Bioanalytic QTRAP vs QTOF Quality analysis training

BASIC INFORMATION

• TITLE: QTRAP vs QTOF Quality analysis training
• CATEGORY: LC-MS/MS training
• LOCATION: Lodz, PL
• TRAINING TIME: 3 days
• NUMBER OF PARTICIPANTS: up to 5 people
• EQUIPMENT: SCIEX QTRAP & QTOF LC-MS/MS

Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training.

TRAINING COURSE DESCRIPTION

QTRAP vs QTOF qualitative analysis - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of DDA (IDA) (QTRAP & QTOF) and DIA (SWATH) (QTOF) methods. Analysis and comparison of results will be conducted using SciexOS, MarkerView, LightSight and MoleculeProfiler software.

Training program

Day I

Theoretical part

• Principle of operation of MS/MS systems
• Differences and capabilities of QTRAP and QTOF
• Comparison of typical analytical procedures and their application
• DDA (IDA) vs DIA (SWATH) methodology.
• Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView
• General principles of compound fragmentation

Day II

Objective - to develop methods to search for metabolites of a known substance.

QTRAP

• Development of IDA screening methods (pMRM, Prec/NL, EMS)
• Optimization of LC-MS/MS IDA method
• Data acquisition

QTOF

• Development and optimization of LC-MS/MS IDA and SWATH methods
• Data acquisition

Day III

Objective - identification and profiling of metabolites of a known substance

QTRAP data analysis

• Manual search and identification
• LightSight, MarkerView

QTOF data analysis

• Search and data analysis methods
• Manual search and identification
• IDA vs SWATH
• Automated search and identification - SciexOS, databases
• Molecule Profiler, MarkerView

Summary and discussion