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Assessing key attributes of adeno-associated viral proteins using UHPLC-FLD-intact accurate mass analysis

Posters | 2022 | Thermo Fisher Scientific | ASMSInstrumentation
HPLC, LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…
Key words
mass, massaav, aavtruncated, truncatedfld, fldviral, viralintact, intactintensity, intensitytheoretical, theoreticalrelative, relativeerror, errorppm, ppmsequence, sequenceobserved, observedprotein, proteinproteins
APPLICATION NOTE 000253 Highly sensitive intact mass analysis of AAV capsid proteins using a UHPLC-FLDHRAM MS platform Authors: Reiko Kiyonami¹, Kristina Srzentić², Rosa Viner¹, Weijing Liu¹, Min Du², Katy Irvin³, Kenneth Thompson³, Chao Yan Liu³, Jon Zmuda³ ¹ Thermo Fisher…
Key words
capsid, capsidaav, aavintact, intactviral, viraltop, topdown, downproteins, proteinsprotein, proteinmass, masssequence, sequenceintensity, intensityvanquish, vanquishrelative, relativethermo, thermofragment
Characterization of Adeno-Associated Viral assemblies on an Ultra-High Mass Range Hybrid QuadrupoleOrbitrap Mass Spectrometer Kristina Srzentić1, Eugen Damoc2, Marc Günder1 1Thermo Fisher Scientific, Reinach, Switzerland 2Thermo Fisher Scientific, Bremen, Germany ABSTRACT Purpose: Evaluate the performance of the Thermo Scientific™ Q…
Key words
viral, viralaav, aavintact, intactmass, massadeno, adenoanalysis, analysisassemblies, assembliesdenaturing, denaturingunder, underassociated, associatedstoichiometries, stoichiometriesassembly, assemblynative, nativevanquish, vanquishnear
[ APPLICATION NOTE ] Optimizing Adeno-Associated Virus (AAV) Capsid Protein Analysis Using UPLC and UPLC-MS Ximo Zhang, Stephan Koza, Ying Qing Yu, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ INTRODUCTION An optimized LC-MS solution for…
Key words
capsid, capsidaav, aavadeno, adenouplc, uplcvirus, virusassociated, associatedproteins, proteinsoptimizing, optimizingraav, raavmass, massflr, flrtherapy, therapygene, geneacquity, acquityprotein
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