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Characterization of adeno-associated viral proteins proteoforms using a top-down MS approach on a UHPLC-Orbitrap Ascend Tribrid mass spectrometer

Applications | 2023 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Pharma & Biopharma, Proteomics
Manufacturer
Thermo Fisher Scientific
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APPLICATION NOTE 000253 Highly sensitive intact mass analysis of AAV capsid proteins using a UHPLC-FLDHRAM MS platform Authors: Reiko Kiyonami¹, Kristina Srzentić², Rosa Viner¹, Weijing Liu¹, Min Du², Katy Irvin³, Kenneth Thompson³, Chao Yan Liu³, Jon Zmuda³ ¹ Thermo Fisher…
Key words
capsid, capsidaav, aavintact, intactviral, viraltop, topdown, downproteins, proteinsprotein, proteinmass, masssequence, sequenceintensity, intensityvanquish, vanquishrelative, relativefragment, fragmentthermo
Characterization of Adeno-Associated Viral assemblies on an Ultra-High Mass Range Hybrid QuadrupoleOrbitrap Mass Spectrometer Kristina Srzentić1, Eugen Damoc2, Marc Günder1 1Thermo Fisher Scientific, Reinach, Switzerland 2Thermo Fisher Scientific, Bremen, Germany ABSTRACT Purpose: Evaluate the performance of the Thermo Scientific™ Q…
Key words
viral, viralaav, aavintact, intactmass, massadeno, adenoanalysis, analysisassemblies, assembliesdenaturing, denaturingunder, underassociated, associatedstoichiometries, stoichiometriesvanquish, vanquishnative, nativeassembly, assemblynear
Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…
Key words
mass, massaav, aavtruncated, truncatedfld, fldviral, viralintact, intactintensity, intensitytheoretical, theoreticalrelative, relativeerror, errorppm, ppmsequence, sequenceobserved, observedprotein, proteinproteins
Multiple ion activations and proton transfer charge reduction facilitate the characterization of capsid proteoforms of recombinant adeno-associated virus 6 (rAAV6) Jake T. Kline1, Kristina Srzentić2, Katalyn Irvin3, Christopher Mullen4, Romain Huguet4, John EP Syka4, Luca Fornelli1 1University of Oklahoma, Norman,…
Key words
capsid, capsidproton, protonactivations, activationsicosahedral, icosahedralion, ionregion, regionmatched, matchedcharge, chargedistribution, distributionproteins, proteinsproteoforms, proteoformspolypeptide, polypeptideadeno, adenoaav, aavtruncation
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