Thermo Scientific Acclaim Mixed Mode WAX-1 HPLC Columns

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.

Ideal Selectivity for Separating Anionic Molecules

• Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes
• Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids
• Selectivity orthogonal to reversed-phase columns
• Excellent column efficiency and peak symmetry
• Stable between pH 2.5 and 7.5
• Available in 3 and 5μm particle sizes with an average pore diameter of 120Å

Specifications

• Column type: Mixed mode
• Max. Pressure: 5800 psi (400 bar)
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Stationary phase: Hydrophobic Alkyl Chain with an Ionizable Terminus
• USP Type: L78
• Surface Area: 300 m²/g
• Temperature: 50°C
• Phase: Reversed Phase, Ion Exchange, HILIC
• Pore Size: 120 Å
• pH: 2.5 to 7.5

About the Acclaim Family of HPLC Columns

Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.

• Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs
• Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations

Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns