Agilent InfinityLab High Throughput 384-Well Plate, 0.19 mL, Square Wells, V Shape, Polypropylene, 22 mm

Importance of the topic

High-density sample storage and recovery are critical in modern analytical workflows, especially for high-throughput chromatography and automated screening. Efficient handling of small volumes ensures reproducibility, reduces waste, and accelerates sample throughput in drug discovery, proteomics, environmental testing, and other applications.

Study objectives and overview

This document presents the design and performance characteristics of the Agilent InfinityLab High Throughput 384-Well Plate (0.19 mL, square V-shaped wells). The goal is to provide an overview of its physical dimensions, material properties, and compatibility with laboratory sealing and automation systems.

Methodology and product specifications

The plate was manufactured from medical-grade virgin polypropylene, chosen for its low biomolecular binding and broad chemical compatibility. Key features include:

• 384 square wells, each 3.5 mm per side, 19 mm deep, with a total nominal volume of 190 µL and working volume of 170 µL
• V-shaped well bottoms to maximize small-volume recovery by autosamplers
• Clear polypropylene construction for uniform sealing and optical compatibility
• Alphanumeric grid for sample tracking
• SBS-compliant footprint (127.8 × 85.5 mm) and stackable design
• Temperature tolerance from –80 °C to 120 °C; autoclavable and chemically resistant (including DMSO, phenol, chloroform)

Main findings and discussion

Performance testing and specification review demonstrate:

• Excellent sample recovery efficiency due to V-shaped wells, minimizing dead volume in automated sampling
• Structural integrity across a wide temperature range, enabling cryogenic storage and high-temperature processing
• Low adsorption of biomolecules and nucleic acids, reducing sample loss and carryover
• Reliable sealing compatibility reduces evaporation and contamination risks during storage or reaction incubation

Benefits and practical applications

The plate supports a variety of workflows:

• High-throughput screening in pharmaceutical and biotech research
• Sample storage for genomic, proteomic, and metabolomic assays
• Automated liquid handling for compound libraries
• Quality control in clinical and environmental laboratories

Future trends and potential applications

Advances in microplate design may include:

• Even higher well densities for ultra-high-throughput assays
• Integrations with barcoding and RFID for improved sample tracking
• Surface modifications to further minimize nonspecific binding
• Compatibility with next-generation automated platforms employing robotics and AI-driven scheduling

Conclusion

The Agilent InfinityLab 384-Well Plate offers a robust solution for high-throughput sample handling. Its optimized geometry, chemical resistance, and temperature stability make it a versatile consumable for a wide range of analytical and preparative applications.

Reference

Agilent Technologies. Data Sheet: InfinityLab High Throughput 384-Well Plate, 0.19 mL, Square V Shape, Polypropylene, 22 mm, Part Number 5043-9315. 2023.