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Evaluation of search engines for phosphopeptide identification and quantitation

 

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Xiaoyue Jiang1, Ryan Bomgarden2, Joseph Brown1, Rosa Viner1, Andreas R Huhmer1 1 Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Rockford, IL Poster Note 64779 Sensitive and Accurate Quantitation of Phosphopeptides Using TMT Isobaric Labeling Technique Sensitive and accurate…
Key words
phosphopeptides, phosphopeptidesphosphopeptide, phosphopeptidetrigger, triggerconfident, confidenthcd, hcdpeptides, peptidestmt, tmtneutral, neutrallocalizaton, localizatonunenriched, unenrichedloss, losssps, spsphosphoproteome, phosphoproteomemsa, msapeptide
Xiaoyue Jiang1, David Horn1, Michael Blank1, Devin Drew1, Bernard Delanghe2, Rosa Viner1, Andreas FR Huhmer1 1 Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Bremen, Germany Key Words Protein coverage, post-translational modification (PTM), Proteome Discoverer, SEQUEST, Byonic, Mascot,…
Key words
byonic, byonicsequest, sequestsearch, searchmaxquant, maxquantpropionyl, propionylmascot, mascotpeptide, peptidefdr, fdrprotein, proteinproteome, proteomeengines, enginesdiscoverer, discovererengine, engineptm, ptmhela
Thermo Scientific Proteome Discoverer software
2022|Thermo Fisher Scientific|Brochures and specifications
The intelligent protein informatics platform Thermo Scientific Proteome Discoverer software Transform proteomics mass spectrometry data into insights Thermo Scientific™ Proteome Discoverer™ software enables comprehensive proteomics data processing workflows empowered by artificial intelligence. • Powerful and flexible framework: Optimized analysis for…
Key words
inferys, inferysrescoring, rescoringdiscoverer, discovererproteome, proteomechimerys, chimeryspsms, psmsworkflows, workflowslfq, lfqpeptides, peptidessearch, searchtmt, tmtpeptide, peptidesequest, sequestproteomics, proteomicsprotein
Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
Key words
crosslinked, crosslinkedbuurbu, buurbucleavable, cleavablecrosslinkers, crosslinkersethcd, ethcddsso, dssoprotein, proteinpeptides, peptidescrosslinking, crosslinkingidentified, identifiedxlinkx, xlinkxsequestht, sequesthtcid, cidlumos, lumosbsa
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