KNAUER Eurospher II 100 C18A (U)HPLC Columns (USP L1)

C18A columns for polar reversed phase applications with 100 % aqueous mobile phase (USP L1)

C18 stationary phase for the analysis of more polar and hydrophilic compounds in reversed phase mode (USP L1). Silica gel based columns with C18 modification with hydrophilic endcapping. Because of their modification, C18A phases can be operated under 100 % aqueous conditions without destroying the stationary phase, what is not recommended for classical C18 phases caused by their hydrophobicity.

Properties

• Separation mechanism: Hydrophobic and polar interaction
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Polar, monomeric C18 (Octadecyl) modification, polar endcapping, with 10 % carbon content (~ 50 % endcapping).

Key features

• C18 A phase with alternative, more polar selectivity, suited for the use with pure aqueous mobile phases, 50 % endcapping and resulting 10 % carbon content, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications

Recommended application areas

• Polar endcapped C18 phase for water soluble and polar analytes, 100 % aqueous eluents for analysis of very polar compounds:
  • basic pharmaceutical ingredients
  • water soluble vitamins
  • catecholamines
  • as well as organic acids.

KNAUER: C18A

Tip: With an endcapping the influence of nonderivatized silanol groups can be minimized. The endcapping reagent is generally a smaller silane than used for derivatisation. This treatment reduces the unwanted interaction of polar or charged analytes (acids, bases ect.) because the amount of available silanol groups is reduced.

Stationary phase Eurospher II 100 C18A is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 C18A Analytical Columns are available with these parameters:

• Column length (mm): 50, 100, 150, 250
• Column ID (mm): 2, 3, 4, 4.6
• Particle size (µm): 2, 3, 5

Eurospher II 100 C18A Semi-Preparative Standard Columns are available with these parameters:

• Column length (mm): 30, 50, 125, 150, 250
• Column ID (mm): 8, 16
• Particle size (µm): 5, 10

KNAUER (U)HPLC Columns

Widest range of HPLC and UHPLC columns for all separation modes and applications.

• Safe Investment: Robust and reliable columns for attractive price 
• Separation Mode: Reversed Phase, HILIC, Normale
• Simplicity: Easy purchase via our online store with fast delivery
• Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
• Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar

KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.

KNAUER: KNAUER (U)HPLC Columns