ACQUISITION MODE CHARACTERIZATION FOR THE QUANTITATIVE AND QUALITATIVE ANALYSIS OF CROSS-LINKED PEPTIDES BY TARGETED AND UNTARGETED LC-IM-MS

 

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Crosslinking mass spectrometry (XL-MS) goes mainstream
2018|Thermo Fischer Scientific|Technical notes
proteomics WHITE PAPER 65343 Crosslinking mass spectrometry (XL-MS) goes mainstream Mass spectrometrists and structural biologists guide to using XL-MS to understand the structure and function of molecular machines Author Executive summary Julian Saba, Thermo Fisher Scientific, San Jose, CA, USA…
Key words
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Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…
Key words
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evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…
Key words
buurbu, buurbucrosslinked, crosslinkedcleavable, cleavablecrosslinkers, crosslinkersdsso, dssoethcd, ethcdsequestht, sequesthtlumos, lumoscrosslinking, crosslinkingfusion, fusiondss, dssxlinkx, xlinkxorbitrap, orbitrapcid, cidpeptide
Reiko Kiyonami, Michael Senko, Vlad Zabrouskov, Andreas F.R. Hühmer, Thermo Fisher Scientific, San Jose, CA, USA Jarrett Egertson, Sonia Ting, Michael MacCoss, University of Washington, Seattle, WA, USA Appli cat i on N ote 6 0 0 Large-Scale Targeted Protein…
Key words
heavyk, heavykwisim, wisimdia, diaheavyr, heavyrcid, cidsim, simcarboxymethyl, carboxymethylorbitrap, orbitrapdata, datapeptide, peptidefusion, fusiontargeted, targetedquantified, quantifiedproteins, proteinsisolation
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