EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety

<div><div><p><strong>Researchers led by </strong><a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en"><strong>Eva Kudová</strong></a><strong> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant </strong><i><strong>Staphylococcus aureus</strong></i><strong>.</strong></p></div></div><div><p>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.</p><p>Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.</p><p>These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"></p><blockquote><h4><strong>Original Article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub"><strong>Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</strong></a></h4><p>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;</p><p><i><strong>Eur. J. Med. Chem., 2026, 308, 118716</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant <i>Staphylococcus aureus</i>. Structure-activity relationship analysis identified compounds <strong>13</strong> and <strong>16</strong> as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound <strong>13</strong> significantly downregulated <i>S. aureus</i> virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.</p><h4><strong>Instrumentation</strong></h4><h5><strong>Structural characterization</strong></h5><ul><li><strong>AVANCE III 400 MHz NMR spectrometer</strong> (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL"><strong>LTQ Orbitrap XL HRMS</strong></a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

<p><strong>Metformin is currently the first-line oral drug of choice for the treatment of type 2 diabetes. It reduces glucose production in the liver, improves tissue sensitivity to insulin, and does not cause weight gain or hypoglycemia, which sets it apart from some other antidiabetic drugs. Moreover, its long-term beneficial effects on the risk of cardiovascular complications and mortality in people with type 2 diabetes have been well established. Researchers from the Institute of Physiology CAS (IPHYS) have identified a new group of blood metabolites that could help physicians objectively monitor whether patients with type 2 diabetes are taking their metformin as prescribed.</strong></p><h4><strong>New biomarkers for metformin use</strong></h4><p>A team led by Tomáš Čajka from the Laboratory of Translational Metabolism at IPHYS analyzed plasma samples from <strong>637 patients with type 2 diabetes treated with metformin</strong> and <strong>143 non-diabetic controls</strong> using advanced metabolomics and lipidomics. Among 614 detected metabolites, they identified five from the group of <strong>N-lactoyl-amino acids </strong>(e.g. N‑lactoyl-phenylalanine), which had previously been seen as unknown signals due to missing reference spectra in databases. The levels of these metabolites in the blood closely reflected the plasma concentrations of metformin. In patients with the highest metformin exposure, the concentrations of these metabolites were up to 7.2 times higher than in controls.</p><h4><strong>A sensitive “fingerprint” of the drug’s effect</strong></h4><p>N-Lactoyl-amino acids are formed in cells by combining lactate and amino acids, thus reflecting the metformin-induced changes in cellular energy metabolism. “<i>Importantly, even though they occur in plasma only at nanomolar concentrations, they reliably distinguish patients who take metformin regularly from those who take it irregularly or not at all</i>,” emphasizes T. Čajka.</p><h4><strong>Metabolomics in practice and international collaboration</strong></h4><p>The study also provides a detailed description of broader metabolic changes in type 2 diabetes, including increases in lactate, organic acids, and branched-chain amino acids, as well as extensive remodelling of the lipid profile. In addition to T. Čajka’s team, collaborators from the Institute for <strong>Clinical and Experimental Medicine in Prague</strong> and the <strong>West Coast Metabolomics Center</strong> (University of California, Davis) also participated in the study.</p><p>The results demonstrate that advanced metabolomics approaches developed at IPHYS within the <strong>CarDia project</strong> can find highly practical applications both in clinical studies and in personalized monitoring of treatment in patients with diabetes.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://ascpt.onlinelibrary.wiley.com/doi/10.1002/cpt.70205"><strong>Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes</strong></a></h4><p>Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Veronika Hola, Stanislava Rakusanova, Oliver Fiehn, Vojtech Skop, Ivana Lankova, Iva Miskova, Terezie Pelikanova, Martin Haluzik</p><p>Clin Pharmacol Ther, 119: 1057-1069</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/cpt.70205">https://doi.org/10.1002/cpt.70205</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>Metformin is the most widely prescribed antidiabetic drug, yet adherence remains difficult to objectively assess. Using untargeted metabolomics and lipidomics, we analyzed plasma from 637 patients with type 2 diabetes (T2D) with confirmed metformin use and 143 nondiabetic controls, annotating 614 metabolites. Patients were stratified by plasma metformin into sub-therapeutic, therapeutic, and supra-therapeutic groups, and associations were evaluated by multiple linear regression and composite metabolite ranking. Five previously unannotated features were structurally identified as N-lactoyl-amino acids, whose levels correlated strongly with plasma metformin (ρ = 0.42–0.55, P &lt; 0.0001) and increased up to 7.2-fold in the supra-therapeutic group (&gt; 2000 ng/mL). While N-lactoyl-amino acids were consistently detected in the nanomolar range, they still displayed robust and dose-dependent associations with metformin. Broader metabolic changes in T2D included elevated lactate, organic acids, and branched-chain amino acids, together with reduced urea cycle metabolites. Lipidomics showed increases in saturated triacylglycerols and diacylglycerols and decreases in cholesteryl esters, sphingomyelins, and phospholipids. These findings establish N-lactoyl-amino acids as robust, dose-responsive plasma biomarkers of metformin exposure. Despite being up to four orders of magnitude less abundant than their amino acid precursors, they sensitively reflect mitochondrial lactate overflow and pharmacodynamic adaptation, offering objective assessment of adherence.</p>

New blood biomarkers reveal how faithfully diabetes patients take metformin

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The Czech Academy of Sciences (CAS) was established by Act No. 283/1992 Coll. as the Czech successor of the former Czechoslovak Academy of Sciences. It is set up as a complex of 54 public research institutions.

Czech Academy of Sciences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Thermo Scientific Accucore XL C18 HPLC Columns

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Thermo Scientific Acclaim 120 C18 Columns

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<p><strong>Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns. These columns feature an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver symmetrical peak shapes. </strong>The unique bonding chemistry yields excellent surface coverage to provide highly predictable separations that are unaffected by secondary interactions. Use these high-efficiency columns for maximum resolution in a wide variety of applications.</p><h5><strong>High Performance Reversed-Phase Columns for Reproducible Results</strong></h5><ul><li>Very high surface coverage and very low metal content together result in columns with excellent efficiencies</li><li>Reproducible column-to-column performance</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For use with: </strong>LC/MS</li><li><strong>Max. Pressure: </strong>11600 psi (800 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 300 m²/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon load:</strong> 18%</li><li><strong>Phase:</strong> Reversed phase</li><li><strong>Pore Size: </strong>120 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>About the Acclaim Family of HPLC Columns</strong></h4><p>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.</p><ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958"></p>

Thermo Scientific Acclaim 300 C18 HPLC Columns

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<p><strong>Achieve high-resolution peptide mapping and protein separations with Thermo Scientific™ Acclaim™ 300 C18 LC Columns. The 3μm ultrapure silica particles reduce the limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5μm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns for faster separations.</strong></p><h5><strong>High-Resolution Separation of Proteins and Peptides</strong></h5><ul><li>High-performance C18 bonding chemistry on 300Å pore silica</li><li>Application tested for suitability in peptide mapping</li><li>Reproducible for dependable results</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 .5 and 7.5</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For use with: </strong>LC/MS</li><li><strong>Max. Pressure: </strong>6000 psi (414 bar)</li><li><strong>Packing Material:</strong> Ultrapure Silica</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 100 m²/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon load:</strong> 8%</li><li><strong>Phase:</strong> Reversed phase</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>pH: </strong>2.5 to 7.5</li></ul><h4><strong>About the Acclaim Family of HPLC Columns</strong></h4><p>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.</p><ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958"></p>

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<p><strong>Achieve repeatable and consistent liquid chromatography (LC) separations using Thermo Scientific™ Syncronis™ C18 columns. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and ensure robust, high-resolution separations.&nbsp;</strong></p><p>Double end-capping provides extra surface coverage and inertness toward basic compounds. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.</p><h5><strong>Highly Reproducible Separations</strong></h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li><li>Double endcapped for extra surface coverage</li></ul><h5><strong>Exceptional Column-to-Column Consistency</strong></h5><ul><li>Manufactured, packed, and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5><strong>Available in Three Particle Sizes</strong></h5><ul><li>1.7 μm for rapid ultrahigh-performance LC (UHPLC) separations, and 3 μm and 5 μm for more traditional HPLC analysis</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Reversed Phase</li><li><strong>Endcapped:</strong> yes</li><li><strong>Max. Pressure:</strong> 5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> C18</li><li><strong>USP Type:</strong> L1</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load: </strong>16%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 9</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

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Thermo Scientific Syncronis aQ C18 Polar Endcapped HPLC Columns

The polar end-capped C18 phase in Thermo Scientific Syncronis aQ Column provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. 

<p><strong>Ensure consistent, predictable liquid chromatography (LC) separations of polar analytes using Thermo Scientific™ Syncronis™ aQ Columns. High surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations. The polar end-capped C18 phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.</strong></p><h5><strong>Highly Reproducible Separations</strong></h5><ul><li>Rigorously tested stationary phase bonded to the highest quality silica ensures consistent, predictable LC performance</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Dense surface coverage of silica minimizes secondary interactions and peak tailing</li></ul><h5><strong>Exceptional Column-to-Column Consistency</strong></h5><ul><li>Manufactured, packed, and tested in ISO-9000-accredited facilities</li><li>Every column individually tested to meet stringent retention, efficiency, and peak symmetry specifications</li><li>Enhanced automated packing process</li></ul><h5><strong>Alternative Selectivity</strong></h5><ul><li>Polar end-capped C18 stationary phase enhances retention of polar analytes</li></ul><h5><strong>Compatible with Highly Aqueous Mobile Phases</strong></h5><ul><li>Polar end-capping allows use of 100% aqueous mobile phases without loss of performance or stability</li></ul><h5><strong>Available in Three Particle Sizes</strong></h5><ul><li>1.7μm for rapid ultrahigh-performance LC (UHPLC) separations</li><li>3μm and 5μm for more traditional HPLC analysis</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Reversed Phase</li><li><strong>Endcapped: </strong>Polar</li><li><strong>Max. Pressure:</strong> 5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase:</strong> C18</li><li><strong>USP Type:</strong> L1</li><li><strong>Surface Area:</strong> 320 m<sup>2</sup>/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>Carbon load: </strong>19%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>Syncronis HPLC and UHPLC Columns</strong></h4><h5><strong>Enter a new world of chromatography</strong></h5><p>When developing a new method, one of the most important goals for the chromatographer is to achieve a consistent, reproducible separation. This makes selecting an HPLC column that offers highly-reproducible results essential to attaining this goal.</p><h5><strong>Features of Syncronis columns</strong></h5><p>Thermo Scientific Syncronis LC columns achieve high levels of consistency and reproducibility through:</p><ul><li>Highly-pure, high-surface-area silica</li><li>Dense bonding and double endcapping</li><li>Stringent manufacturing and testing protocols</li></ul>

Phenomenex Gemini C18 HPLC Columns

Gemini C18 is a robust reversed-phase HPLC stationary phase offering unique selectivity, high structural integrity, and increased loadability, making it ideal for preparative and purification applications.

<p><strong>Gemini C18 HPLC phase offers unique reversed phase selectivity, high structural integrity, and increased loadability which make it ideal for preparative and purification applications in pre-packed columns and bulk media.</strong></p><ul><li><strong>Carbon Load:</strong> 14, 14 %</li><li><strong>Surface Area (m²/g):</strong> 375</li><li><strong>Particle Size: </strong>3, 5, 10</li><li><strong>Recommended Use:</strong> Separation of basic compounds under high pH conditions</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

Phenomenex Gemini NX-C18 HPLC Columns

Gemini NX-C18 is a rugged, high-efficiency reversed-phase HPLC column delivering controlled selectivity for ionizable compounds and reliable performance across high and low pH LC/MS workflows.

<p><strong>The most rugged and highly efficient Gemini C18 HPLC column phase for reversed phase. Gemini NX-C18 takes LC/MS separations to the next level and offers:</strong></p><ul><li>Control selectivity of ionizable compounds for optimized methods</li><li>Consistent performance in both volatile and non-volatile buffers</li><li>High and low pH methods</li></ul><h5><strong>Key features:</strong></h5><ul><li><strong>Carbon Load:</strong>14 %</li><li><strong>Surface Area (m²/g):</strong> 375</li><li><strong>Particle Size: </strong>3, 5, 10</li><li><strong>Recommended Use:</strong> High efficiency analytical or preparative separations under extreme pH conditions</li></ul><h3><strong>Gemini HPLC Columns</strong></h3><h4><strong>Twin-NX technology HPLC columns. Extend your column lifetime</strong></h4><p>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405"></p><p>Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.</p>

KNAUER Sepapure oliGO 120-3 Column

KNAUER Sepapure oliGO 120-3 column for oligonucleotide analysis in RP and IP-RP modes with UHPLC performance and low back pressure.

<p><strong>The KNAUER Sepapure® oliGO 120-3 is a high-performance HPLC/UHPLC column optimized for oligonucleotide analysis in both reversed-phase (RP) and ion-pair reversed-phase (IP-RP) modes.</strong></p><p>The column is based on <strong>hybrid silica with advanced endcapping and hydrophobic modification</strong>, ensuring <strong>stable interactions, high reproducibility, and long lifetime</strong>, even under demanding conditions.</p><p>With <strong>3 µm particles</strong>, the column delivers <strong>UHPLC performance</strong> while maintaining <strong>low back pressure</strong>, allowing use even on standard HPLC systems.</p><p>It is ideal for:</p><ul><li><strong>identification</strong></li><li><strong>quantification</strong></li><li><strong>quality control (QC)</strong><br>of oligonucleotides.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepapure_oli_GO_120_3_Column_3_e306727053.jpg" alt="KNAUER: Sepapure oliGO 120-3 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepapure_oli_GO_120_3_Column_3_e306727053.jpg 245w," sizes="100vw" width="882" height="478"></p><h4><span><strong>Key Features</strong></span></h4><ul><li>Optimized for oligonucleotides</li><li>Supports <strong>RP and IP-RP modes</strong></li><li>UHPLC performance with low back pressure</li><li><strong>3 µm particles </strong>for high resolution</li><li><strong>18% carbon load</strong></li><li><strong>Wide pH range (1.5–10)</strong></li><li>High chemical stability</li><li>Compatible with standard HPLC systems</li><li>High reproducibility and robustness</li><li>Suitable for extreme pH and temperature conditions</li></ul><h3><span><strong>Technical Specifications</strong></span></h3><h4><span><strong>General</strong></span></h4><ul><li><strong>Separation modes:</strong> RP / IP-RP</li><li><strong>Endcapping:</strong> Yes</li><li><strong>USP classification: </strong>L1</li><li><strong>Connection:</strong> 1/16"</li></ul><h4><span><strong>Column Parameters</strong></span></h4><ul><li><strong>Particle size:</strong> 3 µm</li><li><strong>Pore size:</strong> 120 Å</li><li><strong>Surface area:</strong> 300 m²/g</li><li><strong>Carbon load:</strong> 18%</li></ul><h4><span><strong>Operating Conditions</strong></span></h4><ul><li><strong>pH range:</strong> 1.5 – 10</li><li><strong>Max pressure:</strong> 413 bar</li><li><strong>Max temperature:</strong> 60 °C</li></ul><h3><span><strong>Technology Advantages</strong></span></h3><h4><span><strong>Low Back Pressure</strong></span></h4><ul><li>enables <strong>UHPLC performance on HPLC systems</strong></li><li><strong>protects instrumentation</strong></li><li>reduces <strong>wear and clogging</strong></li><li>minimizes <strong>oligonucleotide aggregation</strong></li></ul><h4><span><strong>IP-RP Optimization</strong></span></h4><ul><li>ideal <strong>pH range: 7.0 – 9.5</strong></li><li>combines <strong>hydrophobic and ionic interactions</strong></li><li>enables <strong>high-resolution separation</strong></li></ul><h4><span><strong>Cost-effective Hardware</strong></span></h4><ul><li>no need for inert hardware</li><li>optimized for <strong>routine analytical use</strong></li><li>tested with no impact on performance</li></ul><h4><span><strong>Typical Applications</strong></span></h4><ul><li>Oligonucleotide analysis</li><li>Biopharmaceutical QC</li><li>Polar molecule analysis</li><li>Method development (extreme pH/temperature)</li></ul>

Products from category C18 HPLC columns focused on Consumables - page 1

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