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Improving high-throughput Top-Down proteomics using a modified hybrid quadrupole-ultra-high-field-Orbitrap mass spectrometer

Posters | 2017 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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Poster Note 64778 Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov Thermo Fisher Scientific, San…
Key words
abundance, abundancefragmentation, fragmentationantibody, antibodyintact, intactetd, etdrelative, relativemab, mabtop, topdown, downheavy, heavyuvpd, uvpdmonoclonal, monoclonaldeglycosylated, deglycosylatedpulses, pulsessequence
A Complete Workflow Solution for Intact Monoclonal Antibody Characterization Using a New High-Performance Benchtop Quadrupole-Orbitrap LC-MS/MS Zhiqi Hao,1 Yi Zhang,1 David Horn,1 Seema Sharma,1 Shiaw-Lin Wu, 2 Irene Ae-Ning Lin, 3 Yi-Hsuan Pan, 3 Ya-Fen Yang, 3 and Andreas F.…
Key words
mab, mababundance, abundanceintact, intactrelative, relativeexactive, exactivehcd, hcdspectrum, spectrumfile, fileorbitrap, orbitrapenvelope, envelopemass, masscharge, chargedeconvolution, deconvolutiondown, downantibody
For bottom-up experiments, the monoclonal antibodyy Rituximab was denatured for 30 min in 7 M Urea and 50 mM Tris HCL at pH 8.00. The sample was reduced with 5 mM 37°C and alkylated by addition of 10 mM IAA…
Key words
top, topdown, downbottom, bottomfigure, figuresequest, sequestbyonic, byonicndance, ndanceabun, abunterminal, terminalrelative, relativeprosight, prosighttthe, tthezoominto, zoomintokfl, kfldiscoverer
APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…
Key words
intact, intacttrastuzumab, trastuzumabnative, nativemass, masshcd, hcdmab, mabthermo, thermosubunit, subunitdown, downdeconvolution, deconvolutionscientific, scientificsubunits, subunitspeptide, peptidemiddle, middleheavy
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