Uncover and confidently characterize proteoforms within complex matrices, including proteoforms containing truncations, alternative splices, and post-translationally modified proteins.
Extensive searches make use of various fragmentation methodologies (e.g., ETD, HCD, UVPD), allowing you to make full use of the high-quality MS/MS data obtained when searching against the full biological complexity present in UniProt. With Thermo Scientific ProSightPD software, see what is really there and make the most of your data.
Utilize several search modes for identifying intact and truncated proteins. ProSightPD software offers the ability to search entire raw files or quickly select specific MS2 scans for further searching. Information in UniProt enables the identification of modified or processed proteins.
Import result files from Proteome Discoverer software or entire proteomes via UniProt formatted XML and FASTA files with Database Manager. Fully customize imported databases using thr ProSight Annotator GUI to characterize PTMs, disulfide bonds, mutations and truncations.
Analyze large datasets with many data files using ProSightPD nodes for Proteome Discoverer software. Create multi-step searches and utilize built in results interpretation tools.
Different database search modes allow you to optimize data interrogation based upon your experimental needs. Searches are driven through the use of a node-based system allowing flexibility of experimental design.
Thermo Scientific™ ProSightPD Software: ProSightPD Database Searching.
Further information relating to the two search modes, Absolute Mass Search and Biomarker Search.
Thermo Scientific™ ProSightPD Software: Complementary Search Types: Digging Deeper in Complex Samples.
Confident proteoform identification is realized through the use of XML-formatted databases, ensuring that nothing is missed.
Thermo Scientific™ ProSightPD Software: Database Manager: Add remove isoforms and PTMs.
Improvements to data visualization ensure that you can better interact with and interpret your data, minimizing the time taken to deliver actionable results.
Thermo Scientific™ ProSightPD Software: LFQ Visualizations.
Top-Down Workflows: ProSightPD includes templates to easily build the most commonly used-top down data analysis workflows.
Thermo Scientific™ ProSightPD Software: ProSightPD 2.0: Tailored Workflows for All Types of Data.
