<div><div><div><p><strong>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in </strong><i><strong>Nature Communications</strong></i><strong>.</strong></p></div></div><div><p>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</p></div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916"></p><p>As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en"><strong>Prof. Michal Hocek</strong></a>, has now developed a technology that significantly shortens the development process.</p><p><i>“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” </i>says Marek Ondruš.</p><blockquote><p><strong>Video:</strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></p></blockquote><p>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.</p><p>The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. <i>“We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,”</i> says Marek Ondruš.</p><p>In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"></p><blockquote><h4><strong>Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas"><strong>Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</strong></a></h4><p>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal Hocek</p><p><i><strong>Nat. Commun., 2026</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y"><span>https://doi.org/10.1038/s41467-026-73676-y</span></a><span>&nbsp;</span></p><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</p></div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

<p><strong>Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.</strong></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity</strong></a><strong> 10.2 highlights</strong></h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4><strong>Instrument control modules</strong></h4><p>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"></p><h4><strong>MS data import/export improvements</strong></h4><p>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"></p><h4><strong>Obsolete hardware support terminated</strong></h4><p>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"></p><blockquote><p><strong>Explore the complete list of all changes, fixes, and additions in version 10.2.</strong></p><p><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1"><strong>See the complete changelog&nbsp;</strong></a></p></blockquote>

Clarity Chromatography Software 10.2 released!

<p><strong>🎤Dr. Leigh Terry (University of Alabama)</strong></p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.</strong></p><p>While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.</p><h4><strong>In this episode, we cover:</strong></h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted<strong> LC-MS/MS methods</strong></li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (<strong>CIC</strong>) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4><strong>Key Insight:</strong></h4><p>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.</p><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.</p><h4><strong>Understanding PFAS and Their Significance</strong></h4><h5><strong>The University of Alabama Water Quality Research Center</strong></h5><p>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.</p><p>The laboratory specializes in:</p><ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul><p>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.</p><h4><strong>Why Focus on PFAS?</strong></h4><p>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.</p><p>According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:</p><ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul><p>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.</p><h4><strong>The Development of Standardized PFAS Methods</strong></h4><p>One major advancement in PFAS analysis has been the development of standardized EPA methods.</p><p>These methods allow:</p><ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul><p>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.</p><h4><strong>How Large Is the PFAS Universe?</strong></h4><p>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.</p><p>PFOA and PFOS are often referred to as:</p><ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul><p>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.</p><p>Researchers estimate:</p><ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul><p>Scientific debate continues regarding classification and definition, making precise counting difficult.</p><h4><strong>Why PFAS Are Called "Forever Chemicals"</strong></h4><p>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.</p><p>PFAS persistence results from two key chemical properties.</p><h5><strong>1. Amphiphilic Structure</strong></h5><p>PFAS molecules contain:</p><ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul><p>This unusual combination allows PFAS to partition between:</p><ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul><p>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.</p><h5><strong>2. Extremely Strong Carbon–Fluorine Bonds</strong></h5><p>The carbon–fluorine bond is among the strongest bonds in organic chemistry.</p><p>As Dr. Terry explains:</p><ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul><p>These properties make PFAS extremely persistent in both environmental systems and biological organisms.</p><h4><strong>Toxicity, Regulations, and Environmental Behavior</strong></h4><h5><strong>Understanding PFAS Toxicity</strong></h5><p>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.</p><p>Dr. Terry explains that EPA regulations generally consider three factors:</p><ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul><p>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.</p><p>This creates important scientific questions:</p><ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul><p>These uncertainties continue to drive active research.</p><h4><strong>What Happens to PFAS in Wastewater Treatment Plants?</strong></h4><p>PFAS do not behave like many conventional contaminants.</p><p>Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:</p><ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul><p>As a result:</p><ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul><p>Their distribution depends on wastewater composition and treatment processes.</p><h4><strong>Regulatory Monitoring and Targeted LC-MS Analysis</strong></h4><p>Current EPA regulations focus on targeted analysis of specific PFAS compounds.</p><p>Examples include:</p><ul><li>PFOA</li><li>PFOS</li></ul><p>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.</p><p>Advantages of targeted LC-MS analysis include:</p><ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul><p>However, limitations remain:</p><ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul><p>As Dr. Terry notes, targeted analysis provides only part of the overall picture.</p><h4><strong>The Fluorine Mass Balance Problem</strong></h4><p>One of the most important concepts discussed in the interview is fluorine mass balance.</p><p>When PFAS degrade, fluorinated fragments may remain in the environment.</p><p>This creates a critical question:</p><p>Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?</p><p>To answer this, researchers perform fluorine mass balance calculations using:</p><ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul><p>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.</p><h4><strong>Advanced Analysis, Treatment Technologies, and Future Directions</strong></h4><h5><strong>Combining Targeted and Bulk Fluorine Measurements</strong></h5><p>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.</p><p>Approaches include:</p><ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul><p>In these methods:</p><ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul><p>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.</p><h4><strong>Surprising Findings from Combined Analysis</strong></h4><p>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.</p><p>Dr. Terry reports that:</p><ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul><p>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.</p><h4><strong>Preventing PFAS Contamination in the Laboratory</strong></h4><p>PFAS analysis requires exceptionally strict quality control.</p><p>Dr. Terry's laboratory routinely:</p><ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul><p>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.</p><h4><strong>Current PFAS Treatment Technologies</strong></h4><p>Three technologies currently dominate PFAS removal in drinking water treatment:</p><ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul><p>Selection depends on:</p><ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul><p>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.</p><h4><strong>Membrane Filtration and Pretreatment</strong></h4><p>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.</p><p>Her own work focuses on pretreatment through biological filtration.</p><p>The strategy involves:</p><ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul><p>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.</p><h4><strong>Regional Monitoring Projects</strong></h4><p>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.</p><p>Their observations indicate:</p><ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul><p>Examples include:</p><ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul><p>Source identification remains a critical component of environmental monitoring programs.</p><h4><strong>Future Directions for PFAS Monitoring</strong></h4><p>Looking ahead, Dr. Terry sees several opportunities for improvement.</p><p>Desired advances include:</p><ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul><p>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.</p><h4><strong>Dr. Terry's Perspective on Long-Term Solutions</strong></h4><p>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.</p><p>Rather than focusing solely on remediation, she emphasized prevention.</p><p>Her view is that society should:</p><ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul><p>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.</p><h4><strong>Final Thoughts</strong></h4><p>Dr. Terry encourages consumers to:</p><ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul><p>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.</p><h4><strong>Conclusion</strong></h4><p>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.</p><p>The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Galochrom Chiral ION-QD HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

<p><strong>Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QD-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Analytical Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> and ION-QD unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

<p><strong>Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QN-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Analytical Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral ION-QN and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Phenomenex Biozen Oligo LC columns

Biozen Oligo is an organo-silica, core-shell particle bonded with a C18 stationary phase. 

<p><strong>Advanced Oligonucleotide Analysis</strong></p><p><strong>Biozen Oligo</strong> is an organo-silica, core-shell particle bonded with a C18 stationary phase. This column offers a unique combination of Core-Shell performance gains such as speed, sensitivity and resolution plus the high pH ruggedness necessary for <strong>oligonucleotide separations</strong>. Additionally, Biozen Oligo is packaged in <strong>BioTi™</strong>, a novel <strong>bio-inert hardware</strong> that serves to alleviate the sample loss and adsorption issues.</p><p><strong>Increased Recovery and Reproducibility with NEW Biozen Oligo</strong></p><ul><li>BioTi™ Hardware Reduces Sample Loss and Adsorption</li><li>Robustness at High pH and Temperature</li><li>Core-Shell Advantage for High Efficiency</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_Oligo_HPLC_Column_3cf3dd7577.webp" alt="Phenomenex: Biozen Oligo HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_Oligo_HPLC_Column_3cf3dd7577.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> Reversed Phase</li><li><strong>Particle size: </strong>1.7, 2.6</li><li><strong>Recommended Use:</strong> Analytical characterization of oligonucleotides</li></ul><h4><strong>Patented Technology and Advanced Core-Shell Particle Chemistry</strong></h4><p><strong>Biozen Oligo utilizes organo-silica core-shell technology to:​</strong></p><ul><li>Minimize band broadening associated with diffusion and oligonucleotide mass transfer kinetics​</li><li>Increase efficiency to produce narrower peaks, ideal for resolving closely eluting impurities​</li><li>Patented grafting process ensures high pH and temperature resistance from ethylene cross-linking.​</li></ul><p>&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Patented_Technology_and_Advanced_Core_Shell_Particle_Chemistry_ca0e0a2b72.jpg" alt="Phenomenex: Patented Technology and Advanced Core-Shell Particle Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Patented_Technology_and_Advanced_Core_Shell_Particle_Chemistry_ca0e0a2b72.jpg 245w," sizes="100vw" width="905" height="454"></p><h4><strong>BioTi vs Stainless Steel Column Hardware​</strong></h4><p>Biozen Oligo's bioinert hardware enhances recovery, sensitivity, and chromatographic reproducibility by mitigating non-specific adsorption. Stainless steel equivalents fails to prevent this resulting in poor recovery, peak tailing, and residual carryover.​</p><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen Glycan LC columns

The unique selectivity of the Biozen Glycan was designed to provide higher order separations of released and labeled glycans. 

<p>The unique selectivity of the <strong>Biozen Glycan</strong> was designed to provide higher order separations of released and labeled glycans. With a <strong>2.6 μm core-shell particle size</strong>, customers using either <strong>HPLC</strong> or <strong>UHPLC systems</strong>, can draw upon a high efficiency Biozen Glycan particle run at higher linear velocities, to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under <strong>HILIC-FLR</strong> or <strong>HILIC-MS</strong> conditions, the Biozen Glycan excels with increased polar retention and selectivity.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_Glycan_HPLC_Column_d8ed8d30af.webp" alt="Phenomenex: Biozen Glycan HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_Glycan_HPLC_Column_d8ed8d30af.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Separation Mode:</strong> HILIC</li><li><strong>Pore Size</strong>: 100</li><li><strong>Particle size: </strong>2.6</li><li><strong>Recommended Use:</strong> High efficiency and selectivity for released glycans</li></ul><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Thermo Scientific Accucore RP-MS HPLC Columns

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.

<p><strong>Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.</strong></p><p>Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations—without the elevated backpressures required by sub-2μm particles.</p><ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Optimized for MS detection</li><li>Excellent peak shapes</li><li>Excellent combination of speed and efficiency</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Proprietary</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>USP Type: </strong>N/A</li><li><strong>Carbon Load</strong>: 7%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore C18 HPLC Columns

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

<p><strong>Achieve fast, high-resolution separations at low backpressures using Thermo Scientific™ Accucore™ C18 LC columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;</strong></p><p>The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Optimal Selectivity for Nonpolar Compounds</strong></h5><ul><li>Hydrophobic interaction mechanism</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>1 to 11</li></ul>

Thermo Scientific Accucore C8 HPLC Columns

Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. 

<p><strong>Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;</strong></p><p>The high coverage C8 bonded phase provides optimal retention of analytes with moderate hydrophobicity. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Lower Hydrophobic Retention</strong></h5><ul><li>Recommended for moderately hydrophobic analytes or for when a less hydrophobic phase provides optimum retention</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Stationary Phase:</strong> C8</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L7</li><li><strong>Carbon Load</strong>: 5%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore aQ C18 Polar Endcapped HPLC Columns

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns.

<p><strong>Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.</strong></p><p>The polar endcapped C18 stationary phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Polar endcapped C18 stationary phase provides optimum retention of polar analytes</li><li>Accucore aQ Columns are compatible with highly aqueous mobile phases.</li><li>Polar encapping allows use in 100% aqueous mobile phases without loss of performance or stability.</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Polar</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore Polar Premium HPLC Columns

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns.

<p><strong>Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.&nbsp;</strong></p><p>The robust amide-embedded C18 stationary phase provides unique selectivity complementary to conventional C18 columns, is stable from pH 1.5–10, and is fully compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Polar embedded reverse phase material offers complementary selectivity to standard C18 columns.</li></ul><h5><strong>Stable over Wide pH Range</strong></h5><ul><li>Specially designed bonded phase is stable from pH 1.5–10.5.</li></ul><h5><strong>Compatible with Highly Aqueous Mobile Phases</strong></h5><ul><li>Rugged amide-embedded C18 phase will not undergo phase collapse in 100% aqueous mobile phase.</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Polar Embedded</li><li><strong>Surface Area:</strong> 90 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L60</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>150 Å</li><li><strong>pH: </strong>1.5 to 10.5</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

<p><strong>Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.</strong></p><p>Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.</p><ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Phenyl/Hexyl</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L11</li><li><strong>Carbon Load</strong>: 5%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 8</li></ul>

Products from category Analytical HPLC columns - page 16

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