Thermo Scientific Acclaim Trinity P1 HPLC Columns

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity.

The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance.

Superior Performance for Pharmaceutical Applications

• Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes)
• Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents
• Distinctive spatial separation of the anion-exchange and cation-exchange regions
• Flexible method development with each retention mechanism controlled independently
• Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD)
• Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths

Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.

Specifications

• For use with: ELSD, UV Detector
• Max. Pressure: 4500 psi (310 bar)
• Packing Material: Spherical, Nanopolymer Hybrid Silica
• Stationary phase: Reversed Phase/Anion Exchange/Cation Exchange
• Surface Area: 100 m²/g
• Temperature: 45°C
• Phase: Reversed Phase and Ion Exchange
• Pore Size: 300 Å
• pH: 2.5 to 7

About the Acclaim Family of HPLC Columns

Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.

• Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs
• Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations

Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns