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A Complete Workflow Solution for Intact Monoclonal Antibody Characterization Using a New High-Performance Benchtop Quadrupole-Orbitrap LC-MS/MS

Posters | 2012 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Clinical Research
Manufacturer
Thermo Fisher Scientific
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Poster Note 64778 Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov Thermo Fisher Scientific, San…
Key words
abundance, abundancefragmentation, fragmentationantibody, antibodyintact, intactetd, etdrelative, relativemab, mabtop, topdown, downheavy, heavyuvpd, uvpdmonoclonal, monoclonaldeglycosylated, deglycosylatedpulses, pulsessequence
Martin Samonig1,2, Christian Huber1,2 and Kai Scheffler2,3 1 Division of Chemistry and Bioanalytics, University of Salzburg, Salzburg, Austria 2 Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Salzburg, Austria 3 Thermo Fisher Scientific, Dreieich, Germany Key…
Key words
abundance, abundanceeeqynstyr, eeqynstyrintact, intactrelative, relativechain, chainantibody, antibodymonolithic, monolithiclight, lightmass, massmonoclonal, monoclonalheavy, heavyaif, aiffragmentation, fragmentationmonoisotopic, monoisotopictrypsin
experiments were performed yielding 100% sequence coverage, site specific information of the drug-conjugate as well as expected and unexpected modifications. 10 21 0 Methods Sample Preparation M N LC Poster Note 643 97 The T-DM1 sample was reduced by DTT…
Key words
intact, intactabundance, abundancepeptide, peptidefigure, figurerelative, relativemolecule, moleculelight, lightadcs, adcsmass, massweight, weightmapping, mappingpepfinder, pepfinderheavy, heavyglycoforms, glycoformsthermo
APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…
Key words
intact, intacttrastuzumab, trastuzumabnative, nativemass, masshcd, hcdmab, mabsubunit, subunitdown, downdeconvolution, deconvolutionthermo, thermoscientific, scientificsubunits, subunitspeptide, peptidemiddle, middleheavy
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