<p><strong>🎤Brad Haubrich </strong>(Touro University Nevada )</p><p><strong>Neglected and opportunistic infectious diseases affect some of the world’s most vulnerable populations—but often receive the least attention from traditional drug discovery pipelines.</strong></p><p>In this episode of Concentrating on Chromatography, host David Oliva sits down with Brad Haubrich to explore how early-stage drug discovery is being applied to fungal and parasitic pathogens, including those responsible for neglected tropical diseases and infections that disproportionately affect immunocompromised patients.</p><p>Brad shares how his lab approaches drug discovery when the pathogen is eukaryotic—and therefore biologically similar to humans—making selectivity one of the biggest challenges. The conversation covers:</p><ul><li>What defines neglected and opportunistic diseases—and why commercial incentives often fall short</li><li>Target-based vs. phenotypic drug discovery and when each approach makes sense</li><li>Using binding kinetics and residence time to improve selectivity and reduce off-target effects</li><li>Where chromatography, metabolomics, and mass spectrometry still play a critical role—even when not front-and-center</li><li>The growing (and realistic) role of AI in drug discovery, especially for underfunded disease areas</li><li>Why World Neglected Tropical Diseases Day (January 30) matters for raising awareness and accelerating progress</li></ul><p>This episode highlights how analytical chemistry, separation science, and biological insight intersect in the earliest stages of drug discovery—and why progress in this space depends as much on collaboration and curiosity as it does on technology.</p><h3><strong>Video Transcription</strong></h3><p>In this interview, Dr. Brad Haubrich discusses his research focus on neglected tropical diseases (NTDs) and opportunistic infections. His interest in this field originated during graduate and postdoctoral studies, particularly through work on African sleeping sickness. Neglected diseases are defined as conditions that predominantly affect populations in low- and middle-income countries, where both healthcare infrastructure and commercial incentives for drug development are limited. Similarly, opportunistic infections disproportionately impact immunocompromised individuals, including patients with cancer, transplant recipients, and those undergoing immunosuppressive therapy.</p><p>A key challenge in this area is the limited commercial interest in drug discovery, resulting in fewer therapeutic options despite significant unmet medical needs. The research emphasizes fungal and parasitic pathogens, which present distinct challenges compared to bacterial targets, particularly due to shared biological pathways between pathogens and human hosts.</p><h4><span><strong>Drug Discovery Strategies and Challenges</strong></span></h4><p>The discussion highlights two complementary approaches used in the lab: target-based and phenotypic drug discovery. Target-based strategies involve identifying and biochemically characterizing specific pathogen proteins, while phenotypic approaches assess compound activity directly in living cells. Each approach has advantages and limitations, particularly in identifying molecular targets and ensuring biological relevance.</p><p>A major scientific challenge is achieving selectivity. Because eukaryotic pathogens share many proteins with human cells, identifying compounds that selectively inhibit pathogen targets without affecting host systems is difficult. To address this, the lab incorporates <strong>binding kinetics</strong> as an early screening parameter. Instead of relying solely on traditional potency metrics, this approach evaluates how long a compound interacts with its target, enabling the identification of molecules with prolonged, selective binding (pseudo-irreversible inhibition), which may improve therapeutic specificity.</p><h4><span><strong>Role of Analytical Techniques and Chromatography</strong></span></h4><p>Although chromatography is not central to all workflows in the lab, it remains critical in several areas. It is widely used by collaborators for compound purification and plays an important role in emerging projects focused on <strong>pharmacognosy</strong>, where bioactive compounds are isolated from natural sources such as desert flora.</p><p>Chromatography, coupled with mass spectrometry, is also essential for <strong>metabolomics-based target identification</strong>. In phenotypic screening, where the molecular target is initially unknown, changes in metabolite profiles can provide insight into biochemical pathways affected by candidate compounds, supporting target deconvolution.</p><h4><span><strong>Impact, Training, and Future Directions</strong></span></h4><p>The success of research in neglected diseases is not solely measured by commercial outcomes but also by contributions to scientific knowledge and global health. Even incremental findings can support future therapeutic breakthroughs.</p><p>The interview also emphasizes the role of students, particularly in academic medical settings, where participation in early-stage drug discovery provides valuable insight into the full pipeline from molecular discovery to clinical application.</p><p>Looking ahead, <strong>artificial intelligence (AI)</strong> is identified as a key driver of innovation in drug discovery. AI tools can accelerate compound screening, predict bioactivity, and even suggest synthetic pathways. However, their effectiveness in neglected disease research is currently limited by the availability of large, high-quality datasets.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Drug Discovery Where the Market Doesn’t Go: Targeting Neglected & Opportunistic Pathogens

<p><strong>This study investigates the optimization and functional properties of kombucha enriched with </strong><i><strong>Prunus spinosa</strong></i><strong> juice and bee products (propolis, pollen, and bee bread). Advanced analytical techniques (HPLC–DAD, LC–MS/MS) revealed enhanced phenolic, amino acid, and antioxidant profiles, with bee bread enrichment showing the highest overall bioactive content and sensory acceptance.</strong></p><p>Propolis-enriched kombucha exhibited the strongest in vitro antidiabetic activity, while pollen enrichment provided the highest amino acid levels. Despite partial losses during digestion, significant retention of bioactives suggests potential postdigestive benefits. These findings highlight the potential of enriched kombucha as a functional beverage with nutraceutical value.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008"><strong>From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility</strong></a></h4><p>Melikenur Türkol, Çiğdem Yıldırım Maviş, and Seydi Yıkmış*</p><p>ACS Omega 2026, 11, 8, 14048–14063</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00008">https://doi.org/10.1021/acsomega.6c00008</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Kombucha tea, a traditionally fermented beverage, is believed to have originated as early as 200–300 BC. In recent decades, it has attracted growing global interest due to its recognized health-promoting properties and its deep cultural roots. <a href="javascript:void(0);">(1,2)</a> Sweetened tea derived from <i>Camellia sinensis</i> is transformed into kombucha through fermentation carried out by a SCOBY (Symbiotic Culture of Bacteria and Yeast), a mixed culture of yeasts and bacteria that forms a cellulose-rich biofilm throughout the process. <a href="javascript:void(0);">(3)</a> SCOBY represents a symbiotic consortium of microorganisms that comprises various yeast genera, including <i>Saccharomyces</i>, <i>Schizosaccharomyces</i>, <i>Zygosaccharomyces</i>, <i>Brettanomyces/Dekkera</i>, and <i>Pichia</i>, as well as both acetic and lactic acid bacteria. <a href="javascript:void(0);">(2)</a> During fermentation, the sugars formed contribute a mild sweetness to the tea, whereas the organic acids synthesized by acetic acid bacteria are responsible for its distinctive sour flavor. <a href="javascript:void(0);">(4)</a> Kombucha tea is traditionally brewed using black tea; however, alternative versions can also be produced with other types of tea. <a href="javascript:void(0);">(5)</a> It has been reported that herbal infusions and fruit juices can also be used for fermentation with the kombucha culture, and due to their chemical composition, these substrates have been shown to serve as promising alternatives. <a href="javascript:void(0);">(6)</a> The antioxidant potential of kombucha tea is primarily linked to its abundant phenolics, with flavonoids playing a significant contributory role. <a href="javascript:void(0);">(7)</a> A high anthocyanin content tends to enhance the antioxidant capacity. <a href="javascript:void(0);">(8)</a> The antioxidant activity of kombucha tea can be improved by incorporating anthocyanin-rich fruits. Accordingly, the use of wild fruits with high anthocyanin content─such as <i>Prunus spinosa</i> L. (blackthorn), <i>Prunus laurocerasus</i> (cherry laurel), and <i>Rubus idaeus</i> (red raspberry)─has been considered a practical way to improve the beverage’s nutritional, functional and sensorial properties. <a href="javascript:void(0);">(9)</a></p><p>Propolis is a naturally occurring material that honeybees gather from the resins of different trees and the buds of various plants. <a href="javascript:void(0);">(28,29)</a> Propolis is a chemically complex matrix comprising more than 300 identified components, including flavonoids, phenolic acids, and their ester derivatives, and is associated with a wide range of biological activities such as antiviral, antioxidant, antimicrobial, anti-inflammatory, antitumor, hepatoprotective, and immunomodulatory effects. <a href="javascript:void(0);">(30−34)</a> Minerals and vitamins are also present in high concentrations in propolis. <a href="javascript:void(0);">(28)</a> Bee pollen is formed when bees agglutinate pollen grains using their saliva, nectar, or honey and store them in granular form in their honeycomb cells. The key role of bee pollen is to ensure the colony’s survival by providing the necessary nutrients, thereby supporting development and maintenance processes. <a href="javascript:void(0);">(35)</a> Bee pollen is a natural product rich in bioactive compounds. More than 250 bioactive compounds have been identified in bee pollen derived from different plant species. These basic chemical components include lipids, amino acids, proteins, carbohydrates, fatty acids, phenolic compounds, carotenoids, flavonoids, vitamins, and bioelements. <a href="javascript:void(0);">(36)</a> Exhibits antihyperglycemic, anti-inflammatory, hepatoprotective, nephroprotective, and anticancer properties thanks to its rich and versatile component mixture. <a href="javascript:void(0);">(37,38)</a> Many of these have demonstrated antioxidant, antimicrobial, antidiabetic. They have shown promising results in the treatment of diabetes and obesity, and their potential as natural therapeutic agents has been evaluated. They have been found to increase insulin sensitivity, reduce oxidative stress, regulate appetite, adjust obesity-related hormone levels, and strengthen antioxidant defense systems. <a href="javascript:void(0);">(39,40)</a> The antimicrobial effect of bee bread has been extensively studied. Bakour et al. found that different bacterial species and fungi are sensitive to the hydromethanolic bee bread extract. <a href="javascript:void(0);">(41)</a> A vital bee byproduct, bee bread is formed after the pollen gathered by bees is enriched with nectar and bee saliva, leading to an ensuing lactic acid fermentation process inside the hives. <a href="javascript:void(0);">(42)</a> Bee bread is high in nutrition, with significant carbohydrates, proteins and lipids. It is a rich source of vitamins, phenolic compounds, minerals and essential amino acids, and exhibits multiple chemical activities. <a href="javascript:void(0);">(43,44)</a></p><p>The Response Surface Method (RSM) is one of the statistical optimization approaches. This method uses empirical modeling techniques to identify relationships between independent variables and the system response using experimental data. It also allows for determining optimal conditions for achieving the desired outcome by evaluating the effects of process variables on the system response. <a href="javascript:void(0);">(45−47)</a></p><p>Previous studies have not extensively investigated the sensory parameters (color, taste, smell, and general acceptance) of kombucha tea enriched with <i>P. spinosa</i> L. and bee products using RSM. The primary aim of this study was to optimize the sensory properties of kombucha formulations enriched with <i>P. spinosa</i> L. and bee-derived ingredients (bee bread, propolis, and pollen) using RSM to determine the most acceptable formulation. While many studies have examined kombucha enriched with various fruit and plant infusions, none have specifically investigated the impact of <i>P. spinosa</i> L. and bee products on kombucha’s sensory and bioactive properties. The study also evaluated the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH•), total anthocyanin content (TAC), phenolic profile (HPLC), microbiological quality, and levels of organic acids amino acids and in vitro bioaccessibility in the developed samples, as well as their antidiabetic activity. RSM-based optimization offers a new methodological approach. It is expected to yield valuable data. This data will improve the functional and disease-fighting properties of kombucha.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>2.9. Analysis of Phenolic Compounds (HPLC)</strong></h5><p>Chromatographic separation was carried out on an ACE Genix C18 column (250 × 4.6 mm, 5 μm; Agilent), following the procedure reported by Portu et al. (2017). <a href="javascript:void(0);">(56)</a> Phenolics were characterized using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent 1260 HPLC</strong></a>–DAD system operated under gradient conditions at 30 °C with a flow rate of 0.80 mL/min. The mobile phase comprised solvent A (water supplemented with 0.1% phosphoric acid) and solvent B, applied according to a predefined gradient program. Samples were injected at a volume of 10 μL, and chromatograms were recorded at 280, 320, and 360 nm. Quantitative results were reported as μg/mL.</p><h5><strong>2.10. Analysis of Organic Acids (HPLC)</strong></h5><p>HPLC was used to determine organic acid profiles, following a method adapted with minor modifications from Coelho et al. <a href="javascript:void(0);">(57)</a> Organic acids were analyzed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent 1260 Infinity LC HPLC system</strong></a> fitted with refractive index (RID) and diode array (DAD) detectors. Samples were filtered through a 0.45 μm syringe filter, and a 20 μL aliquot was injected into an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent Hi-Plex H ion-exchange column</strong></a> (300 × 7.7 mm). Chromatographic separation was carried out using 10.0 mM H<sub>2</sub>SO<sub>4</sub> as the mobile phase at a flow rate of 0.6 mL/min, with the column temperature set to 65 °C and the RID cell maintained at 35 °C. The total run time was 20 min. DAD additionally monitored tartaric, pyruvic, and acetic acids at 210 nm. Quantitative determination was carried out using external calibration standards, and the results were reported in g/L.</p><h5><strong>2.11. Determination of Free Amino Acids (LC–MS)</strong></h5><p>Amino acid composition was determined using a method adapted with minor modifications from the procedure described by Bilgin et al. (2019). <a href="javascript:void(0);">(58)</a> A gradient elution scheme involving mobile phases A and B was applied to obtain chromatographic separation at a flow rate of 0.7 mL/min, yielding a total run duration of 7.5 min. Tandem mass spectrometric analyses were performed on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10"><strong>Agilent 6460 triple quadrupole LC–MS instrument</strong></a> (Agilent Technologies, Waldbronn, Germany) fitted with an electrospray ionization (ESI) interface. The mass spectrometer was operated with a gas temperature of 150 °C, a gas flow rate of 10 L/min, a nebulizer pressure of 40 psi, and a capillary voltage of +2000 V. All analyses were carried out in triplicate, and the results were reported as mg/100 mL.</p><h4><strong>3. Results and Discussion</strong></h4><h5><strong>3.4. Phenolic Compounds (HPLC)</strong></h5><p>As presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008#tbl1">Table 1</a>, the total quantified phenolic content differed significantly among the treatments (<i>p</i> &lt; 0.05). The control sample (BKT) exhibited the lowest phenolic concentration (106.35 ± 2.25 μg/mL), whereas pollen supplementation (PL-BKT) resulted in a substantial increase, reaching 159.23 ± 4.82 μg/mL. Further enhancement of the phenolic pool was achieved through propolis (PB-BKT: 178.66 ± 5.04 μg/mL) and bee bread (BB-BKT: 186.15 ± 0.97 μg/mL) supplementation. No statistically significant difference was observed between PB-BKT and BB-BKT in total quantified phenolics; however, both treatments exhibited significantly higher values than PL-BKT and the control, indicating a more pronounced phenolic enrichment.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_8_14048_14063_Table_1_Changes_in_Total_Phenolic_Compounds_of_Kombucha_Samples_Subjected_to_BKT_PB_BKT_PL_BKT_and_BB_BKT_5a50c7851f.jpg" alt="ACS Omega 2026, 11, 8, 14048–14063: Table 1. Changes in Total Phenolic Compounds of Kombucha Samples Subjected to BKT, PB-BKT, PL-BKT and BB-BKT. Data are presented as mean ± standard deviation (n = 3). Values denoted by different letters within the same row indicate statistically significant differences (p &lt; 0.05). BKT: Blackthorn kombucha tea; PB-BKT: Propolis blackthorn kombucha tea; PL-BKT: Pollen blackthorn kombucha tea; BB-BKT: Bee bread blackthorn kombucha tea. n.d.: could not be detected." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_8_14048_14063_Table_1_Changes_in_Total_Phenolic_Compounds_of_Kombucha_Samples_Subjected_to_BKT_PB_BKT_PL_BKT_and_BB_BKT_5a50c7851f.jpg 159w," sizes="100vw" width="1054" height="1034"></p><p>The compound-level distribution suggests that the observed elevation in total phenolics is driven by selective, matrix-dependent enrichment. Bee bread is a functional food thanks to its bioactive compounds and chemical diversity. <a href="javascript:void(0);">(72)</a> The marked increase in chlorogenic acid concentration in BB-BKT, to 79.04 ± 4.66 μg/mL, was a key contributor to the elevated total phenolic content in this group. In contrast, chlorogenic acid remained undetectable or present at negligible levels in the other treatments. Likewise, the enrichment of thermosonicated kiwi juice with bee bread resulted in a statistically significant elevation in chlorogenic acid levels. <a href="javascript:void(0);">(68)</a> The highest catechin hydrate concentration was observed in PL-BKT (121.2 ± 3.36 μg/mL), indicating that pollen supplementation preferentially enhances the flavan-3-ol fraction. In contrast, the markedly elevated levels of quercetin (47.32 ± 2.07 μg/mL) and naringenin (17.59 ± 0.38 μg/mL) detected in PB-BKT support the flavonoid-enriching effect of propolis (<i>p</i> &lt; 0.05). In another study, propolis supplementation of thermosonicated yellow cherry juice was found to increase quercetin content significantly. Additionally, the exclusive emergence of chrysin in the enriched samples, particularly its pronounced accumulation in BB-BKT (66.42 ± 1.84 μg/mL), suggests that bee bread supplementation can substantially reshape the specific phenolic signature of the matrix. This interpretation is further supported by the highest rosmarinic acid level observed in BB-BKT (3.05 ± 0.24 μg/mL). Conversely, the absence of caffeic acid and the flavones fraction across all treatments (n.d.) indicates that the applied interventions exerted limited effects on specific phenolic subclasses.</p><h5><strong>3.5. Free Amino Acids (LC–MS)</strong></h5><p>The PL-BKT sample, which was supplemented with pollen, exhibited the highest free amino acid content (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008#tbl2">Table 2</a>). This outcome differed significantly from all other groups (<i>p</i> &lt; 0.05). Significant increases were observed in proline (47.37 mg/100 mL), leucine (4.10 mg/100 mL), and lysine (4.54 mg/100 mL), indicating that pollen, due to its high protein content, increases amino acid release during fermentation. In contrast, amino acid levels were lower in the sample supplemented with propolis alone (PB-BKT), and some amino acids (e.g., glutamic acid, histidine, threonine) were not statistically different from the BKT sample (<i>p</i> &gt; 0.05).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_8_14048_14063_Table_2_Effects_of_BKT_PB_BKT_PL_BKT_and_BB_BKT_on_Free_Amino_Acids_mg_100_m_L_f377c7c7e0.jpg" alt="ACS Omega 2026, 11, 8, 14048–14063: Table 2. Effects of BKT, PB-BKT, PL-BKT and BB-BKT on Free Amino Acids (mg/100 mL). The mean value, along with the standard deviation, is given for the data (n = 3). Values indicated by different letters within the same row differ significantly (p &lt; 0.05). BKT: Blackthorn kombucha tea; PB-BKT: Propolis blackthorn kombucha tea; PL-BKT: Pollen blackthorn kombucha tea; BB-BKT: Bee bread blackthorn kombucha tea." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_8_14048_14063_Table_2_Effects_of_BKT_PB_BKT_PL_BKT_and_BB_BKT_on_Free_Amino_Acids_mg_100_m_L_f377c7c7e0.jpg 159w," sizes="100vw" width="1055" height="1033"></p><h4><strong>4. Conclusions</strong></h4><p>This study demonstrated that kombucha enriched with <i>P. spinosa</i> L. juice and bee products (propolis, pollen, and bee bread) provides a robust model for functional beverage design through RSM-based optimization and advanced analytical characterization (HPLC–DAD, LC–MS/MS). From a sensory perspective, the highest overall acceptability was observed in the bee bread-enriched sample (8.32), which also exhibited the strongest antioxidant response (TPC 257.10 mg GAE/100 mL; TFC 36.16 mg CE/100 mL; DPPH• 57.05 μmol TE/mL). HPLC–DAD data revealed that total phenolics increased to 159.23–186.15 μg/mL following enrichment, and that marker compounds, such as chlorogenic acid and catechin derivatives, varied depending on the formulation. LC–MS/MS supported an increase in free amino acids (particularly proline) with pollen supplementation. Propolis addition highlighted the antidiabetic potential, yielding the highest enzyme inhibition (α-glucosidase: 40.7%; α-amylase: 42.02%). Although a partial reduction in bioactives was observed during in vitro digestion, the preservation of phenolic/flavonoid recovery within approximately the 21–30% range indicates that postdigestive activity may be sustained. Future studies are recommended to expand biomarkers using targeted/untargeted LC–HRMS-based metabolomic approaches, to correlate SCOBY microbiota (16S/ITS) with chemical profiles, and to evaluate shelf life and packaging stability (phenolic degradation kinetics, volatile profile), as well as in vivo/preclinical validation (glycemic response, bioaccessibility). A limitation of this work is that batch-specific botanical origin (palynological) identification of the commercial bee products was not performed; therefore, compositional variability due to botanical source cannot be fully excluded.</p></div>

From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility

<p><strong>In the week of April 27, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Separation Science: Analytical Excellence Week 2026: Part 1- Understanding Pharmacopoeia and ACS Compliance</strong></h5><ul><li>Tu, 28.4.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6130">Registration</a></li></ul><p>Discover the critical role of compliant reagents in your lab and learn how to ensure that your analyses meet regulatory standards.</p><h5><strong>2. Agilent Technologies: PFAS &amp; Our Planet: Environmental Monitoring</strong></h5><ul><li>Tu, 28.4.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6140">Registration</a></li></ul><p>Explore evolving PFAS regulations and practical LC-MS/MS workflows for monitoring PFAS in water, soil, and air, including contamination control, sample prep, and reporting strategies.</p><h5><strong>3. Restek Corporation: Air Sampling Media Selection and Analysis</strong></h5><ul><li>Tu, 28.4.2026 14:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6160">Registration</a></li></ul><p>This presentation will give an overview of the selection of air sampling media, covering both whole air and sorbent sampling techniques.</p><h5><strong>4. Tosoh Bioscience: Tosoh Tuesday - What is MALS &amp; When is it Useful?</strong></h5><ul><li>Tu, 28.4.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6145">Registration</a></li></ul><p>Join Tosoh Tuesday to explore MALS fundamentals, practical implementation, and real-world SEC-MALS applications for polymer and biologics characterization.</p><h5><strong>5. Technology Networks: Mass Spectrometry in Drug Discovery: Covalent and Non-Covalent Screening Applications</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6114">Registration</a></li></ul><p>Identifying small-molecule binders for complex biological targets remains a major challenge in early drug discovery.</p><h5><strong>6. Thermo Fisher Scientific: From Sample to Structure: Crosslinking MS Made Practical - Part 1</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6115">Registration</a></li></ul><p>Crosslinking mass spectrometry (XL-MS) is transforming how researchers map protein–protein and protein–nucleic acid interactions.</p><h5><strong>7. SelectScience: Why researchers turn to reversed phase chromatography when purifying GLP-1</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6117">Registration</a></li></ul><p>Purifying synthetic peptides is a critical step in developing advanced biotherapeutics like GLP-1 receptor agonists.</p><h5><strong>8. Phenomenex: Fundamentals First Bio Series – SEC for Biologics</strong></h5><ul><li>We, 29.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6149">Registration</a></li></ul><p>Master SEC fundamentals for biopharma—covering principles, column selection, and troubleshooting with real-world applications in mAbs, AAVs, and nucleic acids.</p><h5><strong>9. SCIEX: Extending the power of LC-MS/MS: advanced workflows with QTRAP®</strong></h5><ul><li>We, 29.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6147">Registration</a></li></ul><p>Discover how QTRAP® workflows enhance LC-MS/MS performance with MS³ selectivity, improving sensitivity, reducing interference, and enabling confident analysis in complex matrices.</p><h5><strong>10. Metrohm: The Basics of Titration explained</strong></h5><ul><li>We, 29.4.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6129">Registration</a></li></ul><p>In this webinar, Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters, will give you an introduction to the basics of titration.</p><h5><strong>11. Separation Science: Analytical Excellence Week 2026: Part 2 - Benefits of PFAS-Tested Consumables</strong></h5><ul><li>Th, 30.4.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6131">Registration</a></li></ul><p>Gain insights into the importance of PFAS-tested additives and solvents for reliable analysis and their impact on the quality of your results.</p><h5><strong>12. Evosep Biosystems: Next-Gen HCP Analysis in Biopharma</strong></h5><ul><li>Th, 30.4.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6151">Registration</a></li></ul><p>Discover how LC-MS is advancing biopharma workflows through scalable, high-throughput proteomics.</p><h5><strong>13. Bruker: Bridging Molecules and Genes: Integrating MALDI Imaging and Spatial Transcriptomics on a Single Tissue Section</strong></h5><ul><li>Th, 30.4.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6132">Registration</a></li></ul><p>Explore how integrating MALDI Imaging with spatial transcriptomics reveals tissue heterogeneity, molecular gradients, and disease-relevant patterns through advanced multimodal workflows.</p><h5><strong>14. Thermo Fisher Scientific: From Sample to Structure: Crosslinking MS Made Practical - Part 2</strong></h5><ul><li>Th, 30.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6116">Registration</a></li></ul><p>Crosslinking mass spectrometry (XL-MS) is transforming how researchers map protein–protein and protein–nucleic acid interactions.</p>

Webinars LabRulezLCMS Week 18/2026

Article

Webinars

Video

Scientists

Food

Scientific article

Interview

Science and research

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Stellar Mass Spectrometer

Move biomarker candidates from discovery to validation more efficiently, confidently, and at lower cost with breakthroughs in extended scale, massive throughput, higher sensitivity, greater specificity, and unrivaled productivity.

<div><div id="container-a1c36dfacf"><div><div><div id="text-913638046"><p><strong>A new paradigm for targeted quantitation.&nbsp;Move biomarker candidates from discovery to validation more efficiently, confidently, and at lower cost with breakthroughs in extended scale, massive throughput, higher sensitivity, greater specificity, and unrivaled productivity.</strong></p><p>The Thermo Scientific Stellar Mass Spectrometer introduces revolutionary technology combining robust quantitation with the specificity of rapid full scan MS<sup>n</sup>&nbsp;acquisition creating unrivaled improvements in laboratory productivity.</p><div><div id="text-801893275"><h3><strong>Expanded experimental capabilities to meet the most challenging biomarker verification</strong></h3></div></div><div><div id="container-1e8e478f3a"><div><div><div id="text-1204752335"><p>The Thermo Scientific Stellar Mass Spectrometer offers an array of data acquisition capabilities to deliver robust and reproducible quantitation for a wide range of analytes across an extended dynamic range.</p></div></div></div></div></div><h4><strong>System highlights</strong></h4><div><div id="container-a89a56f063"><div><div><div id="text-451891225"><h5><strong>Improve ionization efficiency for more compounds at higher LC flow rates&nbsp;</strong></h5></div></div><div><div id="text-1671602085"><p>For a wider range of compounds separated using liquid chromatography operated at analytical flow rates, the Stellar Mass Spectrometer comes equipped with the Thermo Scientific OptaMax Plus Ion Source, which can deliver higher vaporizer temperatures to improve desolvation and ionization potentials.</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/725;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_Opta_Max_c303cf500a.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys OptaMax" width="1290" height="725"></figure><div><div id="container-d79c3b2946"><div><div><div id="text-251514658"><h5><strong>Spend less time on instrument set-up and focus on the science</strong></h5></div></div><div><div id="text-989104128"><p>Trigger on-demand or scheduled system calibrations remotely and without disruption to your LC-MS system with the integrated Auto-Ready ion source.</p></div></div></div></div></div><div><div id="container-81dd07c9bb"><div><div><div id="text-1289282711"><h5><strong>Improve ion utilization by extending the capabilities of the new collision cell</strong></h5></div></div><div><div id="text-1641484825"><p>The ion concentrating routing multipole (ICRM) is a curved collision cell performing both beam-type fragmentation (HCD) and precursor/product ion accumulation capabilities as controlled by dynamic automatic gain control (AGC) with synchronized ion packet release to the high-pressure linear ion trap for downstream manipulation or detection.</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/725;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_ICRM_3b2326f3af.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys ICRM" width="1290" height="725"></figure><div><div id="container-dbf5a6cdd9"><div><div><div id="text-813962034"><h5><strong>Efficiently manage ion populations for fast and sensitive analysis</strong></h5></div></div><div><div id="text-2033771024"><p>The&nbsp;hyper-fast dual-pressure linear ion trap mass analyzer expands analytical capabilities while maximizing fast, high-quality data acquisition.</p><p>The differential neutral pressures maintained in the high- and low-pressure linear ion traps prepares ions for higher-order manipulation or fast and sensitive ion ejection at scan rates up to 220 kDa/S, but typically operates at 125 kDa/S for robust data acquisition.</p></div></div></div></div></div><div><div id="container-9a523f34e0"><div><div><div id="text-1491120095"><h5><strong>Extended analytical capabilities to increase laboratory productivity</strong></h5></div></div><div><div id="text-328051555"><p>The high-pressure linear ion trap maximizes ion packet manipulation enabling multi-notch RF excitation for dissociation or ejection for SIM, higher order MS<sup>n</sup>, SPS MS<sup>3</sup>, or data-independent acquisition (DIA) to address the most challenging quantitative experiments.&nbsp;</p><div><div id="container-82e5907416"><div><div><div id="text-693866740"><h5><strong>Leverage single ion detection sensitivity to address the most challenging quantitation demands</strong></h5></div></div><div><div id="text-737565098"><p>Efficient and sensitive ion detection drives improved quantitative performance at scale. The dual conversion dynode configuration coupled to the photomultiplier tube detects ca. 84% of trapped ions for single ion sensitivity.</p></div></div></div></div></div></div></div></div></div></div><figure class="image"><img style="aspect-ratio:2048/1152;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_hmotnostni_spektrometr_SF_Sys_Extended_Scale_750dc20128.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys Extended Scale" width="2048" height="1152"></figure><div><div id="text-1993749933"><h5><strong>Automated intelligence manages tMSn acquisition</strong></h5></div></div><div><div id="text-1206797739"><p>Streamline tMSn method creation and data acquisition management. PRM Conductor software effectively and efficiently creates robust targeted quantitative methods and manages reproducible data acquisition and processing.</p></div></div><div><div id="container-b03b606507"><div><div><div id="text-2005266584"><h5><strong>Maximize data completeness with the Adaptive RT routine</strong></h5></div></div><div><div id="text-671832198"><p>Eliminate the cost and time of optimizing RT kits to the study samples to boost timed/scheduled data acquisition performance using the Adaptive RT routine.</p><p>The method leverages a novel real-time mapping routine to dynamically adjust timed/scheduled data acquisition windows at narrower time intervals to eliminate missing values that compromise data quality.&nbsp;</p></div></div></div></div></div><figure class="image"><img style="aspect-ratio:1290/654;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Scientific_Stellar_revolucni_hmotnostni_spektrometr_SF_Sys_PRM_Conductor_5b596e7e4c.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Stellar Mass Spectrometer - SF Sys PRM Conductor" width="1290" height="654"></figure><div><div id="container-a192ba4b83"><div><div><div id="container-38d4c699d2"><div><div><div id="text-1578189934"><h5><strong>Industry-leading polarity switching speeds increase laboratory productivity</strong></h5></div></div><div><div id="text-1173595596"><p>Reduce the time, cost, and challenges associated with creating and implementing highly multiplexed quantitative metabolite analysis. Fast polarity switching speeds enable comprehensive compound interrogation in +ESI and -ESI at all MS<sup>n</sup> levels on an LC timescale.</p><div><div id="container-b5fb525888"><div><div><div id="text-18006314"><h5><strong>Environmentally friendly dry pumps decrease energy usage and maintenance</strong></h5></div></div><div><div id="text-1704557304"><p>Changing from two oil-based fore pumps to a single dry pump reduces heat output by 22% and decreases maintenance.</p><p>The use of a single dry pump also eliminates oil vapors and the need for oil checks and changes.</p><h3><strong>Solutions for additional research support</strong></h3><div><h4><strong>Translational proteomics</strong></h4><p>The Stellar Mass Spectrometer offers new possibilities for translational proteomics with improved specificity for greater quantitative performance at scale.</p><h4><strong>Quantitative proteomics</strong></h4><p>Accurate and precise quantitation for label-free and TMT multiplexing sample analysis strategies with the Stellar Mass Spectrometer.</p><div><h4><strong>Metabolomics and Lipidomics</strong></h4><p>Extended analytical capabilities quantify more metabolites and lipids with greater specificity using faster liquid chromatography gradients to increase laboratory productivity.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Products from category Ion traps focused on LC/Orbitrap from Thermo Fisher Scientific - page 1

Related content

Webinars LabRulezLCMS Week 18/2026

From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility

Drug Discovery Where the Market Doesn’t Go: Targeting Neglected & Opportunistic Pathogens