Instructions for use Mitra® 96-Autorack™ (RUO)

Importance of Microsampling

This protocol ensures consistent collection of small blood volumes for research applications. It supports high-throughput sampling while preserving sample integrity and enabling ambient drying and transport.

Goals and Overview

The guide describes a step-by-step workflow for collecting precise blood microsamples using the Mitra® 96-Autorack. It aims to standardize operations, reduce variability and facilitate downstream analytical procedures.

Methodology and Instrumentation

• Mitra® 96-Autorack device and compatible samplers
• Collection plate for temporary placement and transport
• Ambient drying setup allowing air circulation around sampling tips

The sampling procedure comprises six main steps:

1. Label the Autorack and individual samplers according to protocol requirements
2. Detach the Autorack from the collection plate and position it alongside
3. Touch each sampler tip to the blood surface until fully red, wait 2 seconds, then withdraw slowly while maintaining tip downward
4. Return each sampler to the Autorack without contacting the side walls
5. Dry the samplers under ambient conditions for at least three hours, ensuring free air flow by removing the collection plate
6. Transfer dried samplers back to the collection plate for storage or shipment

Main Findings and Discussion

Following this workflow yields uniform sample volumes and consistent drying outcomes. Proper tip orientation and avoidance of fluid submersion prevent sample loss or contamination. The ambient drying step removes the need for specialized equipment during shipment.

Benefits and Practical Applications

• High-throughput capacity of 96 samples per rack
• Minimal blood volume requirements for less invasive sampling
• Ambient drying and transport reduce cold-chain dependence
• Standardized handling improves reproducibility in analytical assays

Future Trends and Applications

Emerging developments may include integration with automated robotics for sampling and drying, remote or point-of-care collection kits for decentralized studies, and adaptation of the protocol to additional biofluids beyond blood.

Conclusion

The Mitra® 96-Autorack microsampling protocol provides a reliable, scalable and user-friendly approach to blood sample collection in research settings. Adherence to the defined steps ensures sample consistency, efficient throughput and compatibility with downstream analytical workflows.