Hybrid technology is becoming increasingly important in chromatography and for good reason. Modern analytical laboratories face complex molecules, demanding conditions, and the need for robust, reproducible LC methods. <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18 from GL Sciences</a> was developed as a response to this trend, combining the proven quality of the Inertsil series with the new possibilities offered by hybrid organic–inorganic silica materials. Read on to learn what makes hybrid columns unique and what advantages they bring.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_GL_hybrid_uvod_AJ_5b1fd0e166.jpg" alt=" Watrex: Special promotion on new Inertsil Hybrid-C18 columns from Watrex Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_GL_hybrid_uvod_AJ_5b1fd0e166.jpg 245w," sizes="100vw" width="1600" height="900"><h4>Hybrid Technology: A New Level of Stability&nbsp;</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18</a> is a reversed-phase C18 column engineered with hybrid silica gel that combines inorganic silica with organic components. This construction provides exceptional chemical stability.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_GL_obr_2_afb06cdb89.jpg" alt="Watrex: Hybrid Technology: A New Level of Stability&nbsp;" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_GL_obr_2_afb06cdb89.jpg 245w," sizes="100vw" width="1600" height="522"><h5>Key Features&nbsp;</h5><ul><li>pH range 1–12 – stable performance under both acidic and basic conditions&nbsp;</li><li>Compatible with LC/MS and LC/MS/MS&nbsp;</li><li>High durability and long lifetime&nbsp;</li><li>Low bleed suitable for sensitive detection&nbsp;</li><li>USP L1 (C18) classification&nbsp;</li></ul>Thanks to these characteristics, the column performs reliably across a wide range of applications where conventional silica-based materials may fail.&nbsp;<blockquote><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">View the full column offering HERE&nbsp;</a></blockquote><h4>A Legacy of Quality: From Silica Synthesis to the Final Column&nbsp;</h4>GL Sciences manufactures Inertsil columns entirely in-house, from the synthesis of the base silica to the final packing. This vertically integrated process, established in 1994, ensures:<ul><li>outstanding quality,&nbsp;</li><li>minimal lot-to-lot variability,&nbsp;</li><li>reliability and consistency across production batches.&nbsp;</li></ul><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18</a> was developed for users who require high durability, a wide operational pH range, and stable performance even under strongly basic conditions.&nbsp;<h4>Impact of pH on Selectivity: Stability Across the Entire pH Range&nbsp;</h4>Changes in mobile-phase pH significantly influence analyte retention and selectivity. With conventional columns, operating outside the optimal pH range often results in degradation, loss of efficiency, or inconsistent retention times.&nbsp;The hybrid material used in <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18</a>, however, enables:&nbsp;<ul><li>stable chromatographic performance,&nbsp;</li><li>excellent reproducibility,&nbsp;</li><li>high efficiency even during long-term operation.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_GL_obr_3_0b5faf00f7.jpg" alt="Watrex: Impact of pH on Selectivity" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_GL_obr_3_0b5faf00f7.jpg 245w," sizes="100vw" width="1600" height="747"><h4>Exceptional High-pH Durability (pH 11.5)&nbsp;</h4>Basic mobile phases are typically destructive for traditional C18 columns. Hybrid technology significantly improves resistance to alkaline conditions.&nbsp;<h5>Advantages at pH 11.5</h5><ul><li>no loss of theoretical plates&nbsp;</li><li>preserved peak shapes&nbsp;</li><li>no silica dissolution&nbsp;</li><li>reliable separation even during long-term exposure&nbsp;</li></ul>This durability makes it possible to develop methods that would be difficult or impractical to run on conventional silica columns.&nbsp;<h4>Outstanding Lot-to-Lot Reproducibility&nbsp;</h4>Three tested production lots of <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18</a> showed virtually identical retention times and peak shapes. As a result:&nbsp;<ul><li>method robustness is ensured&nbsp;</li><li>revalidation when switching lots is unnecessary&nbsp;</li></ul><h4>Broad Application Range&nbsp;</h4>The column delivers excellent peak shapes for:&nbsp;<ul><li>basic compounds (without tailing)&nbsp;</li><li>acidic compounds (stable retention)&nbsp;</li><li>metal-coordinating analytes (no unwanted interactions with metal surfaces)&nbsp;</li></ul>This makes it an ideal choice for pharmaceutical, bioanalytical, and chemical laboratories.<h4>Hardware Options&nbsp;</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18</a> is available in:&nbsp;<ul><li>stainless steel (SS) hardware&nbsp;</li><li>bioinert PEEK hardware (ideal for sensitive applications and LC/MS)&nbsp;</li><li>custom dimensions, including preparative formats&nbsp;</li></ul>The wide selection of configurations allows the column to be tailored precisely to the needs of any application.&nbsp;<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/32431">Download new brochure HPLC, LC/MS Columns Inertsil Hybrid-C18</a></blockquote><h4>Conclusion: Why Choose Inertsil Hybrid-C18&nbsp;</h4><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/kategorie-produktu/chromatography/hplc-columns/gl-sciences-columns/inertsil/inertsil-hybrid-c18/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Inertsil Hybrid-C18 </a>is a modern column designed for today’s analytical challenges. It offers robustness, versatility, and the long-established reliability appreciated by users of the Inertsil series. If you are considering optimizing existing methods, switching to more durable columns, or upgrading chromatographic performance, now is the perfect time.&nbsp;<blockquote>Watrex Prague has prepared a special offer! Until the end of December 2025, we are offering an exclusive 25% discount&nbsp;on all Inertsil Hybrid-C18 columns.&nbsp;</blockquote>Visit our website <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">www.watrex.com</a> to browse the full range, access technical specifications, and place orders. If you have any questions, feel free to contact us. As the official distributor of GL Sciences, we are happy to assist you with product selection and technical support. For a non-binding quotation, contact us at watrex@watrex.com.

Inertsil Hybrid-C18: Stability Across a Wide pH Range

A team of researchers at IOCB Prague headed by Dr. Tomáš Slanina has developed a new method for labeling molecules with fluorescent dyes that surpasses existing approaches in both precision and stability. The new fluorescent label remains covalently bonded to its target molecule and does not fall apart even under demanding conditions inside living cells. This allows scientists to track labeled molecules over long periods with high reliability – an advantage for research in biology, chemistry, and medicine. The study was published in Angewandte Chemie International Edition.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Tomas_Bellon_UOCHB_Dr_Tomas_Slanina_vedouci_vedecke_skupiny_Redoxni_fotochemie_a_Lucie_Salkova_doktorandka_UOCHB_36ca9e4598.jpg" alt="Tomáš Belloň / IOCB Prague: Dr. Tomáš Slanina, head of the Redox Photochemistry research group, and Lucie Šálková, PhD student, IOCB Prague" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Tomas_Bellon_UOCHB_Dr_Tomas_Slanina_vedouci_vedecke_skupiny_Redoxni_fotochemie_a_Lucie_Salkova_doktorandka_UOCHB_36ca9e4598.jpg 234w," sizes="100vw" width="1600" height="1068">Fluorescent labeling of proteins and peptides enables scientists to determine where a molecule is located in a cell and how its behavior changes over time. Current methods, however, have clear limitations. The label often attaches to an unintended site on the molecule, potentially disrupting its structure and, in extreme cases, even its biological function. Moreover, molecules labeled in this way tend to be unstable and can easily unlabel in the cellular environment.“In our work, we applied a highly precise peptide-labeling approach that uses a two-step chemical reaction triggered by light. This allows us to create exceptionally stable fluorescently labeled molecules,” says Tomáš Slanina.The new method from IOCB Prague combines the best features of existing strategies – namely, precision and outstanding molecular stability.Fluorescent labeling proceeds in two steps. First, the dye attaches to the molecule at a precisely defined position. Then, upon exposure to light, the attached label is “glued” in place, converting a temporary bond into a permanent, chemically robust one.“We call the method Stick and Glue!" explains Lucie Šálková, the study’s first author and a PhD student. “Imagine someone dancing with a glow stick. If the stick isn’t activated, you can’t see it and they disappear into the crowd – they might even drop it. But once they snap it and fasten it to their wrist, they won’t lose it, and you can easily spot where they are. Our method works similarly. First, we attach the fluorescent dye, and only after illuminating it with light do we stabilize it – we glue it to the target molecule. From then on, the molecule can be tracked easily without the dye falling off. Unlike other methods, our glow sticks don’t end up scattered on the floor at the end of the night,” she adds.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Banka_s_fluorescencne_znacenym_glutathionem_242a93510b.jpg" alt="IOCB: The photo shows a flask containing fluorescently labeled glutathione. Glutathione is a small tripeptide and one of the most important antioxidants in cells – it helps neutralize free radicals and protects cells from oxidative stress. In our research, it served as a model molecule to test a new labeling method. While glutathione consists of three amino acids, by the end of the project, we were able to label a protein containing 583 amino acids. This sample, therefore, represents an important milestone of the project – the moment we confirmed that our method truly works. The image captures the lyophilization step, during which the peptide is being dried after conjugation. The visible transition from yellow to red color is related to changes in the fluorophore’s environment as the sample dries." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Banka_s_fluorescencne_znacenym_glutathionem_242a93510b.jpg 117w," sizes="100vw" width="1600" height="2133">The innovative labeling method developed at IOCB Prague offers a universal, precise, gentle, and permanent way of attaching fluorescent dyes – suitable for small molecules as well as large peptides and proteins. These highly stable labels allow scientists to monitor the movement of selected biomolecules in cells and study their interactions with their environment. The technology expands the possibilities for research in biology, chemistry, and materials science and may contribute to the development of new diagnostic and therapeutic tools.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Angew_Chem_Int_Ed_Engl_2025_Nov_2e15338_figure_1_36885ae230.jpg" alt="Angew Chem Int Ed Engl. 2025 Nov 2:e15338: Figure 1 - Comparison of different labeling methods: a) iodoacetamide, b) maleimide, and c) MTS and our approach." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Angew_Chem_Int_Ed_Engl_2025_Nov_2e15338_figure_1_36885ae230.jpg 245w," sizes="100vw" width="1344" height="375"><blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://onlinelibrary.wiley.com/doi/10.1002/anie.202515338">Thiosulfonate-Derived BODIPY “Stick and Glue” Strategy for Fluorescent Thiol Labeling</a></h4>Lucie Šálková, David Dunlop, B. Buse Tütüncü, Dr. Zdeněk Knejzlík, Dr. Tomáš SlaninaAngew Chem Int Ed Engl. 2025 Nov 2:e15338.&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202515338">https://doi.org/10.1002/anie.202515338</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>

Stick and Glue! Tomáš Slanina and his team introduce a new biomolecule-labeling method for more precise observation of cellular processes

Dive into the world of sample preparation with industry expert Wade Hasenour as he shares invaluable insights on rotary evaporation, centrifugal evaporation, and freeze drying. Learn about the latest advancements, challenges, and future trends in sample prep techniques for chromatography. Discover how to choose the right method for your needs, optimize your workflow, and leverage automation for better results. Whether you're a seasoned chromatographer or just starting out, this video offers essential knowledge to enhance your sample preparation skills and achieve more reliable outcomes in your research.<h3>Video Transcription</h3>Interviewer: Hi Wade, I was hoping you could briefly introduce yourself and tell us about your current role.Wade: Yeah, thanks for having me on here. I currently serve at Genevac as a Regional Sales Manager, and I cover the Midwest, South, and Southeast regions.My career began with a strong foundation in biochemistry. I worked in quality assurance in a lab, then moved into pharmacokinetics and worked in that kind of lab environment as well. After that, I transitioned into a sales role working for Shimadzu, where I specialized in chromatography systems. Later I joined Büchi, where I managed the entire product portfolio. And now I’m at Genevac, where I focus mainly on centrifugal evaporation.Interviewer: As you know, we spend a lot of time here talking about chromatography and mass spectrometry. Today I was hoping we could talk about sample preparation. I’m curious: what are some of the most significant changes or advancements you’ve observed in sample preparation throughout your career?Wade: In sample preparation, the biggest transformation I’ve seen is around robotics and automation. There’s a much stronger focus on it now. Companies are hiring consultants to look at workflows and ask:<ul><li>Where can we improve this?</li><li>How do we make it easier?</li><li>How do we reduce time in production, whether that’s for an API, an oligonucleotide, whatever it may be?</li></ul>The goal is to improve throughput and profitability.While chemists focus on the quality of their work, upper management is pushing for automation and more hands-off approaches. That reduces liability, lowers the chance of people being exposed to hazardous chemicals, and of course, “time is money” for commercial labs and many of our customers.Interviewer: That makes all the sense in the world. I was hoping we could get into sample preparation techniques in general. I’m very familiar with nitrogen blowdown because that’s what Organomation specializes in, but could you walk us through some basic principles of rotary evaporation, centrifugal evaporation, and freeze-drying?Wade: Sure. As a basis, the first thing you need to understand—whether it’s rotary evaporation, centrifugal evaporation, or freeze-drying—is that everything is centered around pressure control.When you reduce the pressure, it becomes easier to evaporate solvents in a more gentle way. All three techniques rely on that, but differ in how much you reduce the pressure and how you apply heat. By controlling pressure, you’re also controlling the effective boiling point and thus the temperature of your sample.With rotary evaporation, the traditional method uses gentle heating via a water bath. Water is a great medium to transfer heat. In terms of efficiency and how well it evaporates, water is an excellent middle ground compared to IR lamps and other heating methods.The rotating flask creates a thin film and large surface area, and the reduced pressure lowers the boiling point so solvents evaporate gently and efficiently. The vapor then goes to a condenser, gets cooled, and ends up in the receiving flask.With centrifugal evaporation, the principle is similar—reduced pressure and heating—but typically the vacuum goes lower. This is especially useful for very temperature-sensitive samples like oligonucleotides, RNA therapeutics, ADCs, peptide linkers, etc.You can, for example, run at around 40 °C at ~8 mbar. You place Falcon tubes or microplates into a rotor and evaporate many samples at once. It’s very high-throughput. Often IR is used for heating, which is gentle but can take longer. The big benefit is that you can process hundreds of samples simultaneously, so per sample, the time can actually be faster than doing them one by one on a rotary evaporator.Both rotary and centrifugal evaporators can dry samples completely and are used at various points in the workflow—synthesis, purification, and formulation.Freeze-drying (lyophilization) is a bit different but still in the same family. You lower the pressure so much that water (or other suitable solvents) goes from solid directly to gas—sublimation—with no liquid phase. This preserves the structure of the sample and is excellent for stability and shelf-life.You can freeze-dry samples in trays, vials, Falcon tubes, flasks, and other formats. The idea is to freeze the sample and then pull off the frozen solvent directly into the gas phase, which is why freezing conditions can strongly influence crystallization and final product characteristics.You often see all three technologies in the same lab: rotary evaporator for synthesis scale-ups or solvent exchange, centrifugal evaporation for high-throughput concentration or dry-down, and freeze-dryers for final formulations and long-term stability.Interviewer: That’s very helpful. It’s always been easy to introduce rotary evaporation as “one sample at a time.” How do you see the transition to centrifugal evaporation in practice?Wade: A common pattern is this: you have a few chemists, each with their own rotary evaporator, doing one or two samples at a time. There’s no serious bottleneck.But once the lab’s throughput increases, they hit that bottleneck—too many samples piling up. That’s when centrifugal evaporation becomes attractive: you can process dozens or hundreds of samples at once.Another factor is solvent behavior. Some harsh or high-boiling solvents, like DMSO, are problematic on a rotary evaporator—they bump easily. Centrifugal evaporators use centrifugal force to help suppress bumping and boiling over, which prevents cross-contamination and sample loss. That centrifugal component is key—extra “gravity” keeps the liquid in place.On a rotary evaporator, high-boiling solvents can be difficult to remove without bumping or risking analyte loss. But in a centrifugal evaporator, you can handle a wide range—from low-boiling to high-boiling solvents—more safely and gently.And that’s really the point: preserving the analyte. When people hear “bumping,” they think “losing sample,” “destroying my yield,” and they’re right to be concerned.Interviewer: You touched on efficiency, sample integrity, and cost-effectiveness. Could you expand on how the different techniques compare in those areas?Wade: Sure. I’ll put on my sales hat here.The first question I have to ask clients—though I try to do it gently—is: What’s your budget and what scale are you working at?<ul><li>If someone says, “I have $5,000,” the realistic option is usually a rotary evaporator.</li><li>Rotary systems can be benchtop or scaled up to industrial sizes.</li><li>Centrifugal evaporators are typically high-throughput lab instruments; you don’t often see warehouse-sized centrifugal systems.</li></ul>So cost-effectiveness is the first filter.Then we talk application:<ul><li>If you’re working with a single low-boiling solvent and just a few samples, rotary evaporation is quick and efficient.</li><li>If you’re working with many samples, mixed solvent systems, or very temperature-sensitive analytes, centrifugal evaporation or freeze-drying might be better.</li></ul>For sample integrity, anything that significantly reduces pressure and allows you to keep temperatures low will help preserve sensitive compounds—proteins, oligos, peptides. That’s where centrifugal evaporation and freeze-drying shine. Rotary evaporation sometimes can’t get the pressure low enough, so bath temperatures or solvent boiling points may end up higher than the sample’s stability limit, and you risk denaturation or degradation.For efficiency, if you only have a few samples, rotary evaporation is hard to beat for speed, especially with water as the heat transfer medium. Genevac also has instruments like the Rocket, which use water to transfer heat and are very efficient for low- to mid-boiling solvents, combining some advantages of both worlds.But if you’ve got 1,000 samples, centrifugal evaporation wins on throughput per sample. The conversation with clients is always:<ul><li>What’s your sample load?</li><li>Which solvents are you using?</li><li>How sensitive is your analyte?</li><li>And what’s your budget?</li></ul>Interviewer: What types of samples or analytes are best suited for each of the main evaporation techniques?Wade: Broadly speaking:<ul><li>Rotary evaporation is ideal for APIs and classic organic synthesis—general solvent removal on flasks, reaction work-up, and straightforward solvent exchanges.</li><li>Centrifugal evaporation is great for mixed solvent systems, temperature-sensitive analytes, and high-throughput workflows: RNA therapeutics, oligonucleotide synthesis, drug screening, diagnostic reagent kits, microplates, etc.</li><li>Freeze-drying is best for biological samples, peptides, and analytes requiring long-term stability or shelf-life. It’s often the final formulation or storage step once purification and concentration are done.</li></ul>Nitrogen blowdown (like Organomation’s systems) fits in as well—especially in workflows where you’re concentrating down to GC vials or small volumes, often after larger-scale evaporation.Interviewer: From a sales perspective, I see many labs still using homemade nitrogen blowdown setups. We’re trying to move them toward dedicated equipment. You must encounter similar situations. Could you share a challenging sample prep scenario and how you dealt with it?Wade: Honestly, the story is often similar: hard solvents and sensitive samples.DMSO is a big one. It doesn’t dry down well on rotary evaporators—it bumps, and people worry about losing sample. We get called in to “save the day” and often customize a solution.Two groups stand out:<ul><li>Oligonucleotides and RNA therapeutics</li><li>Labs working with mixed low- and high-boiling solvents</li></ul>They’re worried about cross-contamination in well plates and maintaining integrity of very sensitive analytes. Competitive technologies sometimes don’t provide enough centrifugal force to keep everything in place, so vapor and droplets can move between wells during evaporation.Our approach with Genevac is to position systems as anti-bumping, gentle evaporators. We add features like inert gas purging, which is useful when people use diethyl ether and other explosive solvents, so you avoid creating hazardous conditions.The real challenge is almost always twofold:<ol><li>Prevent cross-contamination and degradation.</li><li>Improve throughput and consistency compared to existing methods.</li></ol>To solve this, we don’t just impose a “one-size-fits-all” recipe. We ask:<ul><li>What solvents are you using?</li><li>How long does your current process take?</li><li>What problems are you seeing?</li></ul>The hard part isn’t the physics or chemistry—it’s understanding the client’s process and building enough trust so they share details honestly. Once we have that, we often co-develop a configuration or workflow with them. In a few cases, we’ve even created new product options in collaboration with customers.Interviewer: What advice would you give chromatographers who are just starting to explore different sample preparation techniques?Wade: First, something slightly outside sample prep: check solvent miscibility. When I sold chromatography systems, I often saw people using solvent mixtures that would cause problems—locking up LC systems and creating all kinds of issues. Using miscibility charts can save a lot of pain.For sample prep itself:<ul><li>Know your analyte: boiling point, reactivity, and stability.</li><li>Choose your method based on sample sensitivity, solvent volatility, throughput, and budget.</li><li>Don’t hesitate to leverage automation. Consultants and vendors are pushing it for a reason—it reduces manual workload, increases reproducibility, and actually improves job security by letting chemists focus on higher-value work like synthesis and method development.</li></ul>Interviewer: You mentioned consultants. What resources or tools would you recommend for people doing research in this area?Wade: Many instrument companies—Shimadzu, Genevac, Büchi, and others—publish extensive application notes and host webinars. Those are great starting points.There are also:<ul><li>Online learning platforms like Coursera (though not always sample-prep specific)</li><li>Trade magazines and websites like LCGC, which cover chromatography fundamentals and advanced applications</li><li>Professional organizations such as ACS for networking and technical resources</li><li>Classic textbooks on instrumental analysis—many are still only in print, but they’re very solid</li><li>Peer-reviewed articles and instrumentation forums</li></ul>And just as important: talk to other scientists. Many chemists are quite private and hesitate to reach out, but networking can be extremely valuable.Interviewer: We’ve talked a lot about automation already, but how do you see sample prep techniques evolving in the coming years?Wade: We’ll see more compact systems, but the big changes will be AI and automation playing a bigger role across the entire workflow.I’ve already seen robots:<ul><li>Take samples off an RNA synthesizer</li><li>Transfer them to an evaporation station</li><li>Move dried samples into the next instrument</li></ul>There are many micro-steps—cleaning glassware, labeling, reconstitution—where robots can help. Even eliminating one of those bottlenecks can improve throughput significantly.Automation and AI will help:<ul><li>Streamline workflows</li><li>Reduce human error (“person sitting in a chair” error, as I like to joke)</li><li>Maintain compliance and traceability</li><li>Let scientists focus on analysis and interpretation rather than repetitive manual tasks</li></ul>Interviewer: In closing, is there anything else you’d like to share about the importance of sample prep in chromatography?Wade: I’d say: give sample prep the thought it deserves. Choosing the right technique doesn’t just help you reach your goals faster; it also opens up possibilities for more complex and innovative analyses.Sample preparation is the foundation for reliable results. Ensuring consistency and optimizing your workflow are key to success in chromatography and analytical science in general.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Sample Preparation Mastery: Expert Insights on Evaporation Techniques for Chromatography

Article

Product

Video

Scientists

Scientific article

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

KNAUER Eurokat Na Ion Exclusion/Ligand Exchange Columns

The ionic form Na is best suited for the analysis of sugars oligomers up to a degree of polymerization (DP) of 8. 

Analysis of sugar oligomersThe ionic form Na is best suited for the analysis of sugars oligomers up to a degree of polymerization (DP) of 8. It is a cation exchange material with 6 % cross linkage of the styrenedivinylbenzene copolymer. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.&nbsp;<h5>Properties</h5><ul><li>Polymer phase for the determination of sugar oligomers</li><li>Na form, 6 % cross linkage</li><li>Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5>Technical Data</h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Na, available in 10 μm particle size, pressure stable up to maximum 100 bar</li></ul><h5>Recommended application areas</h5><ul><li>Especially designed for the analysis of sugar oligomers, separates carbohydrates up to DP 8</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp" alt="KNAUER: Eurokat Na Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp 188w," sizes="100vw" width="686" height="568"><blockquote>Tip: Eurokat columns are typically used with 100 % aqueous eluents. For storage, please avoid microbial growth by flushing the Eurokat column with 0.45 µm filtered ultrapure water and storing it at 4 °C.</blockquote><h3>KNAUER Ion Exclusion and Ligand Exchange Columns</h3>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer<ul><li>Modifications<ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li>High Temperature Methods: Up to 90 °C</li><li>Method Development: Optional up to 10 % organic solvent</li><li>Chemically stable: pH 1–12</li><li>Sustainability: Aqueous eluents protect the environment and are cost-effectiv</li><li>User Friendly: Simple and robust methods for daily analysis. Versatile in its use.</li><li>Simplicity: Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4>Characteristics&nbsp;</h4>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.<h4>Choice of chromatographic conditions&nbsp;</h4>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;<h4>Stability&nbsp;</h4>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;<h4>Application areas&nbsp;</h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570">

Galochrom Chiral ION-QD HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QD-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</blockquote><h4>Preparative Columns</h4><ul><li>Dimensions [mm]: 150 x 20<ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION05-P150</li><li>Price [€]: 9 800</li></ul></li><li>Dimensions [mm]: 250 x 20<ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION06-P250</li><li>Price [€]: 11 300</li></ul></li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241">ION-QN</a> and ION-QD unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QN-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</blockquote><h4>Preparative Columns</h4><ul><li>Dimensions [mm]: 150 x 20<ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION03-P150</li><li>Price [€]: 9 800</li></ul></li><li>Dimensions [mm]: 250 x 20<ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION04-P250</li><li>Price [€]: 11 300</li></ul></li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral ION-QN and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240">ION-QD</a> unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Thermo Scientific Hypersil GOLD SAX HPLC Columns

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil GOLD™ SAX LC Columns.

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil GOLD™ SAX LC Columns. These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. Based on high purity silica bonded with an ion-exchange ligand, Hypersil GOLD SAX LC Columns provide outstanding peak shape and sensitivity.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Column type: Ion Exchange</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Silica</li><li>Stationary Phase: Quaternary Amine</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L14</li><li>Carbon Load: 2.5%</li><li>Phase: Ion Exchange</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Acclaim Trinity P1 HPLC Columns

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific Acclaim Trinity P1 LC Columns.

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity.The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance.<h5>Superior Performance for Pharmaceutical Applications</h5><ul><li>Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes)</li><li>Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents</li><li>Distinctive spatial separation of the anion-exchange and cation-exchange regions</li><li>Flexible method development with each retention mechanism controlled independently</li><li>Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD)</li><li>Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths</li></ul>Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.<h4>Specifications</h4><ul><li>For use with: ELSD, UV Detector</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Nanopolymer Hybrid Silica</li><li>Stationary phase: Reversed Phase/Anion Exchange/Cation Exchange</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: Reversed Phase and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Trinity P2 HPLC Columns

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific Acclaim Trinity P2 LC Columns.

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC.The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions.<h5>High Selectivity for Counterion Analysis</h5><ul><li>Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD)</li><li>Hydrolytically stable</li><li>High efficiency</li><li>Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Spherical, Fully Porous Silica</li><li>Stationary phase: Spherical, Nanopolymer Hybrid Silica</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: HILIC and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Mixed Mode WAX-1 HPLC Columns

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific Acclaim Mixed-Mode WAX-1 LC columns. 

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.<h5>Ideal Selectivity for Separating Anionic Molecules</h5><ul><li>Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes</li><li>Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids</li><li>Selectivity orthogonal to reversed-phase columns</li><li>Excellent column efficiency and peak symmetry</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Hydrophobic Alkyl Chain with an Ionizable Terminus</li><li>USP Type: L78</li><li>Surface Area: 300 m²/g</li><li>Temperature: 50°C</li><li>Phase: Reversed Phase, Ion Exchange, HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Mixed Mode WCX-1 HPLC Columns

Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific Acclaim Mixed-Mode WCX-1 LC columns.

Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific™ Acclaim™ Mixed-Mode WCX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with a carboxyl terminus that provides both hydrophobic reversed-phase and weak cation-exchange properties. This multimode separation mechanism delivers simultaneous separation of basic and neutral compounds. By independently or simultaneously tuning the mobile phase ionic strength, pH, and organic modifier, you can control the selectivity of ionizable and neutral compounds.<h5>Retains and Separates Highly Hydrophilic to Highly Hydrophobic Basic Molecules</h5><ul><li>Multimode retention mechanism: reversed-phase, weak cation-exchange, anion-exclusion, and HILIC modes</li><li>Selectivity complementary to C18 reversed-phase columns</li><li>Efficient primary column for a variety of basic analytes; also an ideal column for secondary methods development</li><li>Versatile in applications that require different selectivities</li><li>Symmetrical peak shapes and excellent efficiency</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>For use with: RPLC, Cation Exchange, Ion-Exclusion, HILIC</li><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Silica</li><li>Stationary phase: Hydrophobic Alkyl Chain with a Carboxyl Terminus</li><li>USP Type: L85</li><li>Surface Area: 300 m²/g</li><li>Temperature: 50°C</li><li>Phase: Reversed Phase, Ion Exchange, Ion Exclusion, HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Phenomenex Rezex RCM-Monosaccharide Ca+2 (8%) IEX HPLC Columns

Rezex RCM-Monosaccharide Ca²⁺ (8%) HPLC column (300 × 7.8 mm) enables separation of monosaccharides, sugar alcohols, and saccharides with easy regeneration and USP L19 compliance.

<h4>Key Features of the Rezex RCM-Monosaccharide Ca+2 (8%) Ion Exclusion HPLC Column:</h4><ul><li>8% cross-linked calcium</li><li>For use with monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars</li><li>Class separation of di-, tri-, and tetra- saccharides</li><li>Easy regeneration with calcium nitrate solutions</li><li>USP: L19</li></ul><h4>Specifications and Operating Recommendations</h4><ul><li>Part No.: 00H-0130-K0</li><li>Ionic Form: Calcium</li><li>Standard Dimensions: 300 x 7.8 mm</li><li>Matrix: Sulfonated Styrene Divinyl Benzene</li><li>Cross Linking: 8 %</li><li>Particle Size (μm): 8</li><li>Min. Efficiency (p/m) based on last peak: 35,000</li><li>Typical Pressure (psi @ Testing Flow Rate): 260</li><li>Max. Pressure (psi @ Max Flow Rate): 1,000</li><li>Max. Flow Rate (mL/min): 1.0</li><li>Max. Temperature (°C): 85</li><li>Typical Mobile Phase: Water</li><li>pH Range: Neutral</li><li>Guard Column Part: 03B-0130-K0</li></ul><h4>Cross Referencing Guide</h4><ul><li>Bio-Rad®Aminex®: HPX-87C (125-0095)</li><li>Supelco®SUPELCOGEL™: SUPELCOGEL (Ca)</li><li>Waters®Sugar-Pak™: Sugar-Pak 1</li><li>Transgenomic®CARBOSep™: CARBOSep (CHO-820)</li><li>Sepax®Carbomix®: Carbomix (Ca)</li></ul>

Watrex Polymer IEX HPLC columns

Do you need HPLC columns for the determination of organic acids, alcohols and sugars? Columns filled with a monodisperse sorbent based on sulfonated styrenedivinylbenzene.

<a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=watrex+polymer+iex&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex Polymer IEX™</a> is the Europe’s leading sorbent for ion exclusion analysis of alcohols, carboxylic acids, sugars, and polysacharides<ul><li>H⁺, Ca²⁺, Pb²⁺ and Na⁺ forms</li><li>Unique monodispersed sorbent based on sulfonated polystyrene</li><li>Bulk packing materials available</li></ul><h3>H form</h3>The hydrogen form is suitable for organic acids, alcohols and some sugars in natural materials. The column is particularly suitable for the analysis of fruit drinks, wine, etc. Its great advantage is the high stability of retention times and the wide concentration range of analytes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Column_250x8mm_IEX_H_4da682fa13.jpg" alt="Watrex: Column 250x8mm IEX H"><h3>Ca form</h3>The calcium form is characterized by good selectivity for the separation of mono- and disaccharides in natural materials. The column must be protected from anions forming insoluble calcium salts (phosphates, sulphates, etc.) and cations with a higher affinity for the sorbent than calcium ions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Column_250x8mm_IEX_Ca_b840dddcce.jpg" alt="Watrex: Column 250x8mm IEX Ca"><h3>Pb form</h3>The lead form is suitable for the separation of mono- and disaccharides in natural materials. The column must be protected from anions forming insoluble lead salts (phosphates, sulphates, etc.) and cations with a higherUse the appropriate pre-column to extend the life of the analytical column.<h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>WI020223 Watrex 300x8 mm Polymer IEX H form, 8 um</li><li>WI020213 Watrex 300x8 mm Polymer IEX Ca form, 8 um</li><li>WI020253 Watrex 300x8 mm Polymer IEX Pb form, 8 um</li><li>WI02022 Watrex 250x8 mm Polymer IEX H form, 8 um (Price 926 € ex VAT)</li><li>WI02021 Watrex 250x8 mm Polymer IEX Ca form, 8 um (Price 926 € ex VAT)</li><li>WI02025 Watrex 250x8 mm Polymer IEX Pb form, 8 um (Price 926 € ex VAT)</li><li>WI02122 Guard column insert 10x4.0 mm PEEK Polymer IEX H 8 um, 10 pcs</li><li>WI02121 Guard column insert 10x4.0 mm PEEK Polymer IEX Ca 8 um, 10 pcs</li><li>WI029012 Guard column holder (for 10x4.0 mm PEEK insert)</li></ul>

Products from category IEX HPLC columns - page 1

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