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MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography

 

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Application Note Pharma & Biopharma Oligonucleotides Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF Oligonucleotide identity, impurities analysis and sequence determination using BioConfirm 12.0 target plus impurities (TPI) and sequence confirmation workflows Authors Introduction Bian…
Key words
oligonucleotide, oligonucleotideaso, asosequence, sequencetpi, tpicounts, countsacquisition, acquisitionconfirmation, confirmationoligonucleotides, oligonucleotidesworkflow, workflowcharge, chargeimpurities, impuritiesbioconfirm, bioconfirmmass, massagilent, agilentfluoro
Application Note High-throughput, Ion-Pairing-Free, HILIC Analysis of Oligonucleotides Using Agilent RapidFire Coupled to Quadrupole Time-of-Flight Mass Spectrometry Author Abstract Peter Rye, PhD Agilent Technologies, Inc. This application note describes a high-throughput, ion-pairing-free method for oligonucleotide characterization using the Agilent RapidFire…
Key words
oligo, oligorapidfire, rapidfirecounts, countshilic, hilicoligos, oligosdeconvoluted, deconvolutedcharge, chargeamu, amumass, massiprp, iprpdepurination, depurinationpredominant, predominantwere, weredeconvolution, deconvolutionheights
Application Note BioPharma MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography Authors Introduction Peter Rye and Cody Schwarzer Agilent Technologies, Inc. The synthesis of RNA and DNA oligonucleotides (oligos) is an iterative process that, despite highly optimized chemistry, results in…
Key words
counts, countsdeconvoluted, deconvolutedsirna, sirnaoligos, oligosamu, amumass, masscharge, chargeoligo, oligohilic, hilicribose, riboseimpurities, impuritiesduplex, duplexwide, widedepur, depurtime
Application Note Biopharma/Pharma Comprehensive and Integrated Workflow for Oligonucleotide Sequence Confirmation by Agilent High-Resolution LC/Q-TOF Robust and sensitive sequence confirmation of synthetic oligonucleotides and impurities Authors David L. Wong and Peter Rye Agilent Technologies, Inc. Abstract This application note demonstrates…
Key words
oligonucleotide, oligonucleotidesequence, sequenceoligonucleotides, oligonucleotidesconfirmation, confirmationcounts, countscharge, chargemass, massfragment, fragmentworkflow, workflowrna, rnaimpurities, impuritiessequences, sequencesladder, ladderdata, dataacquisition
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