KNAUER Eurosil Bioselect 300 C4 HPLC Columns (USP L26)

Analytical wide pore phase for the determination of biomolecules bigger than 20 kDa

Eurosil Bioselect C4 has been specifically developed for the determination and purification of peptides, proteins, and oligonucleotides up to a molecular weight of approximately 100 kDa. The optimized manufacturing process guarantees excellent batch–to-batch reproducibility. The Eurosil Bioselect sorbent is the first choice for Biochromatography with silica material.

Properties

• Ultra pure, spherical wide pore HPLC phase based on wide pore silica gel
• Unpolar, monomeric C4 modification, endcapped
• 2 % carbon content (conventional endcapping)
• Separation mechanism: hydrophobic and hydrophilic interaction

KNAUER: C4

Recommended application areas

• Hydrophilic biomolecules and especially peptides larger than 10 000- 20 000 Da

KNAUER: Eurosil Bioselect - Phases and recommended sizes of molecules

KNAUER Wide pore HPLC columns

For enhanced resolution, capacity and recovery of proteins and other biomolecules

• Material: Silica or polymer particles
• Separation Ranges: 2–100 kDa
• Column Brands: Eurosil Bioselect or AppliChrom®
• Particle sizes: 3–10 µm
• Pore Sizes: 300 or 100–1000 Å
• Specific surface: 90 ± 5 m2/g
• Pore volume: 0.8 ml/g
• Density: 450 g/l
• Applications: Peptides exceeding approximately 50 amino acids and oligonucleotides greater than 25 residues
• Customize: Bioanalysis requires a lot of flexibility in the pore size, the pH range, the temperatures and the solvents used. To achieve this, we cross phase limits.

Characteristics 

• Eurosil Bioselect columns are available in 4 modifications, useful for a wide range of separation tasks involving large molecules up to 100 000 Da.
• Strict manufacturing procedures ensure a very narrow pore size distribution.
• Interfering anions or heavy metal ions on the silica surface are not present in Eurosil Bioselect columns because only ultra pure silica gel and high quality chemical modifications are employed.

KNAUER: Eurosil Bioselect

Separation mechanism of Eurosil Bioselect

To separate larger molecules, they need to freely access the interior of the pores of the packing material. Therefore, the analytes’ diameter must be smaller than the average pore diameter. 

For higher molecular weight solutes, the use of lower pore size materials (60-120 Å) as typically used for the analysis of small molecules may result restricted diffusion in the material´s pores and therewith reduced column efficiency. 

Modifications

The recommended modification of Eurosil Bioselect depends on the molecular weight of the analytes.

KNAUER: Eurosil Bioselect - chemical modifications