Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Thermo Scientific Hypersil GOLD PEI HILIC HPLC Columns

Retain highly polar and hydrophilic analytes, which are difficult to analyze with conventional C18 columns, using Hypersil GOLD PEI HILIC LC Columns.

Retain highly polar and hydrophilic analytes, which are difficult to analyze with conventional C18 columns, using Thermo Scientific™ Hypersil GOLD™ PEI HILIC (hydrophilic interaction liquid chromatography) LC Columns. These columns deliver straightforward separation without the need for sample derivatization or ion-pair reagents. Featuring outstanding peak shape and selectivity, the columns provide improved sensitivity for MS detection. With 1.9, 3 and 5μm particle sizes, Hypersil GOLD PEI HILIC Columns are ideal for applications within drug discovery, food testing and environmental analysis.<h4>Specifications</h4><ul><li>Endcapped: No</li><li>Column type: HILIC</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Silica</li><li>Stationary Phase: Polyethyleneimine</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>Carbon Load: 6%</li><li>Phase: HILIC</li><li>Pore Size: 175 Å</li><li>pH: 2 to 8</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD Peptide Columns

Hypersil GOLD™ Peptide performance is assured by our dual quality confirmation, which is performed on both neutrals compounds as well as on a Cytochrome C digest.

<h4>Resolve peptide challenges with one column - NEW Hypersil GOLD Peptide.</h4>Achieve consistent retention of all peptides, including hydrophilic ones, and resolve deamidated species. Designed for stability and lot-to-lot consistency, this column meets the needs of biopharmaceutical product development and manufacturing control.Hypersil GOLD Peptide column performance is assured through dual quality confirmation, including a peptide-based QC test, ensures confidence in ultrapure silica, ligand bonding, and column packing for unparalleled sequence coverage and higher peak capacity compared to other peptide columns.<h5>Consistent, Reliable Results</h5><ul><li>Enjoy excellent reproducibility with minimal RSD%, ensuring consistency from column-to-column and lot-to-lot.</li></ul><h5>Optimized Peptide Retention - Retain More, Discover More</h5><ul><li>Our column uniquely holds onto hydrophilic peptides, ensuring no critical data is missed.</li></ul><h5>Exceptional Peak Capacity - Separate with Superiority</h5><ul><li>Achieve higher peak capacity for detailed and clear separations, ensuring every component is distinctly resolved.</li></ul><h5>Advanced PTM Analysis - Decipher Complex Modifications</h5><ul><li>Efficiently separate key post-translational modifications like deamidation, crucial for in-depth protein analysis. Excellent peak shape for peptides using either FA- or TFA-containing mobile phases.</li></ul><h5>Unparalleled Sequence Coverage - See the Full Picture</h5><ul><li>High sequence coverage provides a comprehensive analysis, revealing the true complexity of your samples.</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 14503 psi (1000 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>USP Type: L1</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>Carbon Load: 11%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 2 to 11</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD VANQUISH C18 UHPLC Columns

Achieve even greater power of separation, speed, and throughput using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC columns.

Achieve even greater power of separation, speed, and throughput using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC columns. These reversed-phase columns were developed in conjunction with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3">Vanquish UHPLC system</a> to take advantage of the system's extended pressure capabilities and robustness.&nbsp;The result is a new level of separation, speed and throughput that solve analytical challenges of analyzing complex matrices using liquid chromatography (LC) and LC-MS.<h4>Delivering Powerful Separations</h4>The expanded range of Accucore Vanquish, Acclaim VANQUISH, and Hypersil GOLD VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver:<ul><li>Better Separations: high efficiency enables separation of very complex mixture</li><li>More Results: High efficiency at high flow rates</li><li>Easier Interactions: Seamless workflow solution, for simple and easy separations</li><li>Wider Capabilities: Wide range of column selectivities and formats</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: C18 Selectivity</li><li>USP Type: L1</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>Carbon Load: 11%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 1 to 11</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Hypersil GOLD VANQUISH PFP UHPLC Columns

Achieve high-resolution, fast analysis of halogenated and polar compounds using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ PFP UHPLC columns for greater separation power and throughput.

Achieve even greater power of separation, speed, and throughput for analyzing difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds by using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ PFP (pentafluorophenyl) UHPLC columns. These columns were developed in conjunction with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3">Vanquish UHPLC system</a> to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges of analyzing complex matrices using liquid chromatography (LC) and LC-MS.<h4>Delivering Powerful Separations</h4>The expanded range of Accucore Vanquish, Acclaim VANQUISH, and Hypersil GOLD VANQUISH UHPLC columns offer a wider range of selectivity and format options to satisfy different UHPLC application requirements. They deliver:<ul><li>Better Separations: high efficiency enables separation of very complex mixture</li><li>More Results: High efficiency at high flow rates</li><li>Easier Interactions: Seamless workflow solution, for simple and easy separations</li><li>Wider Capabilities: Wide range of column selectivities and formats</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For use with: LC/MS</li><li>Max. Pressure: 21756 psi (1500 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: C18 Selectivity</li><li>USP Type: L1</li><li>Surface Area: 220 m²/g</li><li>Temperature: 60°C</li><li>Carbon Load: 11%</li><li>Phase: Reversed Phase</li><li>Pore Size: 175 Å</li><li>pH: 1 to 11</li></ul><h4>About the Hypersil GOLD Family of HPLC Columns</h4>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"><ul><li>Excellent peak symmetry<ul><li>Hypersil GOLD columns produce highly symmetrical peaks, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li>Narrow peaks for outstanding efficiency</li><li>Enhanced sensitivity and improved resolution</li><li>Exceptional reproducibility for reliable chromatography, column after column</li><li>Solutions for high-throughput screening</li><li>1.9, 3, and 5 μm particle sizes for all your separation needs</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925">

Thermo Scientific Accucore PFP HPLC Columns

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. 

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. Rugged 2.6μm solid-core particles enable fast, high-resolution separations at low backpressures.Fluorine groups in the PFP stationary phase provide enhanced retention and selectivity for positional isomers of halogenated compounds and for compounds with polar functional groups. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Alternative Selectivity</h5><ul><li>Pentafluorophenyl (PFP) phase provides a unique selectivity for halogenated species and nonhalogenated polar compounds.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: PFP (Pentafluorophenylpropyl)</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L43</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore Phenyl-X HPLC Columns

Achieve fast, high-resolution separations of aromatic compounds using Thermo Scientific™ Accucore™ Phenyl-X columns.

Achieve fast, high-resolution separations of aromatic compounds using Thermo Scientific™ Accucore™ Phenyl-X columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures. The phenyl-X alkyl aromatic bonded phase offers unique reversed-phase shape selectivity with high aromatic selectivity and is compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Unique Selectivity and Enhanced Retention for Aromatic Compounds</h5><ul><li>Proprietary alkyl aromatic bonded phase provides unique selectivity compared to other reversed phase materials such as C18 or hexyl.</li></ul><h5>Compatible with Highly Aqueous Mobile Phases</h5><ul><li>Specially designed bonded phase will not undergo phase collapse in highly aqueous mobile phase.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: Phenyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: N/A</li><li>Carbon Load: 6%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore HILIC HPLC Columns

Offer enhanced retention of polar and hydrophilic analytes with Thermo Scientific™ Accucore™ HILIC LC Columns.

Offer enhanced retention of polar and hydrophilic analytes with Thermo Scientific™ Accucore™ HILIC LC Columns. Based on Core Enhanced Technology™, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Enhanced Retention of Polar and Hydrophilic Analytes</li><li>Alternative Selectivity to C18 without Ion-pair or Derivatisation</li><li>Improved Sensitivity for MS detection</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>For use with: HILIC, ESI-MS</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: Silica</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L3</li><li>Phase: Normal Phase and HILIC</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore C30 HPLC Columns

Achieve fast, high-resolution separations of hydrophobic, long-chain compounds using Thermo Scientific™ Accucore™ C30 LC columns. 

Achieve fast, high-resolution separations of hydrophobic, long-chain compounds using Thermo Scientific™ Accucore™ C30 LC columns. Rugged 2.6μm solid-core particles enable fast, high-resolution separations at low backpressures.&nbsp;The C30 stationary phase offers unique shape selectivity for structurally related isomers and is compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.<h5>Unique Selectivity</h5><ul><li>High shape selectivity for hydrophobic, long-chain, structurally related isomers</li></ul><h5>Compatible with Highly Aqueous Mobile Phases</h5><ul><li>Bonded phase will not undergo phase collapse in highly aqueous mobile phase.</li></ul><h5>Fast Separations</h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5>Low Backpressures</h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5>Excellent Reproducibility and Robustness</h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: C30</li><li>Surface Area: 90 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L62</li><li>Carbon load: 5%</li><li>Phase: Reversed phase</li><li>Pore Size: 150 Å</li><li>pH: 2 to 8</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore 150 Amide HILIC HPLC Columns

Thermo Scientific™ Accucore™ 150 Amide HILIC LC Columns are ideal for separating hydrophilic biomolecules in HILIC mode, making them an excellent choice for glycan analysis.

Designed for the separation of hydrophilic biomolecules in HILIC mode, Thermo Scientific™ Accucore™ 150 Amide HILIC LC Columns are an excellent choice for glycan separations.Based on Core Enhanced Technology, Thermo Scientific Accucore columns provide fast, high-resolution biomolecule separations—without the elevated backpressures required by sub-2μm particles.<ul><li>Rugged and reproducible 2.6μm solid core particles.</li><li>Fast separations with superb resolution</li><li>Low backpressures</li><li>Amide phase bonded onto 150Å pore diameter solid core particles</li><li>High retention of a broad range of hydrophilic analytes in HILIC mode</li><li>Recommended for hydrophilic biomolecules such as glycans</li></ul><h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: Amide</li><li>Surface Area: 80 m²/g</li><li>Temperature: 60°C</li><li>USP Type: N/A</li><li>Phase: HILIC</li><li>Pore Size: 150 Å</li><li>pH: 2 to 9</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Thermo Scientific Accucore 150-C4 HPLC Columns

Offer lower hydrophobic retention, ideal for optimal retention of proteins and larger peptides with Thermo Scientific™ Accucore™ 150-C4 LC Columns. 

Offer lower hydrophobic retention, ideal for optimal retention of proteins and larger peptides with Thermo Scientific™ Accucore™ 150-C4 LC Columns. Based on Core Enhanced Technology, Thermo Scientific Accucore columns provide fast, high-resolution biomolecule separations without the elevated backpressures required by sub-2μm particles.<ul><li>150Å pore size solid core particles for fast biomoleucule separations</li><li>Superb resolution at low backpressures</li><li>Exceptionally rugged columns</li><li>Significantly lower hydrophobic retention than C18</li><li>Ideal for optimal retention of proteins and larger peptides</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Stationary Phase: C4</li><li>Surface Area: 80 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L26</li><li>Carbon load: 2%</li><li>Phase: Reversed phase</li><li>Pore Size: 150 Å</li><li>pH: 2 to 9</li></ul><h3>Accucore HPLC and UHPLC Columns</h3><h4>Resolve impurities without feeling the pressure</h4>Thermo Scientific Accucore HPLC and UHPLC columns provide ultimate core performance, allowing you to maximize your investment. Solid-core particles deliver UHPLC performance, with superb resolution delivering back-pressure compatible with HPLC instrumentation. A wide range of phases help meet all your separation needs, while our columns are exceptionally rugged and easy to use.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg" alt="Thermo Fisher Scientific: Accucore columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accucore_columns_26e0ffa8d5.jpg 156w," sizes="100vw" width="708" height="711">

Products from category Analytical HPLC columns distributed by Thermo Fisher Scientific (EMEA) - page 4

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