Derivatizing amino acids doesn’t have to take hours to do. A more hands-off solution can save your lab hours of prep work. And help you trust your data even more.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_f41f58db04.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_f41f58db04.jpeg 245w," sizes="100vw" width="1024" height="512">Ever wonder what makes a simple molecule like valine so complicated to measure?Amino acids—the building blocks of proteins—are everywhere. In your food. Body. And even the fertilizers that help grow our food. But measuring these compounds precisely? That’s a classic analytical headache, even for an experienced chromatographer.Ask any lab scientist who’s done amino acid analysis, and they’ll tell you the chromatographic separation isn’t the hard part. The challenge is sample preparation, or everything before the chromatography. Handling toxic reagents. The repeated pipetting. And then timing the derivatization reactions just right.All these manual steps can contribute to lower method precision and data reproducibility.So, what’s the solution? Using a more hands-off approach. You get the same trusted chemical reaction, but your method is now faster, cleaner, and more reliable.Let’s walk through a real-life example of how your lab can derivatize amino acids directly inside the HPLC autosampler needle.<h3>Key takeaways</h3><div><ul><li>Manual derivatization of amino acids is laborious, error-prone, and handling toxic chemicals can pose health hazards to lab staff.</li><li>Automated in-needle OPA/FMOC derivatization of amino acids is a time- and labor-saving approach for HPLC-FLD analysis.</li><li>Reliable method performance, column stability over several hundred injections, and using inexpensive reagents facilitates high sample throughput at low costs per sample for routine laboratories.</li><li>Sample matrices of medium and high complexity (beverages, food, fertilizers) using in-needle derivatization is robust and reproducible.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_2_a906250f79.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: Illustration showing manual derivatization versus in-needle derivatization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_2_a906250f79.jpeg 245w," sizes="100vw" width="700" height="410"><h3>Why does the derivatisation of amino acids still matter when aerosol detectors are an option? The unmatched sensitivity of fluorescence detection compared to evaporative technology.</h3>Most amino acids don’t have double bonds that naturally absorb UV light, meaning your HPLC detector can’t “see” the compounds directly.To solve these visibility issues, chemists use derivatization. The conjugation reaction is brilliant chemistry, facilitating the formation of <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-detectors-work.html">fluorescent derivatives</a> suitable for sensitive and precise analysis.This technique involves reacting amino acids in the solution for analysis with reagents that make the amino acids fluorescent. The most common pair are OPA (ortho-phthaldialdehyde) for primary amino acids and FMOC-Cl (9-fluorenylmethyloxycarbonyl chloride) for secondary ones.Now here’s what can go wrong. You measure reagents, mix samples by hand, wait for the reaction, then inject onto your HPLC instrument. Small inconsistencies, such as a few seconds too long or a reagent variation, can mean BIG differences in peak response, method accuracy, and data reproducibility. Removing these inconsistencies is where automated in-needle derivatization comes in.<h3>Let your HPLC autosampler needle handle the not-so-friendly laborious tasks</h3>Switching from manual to automated in-needle derivatization can help make this labor-intensive process easier for everyone in your lab, while improving data consistency and overall lab safety. &nbsp;Here’s how:<div><div><ul><li>Instead of manually preparing reaction mixtures, you can use a compatible instrument, like<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=vanquish"> Thermo Scientific Vanquish LC Systems</a>, to perform in-needle derivatization.</li><li>And, with the helpful guidance of supporting software, you can automatically mix samples and reagents in the needle, time the reaction more precisely, and inject the derivatized sample onto the column.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_3_5c636b1584.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: In-needle derivatization of primary and secondary AAs with OPA/MPA and FMOC-Cl reagents for subsequent LC analysis of the fluorescent derivatives. *Borate buffer pH 10, **acetic acid for quenching." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_3_5c636b1584.jpeg 245w," sizes="100vw" width="768" height="384"><h3>A real-life example showcasing better efficiency, robustness, and throughput capabilities.</h3>What makes this automated workflow stand out isn’t a new reaction—but rather a faster way of working with your HPLC instrument.The reactions between OPA/MPA and FMOC-Cl still happen as before, but now the reaction is controlled digitally through <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_003698_hplc_vanquish_workflow_amino_acid_analysis_derivatization_cn003698_na_en_e76e3abe44.pdf">Thermo Scientific Chromeleon Chromatography Data System</a> (CDS).As demonstrated in our whitepaper, each reagent draw, mix, and injection step is defined in a Custom Injection Program (CIP), eliminating timing errors and variability. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a> visualizes every step in its Instrument Method Editor, tracks parameters in real time, and logs events in the Audit Trail for compliance.And since the workflow design has flexibility in mind, you can use Chromeleon CDS or a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ab_004093_hplc_vanquish_core_amino_acids_needle_derivatization_ab004093_na_en_edb37df857.pdf">third-party software</a>, with identical parameters, which gives your labs the freedom to work within your preferred environment without retraining staff or rewriting SOPs.<h3>Trusting your data and a faster derivatization technique. That’s the science behind putting your sample preparation inside the needle.</h3>The most beneficial part of this workflow is the hours of time your lab and broader QC team will save.<ul><li>Consistent fluorescence response and accurate quantitation for all amino acids</li><li>Excellent injection-to-injection reproducibility, even across reagent batches</li><li>Improved safety through reduced manual solvent handling</li><li>Less hands-on time, freeing analysts for higher-value work</li><li>Seamless CDS integration for full audit traceability</li></ul>So next time you’re pipetting derivatization reagents by hand, secretly wishing there was a better way, why not consider an automated method approach?You get the same chemistry, performed automatically inside the autosampler, hands-free, ready to inject in minutes with reproducible data that you can trust.Learn more about this new automated amino acid analytical workflow in our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_003698_hplc_vanquish_workflow_amino_acid_analysis_derivatization_cn003698_na_en_e76e3abe44.pdf">customer application note today</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_4_341aed0b3a.jpeg" alt="Thermo Fisher Scientific: How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids: Chromatographic results of calibration standard (50 µM) measured with the Vanquish Core HPLC system, comparing method performance on 1st and 14th day of measurements." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_to_Cut_HPLC_Sample_Preparation_Time_for_Derivatizing_Amino_Acids_4_341aed0b3a.jpeg 245w," sizes="100vw" width="768" height="384"><h3>Helpful tips to improve method robustness with Vanquish LC Systems+ Chromeleon CDS</h3><ol><li>Use a 100 µL sample loop for thorough and reproducible mixing of the sample and reagents during the derivatization step.</li><li>Install a passive pre-heater to facilitate proper dilution of the reagents before the column.</li><li>Use guard cartridges for your columns and replace the cartridges when column performance diminishes. Apply smart tools like intelligent run control in Chromeleon CDS for automated checks.</li><li>Replace derivatization reagents in your autosampler daily.</li><li>Periodically flush your column with 100% acetonitrile for 30 minutes to remove persistent reaction products and matrices.</li></ol><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#AutomatedSamplePreparation #HPLC</blockquote></div></div></div>

How to Cut HPLC Sample Preparation Time for Derivatizing Amino Acids

<h3>How long does it actually take to install all the capillaries and fittings on a conventional HPLC system?</h3><h4>The Small Components That Matter Most</h4>When people think about HPLC systems, they often focus on pumps, detectors, columns, or software. Capillaries and fittings are usually seen as minor components because they are relatively inexpensive and physically small. However, every chromatographer eventually discovers that these tiny parts can have a major influence on system performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Connecting_a_U_HPLC_system_can_be_difficult_at_some_poorly_accessible_locations_3b837b441b.webp" alt="KNAUER: Connecting a (U)HPLC system can be difficult at some poorly accessible locations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Connecting_a_U_HPLC_system_can_be_difficult_at_some_poorly_accessible_locations_3b837b441b.webp 208w," sizes="100vw" width="720" height="540">Even decades ago, chromatographers recognized the importance of proper connections. In the 1980s, long before UHPLC became common, John Dolan wrote about minimizing dead volume through correct fitting installation. Today, with UHPLC systems operating at extremely high pressures and using columns with very small particle sizes, dead volume is even less tolerable than before. The topic remains just as relevant today as it was back then.Interestingly, despite what many users assume, the chromatographic column itself is often not the primary source of HPLC or UHPLC problems. In my experience, poor connections are far more commonly responsible for issues such as peak broadening, leaks, or loss of efficiency.<h4>The Early Days of HPLC Capillaries</h4>Anyone who has been working with HPLC for many years will probably remember the era of rigid 1/16" stainless steel tubing. Whenever PEEK tubing was unsuitable, stainless steel capillaries were the standard choice.Setting up a system back then was far from convenient. You first had to locate an appropriate length of tubing somewhere in a drawer full of capillaries. Usually, this meant finding a several-meter-long piece and cutting it yourself using a dedicated capillary cutter. After cutting, you needed to ensure the tubing end was smooth and undamaged, hoping that the previous person using the cutter had not accidentally crushed the inner diameter.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Standard_1_16_Capillary_and_needed_tools_and_the_drawer_we_all_know_pretty_well_943d7ed0b2.jpg" alt="KNAUER: Standard 1/16” Capillary and needed tools and the drawer we all know pretty well" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Standard_1_16_Capillary_and_needed_tools_and_the_drawer_we_all_know_pretty_well_943d7ed0b2.jpg 245w," sizes="100vw" width="1277" height="456">Once cut, the tubing often required additional preparation. You might need to deburr the capillary using a grater, shape it carefully with bending tools, and then finally install it using separate fittings and ferrules. And of course, everyone remembers the frustration of dropping a ferrule onto the laboratory floor, where it effectively disappeared forever.At the same time, laboratories often accumulated drawers filled with pre-assembled capillaries that had fixed fittings attached. Reusing these assemblies was risky. If the fitting geometry did not perfectly match the receiving port, users could unintentionally introduce dead volume into the system. The result was often degraded chromatographic performance, broad peaks, and frustrating troubleshooting sessions involving the entire laboratory team. In worse cases, excessive force could damage threads or receiving ports while attempting to stop leaks.During that period, assembling and connecting a complete HPLC system could easily consume half a day of work.<h4>The Improvement: Flexible and Pre-Cut Capillaries</h4>Later, laboratories gradually adopted pre-cut tubing and flexible capillaries, which represented a significant improvement over manually preparing stainless steel tubing.With these newer capillaries, users simply selected the correct length and installed the tubing directly. Flexible capillaries adapted naturally to the required shape, eliminating much of the manual bending and shaping work. Installation became faster, easier, and far less stressful.Still, these systems had limitations. Although the capillary itself improved, the fitting technology remained largely unchanged. Many of these capillaries were still effectively single-use components. If a connection was installed incorrectly because the tubing was not fully inserted into the receiving port before tightening, the result was often another unusable capillary destined for the growing “capillary drawer.” Sustainability was certainly not a strong point of these systems.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_A_typical_capillary_drawer_It_was_tricky_to_open_and_close_it_sometimes_530dd2087c.webp" alt="KNAUER: A typical capillary drawer. It was tricky to open and close it sometimes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_A_typical_capillary_drawer_It_was_tricky_to_open_and_close_it_sometimes_530dd2087c.webp 208w," sizes="100vw" width="1024" height="768">Even with these advancements, system setup still required considerable time and effort.<h4>The Real Breakthrough: Modern Tool-Free Connection Systems</h4>A major breakthrough finally arrived when manufacturers introduced a completely redesigned fitting and capillary concept aimed specifically at simplifying UHPLC connections.These newer systems came with fittings pre-installed directly on the capillary. Unlike traditional ferrule-based systems, the fittings automatically adjusted to different port depths and sealed directly at the bottom of the port. This dramatically reduced the amount of torque required and eliminated the need for complicated installation techniques.Perhaps the biggest advantage was convenience. These capillaries could be connected and disconnected more than 100 times without tools, while remaining entirely finger-tight. And best of all, ferrules no longer disappeared onto the laboratory floor.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_The_little_things_that_do_the_click_3a0469d516.jpg" alt="KNAUER: The little things that do the “click”" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_The_little_things_that_do_the_click_3a0469d516.jpg 245w," sizes="100vw" width="1866" height="1022">The system I currently use includes an especially useful feature: a patented torque-limiting mechanism that prevents both under-tightening and over-tightening. Once the correct torque is reached, the fitting produces a tactile “click,” providing immediate feedback that the connection is secure and properly installed every time.<h4>From Half a Day to Fifteen Minutes</h4>Thanks to these modern connection systems and pre-configured startup kits, installing an HPLC or UHPLC system today is dramatically faster than it used to be.What once consumed hours of work can now be completed in less than 15 minutes. The improvements in usability, reproducibility, and sustainability over recent years have been remarkable.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_e62b80154b.webp" alt="KNAUER: Trouble-free connection systom for HPLC and UHPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_e62b80154b.webp 221w," sizes="100vw" width="690" height="487"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_2_71c24acdf5.webp" alt="KNAUER: Trouble-free connection systom for HPLC and UHPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Trouble_free_connection_systom_for_HPLC_and_UHPLC_2_71c24acdf5.webp 224w," sizes="100vw" width="1920" height="1339">And while today’s solutions already represent a huge leap forward, the evolution of HPLC connection technology is certainly not over yet. Looking ahead, it will be exciting to see how future innovations continue to simplify chromatography workflows even further.<blockquote>If you want to learn more about KNAUER´s capillaries, fittings and (U)HPLC system start-up kits and are interested in details and numbers, I highly recommend our brand <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/r75ev6rjivdbid246k60zrs3gjpvwdi5b8em_fad0f92fd9.pdf">new tech note "Benefits of KNAUER´s trouble-free analytical capillaries in terms of HPLC and UHPLC system performance</a>" and a visit to our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2402">products online</a>.</blockquote>

Let´s Connect a whole (U)HPLC system without tools and trouble

News from LabRulezLCMS Library - Week 21, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Phenomenex Prodigy Phenyl-Ethyl Column

Phenomenex's ethyl-phenyl polar column is a highly reproducible reversed-phase column for the analysis of basic, acidic and amphoteric compounds.

Highly reproducible reveresed phase columns for the analysis of basic, acidic and amphoteric compounds.<ul><li>PH-3: phenyl column with excellent aromatic and polar compound selectivity</li><li>3 μm ODS-3: high efficiency alternative to the 5 μm ODS-3 columns</li><li>Columns stable from pH 2 to 9</li></ul><h5>Other Packing material:</h5><ul><li>Prodigy ODS (2)</li><li>Prodigy C8</li><li>Prodigy ODS (3)</li></ul>

Phenomenex Lux Cellulose-1 Chiral HPLC Columns

Phenomenex Lux Cellulose-1 chiral HPLC column offers reliable enantiomer separation, matching or exceeding CHIRALCEL® OD® performance across multiple modes.

This universally trusted cellulose phenyl carbamate derivative is absolutely essential to any chiral screen. Lux Cellulose-1 is a guaranteed alternative to CHIRALCEL® OD® and OD-HX. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg" alt="Phenomenex: Lux Cellulose-1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_1_Chiral_HPLC_Columns_f70a9f21a0.jpg 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Chiral</li><li>Phase Composition: Regenerated Cellulose (RC)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li><li>Stationary phase: Cellulose tris(3,5-dimethylphenylcarbamate)</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-1 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OD® and OD-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_f239557883.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_f239557883.jpg 182w," sizes="100vw" width="677" height="580">

Phenomenex Lux Cellulose-2 Chiral HPLC Columns

Phenomenex Lux Cellulose-2 chiral HPLC column, a halogenated cellulose phase, ensures superior enantiomer separation and is a proven alternative to CHIRALCEL® OZ®.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg" alt="Phenomenex: Lux Cellulose-2 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomene_Lux_Cellulose_2_Chiral_HPLC_Columns_4aa21cccda.jpg 245w," sizes="100vw" width="768" height="438"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(3-chloro-4-methylphenylcarbamate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-2 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL-OZ and OZ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations.Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects. The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π-π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg" alt="Phenomenex: Lux Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_2_bbd747a32c.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux Cellulose-3 Chiral HPLC Columns

Phenomenex Lux Cellulose-3 chiral HPLC column provides reliable enantiomer separation, performing as well or better than CHIRALCEL® OJ® with unique chiral selectivity.

This first to market halogenated cellulose phenyl carbamate derivative offers unique chiral recognition abilities that complement the rest of the Lux family of columns.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg" alt="Phenomenex: Lux Cellulose-3 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Cellulose_3_Chiral_HPLC_Columns_2_74f7d52275.jpg 216w," sizes="100vw" width="903" height="650"><ul><li>Separation Mode: Chiral</li><li>Stationary phase: Regenerated Cellulose (RC)<ul><li>Cellulose tris(4-methylbenzoate)</li></ul></li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux Cellulose-3 columns are guaranteed to perform as well or better than the equivalent CHIRALCEL® OJ® and OJ-H® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the cellulose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg" alt="Phenomenex: Lux Cellulose-3 Performance Advantage" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Performance_Advantage_cellulose_3_a232dc359a.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Cellulose-5 Chiral HPLC Columns

Phenomenex Lux i-Cellulose-5 chiral HPLC column delivers broad enantioselectivity, strong solvent compatibility, and scalable performance for LC and SFC separations.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Cellulose-5 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, immobilized with a polysaccharide stationary phase. This Immobilization provides additional strong organic solvent ruggedness so that DCM, DMSO and other related strong solvents can be used on column.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux® i-Cellulose-5 contains achemical crosslinking between the polysaccharide and silica supports which inturn provides incredible strong solvent (THF, DCM, DMSO, MtBE, Ethyl Acetate, etc.) robustness compared to coated phases and prevents the phase from being dissolved in strong solvents.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Cellulose-5 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Dependability and Seamless Scalability</h4>Our highest standards of quality will ensure that you are fully satisfied with each and every Lux chiral column as consistent quantitation and results come with every Lux batch and column that we manufacture. With matching selectivity, the 3 μm and 5 μm i-Cellulose-5 particle sizes allow you to scale down to increase resolution or easily scale up for preparative purification work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg" alt="Phenomenex: Incredible consistency batch-to-batch and between particle sizes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Incredible_consistency_batch_to_batch_and_between_particle_sizes_4a8adc47b8.jpg 103w," sizes="100vw" width="677" height="1025">

Phenomenex Lux Amylose-1 Chiral HPLC Columns

Phenomenex Lux Amylose-1 chiral HPLC column ensures reliable enantiomer separation, matching or exceeding CHIRALPAK® AD® with strong recognition and broad selectivity.

This universally trusted amylose phenylcarbamate derivative is absolutely essential to any chiral screen. Lux Amylose-1 is a guaranteed alternative to CHIRALPAK® AD®. Expect equivalent or better performance when using this Lux phase.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg" alt="Phenomenex: Lux Amylose-1 Chiral HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_Chiral_HPLC_Columns_a5e2773084.jpg 245w," sizes="100vw" width="1236" height="626"><ul><li>Stationary phase: Amylose tris(3,5-dimethylphenylcarbamate)</li><li>Recommended Use: Separation of enantiomers and racemic mixtures by reversed phase, normal phase, polar organic and SFC with a fully porous particle, coated with a polysaccharide stationary phase.</li></ul><h4>Achieve Greater Chiral Separation Success</h4>Lux columns are guaranteed to perform as well or better than the equivalent DAICEL® Chiral Technologies column. Lux phases can also provide alternative selectivity to other chiral selectors for the most challenging chiral separations. Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.The helical structure of the amylose creates a complex steric environment that provides stronger interactions and better chiral recognition abilities. Additionally, the selector’s constituents produce hydrogen bonding, dipole, and π π interactions that all affect the CSPs ability to separate your enantiomers effectively.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg" alt="Phenomenex: Separation comparison - Lux Amylose-1 vs. CHIRALPAK AD-H" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_Amylose_1_separation_c517f7e910.jpg 179w," sizes="100vw" width="677" height="590">

Phenomenex Lux i-Amylose-3 Chiral HPLC Columns

Phenomenex Lux i-Amylose-3 chiral HPLC column offers broad enantioselectivity, strong solvent stability, and proven performance as an alternative to CHIRALPAK® IG®.

<h4>Your New First Choice Chiral Column!</h4>The immobilization and bonding technology used within the Lux i-Amylose-3 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.<ul><li>Wide Solvent Flexibility</li><li>Broad Enantioselectivity</li><li>Strong Solvent Compatibility</li><li>Recommended Use: Separation of enantiomers and racemic mixtures with strong stability in strong organic solvents</li></ul><h4>Achieve Greater Chiral Separation Success</h4>The Lux i-Amylose-3, like the rest of the Lux product line, is an incredibly versatile phase that has been manufactured and developed to give you your desired results again and again. This is especially true under harsh conditions where coated phases are unable to perform.Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC puriﬁcation work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg" alt="Phenomenex: Lux i-Amylose-3 Chiral HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Lux_i_Cellulose_5_Chiral_HPLC_Columns_28bc99dbb1.jpg 245w," sizes="100vw" width="1541" height="595"><h4>Guaranteed Alternative to CHIRALPAK® IG®</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg" alt="Phenomenex: Guaranteed Alternative to CHIRALPAK® IG®" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Guaranteed_Alternative_to_CHIRALPAK_IG_584996e87b.jpg 176w," sizes="100vw" width="677" height="600">

Phenomenex Rezex RCM-Monosaccharide Ca+2 (8%) IEX HPLC Columns

Rezex RCM-Monosaccharide Ca²⁺ (8%) HPLC column (300 × 7.8 mm) enables separation of monosaccharides, sugar alcohols, and saccharides with easy regeneration and USP L19 compliance.

<h4>Key Features of the Rezex RCM-Monosaccharide Ca+2 (8%) Ion Exclusion HPLC Column:</h4><ul><li>8% cross-linked calcium</li><li>For use with monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars</li><li>Class separation of di-, tri-, and tetra- saccharides</li><li>Easy regeneration with calcium nitrate solutions</li><li>USP: L19</li></ul><h4>Specifications and Operating Recommendations</h4><ul><li>Part No.: 00H-0130-K0</li><li>Ionic Form: Calcium</li><li>Standard Dimensions: 300 x 7.8 mm</li><li>Matrix: Sulfonated Styrene Divinyl Benzene</li><li>Cross Linking: 8 %</li><li>Particle Size (μm): 8</li><li>Min. Efficiency (p/m) based on last peak: 35,000</li><li>Typical Pressure (psi @ Testing Flow Rate): 260</li><li>Max. Pressure (psi @ Max Flow Rate): 1,000</li><li>Max. Flow Rate (mL/min): 1.0</li><li>Max. Temperature (°C): 85</li><li>Typical Mobile Phase: Water</li><li>pH Range: Neutral</li><li>Guard Column Part: 03B-0130-K0</li></ul><h4>Cross Referencing Guide</h4><ul><li>Bio-Rad®Aminex®: HPX-87C (125-0095)</li><li>Supelco®SUPELCOGEL™: SUPELCOGEL (Ca)</li><li>Waters®Sugar-Pak™: Sugar-Pak 1</li><li>Transgenomic®CARBOSep™: CARBOSep (CHO-820)</li><li>Sepax®Carbomix®: Carbomix (Ca)</li></ul>

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4>Optimized Purification Media for Human Insulin and It's Analogues</h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.<h4>QC Tested to ensure consistent selectivity for Insulin and Impurity</h4>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.<h5>Key features:</h5><ul><li>Carbon Load: -</li><li>Surface Area (m²/g): -</li><li>Particle Size: 10</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Phenomenex Gemini C6-Phenyl HPLC Columns

Gemini C6-Phenyl HPLC phase provides aromatic selectivity with low bleed performance, making it ideal for LC/MS analysis of aromatic, polar, and basic compounds under high pH conditions.

Gemini C6-Phenyl HPLC phase has a phenyl group bound to the organo-silica surface using a 6-carbon chain for a low bleed aromatic selective phase. Gemini C6-Phenyl is well suited for LC/MS applications.<h5>Key features:</h5><ul><li>Carbon Load: 12 %</li><li>Surface Area (m²/g): 375</li><li>Particle Size: 3, 5</li><li>Recommended Use: Separation of aromatic, polar, or basic compounds under high pH conditions</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Products from category Analytical HPLC columns from Phenomenex - page 2

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