AppliChrom® GPC columns for GPC analysis of organic molecules with THF, toluene, chloroform
Properties:
- Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution
- High pressure stability: up to 150 bar
- Temperature stable: between 10-145 °C
- GPC range: from 0.1 up to 50 000 kDa
- Low column bleed for low detector noise
- High purity to eliminate adsorptive effects and combine pure GPC with long lifetime
- Large calibration ranges for several decades due to the unique process of BPT technology
- Range of individual porosities that can be used individually or in combination
- Optimized for: 1 ml/min flow rate for 8 mm ID columns
- High level of reproducibility and peak precision due to the advanced packing procedure
- Recommended eluents: THF, Toluene, CHCl₃, dichlorbenzene, hexane/EtOAc (10:90, V:V)
KNAUER: AppliChrom® ABOA StyDiViBe-P - Molecular sizes range
Applications
Hydrophobic oligomers or polymers like:
- PVC, PMMA, polystyrene, epoxy resins, polyesters, bisphenol A resins
GPC & SEC Columns
GPC & SEC systems offer solutions for various applications and laboratories
Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.
Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a wide range of pore sizes (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and several particle sizes (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.
KNAUER: Size Exclusion - principle
KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications
- AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.
- AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,
PEG,.... - AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)
- AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...
- AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.
- Seperation Ranges: 100–50 000 000 Da
You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:
Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).
KNAUER: AppliChrom® ABOA columns.
