High-Throughput In Vitro ADME Analysis with Agilent RapidFire/MS Systems: Cytochrome P450 InhibitionApplications | 2020 | Agilent Technologies InstrumentationSample Preparation, LC/TOF, LC/HRMS, LC/MS, LC/MS/MS, LC/QQQIndustriesClinical ResearchManufacturerAgilent TechnologiesKey wordsrapidfire, nadph, inhibitor, naph, midazolam, remaining, inhibition, activity, cyp, drug, azamulin, cytochrome, ritonavir, without, throughput, incubations, fda, traditional, adme, concentration, diltiazem, inhibitors, substrates, dependent, vitro, experiments, system, produces, vaughn, were, validated, chlorzoxazone, discovery, indicate, hlm, seven, probe, microsomes, miller, conventional, bupropion, termination, individual, productive, time, process, testosterone, adverse, pooled, qqq
3D spheroid culture as a tool for studying drug metabolism
2019|Thermo Fischer Scientific|Applications
APPLICATION NOTEHuman spheroid-qualified hepatocytes3D spheroid culture as a tool for studyingdrug metabolismIntroductionThe primary human hepatocyte (PHH) three-dimensional(3D) culture model developed by Thermo Fisher Scientificholds promise for disease modeling and functional studies.As the liver is the principal site of metabolism for...
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High-Throughput In Vitro ADME Analysis with Agilent RapidFire/MS Systems: Cytochrome P450 Inhibition
High-Throughput In VitroADME Analysis with AgilentRapidFire/MS Systems:Cytochrome P450 InhibitionApplication NoteAuthorsVaughn MillerAgilent Technologies, Inc.Wakefield, MA, USAIntroductionAnalysis of cytochrome P450 (CYP) inhibition is an important component of thedrug discovery process as adverse drug-drug interactions can lead to terminationof a drug development program,...
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Fragment-Based Drug Discovery: Comparing Labeled and Label-Free Screening of β-Amyloid Secretase (BACE-1) Using Fluorescence Spectroscopy and Ultrafast SPE/MS/MS
Fragment-Based Drug Discovery:Comparing Labeled and Label-FreeScreening of β-Amyloid Secretase(BACE-1) Using FluorescenceSpectroscopy and UltrafastSPE/MS/MSApplication NoteAuthorsIntroductionLauren E. Frick and William A. LaMarrAgilent Technologies, Inc.Wakeﬁeld, MA, USAFragment-based screening offers advantages over traditional high-throughputscreening by allowing more comprehensive coverage of chemical space, but thetypical...
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High-Throughput Analysis of Cytochrome P450 Inhibition in Intact Human Hepatocytes
High-Throughput Analysis ofCytochrome P450 Inhibition in IntactHuman HepatocytesApplication NoteAuthorsAbstractKari E. Schlicht and Vaughn P. MillerUltrafast methods of analysis were developed using the Agilent RapidFireAgilent Technologies, Inc.High‑Throughput Mass Spectrometry System in combination with an AgilentWakefield, MA6490 Triple Quadrupole Mass Spectrometer for...
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