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Application NoteHigh Resolution Size-ExclusionChromatography Separations of mAbAggregates, Monomers, and Fragmentsusing BioResolve SEC mAb Columns onUPLC, UHPLC, and HPLC ChromatographySystemsPamela C. Iraneta, Stephan M. KozaWaters CorporationFor research use only. Not for use indiagnostic procedures. AbstractWaters BioResolve SEC mAb, 200 Å, 2.5 µm...
Key words
mab, mabdispersion, dispersionvariant, variantbioresolve, bioresolvesec, secsize, sizebio, bioacquity, acquitysystem, systemwaters, watersuplc, uplcresolution, resolutionseparations, separationsaggregates, aggregatescolumn
Impact of LC System Dispersion on the Size-Exclusion ChromatographyAnalysis of Monoclonal IgG Antibody Aggregates and Fragments:Selecting the Optimal Column for Your MethodStephan M. Koza, Corey E. Reed, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■An educational and systematicHistorically, native size-exclusion...
Key words
hmws, hmwsdispersion, dispersionaggregates, aggregatesigg, iggexclusion, exclusionantibody, antibodyacquity, acquitybio, biominutes, minutesimpact, impactsize, sizemonomer, monomersystem, systemmonoclonal, monoclonaluplc
High Resolution and High Throughput Size-Exclusion ChromatographySeparations of IgG Antibody Aggregates and Fragments on UHPLCand HPLC Systems with 2.5 µm BEH ParticlesStephan M. Koza and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■A performance comparison of Waters™Size-exclusion chromatography (SEC) has been...
Key words
hmws, hmwsvhmws, vhmwsigg, iggaggregates, aggregatessize, sizeexclusion, exclusionfragments, fragmentsmonomer, monomerseparations, separationsantibody, antibodydispersion, dispersionsec, secthroughput, throughputhigh, highefficiencies
Application NoteComparison of Sub-3-µm HP-SEC Columnsfor the Analysis of IgG Antibody Aggregates(HMWS) and Fragments (LMWS)Stephan M. Koza, Weibin ChenWaters CorporationThis is an Application Brief and does not contain a detailed Experimental section. AbstractThe performance of the Waters BioResolve SEC mAb, 200...
Key words
sec, secmab, mabcetuximab, cetuximabbioresolve, bioresolvecolumn, columnigg, iggmonomer, monomertailing, tailingdispersion, dispersionseparation, separationusp, usperbitux, erbituxwhile, whilehas, haspossessed
 

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Application NoteHigh Resolution Size-ExclusionChromatography Separations of mAbAggregates, Monomers, and Fragmentsusing BioResolve SEC mAb Columns onUPLC, UHPLC, and HPLC ChromatographySystemsPamela C. Iraneta, Stephan M. KozaWaters CorporationFor research use only. Not for use indiagnostic procedures. AbstractWaters BioResolve SEC mAb, 200 Å, 2.5 µm...
Key words
mab, mabdispersion, dispersionvariant, variantbioresolve, bioresolvesec, secsize, sizebio, bioacquity, acquitysystem, systemwaters, watersuplc, uplcresolution, resolutionseparations, separationsaggregates, aggregatescolumn
Impact of LC System Dispersion on the Size-Exclusion ChromatographyAnalysis of Monoclonal IgG Antibody Aggregates and Fragments:Selecting the Optimal Column for Your MethodStephan M. Koza, Corey E. Reed, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■An educational and systematicHistorically, native size-exclusion...
Key words
hmws, hmwsdispersion, dispersionaggregates, aggregatesigg, iggexclusion, exclusionantibody, antibodyacquity, acquitybio, biominutes, minutesimpact, impactsize, sizemonomer, monomersystem, systemmonoclonal, monoclonaluplc
High Resolution and High Throughput Size-Exclusion ChromatographySeparations of IgG Antibody Aggregates and Fragments on UHPLCand HPLC Systems with 2.5 µm BEH ParticlesStephan M. Koza and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■A performance comparison of Waters™Size-exclusion chromatography (SEC) has been...
Key words
hmws, hmwsvhmws, vhmwsigg, iggaggregates, aggregatessize, sizeexclusion, exclusionfragments, fragmentsmonomer, monomerseparations, separationsantibody, antibodydispersion, dispersionsec, secthroughput, throughputhigh, highefficiencies
Application NoteComparison of Sub-3-µm HP-SEC Columnsfor the Analysis of IgG Antibody Aggregates(HMWS) and Fragments (LMWS)Stephan M. Koza, Weibin ChenWaters CorporationThis is an Application Brief and does not contain a detailed Experimental section. AbstractThe performance of the Waters BioResolve SEC mAb, 200...
Key words
sec, secmab, mabcetuximab, cetuximabbioresolve, bioresolvecolumn, columnigg, iggmonomer, monomertailing, tailingdispersion, dispersionseparation, separationusp, usperbitux, erbituxwhile, whilehas, haspossessed
 

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