Agilent 6495C Triple Quadrupole LC/MS system
Brochures and specifications | 2019 | Agilent TechnologiesInstrumentation
The Agilent 6495C Triple Quadrupole LC/MS system combines high sensitivity, ruggedness and reliability to meet stringent demands in proteomics, food safety, environmental monitoring, clinical research and forensic workflows.
This application note assesses the 6495C platform across targeted proteomics, multi-pesticide screening, trace analysis of pharmaceuticals and personal care products (PPCPs) in water, and rigorous determination of analytical sensitivity metrics such as LLOQ and IDL.
Key components and methods used in this study:
Targeted proteomics: Achieved quantitative detection of G1F glycopeptide EEQYN(+1606.6)STYR with linear calibration (R2 > 0.998) and sub-nanogram sensitivity.
Food testing: Evaluated pesticides at 0.5 ms versus 15 ms dwell times, yielding RSD values below 4% and confident quantitation at trace levels.
Environmental water analysis: Direct injection of surface water samples enabled quantitation of PPCPs to 0.5 ng/L with calibration curves showing R2 > 0.997 and peak area RSD <10% for atrazine and diltiazem.
Performance metrics: Demonstrated LLOQ of 40 attograms on-column for verapamil and 1 femtogram for bentazon; calculated instrument detection limit (IDL) of 1.8 pg/mL for estriol based on statistical precision (RSD 11.3%).
Notable advantages include:
Emerging advances may involve further optimization of ion transmission funnels, AI-driven method development, expansion of dynamic MRM libraries, and applications in single-cell proteomics, non-targeted screening hybrid workflows and real-time environmental monitoring.
The Agilent 6495C LC/MS system delivers exceptional confidence in quantitative analyses, backed by innovative hardware, seamless software integration and validated workflows. It offers a robust platform for diverse high-impact applications demanding sensitivity, precision and operational efficiency.
Agilent Technologies Inc Agilent 6495C Triple Quadrupole LC/MS System Application Note 5991-4541EN 2019
LC/MS, LC/MS/MS, LC/QQQ
IndustriesManufacturerAgilent Technologies
Summary
Significance of the Topic
The Agilent 6495C Triple Quadrupole LC/MS system combines high sensitivity, ruggedness and reliability to meet stringent demands in proteomics, food safety, environmental monitoring, clinical research and forensic workflows.
Objectives and Study Overview
This application note assesses the 6495C platform across targeted proteomics, multi-pesticide screening, trace analysis of pharmaceuticals and personal care products (PPCPs) in water, and rigorous determination of analytical sensitivity metrics such as LLOQ and IDL.
Methodology and Instrumentation
Key components and methods used in this study:
- Agilent 6495C Triple Quadrupole LC/MS system featuring third-generation iFunnel, refined Q1 ion optics, curved collision cell, VacShield capillary maintenance and high-energy conversion dynode detector
- Agilent 1290 Infinity II LC with Poroshell 120 columns for high-efficiency separations
- Agilent Bond Elut SPE and QuEChERS protocols for sample cleanup to reduce matrix effects
- Agilent MassHunter Software suite employing dynamic MRM, triggered MRM, collision energy optimizer and extensive compound databases (pesticides, veterinary drugs)
Main Results and Discussion
Targeted proteomics: Achieved quantitative detection of G1F glycopeptide EEQYN(+1606.6)STYR with linear calibration (R2 > 0.998) and sub-nanogram sensitivity.
Food testing: Evaluated pesticides at 0.5 ms versus 15 ms dwell times, yielding RSD values below 4% and confident quantitation at trace levels.
Environmental water analysis: Direct injection of surface water samples enabled quantitation of PPCPs to 0.5 ng/L with calibration curves showing R2 > 0.997 and peak area RSD <10% for atrazine and diltiazem.
Performance metrics: Demonstrated LLOQ of 40 attograms on-column for verapamil and 1 femtogram for bentazon; calculated instrument detection limit (IDL) of 1.8 pg/mL for estriol based on statistical precision (RSD 11.3%).
Benefits and Practical Applications
Notable advantages include:
- Unmatched analytical sensitivity for trace analytes in complex matrices
- High throughput and fast MRM scheduling for multi-analyte assays
- Extended mass range to m/z 3,000 enabling large peptide and PTM analysis
- Integrated hardware and software solutions streamlining workflow efficiency
Future Trends and Opportunities
Emerging advances may involve further optimization of ion transmission funnels, AI-driven method development, expansion of dynamic MRM libraries, and applications in single-cell proteomics, non-targeted screening hybrid workflows and real-time environmental monitoring.
Conclusion
The Agilent 6495C LC/MS system delivers exceptional confidence in quantitative analyses, backed by innovative hardware, seamless software integration and validated workflows. It offers a robust platform for diverse high-impact applications demanding sensitivity, precision and operational efficiency.
References
Agilent Technologies Inc Agilent 6495C Triple Quadrupole LC/MS System Application Note 5991-4541EN 2019
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