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Optimizing Adeno-Associated Virus (AAV) Capsid Protein Analysis Using UPLC and UPLC-MS

 

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Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…
Key words
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Developing the analytics and analytical workflows supporting the analysis of the next generation of biotherapeutic and gene therapies. Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D. Waters Lunch Seminar Tuesday January 28, 2020 WCBP 2020 Diamond Program Partner ©2020 Waters…
Key words
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Comprehensive AAV Characterizations Using HRMS: Peptide Mapping, Capsid Proteins, Intact ssDNA, and Intact Virus T1430-09-53 Hua Yang, Ying Qing Yu, Stephan Koza, Suraj Dhungana, Ann Gray Waters Corporation, 34 Maple Street, Milford, Massachusetts, USA CONTACT INFORMATION: [email protected] PURPOSE RESULT(S) CONCLUSION(S)…
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APPLICATION NOTE 000253 Highly sensitive intact mass analysis of AAV capsid proteins using a UHPLC-FLDHRAM MS platform Authors: Reiko Kiyonami¹, Kristina Srzentić², Rosa Viner¹, Weijing Liu¹, Min Du², Katy Irvin³, Kenneth Thompson³, Chao Yan Liu³, Jon Zmuda³ ¹ Thermo Fisher…
Key words
capsid, capsidaav, aavintact, intactviral, viraltop, topdown, downproteins, proteinsprotein, proteinmass, masssequence, sequenceintensity, intensityvanquish, vanquishrelative, relativethermo, thermofragment
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