Bioanalytic QTOF quantitative and qualitative analysis training

BASIC INFORMATION

• TITLE:  QTOF quantitative and qualitative analysis training
• CATEGORY: LC-MS/MS training
• LOCATION: Lodz, PL
• TRAINING TIME: 3 days
• NUMBER OF PARTICIPANTS: up to 5 people
• EQUIPMENT: SCIEX QTOF LC-MS/MS

Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training.

TRAINING COURSE DESCRIPTION

Qualitative and quantitative analysis of QTOF - the training is designed for laboratory personnel using high-resolution (HR) LC-MS/MS systems for qualitative and quantitative analysis. The following topics are covered during the training:

• QTOF spectrometer calibration
• Targeted and untargeted methods
• Quantitative analysis (TOF MS, MRMhr)
• Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology
• Software and its use in high-resolution data analysis
• Reporting of results

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.

Training program

Day I

Theoretical part

• The principle of operation and capabilities of the QTOF system
• Comparison of typical analytical procedures and their application
• Targeted and quantitative analysis (TOF MS vs MRMhr)
• DDA (IDA) vs DIA (SWATH) methodology.
• Supporting software - Molecule Profiler, ACD Labs, MarkerView
• General principles of compound fragmentation

Day II

Objective - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.

Practical part:

• Creation and optimization of the MRMhr targeted method
• Creation and optimization of a non-targeted method IDA
• Creation and optimization of the non-targeted method SWATH
• Data acquisition (overnight)

Day III

Objective - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.

Practical part:

• Quantitative analysis - TOF MS vs MRMhr
• Methods for searching and analyzing data
• IDA vs SWATH data
• Manual search and identification
• Automated search and identification - SciexOS, databases
• Molecule Profiler, MarkerView
• Summary and discussion