KNAUER Eurospher II 100 Diol (U)HPLC Columns for NP (USP L20)

Diol columns for Normal Phase in HPLC and UHPLC (USP L20)

Diol packing material can be universally used in different polar modes (NP, RP and HILIC). Diol bonded phases are a versatile alternative to unbonded silica for normal phase separations with shorter equilibration times.

The hydroxyl groups provide good selectivity without excessive retention, caused by the weaker H-bonding of the diol layer than of silanols. Some diol bonded phases have been developed specifically for applications in the HILIC mode.

Properties

• Separation mechanism: Hydrophilic interaction
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Diol, no endcapping, with 5 % carbon content 

Key features

• High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical Diol phase without endcapping and 5 % carbon content, outstanding mechanical and chemical stability.

Recommended application areas

• Alternative to the silica packing with shorter equilibration time and comparable selectivity; due to the lower activity of these packings it can be also used for SEC-applications.

KNAUER: Diol

Tip: The most important column parameters for evaluation are retention time, peak symmetry and theoretical plates. Retention time to prove the reproducibility of
measurements. Peak symmetry to estimate how good the column bed is packed (peak symmetry value of 1.0 is optimal). Theoretical plate numbers to determine the effectiveness of the HPLC column.

Stationary phase Eurospher II 100 Diol is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 Diol Analytical Columns are available with these parameters:

• Column length (mm): 50, 100, 150, 250
• Column ID (mm): 3, 4, 4.6
• Particle size (µm): 3, 5

Eurospher II 100 Diol Semi-Preparative Standard Columns are available with these parameters:

• Column length (mm): 30, 50, 125, 150, 250
• Column ID (mm): 8, 16
• Particle size (µm): 5, 10

KNAUER (U)HPLC Columns

Widest range of HPLC and UHPLC columns for all separation modes and applications.

• Safe Investment: Robust and reliable columns for attractive price 
• Separation Mode: Reversed Phase, HILIC, Normale
• Simplicity: Easy purchase via our online store with fast delivery
• Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
• Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar

KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.

KNAUER: KNAUER (U)HPLC Columns