Phenomenex Luna C18(2) HPLC Columns

The Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface, resulting in a C18 HPLC column with a very hydrophobic stationary phase that offers great methylene selectivity. Luna C18(2) columns enable higher flow rates while maintaining excellent resolution and efficiency. The advanced packing technology ensures consistent, high-performance results across both existing and newer HPLC instrumentation.

Features of Luna C18(2) columns:

• Extensive line of rugged particle sizes
• Easy method scalability from HPLC to preparative and bulk purification LC
• C18(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L1)
• Faster separations
• No need for time-consuming method revalidation
• An ideal choice for improving lab productivity and efficiency

Phenomenex: Luna C18(2) HPLC Column

• Carbon Load: 17.5, 17.5 %
• Surface Area (m²/g): 400
• Particle Size: 2.5, 3, 5, 10, 15
• Recommended Use: Recommended for improved methylene selectivity for USP methods or general LC method development

Long Column Lifetimes and Excellent Performance

Ultra-pure, metal-free silica (99.99 % purity) is the back-bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Wide pH Stability

Luna C18(2) columns are renowned for their exceptional pH stability, offering a wide operational range from pH 1.5 to 9.0. This extended pH range is achieved through the high density of the bonded phase. As a result, peak shapes remain sharp and consistent across varying pH levels, ensuring reliable performance. The wide pH stability of Luna C18 columns allows analysts to work above the pKb of basic compounds, rendering them more hydrophobic. This enhances retention, improves peak shape, and boosts reproducibility, making Luna C18(2) an ideal choice for flexible and long-lasting HPLC methods.

Mobile Phase Compatibility

When using HPLC columns, it's crucial to use HPLC-grade solvents and materials while avoiding immiscible solvent/buffer combinations to prevent column damage. To ensure the highest performance, solvent filtration is strongly recommended to eliminate trace impurities from your chosen mobile phase.

All Luna HPLC columns, including the widely used Luna C18(2), are stable in 100% organic conditions, offering flexibility in method development. It is essential to keep the mobile phase pH within the recommended limits for each stationary phase to avoid compromising the column's integrity and lifespan. Adhering to these guidelines ensures optimal column performance, extended longevity, and consistent, reliable results in your chromatographic analyses.

Common Applications:

Pharmaceutical Compounds:

• Pain Relievers & Anti-inflammatory: Acetaminophen, Aspirin, Ibuprofen, Naproxen, Cortisone Acetate, Prednisone (oral solution)
• Stimulants & Decongestants: Caffeine, Pseudoephedrine, Phenylephrine Hydrochloride, Phenylpropanolamine
• Respiratory & Allergy Medications: Albuterol, Diphenhydramine, Guaifenesin, Dextromethorphan
• Antibiotics & Antimicrobials: Amoxicillin, Cephalexin, Cephradine (capsules), Chloramphenicol, Sterile Imipenem
• Cardiovascular & Blood Pressure Medications: Atenolol, Dopamine, Minoxidil, Reserpine, Procainamide
• CNS & Psychiatric Medications: Amitriptyline Hydrochloride, Imipramine, Lorazepam, Propoxyphene
• Local Anesthetics: Lidocaine
• Hormonal Treatments: Estradiol

Stationary phase Luna C18(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative

Luna C18(2) HPLC Microbore, Minibore, MidBore, Analytical and Semi-preparative Columns are available with these parameters:

Microbore Columns

• Column length (mm): 50, 150, 250
• Column ID (mm): 1
• Particle size (µm): 3, 5

Minibore Columns

• Column length (mm): 30, 50, 100, 150, 250
• Column ID (mm): 2
• Particle size (µm): 3, 5

MidBore Columns

• Column length (mm): 30, 50, 150, 250
• Column ID (mm): 3
• Particle size (µm): 3, 5

Analytical Columns

• Column length (mm): 30, 50, 75, 100, 150, 250
• Column ID (mm): 4.6
• Particle size (µm): 3, 5

Semi-Prep Columns

• Column length (mm): 250
• Column ID (mm): 10
• Particle size (µm): 5

Phenomenex Luna HPLC Columns

Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.

All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.

Explore Luna Columns

• Proven Performance: Extensive line of rugged USP and Ph.Eur. phases
• Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance
• Ultra-High Purity: Metal free silica (99.99% purity)  ensures a low activity surface and less secondary interactions
• Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification

Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.

Phenomenex - Phases of Luna Columns