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HILIC Chromatography: When and How?

 

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Application Note Small Molecule Pharma Retaining and Separating Polar Molecules—A Detailed Investigation of When to Use HILIC Versus a Reversed-Phase LC Column Author Anne Mack Agilent Technologies, Inc. Abstract This work examines when to use hydrophilic interaction LC (HILIC) and…
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Thinking Outside the C18 Box 1 Modes of HPLC • Reversed-phase • C18, C8, other non-polar phases • Phenyl, PFP, other more polar phases • Ion Pairing • HILIC • Silica • Amide • Zwitterionic • Hydroxyl-based • Chiral •…
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