Waters SEC Columns

Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

SEC is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.

The pore size and volume of the SEC particles used in the packed column drives this isocratic based technique, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.

Overview

• Gain efficiency with this “platform based” method for proteins ranging from 10,000 to 650,000 Daltons, leveraging MaxPeak Premier Technology coupled with BEH-PEO Particle Technology    
• Minimize non-desired secondary interactions for improved separation reproducibility and results with Ethylene Bridged Hybrid (BEH) SEC particles
• Ensure reliable column-to-column and batch-to-batch performance with columns quality control tested with relevant peptide, protein, and mAb samples  
• Achieve reliable high throughput analysis on UPLC systems with our 1.7 µm particle size offering and reproducible separations on any HPLC, UHPLC, or UPLC system to address different application needs with 2.5 µm 
• Extend analytical column life and lower cost per analyses with SEC Guards 
• Confirm on-site SEC column and LC system performance with available BEH125, BEH200, BEH450, and mAb Size Variant Standards   
• Manufactured in a cGMP, ISO 9001 certified plant using stringent manufacturing protocols and ultra-pure reagents

Recommended Use: For size-based peptide, protein, and viral vector analyses.

System Dispersion Specifications for Column Recommendations

LC System Dispersions <20 µL (UPLC)

• ACQUITY Premier Protein SEC 250 Å Guards and Columns
• ACQUITY UPLC Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns
• 1.7 µm guards and columns for low dispersion UPLC systems

LC System Dispersions >20 µL – ≤35 µL (UHPLC)

• XBridge Premier Protein SEC 250 Å Guards and Columns
• XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns
• BioResolve mAb SEC Guards and Columns
• 2.5 µm guards and columns for UHPLC systems

LC System Dispersions >35 µL (HPLC)

• XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns
• XBridge Premier Protein SEC 250 Å Guards and Columns
• BioResolve mAb SEC Guards and Columns
• BioSuite SEC Guards and Columns
• 3.5 µm guards and columns for HPLC systems

GTxResolve SEC Columns

GTxResolve Premier BEH SEC 450 Å Columns

• 2.5 µm particle size columns for UHPLC and UPLC separations
• Lower secondary interactions and lower noise suitable for platform methods
• Low adsorption hardware necessary for analysis of biologics samples
• 450 Å pore size with up to 50% higher resolving power reducing need for sample consumption, resulting in faster analyses
  • Explore GTxResolve SEC Columns

GTxResolve Premier SEC 1000 Å Columns

• 3 µm columns for UHPLC and UPLC separations
• 1000 Å columns with high efficiency for aggregate analysis of GTx drugs with diameter of 80-200 nm
• Low secondary interaction & improved compatibility with universally applied detector, such as MALS
• Low adsorption hardware necessary for analysis of biologics samples
  • Explore GTxResolve SEC Columns

Waters: GTxResolve SEC Columns

Protein SEC Columns

ACQUITY Premier Protein SEC 250 Å Guards and Columns

• Sub-2-μm particle columns for UPLC separations
• 250 Å pore size
• High throughput & resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems
• Available SEC Guard Columns to extend analytical SEC Column life
• Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology
  • Explore ACQUITY Premier Protein BEH SEC Guards and Columns

Waters: ACQUITY Premier Columns featuring MaxPeak HPS Technology

XBridge Premier Protein SEC 250 Å Guards and Columns

• 2.5 μm particle columns for HPLC and UHPLC separations
• 250 Å pore size
• High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system
• Available SEC Guard Columns to extend analytical SEC Column life
• Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology
• Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency
  • Explore XBridge Premier Protein BEH SEC Columns

Waters: XBridge Premier Columns

BioResolve mAb SEC Guards and Columns

• 2.5 μm particle columns for UPLC, UHPLC, and HPLC separations
• 200 Å pore size
• Obtain reproducible, accurate quantitation of monoclonal antibodies (mAbs), associated high molecular weight aggregates (>300,000 Da), and lower molecular weight fragments (e.g., <100,000 Da)
• Each packed column is tested with NIST-based, mAb size variant standard to help ensure batch-to-batch and column-to-column performance consistency
  • Explore BioResolve mAb SEC Guards and Columns

ACQUITY UPLC Protein BEH SEC Guards and Columns

• Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)
• Pore size options include: 125 Å, 200 Å, and 450 Å
• Available SEC Guard Columns to extend analytical SEC Column life
• Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency
• 10x faster than traditional HPLC-based SEC
  • Explore ACQUITY UPLC Protein BEH SEC Guards and Columns

Waters: Acquity UPLC columns

XBridge Protein BEH SEC Columns

• 2.5 μm particle columns for use on any UPLC, UHPLC, or HPLC system
• Pore size options include: 125 Å, 200 Å, and 450 Å
• Available SEC Guard Columns to extend analytical SEC Column life
• Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency
  • Explore XBridge Protein BEH SEC Columns

Waters: XBridge Columns

Peptide SEC Columns

BioSuite SEC Columns

• Silica-based diol SEC columns
• Molecular weight exclusion limits are determined primarily by the selected pore size
• Available in analytical and semi-preparative column sizes
  • Explore BioSuite SEC Columns

Waters: BioSuite Columns

Protein-Pak SEC Columns

• Based on a 10 μm diol-bonded silica
• Resolve proteins that differ in molecular weight by a factor of two
• Distinguish proteins differing by as little as 15% in molecular weight
  • Explore Protein-Pak SEC Columns

Waters: Protein-Pak Columns

Choose the right SEC Column for your lab

SEC is ideal for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic peptides, proteins, and viral vectors, but choosing the right column for your analysis is important. Use the following criteria to choose a column that provides reliable, high quality SEC analyses of various biomolecules, including insulin, monoclonal antibodies, and adeno associated viruses (AAVs).

What to consider when choosing an SEC Column:

• What is the molecular weight of your sample?
• What is the dispersion of your LC system?
• Are you separating components <2 fold difference in molecular weight (MW)?
• Are you separating components >2 fold difference in MW?

Waters: Choose the right Size-exclusion chromatography (SEC) column for your analysis with Waters SEC columns selection guide.

Addressing the complexity of SEC Separations

Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards.

With MaxPeak Premier SEC Columns, you can:

• Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications
• Use a ‘platform SEC method’ to reduce method development time and cost
• Ensure batch-to-batch and column-to-column performance

Waters: Classes of therapeutic proteins separated on an ACQUITY Premier Protein SEC Column.