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Improving IEX Technology and IEX-MS Applications for Domain Specific Charge Variant Analysis of mAb

 

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Characterization and Monitoring of mAb Charge Variants via Online IEX-MS Samantha Ippoliti Waters MS User Meeting ASMS 2019 Atlanta, GA June 1, 2019 ©2019 Waters Corporation 1 Agenda 1. mAb CQA Characterization 2. Reverse Phase vs Ion Exchange Chromatography 3.…
Key words
iex, iexvariants, variantsmab, mabcharge, chargeacidic, acidicdeamidation, deamidationvariant, variantmain, mainonline, onlineintact, intactspecieslikely, specieslikelyinfliximab, infliximabbasic, basicexchange, exchangespecies
[ APPLICATION NOTE ] Automated Analytical Cation-Exchange Chromatography Fraction Collection and Structural Characterization of Monoclonal Antibody Charge Variants Hua Yang, Bill Warren, and Stephan Koza Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Cation-exchange separation on Charge variant levels…
Key words
fraction, fractionfractions, fractionsmab, mabcharge, chargevariants, variantscex, cexscfc, scfcwfm, wfmanalytical, analyticalcollection, collectionstructural, structuraluplc, uplcacquity, acquitycharacterization, characterizationbioresolve
[ APPLICATION NOTE ] Online IEX-MS of mAb Charge Variants Using a BioResolve SCX mAb Column, IonHance CX-MS pH Concentrates, and BioAccord System Samantha Ippoliti, Andrew Schmudlach, Matthew A. Lauber, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION…
Key words
iex, iexcharge, chargemab, mabmass, massacquity, acquitybioresolve, bioresolveides, idesrda, rdabioaccord, bioaccordmain, mainacidic, acidicscx, scxelectrophoresis, electrophoresisvariant, variantvariants
Online IEX-MS Characterization and Monitoring of mAb Charge Heterogeneity Using an Optimized Cation Exchange Resin and Compact TOF Mass Spectrometer Samantha Ippoliti1, Qi Wang1, Ying Qing Yu1, Matthew A. Lauber1, Henry Shion1 1 Waters Corporation, Milford, MA, USA INTRODUCTION RESULTS…
Key words
variants, variantsiex, iexcharge, chargedeamidation, deamidationacidic, acidictrastuzumab, trastuzumabmain, mainmab, mabconformational, conformationalassignmentb, assignmentbbasic, basicnative, nativeexchange, exchangepossible, possiblemass
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