Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Thermo Scientific Accucore XL C18 HPLC Columns

Accucore™ XL C18 columns deliver high-efficiency separations using rugged 4 μm solid-core particles—ideal for nonpolar analytes with excellent reproducibility and robustness.

Optimize conventional HPLC methods with Thermo Scientific™ Accucore™ XL C18 LC Columns. Rugged 4μm solid-core particles ensure very high efficiencies within standard HPLC pressure limits. The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Optimal Selectivity for Nonpolar Compounds</h5><ul><li>Hydrophobic interaction mechanism</li></ul><h5>High-Efficiency Separations for Enhanced Resolution and Sensitivity</h5><ul><li>Core-enhanced technology 4μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies for conventional HPLC methods.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Same System, Same Method, Better Results</h5><ul><li>Solid-core 4μm particle design means that no changes to the method or system are required.</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 8700 psi (600 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: C18</li><li>Surface Area: 130 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon load: 7%</li><li>Phase: Reversed phase</li><li>Pore Size: 80 Å</li><li>pH: 1 to 11</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Acclaim 120 C18 Columns

Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns.

Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns. These columns feature an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver symmetrical peak shapes. The unique bonding chemistry yields excellent surface coverage to provide highly predictable separations that are unaffected by secondary interactions. Use these high-efficiency columns for maximum resolution in a wide variety of applications.<h5>High Performance Reversed-Phase Columns for Reproducible Results</h5><ul><li>Very high surface coverage and very low metal content together result in columns with excellent efficiencies</li><li>Reproducible column-to-column performance</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 11600 psi (800 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C18</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon load: 18%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim 300 C18 HPLC Columns

Achieve high-resolution peptide mapping and protein separations with Thermo Scientific™ Acclaim™ 300 C18 LC Columns.

Achieve high-resolution peptide mapping and protein separations with Thermo Scientific™ Acclaim™ 300 C18 LC Columns. The 3μm ultrapure silica particles reduce the limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns for faster separations.<h5>High-Resolution Separation of Proteins and Peptides</h5><ul><li>High-performance C18 bonding chemistry on 300Å pore silica</li><li>Application tested for suitability in peptide mapping</li><li>Reproducible for dependable results</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 .5 and 7.5</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 6000 psi (414 bar)</li><li>Packing Material: Ultrapure Silica</li><li>Stationary Phase: C18</li><li>Surface Area: 100 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L1</li><li>Carbon load: 8%</li><li>Phase: Reversed phase</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim 120 C8 HPLC Columns

For reversed-phase separation of analytes with moderate hydrophobicity, use Thermo Scientific™ Acclaim™ 120 C8 LC columns.

For reversed-phase separation of analytes with moderate hydrophobicity, use Thermo Scientific™ Acclaim™ 120 C8 LC columns. These high-performance columns provide selectivity that is similar to Acclaim 120 C18 columns with a predictable elution order but less retention. Compounds elute more quickly, yielding faster separations. The unique chemistry of densely bonded C8 ligands on an ultrapure, spherical, porous silica substrate gives the Acclaim 120 C8 columns excellent efficiencies.<h5>High Performance Reversed-Phase Columns for the Separation of Small Molecules</h5><ul><li>Low silanol activity for excellent peak shapes</li><li>Reproducible column-to-column performance</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Porous, Ultrapure Silica</li><li>Stationary Phase: C8</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L7</li><li>Carbon load: 11%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim C30 HPLC Columns

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. 

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development.<h5>High Shape Selectivity for the Separation of Structurally Related Isomers</h5><ul><li>Unique selectivity complementary to other reversed-phase columns</li><li>High efficiency and rugged packing</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 8000 psi (550 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C30</li><li>Surface Area: 200 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L62</li><li>Carbon load: 13%</li><li>Phase: Reversed phase</li><li>Pore Size: 200 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim HILIC-10 HPLC Columns

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. 

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.<h5>Rugged and Reproducible Separations of Highly Polar Compounds:</h5><ul><li>Unique selectivity, complementary to reversed-phase columns</li><li>Enhanced sensitivity for MS detection</li><li>Hydrolytically stable</li><li>Rugged column packing</li><li>Broad application range, including separation of hydrophilic drugs and drug metabolites</li></ul>Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.<h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Silica</li><li>Surface Area: 300 m²/g</li><li>Temperature: 70°C</li><li>USP Type: N/A</li><li>Carbon load: 8%</li><li>Phase: HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Polar Advantage HPLC Columns

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns.

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes.&nbsp;In addition, the mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negates the problems of dewetting common with conventional C18 reversed-phase columns. Use Acclaim PA columns for efficient separations of nucleic acid and bases or pharmaceutical compounds using highly aqueous mobile phases.<h5>Unique Selectivity for the Separation of Polar and nonpolar Analytes</h5><ul><li>Low silanol activity for excellent peak shape with basic compounds</li><li>High selectivity for hydrophobic aromatic molecules</li><li>Compatible with 100% aqueous mobile phase</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li><li><h4>Specifications</h4></li><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar Embedded</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L60</li><li>Carbon load: 17%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Polar Advantage II HPLC Columns

Resolve polar and nonpolar compounds in a single run with Thermo Scientific Acclaim PolarAdvantage II (PA2) reversed-phase columns.

Resolve polar and nonpolar compounds in a single run with Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) reversed-phase columns. These high-efficiency, silica-based columns have a polar-embedded stationary phase that operates over a wider range of chromatographic conditions than possible with conventional reversed-phase stationary phases. Its unique chemistry provides enhanced hydrolytic stability from pH 1.5 to 10 with 100% aqueous mobile phases. The column exhibits selectivity that is complementary to conventional C18 columns and excellent peak shapes for both basic and acidic compounds.<h5>Complementary Selectivity and Enhanced Hydrolytic Stability</h5><ul><li>Compatible with 100% aqueous mobile phases</li><li>High reversed-phase capacity</li><li>Exceptional hydrolytic stability from pH 1.5 to 10</li><li>Extremely low bleed, fully compatible with mass spectrometry (MS)</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li><li><h4>Specifications</h4></li><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar Embedded</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L60</li><li>Carbon load: 17%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 1.5 to 10</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Trinity P1 HPLC Columns

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific Acclaim Trinity P1 LC Columns.

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity.The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance.<h5>Superior Performance for Pharmaceutical Applications</h5><ul><li>Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes)</li><li>Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents</li><li>Distinctive spatial separation of the anion-exchange and cation-exchange regions</li><li>Flexible method development with each retention mechanism controlled independently</li><li>Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD)</li><li>Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths</li></ul>Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.<h4>Specifications</h4><ul><li>For use with: ELSD, UV Detector</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Nanopolymer Hybrid Silica</li><li>Stationary phase: Reversed Phase/Anion Exchange/Cation Exchange</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: Reversed Phase and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Trinity P2 HPLC Columns

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific Acclaim Trinity P2 LC Columns.

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC.The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions.<h5>High Selectivity for Counterion Analysis</h5><ul><li>Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD)</li><li>Hydrolytically stable</li><li>High efficiency</li><li>Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Spherical, Fully Porous Silica</li><li>Stationary phase: Spherical, Nanopolymer Hybrid Silica</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: HILIC and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Products from category Analytical HPLC columns distributed by Thermo Fisher Scientific (EMEA) - page 3

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