KNAUER Eurospher II 100 Si (U)HPLC Columns for NP & HILIC (USP L3)

Si columns for Normal Phase and HILIC applications (USP L3)

Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP). They are still used in HPLC, although reversed phase (RP) is by far the most often used HPLC mode today.

A wide range of porous Si materials are available, characterized by:

• Surface area
• Pore size
• Particle size
• Purity
• Metal content

Properties

• Separation mechanism: Hydrophilic interactions
• Ultra pure, spherical high performance HPLC phase based on silica gel
• No modification, no endcapping, with 0 % carbon content

Key features

• High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, outstanding mechanical and chemical stability.

Recommended application areas

• Wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC and HILIC; good choice for analytical and preparative purposes to separate polar compounds.

Tip: Normal phase (NP) stationary phases have been the first phases for chromatography and that is why their properties of polarity were determined as ’’normal’’. The stationary phase of a NP column has polar properties and is commonly used with nonpolar solvents as hexane or heptane. The other way round a reversed phase (RP) column has covalent charcteristics and is used with more polar eluents, like mixtures of acetonitrile and water.

Stationary phase Eurospher II 100 Si is provided in these column types: analytical, semi-preparative and preparative.

Eurospher II 100 Si Analytical Columns are available with these parameters:

• Column length (mm): 50, 100, 150, 250
• Column ID (mm): 3, 4, 4.6
• Particle size (µm): 3, 5

Eurospher II 100 Si Semi-Preparative Standard Columns are available with these parameters:

• Column length (mm): 30, 50, 125, 150, 250
• Column ID (mm): 8, 16
• Particle size (µm): 5, 10

KNAUER (U)HPLC Columns

Widest range of HPLC and UHPLC columns for all separation modes and applications.

• Safe Investment: Robust and reliable columns for attractive price 
• Separation Mode: Reversed Phase, HILIC, Normale
• Simplicity: Easy purchase via our online store with fast delivery
• Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
• Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar

KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.

KNAUER: KNAUER (U)HPLC Columns