Instructions for use Mitra® 96-Autorack™ (IVD)

Importance of the topic

Accurate microsampling of blood is essential for reliable bioanalytical workflows, enabling consistent sample volumes with minimal invasiveness. The Mitra 96-Autorack system addresses the growing demand for high-throughput, standardized collection of microsamples in clinical and research laboratories.

Objectives and overview

This protocol provides step-by-step instructions for using the Mitra 96-Autorack rack and Mitra samplers to collect precise volumes of blood from tubes. Key goals include ensuring sample integrity, reproducible volumes, and efficient drying for downstream analysis.

Methodology and instrumentation used

• Label samplers and rack according to laboratory protocol.
• Lift 96-Autorack from collection plate and position next to it.
• Remove a sampler, touch the sampling tip to the blood surface until fully red, hold for 2 seconds, then remove tip slowly without submerging entirely.
• Return sampler to rack, avoiding contact with rack walls.
• Dry sampling tips under ambient conditions (minimum 3 hours) with the collection plate removed to allow free air circulation.
• Once dry, return samplers to the collection plate for storage or shipment.

Used instrumentation

• Mitra Microsampling Device (Mitra samplers and 96-Autorack rack)
• Collection plate for initial sample support

Main results and discussion

The standardized handling steps ensure uniform microsample volumes and minimize contamination risks. Critical factors such as tip orientation, avoidance of dripping, and adequate drying time contribute to sample quality and reproducibility. Ambient drying without the plate accelerates moisture removal while protecting sampler tips.

Benefits and practical application

This single-use, non-sterile device streamlines blood microsampling by providing fixed volumes in a 96-position format. It supports high-throughput workflows, reduces sample preparation variability, and enhances laboratory biosafety. The ambient drying process simplifies logistics by eliminating the need for specialized drying equipment.

Future trends and possibilities of use

Future developments may include integration with automated sampling platforms, remote or point-of-care collection kits, and adaptation for other biological matrices. Coupling the Mitra system with advanced bioanalytical techniques will expand its application in pharmacokinetics, therapeutic drug monitoring, and diagnostic screening.

Conclusion

The Mitra 96-Autorack protocol offers a robust, user-friendly approach for precise blood microsampling, ensuring reproducible sample collection and efficient drying. Its high-throughput design and streamlined workflow meet the evolving needs of modern analytical laboratories.