteoform, proteoformdesalted, desaltedafter, afterlumos, lumoslysate, lysatestep
Methods Sample Preparation and Liquid Chromatography Protein standards (Ubiquitin, Carbonic anhydrase and Enolase) were purchased from Sigma Aldrich. Proteins were infused at 1pmol/ul at a flow rate 5ul/min for direct infusion experiments. Recombinant Human TROP2 protein (extracellular domain) was purchased…
Key words
etd, etdlumos, lumosorbitrap, orbitraptop, topdown, downfusion, fusionintact, intactproteins, proteinsfragmentation, fragmentationenolase, enolasecoverage, coveragesequence, sequenceprotein, proteinwere, werecarbonic
peak coalescence and resolution. W le (O lim m slo m Methods: A Thermo ScientificTM OrbitrapTM FusionTM TribridTM mass spectrometer was modified by (1) improvements to the vacuum system aimed at reducing the amount of gas in the ion injection…
Key words
thethe, thetheforfor, forforatat, atatirm, irmcoalescence, coalescencepressures, pressuresanhydrase, anhydrasefigure, figurecarbonic, carbonicfusion, fusionimprovements, improvementsorbitrap, orbitrapdecay, decayimprovement, improvementonset
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