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Highly sensitive intact mass analysis of AAV capsid proteins using a UHPLC-FLD- HRAM MS platform

Applications | 2021 | Thermo Fisher ScientificInstrumentation
HPLC, LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
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Pharma & Biopharma
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Thermo Fisher Scientific
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Application note | 002599 Biopharma Characterization of adeno-associated viral proteins proteoforms using a top-down MS approach on a UHPLC-Orbitrap Ascend Tribrid mass spectrometer Application benefits Authors Reiko Kiyonami , Rafael Melani , 1 1 • Confident determination of PTM localization…
Key words
fragment, fragmenttop, topdown, downascend, ascendviral, viralethcd, ethcduvpd, uvpdion, ionaav, aavtypes, typesdissociation, dissociationtribrid, tribridproteoforms, proteoformsintact, intactsequence
Characterization of Adeno-Associated Viral assemblies on an Ultra-High Mass Range Hybrid QuadrupoleOrbitrap Mass Spectrometer Kristina Srzentić1, Eugen Damoc2, Marc Günder1 1Thermo Fisher Scientific, Reinach, Switzerland 2Thermo Fisher Scientific, Bremen, Germany ABSTRACT Purpose: Evaluate the performance of the Thermo Scientific™ Q…
Key words
viral, viralaav, aavintact, intactmass, massadeno, adenoanalysis, analysisassemblies, assembliesdenaturing, denaturingunder, underassociated, associatedstoichiometries, stoichiometriesnative, nativeassembly, assemblyvanquish, vanquishnear
Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…
Key words
mass, massaav, aavtruncated, truncatedfld, fldviral, viralintact, intactintensity, intensitytheoretical, theoreticalrelative, relativeerror, errorppm, ppmsequence, sequenceobserved, observedprotein, proteinproteins
Assessing key attributes of adeno-associated viral proteins using UHPLC-FLD-intact accurate mass analysis Reiko Kiyonami1, Roberto Gamez1, Min Du2, Kenneth Thompson3, Chao Yan Liu3, Julia Kraegenbring4, Catharina Crone4 1Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Cambridge, MA, USA;…
Key words
viral, viralaav, aavproteins, proteinsptms, ptmsfld, fldintact, intactorbitrap, orbitrapmass, massexploris, explorisassociated, associatedvps, vpssummarized, summarizedenabled, enabledserotype, serotypeattributes
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