High Performance Liquid Chromatography (HPLC) is widely recognized as a premier chemical analytical technique in many industries vital for human existence. Its ability to separate and detect numerous classes of organic and inorganic compounds, as well as determine their concentration in various matrices make HPLC by far the most popular analytical tool in the world.However, its high costs, operational complexity, and the need for highly qualified specialists present significant challenges.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1242"> Alltesta™ Automated Analyzer</a>, simplifies the HPLC analysis process, making it more accessible without compromising quality or accuracy.<h4>Challenge</h4>The laboratory in question faced the challenges of:<ul><li>High costs of HPLC instrumentation and separation columns</li><li>Significant space requirements – traditional HPLC systems take up a large amount of laboratory space, limiting flexibility and accessibility.</li><li>The need for a specialized laboratory environment and employ highly educated and well-trained scientific personnel</li><li>Extensive analytical method development, even for simple analyses.&nbsp;</li></ul>These factors limit the accessibility of HPLC and restrict its use outside specialized laboratories.<h4>Solution: Alltesta™ Automated Analyzer</h4>The laboratory decided to explore SIELC’s offerings and quickly found an Alltesta™ Automated Analyzer that met their requirements. Designed to simplify HPLC workflows and address key user challenges, the system offers:<ul><li>Compact size – Alltesta™ Automated Analyzer features a space-saving design, making it ideal for small laboratories and research environments with limited workspace.</li><li>Ease of use – operates from a standard power outlet and connects to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a> via Wi-Fi, Ethernet, or a mobile network.</li><li>Automated method setup – The company SIELC removes the major burden of analytical method development from an inexperienced system operator, allowing them to focus on obtaining analytical results in minimal time. This is accomplished by SIELC’s experienced staff, who identify the optimal columns, solvents (mobile phases), and gradient parameters for a particular analysis—free of charge for clients. The company also maintains a vast library of analytical methods for numerous compounds and produces a wide variety of HPLC columns to support their implementation.</li><li>Cloud-based control and data processing – server-hosted software enables remote operation and provides analytical results in an easy-to-read format.</li><li>Advanced HPLC technologies – the system features a pulse-free syringe pump (up to 5000 psi) (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Mini Syringe Pump</a>), an autosampler (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta™ Mini-Autosampler</a>), and a multi-wavelength UV detector (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta™ Mini UV-Vis Detector</a>), ensuring reliable analytical performance.</li></ul><figure class="image"><img style="aspect-ratio:1567/972;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg" alt="Bioanalytic: SIELC Alltesta™ Gradient Automated Analyzer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg 245w," sizes="100vw" width="1567" height="972"></figure><h4>Results &amp; Impact&nbsp;</h4>The implementation of Alltesta Automated Analyzer has significantly streamlined the HPLC analysis process. Laboratory report:<ul><li>Reduced costs by minimizing expenses on consumables and cutting method development time.</li><li>Simplified workflow – even individuals without specialized training can perform analyses and obtain accurate results.</li><li>System reliability – extensive testing has shown no hardware or software failures.</li><li>Enhanced data security – HPLC.cloud ensures safe data storage, reliable processing, and secure remote access.&nbsp;</li></ul>Dr. Michael Z. Kagan, PhD, Senior Chromatographer with 28 years of experience at Pfizer, AbbVie, and Coca-Cola, highlights:&nbsp;"During six months of testing, I did not encounter a single hardware or software issue. The system is easy to use, offers flexible column and method configurations, and benefits from expert chromatographic support, making Alltesta a reliable and user-friendly solution."<h4>Conclusion</h4>Alltesta™ Automated Analyzer removes the main barriers associated with HPLC analysis, making it accessible, simple, and efficient. With automation and cloud-based technologies, users can focus on obtaining results rather than dealing with complex instrument settings.

Optimizing HPLC Analysis with Alltesta™ Automated Analyzer

SIELC has developed the first and only HPLC software that is accessible via the cloud - <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a>.<h4>&nbsp;Special Features:</h4><ul><li>Cloud Integration: Real-time data streaming and storage on HPLC.cloud ensure your experiment data is always backed up and accessible.</li><li>Remote Access: Control and monitor your system from anywhere — lab, office, or on the go — using a PC, tablet, or phone.</li><li>Data Security: All your results are stored in a centralized, secure location — so no data is ever lost.</li><li>Sync Capabilities: Chromatograms are synced with HPLC.cloud every second, providing real-time experiment updates.</li></ul><h4>Key Functionality:</h4><ul><li>Real-time Chromatogram Streaming: Watch live data as it’s being collected.</li><li>Sequence Building and Recording: Build, record, and store experiment sequences.</li><li>Method Storage: Save and reuse methods for consistency and efficiency.</li><li>Data Accessibility: Store all your sample and result data securely in the cloud, accessible anytime.</li><li>Remote Control: Start, stop, or modify analyses from any internet-connected device.</li><li>Comprehensive Data Analysis: Calibrate, analyze, and quantify directly within the cloud interface.</li><li>Mobile and PC Access: Use on PC, tablet, or smartphone for maximum flexibility.</li><li>Online Data Search: Easily search and access your data from anywhere.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg 225w," sizes="100vw" width="801" height="554">No software installation. No USBs. No data loss.

How to Use HPLC Cloud - A Complete Guide

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Bioanalytic started its operations in 1998. The company was born from a passion for science and the need to provide innovative solutions in the laboratory equipment market, from a small business to a dynamically developing company with an established position.

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HPLC Cloud Software Set-up Manual

Innovative research and development company manufacturing mixed-mode HPLC columns, HPLC Analyzers, OEM parts for HPLC, fittings.

SIELC

SIELC HPLC Software - HPLC.cloud

SIELC has developed the first and only HPLC software that is accessible via the cloud.

<h5>Each <a href="https://lcms.labrulez.com/products?instrumentationItems=27&amp;search=SIELC">instrument</a> is connected directly to our secure cloud server via an Ethernet line.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>All instrument parameters are visible and adjustable on the fly from any internet-enabled device. Instrument settings are stored as methods, which can be retrieved and used for specific compounds.</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_2_f626a8f395.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
<h5>Multiple samples can be organized in a sequence with various methods. Results can be compared and quantitated:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_3_6eb75a29c9.jpg" alt="SIELC: SIELC Software – HPLC.cloud."/>
Please see an online example of the separation results <a href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.
<h5>With your Alltesta and HPLC.cloud free subscription, you can analyze almost any compound from the palm of your hand. For example, you can analyze the dyes that are in your favorite purple sports drink:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_4_b8e9abd33a.jpg" alt="SIELC: Separation of Blue 1 and Red 40 dyes in Gatorade Zero – Grape."/>
<h5>Alternatively, you can analyze the caffeine content in your morning coffee:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_5_f39557543e.jpg" alt="SIELC: Retention of Caffeine."/>
<h5>Another option is to separate and analyze the vitamin content in your daily multi-vitamin:</h5>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_6_bbf8e002a3.jpg" alt="SIELC: Separation of Vitamin C and Vitamin B2."/>

SIELC HPLC Software - HPLC.cloud

Each instrument is connected directly to our secure cloud server via an Ethernet line.

All instrument parameters are visible and adjustable on the fly from any internet-enabled device. Instrument settings are stored as methods, which can be retrieved and used for specific compounds.

Multiple samples can be organized in a sequence with various methods. Results can be compared and quantitated:

With your Alltesta and HPLC.cloud free subscription, you can analyze almost any compound from the palm of your hand. For example, you can analyze the dyes that are in your favorite purple sports drink:

Alternatively, you can analyze the caffeine content in your morning coffee:

Another option is to separate and analyze the vitamin content in your daily multi-vitamin:

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How to Use HPLC Cloud - A Complete Guide

Optimizing HPLC Analysis with Alltesta™ Automated Analyzer

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