KNAUER Eurospher II 100 NH2 (U)HPLC Columns for NP & HILIC (USP L8)

Amino columns for Normal Phase and for HILIC applications (USP L8)

Amino (NH₂) bonded silica gel phases can be used in the normal phase (NP) and HILIC mode, but also for reversed phase (RP) mode. They show alternative normal phase selectivity to unbonded silica, especially for aromatics. Furthermore, the selectivity is influenced by hydrogen bond interactions with the analyte. Amino columns can also be used in the HILIC mode for carbohydrate analysis and for other polar compounds. Their weak anion-exchange properties can be used in the analysis of anions and organic acids.

The amino group is bonded to the silica support via a propyl linker.

Properties

• Separation mechanism: Hydrophilic and ionic interactions
• Ultra pure, spherical high performance HPLC phase based on silica gel
• Amino modification (Aminopropyl)
• 4 % carbon content (without endcapping)

Key features

• Multi mode column for RP, NP, HILIC and IC, Aminopropyl modification without endcapping, outstanding mechanical and chemical stability.

Recommended application areas

• Most flexible phase in the Eurospher II family; can be used in three modes: normal phase, reversed phase and ion chromatography mode (weak anion exchanger); different selectivity to the silica packing; in reversed phase mode mainly used for analysis of carbohydrates. 

KNAUER: NH2

Tip: A precolumn can help to protect your analytical column. Especially when working with highly matrix afflicted samples it is highly recommended to use precolumns.

Stationary phase Eurospher II 100 NH2 is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 NH2 Analytical Columns are available with these parameters:

• Column length (mm): 50, 100, 150, 250
• Column ID (mm): 3, 4, 4.6
• Particle size (µm): 3, 5

Eurospher II 100 NH2 Semi-Preparative Standard Columns are available with these parameters:

• Column length (mm): 30, 50, 125, 150, 250
• Column ID (mm): 8, 16
• Particle size (µm): 5, 10

KNAUER (U)HPLC Columns

Widest range of HPLC and UHPLC columns for all separation modes and applications.

• Safe Investment: Robust and reliable columns for attractive price 
• Separation Mode: Reversed Phase, HILIC, Normale
• Simplicity: Easy purchase via our online store with fast delivery
• Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
• Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar

KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.

KNAUER: KNAUER (U)HPLC Columns