<p><strong>He is one of only ten scientists working in Europe who secured the prestigious EMBO Installation Grant this year. After four years in the United States, immunologist Matouš Vobořil recently returned to the Faculty of Science at Charles University to build his own team and advance research focused on the development of immune cells and their training in the thymus. His efforts to combat autoimmune diseases have already caught the attention of numerous grant review panels.</strong></p><h5><strong>Reportedly, one of the motivations for your studies was the awareness that in science, there is still more that is unknown than what is known. You made this statement after receiving the Doctorandus Award for Natural Sciences in 2020, adding that during your lifetime you would like to shift something toward the “known” category. How has that been going so far?</strong></h5><p>The topic for which I&nbsp;take the greatest credit to date is one I&nbsp;began exploring during my doctoral studies and fully developed as a&nbsp;postdoctoral fellow in the U.S., and that is inflammation of the thymus. When you delve into the literature and articles focused on autoimmune diseases, eventually you begin to notice that autoimmune diseases often develop after undergoing some kind of infection. This is particularly evident in connection with long COVID. During inflammatory processes in the body, the immune system responds, and we were interested in whether there is a&nbsp;mechanism that recognises this inflammatory response and teaches T-lymphocytes (immune cells) that this is not a&nbsp;problem, but rather a&nbsp;process inherent to the body.</p><p>During my doctoral studies, I&nbsp;investigated the basic inflammatory response in the thymus, without the presence of actual bacterial or viral inflammation. This turned out to be extremely important. Over time, we discovered that the thymus contains many antiviral molecules and molecules that play a&nbsp;key role in allergic reactions or in the recognition of bacteria and viruses. These molecules create a&nbsp;specific environment in the thymus that mimics what may happen in our bodies later in life, and they train T-cells not to respond to these stimuli. I&nbsp;consider this to be my greatest contribution to the field. Of course, there are still many unexplored areas in this field.</p><h5><strong>The interview I was referring to was published in the magazine of the grammar school where you studied, and which proudly acknowledges your scientific achievements. Was that where your interest in science first began to show?</strong></h5><p>The fact that I&nbsp;studied in Dvůr Králové nad Labem was a&nbsp;huge stroke of luck. Not only is there a&nbsp;zoo, where my parents used to work, so I&nbsp;spent a&nbsp;lot of time there, but thanks to that, I&nbsp;also joined the local Natura club, which brought together people interested in nature and was led by the Dobroruk couple. When I&nbsp;was ten, my parents sent me to a&nbsp;nature-oriented summer camp with them, and I&nbsp;ended up staying with them – which is why I&nbsp;also attended the local high school, where Mrs. Dobroruková taught. She was a&nbsp;wonderful teacher. Her classes really encouraged students to think about what’s&nbsp;happening in nature. Thanks to that, I&nbsp;began participating in biology competitions and got involved in the Czech high school research competition. Although my first scientific experiments focused on ornithology, they gave me the opportunity to present scientific data and meet many people from the Faculty of Science at Charles University, including Professor Jan Černý, who lectured our class on immunology. Suddenly, while still in high school, I&nbsp;realized that this was the path I&nbsp;wanted to take.</p><h5><strong>During your master’s studies, you joined the team of immunologist Dominik Filipp at the Institute of Molecular Genetics of the Czech Academy of Sciences, where you then continued as a doctoral student. How did working with him influence you, and where did it lead you?</strong></h5><p>Dominik Filipp had several ongoing projects related to the peripheral immune response, signalling, and identification of viruses and bacteria. The project I worked on focused on a rare population of cells found in lymph nodes or the spleen. I studied the effect of pathogen-recognising receptors on these cells. Since these cells are similar to those in the thymus and I needed a negative control, I used the thymus for that purpose. To our surprise, the result was not negative but positive. This led us to the phenomenon itself and prompted us to begin investigating its consequences in the thymus.</p><h5><strong>You then travelled overseas to study this discovery – which you actually made rather by chance – in detail. What was your experience like at American research institutions?</strong></h5><p>Amazing. I&nbsp;went to the University of Minnesota to work with particular people, especially my future supervisor, Kristin Hogquist. I&nbsp;had met her at several conferences while I&nbsp;was still in my PhD programme, and I&nbsp;had a&nbsp;feeling that our collaboration could work well both professionally and personally.</p><p>But we were also fortunate to pick Minnesota. It’s&nbsp;a&nbsp;great place to live, and at the same time, excellent science is being conducted there. The Center for Immunology, where I&nbsp;worked, rivals the world’s&nbsp;leading scientific institutions in terms of its research results. I&nbsp;think this is mainly down to the highly supportive environment there, which fosters collaboration. Thanks to this, you can really grow as a&nbsp;scientist there.</p><p>In addition, they focus on explaining and describing the chosen phenomenon as a&nbsp;whole, rather than worrying too much about whether it will result in one or five articles, or whether it will take ten years. Their method of funding supports this approach. In a&nbsp;system of short-term grants, as we know it here, you can’t structure research this way. That’s&nbsp;a&nbsp;major disadvantage for us.</p><h5><strong>How did you develop your own research there?</strong></h5><p>It was there that I&nbsp;fully realized the potential of what we had discovered in the lab in Prague. Kristin Hogquist and her team were working on something similar, but it wasn’t until we teamed up that we were able to take it further. I&nbsp;focused primarily on how various antiviral signals or signals triggered during allergic reactions influence T-cell training and defence against autoimmune diseases following viral infections or allergic reactions. Suddenly, it became clear that this was a&nbsp;vast topic and the foundation for establishing a&nbsp;separate subfield of research on tolerance (the training of immune cells in the thymus so that they do not harm the body while remaining capable of fighting pathogens).</p><p>One great thing was that two labs were coming together at the facility: the one led by Kristin Hogquist and the other led by her husband, Stephen Jameson, which focuses on how different types of infections affect T-cell activation and their response. I&nbsp;realised that these two areas needed to be connected, and I&nbsp;based my own research plan on that – namely, to study how the inflammatory environment, actual inflammation, and viral or bacterial infections can retroactively influence processes in the thymus. This is a&nbsp;topic that no one has addressed yet.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system: As head of research, Matouš Vobořil strives to ensure that everyone on his team feels comfortable." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>You mentioned how important it was to you that you got along with Kristin Hogquist not only professionally but also on a&nbsp;personal level. To what extent did you take this “personal” aspect into account when putting together your own team at CU SCI?</strong></h5><p>This is tremendously important to me. Kristin Hogquist is not only an outstanding researcher, but also a&nbsp;very kind person. And that really helped me feel at home in the American environment, which sometimes tends to become highly competitive. I&nbsp;realized that I&nbsp;would like those who will one day work on my own team to feel the same way I&nbsp;did on hers.</p><h5><strong>So far, we’ve been talking about what led you to science. Have you, on the other hand, encountered anything during your career so far that has discouraged you from pursuing science?</strong></h5><p>Moving overseas with my family and my two-and-a-half-year-old son during the pandemic wasn’t the easiest thing to do. We had to apply for a&nbsp;national exemption just so that I&nbsp;and my family would be allowed into the U.S. at all. Fortunately, as an immunologist, I&nbsp;fell into a&nbsp;special category subject to less stringent pandemic restrictions. Packing up the family, moving overseas, and learning to navigate a&nbsp;completely different system is undoubtedly a&nbsp;major challenge. It required sacrifices not only from me, but above all from my wife, who had to put her own career on hold for several years because of mine. This is one of the biggest and most demanding obstacles that scientists and their families must overcome.</p><p>The second major obstacle you have to deal with as a&nbsp;scientist is paperwork – something I’ve only fully realized now that I’m leading a&nbsp;research group. Even though you hear about it from those around you, it still doesn’t prepare you for when it comes. As a&nbsp;trainee, you do science and conduct experiments in the lab, but suddenly everything shifts, and you become a&nbsp;manager and administrator of all sorts of things. I&nbsp;spend about 30 percent of my time on paperwork. It’s&nbsp;not exactly fun.</p><h5><strong>You obtained a&nbsp;Primus grant, a&nbsp;Junior Star grant from the Czech Science Foundation, and most recently an EMBO Installation Grant. What research challenges do you now face with these grants?</strong></h5><p>I&nbsp;would also like to apply for an ERC Starting Grant this year as well. In the Junior Star project, we are studying cell migration to the thymus. We hypothesize that so-called dendritic cells migrate to the thymus from the periphery (skin, lungs, intestines) – that is, from sites exposed to the external environment and inflammation. These cells are thought to bring information about their own antigens to the thymus and teach developing T-cells tolerance. We aim to determine whether this migration actually occurs, under what circumstances, and how important it is for establishing tolerance to specific tissues. This could help answer the question of why autoimmune skin diseases, such as psoriasis, develop following inflammatory processes.</p><p>The second objective is broader. We would like to answer a&nbsp;major question in immunology: <strong>why do autoimmune diseases develop after a&nbsp;person has had an infection? </strong>We want to study how different types of inflammation (viral, bacterial, or yeast infections) affect the training of immune cells in the thymus.</p><p>We would like to introduce a&nbsp;system of so-called “dirty mice.” The entire field of immunological research is based on working with mice raised in sterile conditions so that we can easily manipulate them and observe how these manipulations affect the immune system. In reality, however, the immune system does not function this way. I&nbsp;brought this idea back from the University of Minnesota, where they placed standardized mice in the same cage with mice purchased from various pet stores around Minneapolis. After a&nbsp;few weeks to months, the mice contract almost everything the store-bought mice have, thereby developing a&nbsp;true physiological immune response. Yet they are still the same mice – genetically identical – that we can manipulate. This is an excellent system that allows us to study immune responses in a&nbsp;much more physiological context.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>Will you be applying for a&nbsp;European Research Council (ERC) grant with that goal in mind?</strong></h5><p>Exactly.</p><h5><strong>In order to succeed in the competition for ERC grants, the projects should push the boundaries of knowledge, which also involves a&nbsp;certain degree of risk. Where do you see the greatest risks in your own research?</strong></h5><p>There are many risks. One of the biggest is that although all the infections we plan to introduce are controlled and well-documented, you still never have full control over them. So it’s&nbsp;possible that not only will the mice become peripherally infected, but the thymus itself will also become infected, which could complicate the experiments. At the same time, this is a&nbsp;physiological system – and I’m talking mainly about the “dirty mice” – which means that each mouse will respond a&nbsp;little differently, and that will generate enormous variability in the samples. Immunologists strive to have the purest system possible, so that when they introduce, for example, a&nbsp;single infectious agent, they can then observe exactly what’s&nbsp;happening there. In this regard, we are actually taking a&nbsp;step back, so to speak, because we want it to be more variable and closer to reality. Setting up and analysing data from such experiments so that they’re reproducible and reveal a&nbsp;real phenomenon can be a&nbsp;real challenge. But that’s&nbsp;the only way we can get closer to real life.</p><h5><strong>The most recent Nobel Prize in Physiology or Medicine was awarded to three immunologists: Mary E. Brunkow, Fred Ramsdell, and Shimon Sakaguchi, who recently gave a&nbsp;lecture in Prague. Did you meet him? How much did awarding the Nobel Prize to immunologists motivate you further in your work?</strong></h5><p>When Shimon Sakaguchi was in Prague, I&nbsp;was still in the U.S., but I&nbsp;did meet him several times at various conferences and even had the opportunity to talk with him on multiple occasions, given that we’re both involved in T-cell research. In our community, people have long been placing bets on when they’ll finally get the Nobel Prize. For me, their discoveries are truly inspiring, but the Nobel Prize itself doesn’t motivate me that much anymore. I&nbsp;find the story behind it to be more interesting. It took a&nbsp;very long time for the scientific community to accept their findings. One of the seminal articles was published in the <i>Journal of Immunology</i>, which isn’t a&nbsp;journal with as high a&nbsp;profile as <i>Nature</i> or <i>Science,</i> and not one where you’d expect to read about such a&nbsp;groundbreaking discovery. It took several years before it became clear just how significant this discovery was. Now I&nbsp;often remind my students of this – even an article in the <i>Journal of Immunology</i> can win you a&nbsp;Nobel Prize, and that is incredibly inspiring.</p><blockquote><h5><strong>Matouš Vobořil, PhD</strong></h5><p>Graduate of the Faculty of Science at Charles University. His research focuses on the development of immune cells in the thymus, the onset of autoimmune diseases, and the function of antigen-presenting cells. After spending four years as a&nbsp;postdoctoral fellow at the University of Minnesota on Kristin Hogquist’s&nbsp;team, he returned to Europe in 2025. Thanks to a&nbsp;Primus grant from Charles University, he established his own research group at the Faculty of Science. He subsequently received a&nbsp;Junior Star grant from the Czech Science Foundation and, this January, an EMBO Installation Grant. For his dissertation, he was awarded the Doctorandus Prize as part of the Česká hlava initiative.</p></blockquote>

Vobořil: There is still much to learn about the immune system 

<h3><strong>How are PFAS regulations changing, and what role will ion chromatography play in the future of environmental testing?</strong></h3><p>In this episode, &nbsp;David interviews <strong>Jay Gandhi, PhD, </strong>Vertical Markets Manager at <strong>Metrohm USA</strong>, for an in-depth discussion on PFAS analysis, the rise of AOF (Adsorbable Organic Fluorine) testing, and the development of EPA 1621 and ISO 18127 standards.</p><p>With more than 30 years of experience in ion chromatography, Jay shares valuable perspective on how the field has evolved—from early applications and NASA water recovery projects to today’s challenges involving PFAS, TFA, and emerging contaminants.</p><h4><strong>Topics covered in this episode include:</strong></h4><ul><li>What PFAS are and why they matter</li><li>The difference between targeted PFAS methods and AOF analysis</li><li>How combustion ion chromatography works</li><li>Behind the scenes of EPA 1621 and ISO 18127 method development</li><li>Regulatory trends in water, textiles, and food packaging</li><li>TFA analysis using IC-MS and IC-MS/MS</li><li>The future of ion chromatography in environmental labs</li></ul><h3><strong>Video Transcription</strong></h3><p>For more than three decades, Jay Gandhi has worked with ion chromatography (IC), watching the technique expand from a specialized tool for water analysis into an analytical platform used across environmental testing, food and beverage analysis, semiconductor manufacturing, battery applications, and, increasingly, PFAS analysis.</p><p>In an interview for <i>Concentrating on Chromatography</i>, Gandhi discusses his introduction to IC during his work on a NASA Mars exploration project, the emergence of PFAS as a major analytical challenge, and why techniques such as adsorbable organic fluorine (AOF) determination and combustion ion chromatography (CIC) can provide information that targeted LC-MS analysis alone cannot deliver. He also examines the development of standardized methods, sample preparation challenges, and the future role of IC-MS and total fluorine approaches in PFAS monitoring.</p><h4><strong>From NASA to ion chromatography</strong></h4><p>Gandhi's introduction to ion chromatography came through his work as a principal scientist on a NASA Mars exploration project focused on water recovery under zero-gravity conditions. IC played an important role because it enabled researchers to establish the ionic balance of anions and cations in water.</p><p>The technology was used both to investigate micronutrients relevant to agricultural applications and to evaluate water purity for potential astronaut consumption. During approximately three years of this work, Gandhi gained extensive experience with ion chromatography. This ultimately led him deeper into IC instrumentation and, 24 years ago, to joining Metrohm.</p><p>At the beginning of his career, IC was strongly associated with relatively straightforward anion and cation analysis in water. The technique itself had been used for water analysis since the 1970s, but Gandhi has seen its application range expand dramatically.</p><p>Food and beverage testing and environmental analysis became important fields, supported by both direct and indirect detection strategies. More recently, IC has also found applications in areas such as the semiconductor industry and battery recycling.</p><p>In Gandhi's words, ion chromatography has effectively "bloomed" into numerous application areas.</p><h4><strong>PFAS changes the analytical challenge</strong></h4><p>PFAS represent a particularly important chapter in this development. These chemicals have been used for decades in numerous consumer and industrial products, including non-stick materials, firefighting foams, water-resistant textiles and other applications.</p><p>Gandhi recalls work on PFOA and PFOS using ion chromatography with conductivity detection as early as 2008. However, the achievable detection limits were insufficient for the extremely low concentrations that later became important in PFAS analysis.</p><p>A major turning point for him came in 2015, when he encountered research from Sweden using <strong>combustion ion chromatography to investigate total fluorine</strong>. Subsequent collaboration with researchers at Clarkson University and Harvard University, particularly on aqueous film-forming foam (AFFF), further demonstrated the importance of looking beyond individual targeted PFAS compounds.</p><h4><strong>Why targeted LC-MS does not tell the whole story</strong></h4><p>Targeted LC-MS is highly sensitive and selective and can provide detailed information about individual PFAS compounds. However, according to Gandhi, the number of possible PFAS structures in the environment is vastly greater than the number routinely included in targeted methods.</p><p>A targeted method may monitor dozens or perhaps around one hundred compounds, while the overall PFAS universe can include thousands of chemical structures. Consequently, targeted analysis may characterize only a small fraction of the total PFAS burden.</p><p>There are other practical considerations. LC-MS measurements can be affected by matrix-dependent ion suppression, while accurate quantification frequently relies on isotope-labelled internal standards. Such standards are available for only a limited number of compounds and can be expensive.</p><p>This is where measurements of total or adsorbable organic fluorine can provide complementary information. Rather than asking <i>which specific PFAS compounds are present?</i>, these approaches can help answer a broader question: <i>how much fluorine associated with this chemical space is present in the sample?</i></p><h4><strong>AOF as a complementary approach</strong></h4><p>Adsorbable organic fluorine analysis provides a different perspective from targeted LC-MS. In the workflow discussed by Gandhi, organic fluorine compounds are captured during sample preparation and subsequently measured using combustion ion chromatography.</p><p>The principle of CIC builds upon older total organic halogen analysis. The sample is combusted at temperatures above approximately 1,000 °C, breaking carbon-halogen bonds. In PFAS-related analysis, carbon-fluorine bonds are broken and the resulting fluoride is collected in an absorption solution.</p><p>Ion chromatography is then used to separate and quantify the fluoride.</p><p>Replacing older detection approaches with IC provides both sensitivity and selectivity for the halogens being measured. Effective combustion is critical: oxygen supports the combustion process, while a carrier gas transports the products into the absorption solution for subsequent IC analysis.</p><h4><strong>The challenge of inorganic fluoride</strong></h4><p>The broad response of CIC is simultaneously one of its strengths and one of its analytical challenges. Combustion does not distinguish fluorine originating from PFAS from fluorine present in inorganic compounds such as fluoride salts.</p><p>This becomes particularly important when analyzing water containing relatively high concentrations of inorganic fluoride while attempting to quantify organic fluorine at very low levels.</p><p>Sample preparation therefore has a critical role. Inorganic fluoride must be effectively separated from the fraction of interest, while materials used during sample preparation must themselves have sufficiently low fluoride backgrounds.</p><p>Contamination can come from unexpected sources. Gandhi points out that background fluoride may originate not only from reagents and sample preparation materials but potentially from the laboratory environment. Careful blank control, appropriate materials and rigorous evaluation of interferences are therefore essential when moving toward increasingly low detection limits.</p><h4><strong>From research method to international standardization</strong></h4><p>The development of standardized AOF methods illustrates the importance of collaboration between academia, industry, regulatory organizations and analytical laboratories.</p><p>Gandhi describes work that began in Europe around 2015 and subsequently progressed through German standardization activities, ISO development, ASTM-related collaboration and the U.S. EPA. The process eventually contributed to methods such as <strong>EPA Method 1621</strong>.</p><p>He emphasizes that this was not the work of a single scientist or organization. Developing a robust method required years of experiments, discussions and sometimes difficult technical debates among numerous stakeholders.</p><p>For Gandhi, the essential principle was that experimental evidence ultimately had to determine the outcome. Collaboration becomes possible when scientists focus on the data rather than the organizations they represent.</p><h4><strong>PFAS monitoring is moving beyond drinking water</strong></h4><p>Regulatory attention to PFAS is also expanding beyond drinking water. Gandhi describes a broader strategy that begins with reducing the manufacture and use of PFAS-containing materials and continues with removing existing contamination from the environment.</p><p>Wastewater represents an important part of this picture because PFAS can enter it from numerous sources. Historical contamination from landfills and contaminated sites can also migrate into groundwater and ultimately affect drinking-water supplies.</p><p>Consumer products are another area of increasing attention. Food packaging, disposable containers, automotive fabrics, outdoor clothing and water-repellent textiles have historically used fluorinated substances to provide properties such as grease, water or stain resistance.</p><p>Reducing PFAS use in such products could therefore gradually decrease the amount entering waste streams and the wider environment.</p><h4><strong>Screening first, regulation later?</strong></h4><p>Gandhi expects AOF to be particularly valuable as a <strong>screening tool</strong>, providing an estimate of broader fluorine contamination before laboratories proceed to more specific analyses.</p><p>Its importance becomes clear when AOF results are compared with targeted PFAS measurements. Gandhi reports that laboratories working with EPA Method 1621 have observed differences between targeted PFAS results and broader AOF measurements ranging from approximately <strong>300- to 4,000-fold in the total fluorine mass balance</strong>.</p><p>Such discrepancies suggest that targeted methods can leave a substantial portion of fluorine unexplained.</p><p>Rather than competing with LC-MS, AOF and CIC therefore provide complementary information. A broad screening method can reveal the magnitude of total contamination, while targeted mass spectrometry identifies and quantifies specific compounds of interest.</p><h4><strong>Where IC-MS fits into the PFAS toolbox</strong></h4><p>No single technique can cover the entire PFAS problem. Gandhi highlights very short-chain, highly polar fluorinated compounds as another analytical challenge.</p><p>Compounds such as trifluoroacetic acid (TFA) are highly water-soluble and may not be quantitatively retained by the carbon materials used for AOF sample preparation. Direct ion chromatography can provide an alternative route for separating such compounds from common inorganic anions.</p><p>Coupling IC with mass spectrometry adds another level of sensitivity and selectivity. Gandhi therefore sees <strong>IC-MS and IC-MS/MS</strong> as increasingly important tools for highly polar fluorinated compounds. Similar approaches can also be useful beyond PFAS, including the determination of polar pesticides such as glyphosate and glufosinate.</p><h4><strong>Toward lower detection limits and more complete PFAS analysis</strong></h4><p>For Gandhi, current standardized methods represent a beginning rather than an endpoint. Improvements in instrumentation and particularly sample preparation could push total fluorine analysis to substantially lower concentrations.</p><p>He notes that current combustion IC configurations can reach approximately <strong>0.5 ppb</strong>, or 500 parts per trillion, and believes that improvements in sample volume, preparation procedures and mitigation of inorganic fluoride backgrounds could eventually move detection below 100 ppt and potentially toward 50 ppt.</p><p>Extractable organic fluorine workflows could provide another bridge between broad fluorine screening and targeted LC-MS methods. Combining improved extraction with CIC could allow samples to be screened for organic fluorine before more detailed compound-specific characterization.</p><p>The broader message from Gandhi's career is that PFAS analysis is unlikely to be solved by a single analytical technique. <strong>Targeted LC-MS provides compound-specific sensitivity and selectivity; AOF and combustion IC reveal a broader fluorine picture; and IC-MS can address highly polar compounds that are difficult to capture using conventional workflows.</strong> Used together, these techniques can provide laboratories with a much more complete understanding of PFAS contamination than any one approach alone.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

<p><strong>In late February 2025, a train carrying benzene derailed in Hustopeče nad Bečvou, and within hours, a race was on to save the groundwater and surface water. One of the most toxic industrial substances began seeping into the gravel-sand subsoil. While fieldwork was underway to prevent the spread of contamination, decisions were already being made in the CXI TUL laboratories regarding how to subsequently clean up the contaminated rock environment.</strong></p><p>The accident ranks among the <strong>most serious environmental incidents in the Czech Republic in recent years</strong>. After a tanker train derailed, hundreds of<strong> metric tons of benzene leaked</strong>; due to the highly permeable geological subsoil, it quickly seeped into deeper soil layers and into the groundwater. The combination of the substance’s high toxicity and local conditions complicated both the emergency response and the subsequent remediation. The key was to stop further spread, determine the extent of the contamination, and establish a remediation strategy for the entire site.</p><p>It was at this stage that CXI TUL became involved in the solution. At the request of the renowned remediation company DEKONTA, a.s., a team led by <strong>RNDr. Jan Němeček, Ph.D.</strong>, began testing the feasibility of the<strong> in situ chemical oxidation (ISCO) method</strong>. This technology is designed to<strong> break down benzene directly within the rock matrix</strong>, without the need for extensive excavation of contaminated soil or the pumping out of large volumes of groundwater. The goal was simple: to find a method that would work quickly, safely, and under the actual conditions of the affected site.</p><h4><strong>Benzene Remediation: Technically Simple, Complicated in Practice</strong></h4><p>Benzene is a highly toxic and carcinogenic substance that rapidly penetrates into groundwater in permeable subsoil, where it dissolves and spreads further as the water flows toward surface water bodies. Benzene also binds to rock particles and migrates in the form of vapors into overlying unconsolidated layers.</p><p>Scientists in Liberec therefore tested <i>in situ </i>chemical oxidation (ISCO) technology under laboratory conditions. The goal was to find an oxidant capable of breaking down benzene directly underground into less hazardous substances.</p><p>The tests evaluated two main options:<strong> activated hydrogen peroxide and sodium peroxodisulfate</strong>. Both substances removed up to<strong> 99.99% of the benzene</strong>, but there was a difference in their kinetics and impacts on the natural environment. Activated hydrogen peroxide achieved nearly complete degradation of the contaminant within a single day. Activated sodium peroxodisulfate was slower and also significantly altered the chemistry of the water; it acidified the environment and increased the sulfate content in the water, which poses an additional environmental burden.</p><p>For field applications, the researchers therefore unequivocally recommended <strong>activated hydrogen peroxide</strong>.</p><h4><strong>Dosage Matters</strong></h4><p>It was not just the oxidant’s effectiveness that was key. It was equally important to determine how the chemical reaction would behave in a real geological environment. The team of scientists therefore also analyzed what is known as <strong>soil oxidant consumption (SOD)</strong>. It turned out that a significant portion of the oxidizing agent does not react solely with benzene but is also consumed to a significant extent by the rock environment itself, particularly by natural organic substances present in the fine-grained fraction of the soil.</p><p>An oxidant consumption value was determined specifically for the local composition of the aquifers, which is essential for the correct dosing of chemicals in the field so that the remediation is not only effective but also economically viable and safe for surrounding ecosystems.</p><h4><strong>One Year After the Accident: Research Turns into Real-World Assistance</strong></h4><p>While the public often perceives university science as a distant world of laboratories and grant projects, it is precisely situations like this that demonstrate its practical impact. The results of the work by researchers at CXI TUL did not remain confined to academic studies but directly influenced decisions regarding the technology used to clean up one of the largest environmental accidents in recent years.</p>

Science on the Front Lines: Cleanup of a Benzene Spill

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Phenomenex Biozen dSEC-1 LC columns

Phenomenex Biozen dSEC-1 is a high-resolution SEC column for peptide therapeutics, delivering fast, reproducible aggregate analysis with improved peak shape and compatibility with SEC-MS and SEC-MALS.

<h4><strong>High-Resolution Aggregate Analysis for Peptide Therapeutics</strong></h4><p>Biozen dSEC-1 columns are engineered to meet the growing demand for aggregate analysis in peptide therapeutics and mid-sized biologics. Packed with 90 Å small pore silica and featuring inert diol-based surface chemistry, dSEC-1 delivers enhanced resolution for low and high molecular weight peptide aggregates. Its robust design ensures reproducibility and compatibility with advanced workflows like SEC-MS and SEC-MALS.</p><h5><strong>Robust Performance for Peptide Aggregate Analysis</strong></h5><ul><li>High resolution for low/high MW peptide aggregates (1–50 kDa)</li><li>Reproducible, and fast analysis with extended column lifetime</li><li>Improved peak asymmetry by up to 40%</li><li><strong>Recommended Use: </strong>Analysis of peptides, protein fragments, and small biologics with reduced analysis times and improved column lifetimes.</li></ul><h4><strong>Aggregate and Monomer Separation in Liraglutide Formulations</strong></h4><ul><li>Baseline resolution of monomer and two aggregates achieved in under 10 minutes</li><li>Exceptional reproducibility with retention time RSD ≤ 0.08% and peak area RSD ≤ 0.01%</li><li>Clear detection at low levels with S/N ratios of 13, 75, and 10,917 for aggregates and monomer</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_separation_in_liraglutide_formulations_3_b0d97cd01f.jpg" alt="Phenomenex: Aggregate and Monomer Separation in Liraglutide Formulations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_separation_in_liraglutide_formulations_3_b0d97cd01f.jpg 245w," sizes="100vw" width="938" height="307"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_separation_in_liraglutide_formulations_2_035bfea3bc.jpg" alt="Phenomenex: Aggregate and Monomer Separation in Liraglutide Formulations" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_separation_in_liraglutide_formulations_2_035bfea3bc.jpg 245w," sizes="100vw" width="1700" height="1050"></p><h3><strong>Biozen SEC Proprietary Silica Particle Technology</strong></h3><p>The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg" alt="Phenomenex: Biozen SEC Proprietary Silica Particle Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_SEC_Proprietary_Silica_Particle_Technology_502218b910.jpg 245w," sizes="100vw" width="1448" height="469"></p><h4><strong>Improved Column Lifetime and Performance Stability</strong></h4><p>Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.</p><h4><strong>New Standard for Platform SEC Methods</strong></h4><p>Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.</p><h3><strong>Phenomenex Biozen Biologics LC Columns</strong></h3><p>With a new titanium <strong>BioTi™</strong> biocompatible <strong>hardware</strong> to minimize priming, <strong>four particle platforms</strong> for optimal versatility and <strong>nine particle chemistries</strong> to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for <strong>protein analysis</strong> are seamlessly designed to bring peace of mind to your analysis of biologics through:</p><ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3><strong>Biocompatible Flow Path with BioTi™ Hardware</strong></h3><p>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"></p><h3><strong>4 Advanced Particle Platforms</strong></h3><p>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868"></p>

Phenomenex Biozen N-Glycan Clean-Up

Biozen N-Glycan Clean-up enables fast, efficient SPE purification of labeled, released N-glycans using HILIC microelution, ideal for small sample volumes and LC-MS workflows.

<h4><strong>Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)</strong></h4><p>SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.</p><ul><li>Small volume format allows for more concentrated samples</li><li>Minimal elution solvent</li><li>Excellent retention and recovery of labeled, released N-glycans</li><li>Faster clean-up methods to save on sample prep time</li></ul><h4><strong>Protocol</strong></h4><ul><li><strong>96-Well Plate: </strong>Biozen N-Glycan Clean-up</li><li><strong>Part No.: </strong>8M-S009-NGA</li><li><strong>Condition: </strong>200 µL Water</li><li><strong>Equilibrate:</strong> 200 µL Water/Acetonitrile (15:85)</li><li><strong>Load:</strong> Labeled Sample (up to 400 µL of labeled sample)</li><li><strong>Wash: </strong>2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)</li><li><strong>Elute: </strong>3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)</li><li><strong>Dilute:</strong> 100 µL Dimethylformamide and then 210 µL of Acetonitrile</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_N_Glycan_962a23eee6.jpg" alt="Phenomenex: Biozen N-Glycan Clean-up" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_N_Glycan_962a23eee6.jpg 245w," sizes="100vw" width="1444" height="424"></p>

Phenomenex Clarity OTX Clean-Up

Rapid 15-minute clean-up of biological samples using Clarity OTX SPE enables efficient isolation of RNA- and DNA-based therapeutics without LLE, optimized for LC-MS bioanalysis.

<h4><strong>15 Minute Clean Up from Biological Samples</strong></h4><p><strong>Isolate RNA and DNA-based therapeutics / metabolites from biological fluids and tissues in less time with Clarity OTX solid phase extraction (SPE) and buffer products.</strong></p><ul><li>No liquid-liquid extraction (LLE) required required</li><li>&gt;80 % typical extraction recoveries</li><li>Optimized for LC-MS bioanalysis</li></ul><h3><app-cb-header style="color:rgb(65, 66, 69);font-family:proxima-nova, sans-serif;font-size:16px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:left;text-indent:0px;text-transform:none;"><div><div><h2 style="border-bottom:1px solid rgb(51, 51, 51);color:rgb(0, 41, 72);font-size:32px;font-style:normal;font-weight:400;line-height:1.2;"><div>General Extraction</div></h2></div></div></app-cb-header><app-cb-fourtext-eightimage style="color:rgb(65, 66, 69);font-family:proxima-nova, sans-serif;font-size:16px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:left;text-indent:0px;text-transform:none;"><div><div><div style="flex-wrap:wrap;"><div style="flex:0 0 100%;"><h2 style="color:rgb(0, 41, 72);font-size:32px;font-weight:400;line-height:1.2;">Common Extraction Procedure (~180 minutes)</h2></div></div></div></div></app-cb-fourtext-eightimage><strong>General Extraction</strong></h3><h4><strong>Common Extraction Procedure (~180 minutes)</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Clarity_OTX_Clean_Up_e317411382.jpg" alt="Phenomenex: Clarity OTX Clean-Up: Common Extraction Procedure (~180 minutes)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Clarity_OTX_Clean_Up_e317411382.jpg 245w," sizes="100vw" width="678" height="374"></p><h5><strong>Suitable for Most Oligo Therapeutics &amp; Samples</strong></h5><p><strong>Oligo types / Sample Types</strong></p><ul><li>DNA: Plasma</li><li>Aptamers: Serum</li><li>RNAi/siRNA: Urine</li><li>Thioates: Tears</li><li>Lipid-conjugates: Saliva</li><li>Liposome encapsulated: Tissue</li></ul><h4><strong>Extraction Protocol</strong></h4><p><strong>Sample Pre-Treatment</strong></p><ul><li><strong>Tissue:</strong><ul><li>Proteinase K digestion or homogenize</li><li>Mix 1-to-1 with Lysis-Loading buffer</li></ul></li><li><strong>Biological Fluids: </strong>Mix 1-to-1 with Lysis-Loading buffer</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Clarity_OTX_Clean_Up_2_fd322c63cd.jpg" alt="Phenomenex: Clarity OTX Clean-Up: Extraction Protocol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Clarity_OTX_Clean_Up_2_fd322c63cd.jpg 245w," sizes="100vw" width="1031" height="494"></p>

Phenomenex Phenex LC/GC Approved Syringe Filters

Phenex syringe filters deliver fast, reliable sample filtration with excellent flow rates and broad chemical compatibility. Designed to protect columns, reduce downtime, and ensure particulate-free samples for LC, GC, and MS analysis.

<p><strong>Phenex syringe filters are designed for efficient and rapid filtration of almost any solution prior to analysis, and are optimized for superior flow rates and high-throughput. Proper syringe filter membrane and size selection is the key to choosing the best product to maintain the integrity of your sample components as well as to protect your system from particulate contamination. Phenex offers a wide variety of membranes ideal for any application.</strong></p><h5><strong>Try our Phenex Syringe Filters for:</strong></h5><ul><li>High quality filtration product</li><li>Increased column lifetime</li><li>Less system downtime</li><li>Low protein absorption</li></ul><h4><strong>Phenex Offers:</strong></h4><ul><li>Broad chemical compatibility</li><li>Minimized extractables</li><li>Excellent flow rate</li><li>High total throughput</li><li>Certified quality</li><li>100 % integrity tested</li><li>Low hold-up volume</li><li>Low protein adsorption</li><li>Bi-directional use</li></ul><h3><strong>Maximum Quality</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_2_44145fa074.jpg" alt="Phenomenex: Phenex LC-GC Approved Syringe Filters: Maximum Quality" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_2_44145fa074.jpg 218w," sizes="100vw" width="1156" height="828"></p><h5><strong>A: Identification</strong></h5><ul><li>Syringe filter membrane type and pore size are clearly marked on each syringe filter</li></ul><h5><strong>B: Luer Lock Inlet Tip</strong></h5><ul><li>Secures connections to prevent “blow off”</li></ul><h5><strong>C: Sample Distribution Rings</strong></h5><ul><li>Creates even sample distribution for high sample flow rates</li></ul><h5><strong>D: Medical Grade Polymer Housing</strong></h5><p>Offers the most inert syringe filter and helps eliminate unwanted secondary interactions with the filter housing</p><h5><strong>E: Ultrasonically Welded</strong></h5><ul><li>Ensures robust housing - filter integrity</li></ul><h4><strong>Guaranteed Reliability and Consistency</strong></h4><p>Quality filtration products from Phenomenex offer a convenient way to clean up your samples - for improved analytical results with a simple method. Phenex syringe filters are designed for efficient and rapid filtration of almost any solution prior to analysis, and are optimized for superior flow rates and high-throughput. Phenex offers a wide variety of membranes ideal for any application.</p><ul><li>High quality filtration product</li><li>Increased column lifetime</li><li>Less system downtime</li></ul><p>The housing attaches to any standard luer lock syringe, so the sample can easily be pushed through the membrane with minimal pressure. The result is a particulate-free eluent that is ready for use with LC, GC, or other analytical techniques. One of the most popular sample preparation methods is filtration using a sample preparation solution. It has the ability to remove particulates before injection onto the column and the ability to use it in parallel with another sample preparation technique. It requires no method development.</p><ul><li><strong>A:</strong> Particulates Trapped</li><li><strong>B:</strong> Particulate-free Sample</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_631acccb54.jpg" alt="Phenomenex: Phenex LC/GC Approved Syringe Filters: Guaranteed Reliability and Consistency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_631acccb54.jpg 42w," sizes="100vw" width="143" height="523"></p><h3><strong>Recommendation Based on Your Industry</strong></h3><h5><strong>Environmental</strong></h5><p>Water, wastewater, soil and sludge, and pollution control samples are especially challenging. No matter the sample type, Phenex offers filtration products to meet your demanding requirements.</p><ul><li><strong>Recommended Filter: </strong>GF/NY</li><li><strong>First Alternative:</strong> RC</li></ul><h5><strong>Pharmaceutical / Biotech</strong></h5><p>At every stage of the drug discovery process target compounds must be isolated, purified, and prepared prior to testing. Sample complexity in DMPK work can be even more challenging. Difficult samples such as serum, urine, and other physiological fluids are easily filtered and clarified using Phenex syringe filters.</p><ul><li><strong>Recommended Filter: </strong>PES</li><li><strong>First Alternative: </strong>RC</li></ul><h5><strong>Clinical / Toxicology</strong></h5><p>Removal of particulate matter to sub-micron levels is critical before any clinical sample is injected into an LC, GC or mass spectrometer. At every stage in toxicology, samples should be filtered, preferably with a rapid and simple sample preparation method to reduce downtime in the fast paced environment. Phenex is designed for higher flow rates and throughputs than those of competing products.</p><ul><li><strong>Recommended Filter: </strong>RC</li><li><strong>First Alternative: </strong>PES</li></ul><h5><strong>Food and Beverage</strong></h5><p>Food safety is more important than ever and lower detection limits are making analysis even more challenging. Accurate and reliable testing is critical and Phenex filters are routinely used in preparation for analysis of pesticides, herbicides, fungicides, flavors, and fragrances. For samples with large amounts of particulate and/or large fibrous matter, use a glass fiber prefilter.</p><ul><li><strong>Recommended Filter: </strong>GF/NY</li><li><strong>First Alternative: </strong>RC</li></ul><h3><strong>How to Use Phenex Syringe Filters</strong></h3><h4><strong>Easy to assemble and even easier to get consistent and particulate-free samples</strong></h4><h5><strong>Step 1: Load</strong></h5><ul><li>Fill a syringe barrel with the liquid sample. Allow a small amount of air (approximately 10 % of the sample volume) to enter the syringe. The air is used as a purge to minimize fluid retention when expelling the sample from the syringe (Step 3 below).</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_1_f8246ba832.jpg" alt="Phenomenex: Phenex LC/GC Approved Syringe Filters - Step 1: Load" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_1_f8246ba832.jpg 155w," sizes="100vw" width="423" height="427"></p><h5><strong>Step 2: Assemble</strong></h5><ul><li>Twist the luer lock end of the filter securely onto the syringe.</li><li><strong>Caution: </strong>Do not use syringes without a matching luer lock, otherwise the pressure applied may cause the filter to come off unexpectedly.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_2_2833584d34.jpg" alt="Phenomenex: Phenex LC/GC Approved Syringe Filters: Step 2: Assemble" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_2_2833584d34.jpg 158w," sizes="100vw" width="349" height="344"></p><h5><strong>Step 3: Filter</strong></h5><ul><li>Apply gentle pressure to the syringe plunger.</li><li><strong>Caution: </strong>Small syringes can generate excessive pressures.) Push the liquid sample, as well as the remaining air, through the syringe filter to maximize sample recovery.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_3_d8c5f61231.jpg" alt="Phenomenex: Phenex LC/GC Approved Syringe Filters - Step 3: Filter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phenex_LC_GC_Approved_Syringe_Filters_Step_3_d8c5f61231.jpg 177w," sizes="100vw" width="375" height="331"></p>

KNAUER Sepapure oliGO 120-3 Column

KNAUER Sepapure oliGO 120-3 column for oligonucleotide analysis in RP and IP-RP modes with UHPLC performance and low back pressure.

<p><strong>The KNAUER Sepapure® oliGO 120-3 is a high-performance HPLC/UHPLC column optimized for oligonucleotide analysis in both reversed-phase (RP) and ion-pair reversed-phase (IP-RP) modes.</strong></p><p>The column is based on <strong>hybrid silica with advanced endcapping and hydrophobic modification</strong>, ensuring <strong>stable interactions, high reproducibility, and long lifetime</strong>, even under demanding conditions.</p><p>With <strong>3 µm particles</strong>, the column delivers <strong>UHPLC performance</strong> while maintaining <strong>low back pressure</strong>, allowing use even on standard HPLC systems.</p><p>It is ideal for:</p><ul><li><strong>identification</strong></li><li><strong>quantification</strong></li><li><strong>quality control (QC)</strong><br>of oligonucleotides.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepapure_oli_GO_120_3_Column_3_e306727053.jpg" alt="KNAUER: Sepapure oliGO 120-3 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepapure_oli_GO_120_3_Column_3_e306727053.jpg 245w," sizes="100vw" width="882" height="478"></p><h4><span><strong>Key Features</strong></span></h4><ul><li>Optimized for oligonucleotides</li><li>Supports <strong>RP and IP-RP modes</strong></li><li>UHPLC performance with low back pressure</li><li><strong>3 µm particles </strong>for high resolution</li><li><strong>18% carbon load</strong></li><li><strong>Wide pH range (1.5–10)</strong></li><li>High chemical stability</li><li>Compatible with standard HPLC systems</li><li>High reproducibility and robustness</li><li>Suitable for extreme pH and temperature conditions</li></ul><h3><span><strong>Technical Specifications</strong></span></h3><h4><span><strong>General</strong></span></h4><ul><li><strong>Separation modes:</strong> RP / IP-RP</li><li><strong>Endcapping:</strong> Yes</li><li><strong>USP classification: </strong>L1</li><li><strong>Connection:</strong> 1/16"</li></ul><h4><span><strong>Column Parameters</strong></span></h4><ul><li><strong>Particle size:</strong> 3 µm</li><li><strong>Pore size:</strong> 120 Å</li><li><strong>Surface area:</strong> 300 m²/g</li><li><strong>Carbon load:</strong> 18%</li></ul><h4><span><strong>Operating Conditions</strong></span></h4><ul><li><strong>pH range:</strong> 1.5 – 10</li><li><strong>Max pressure:</strong> 413 bar</li><li><strong>Max temperature:</strong> 60 °C</li></ul><h3><span><strong>Technology Advantages</strong></span></h3><h4><span><strong>Low Back Pressure</strong></span></h4><ul><li>enables <strong>UHPLC performance on HPLC systems</strong></li><li><strong>protects instrumentation</strong></li><li>reduces <strong>wear and clogging</strong></li><li>minimizes <strong>oligonucleotide aggregation</strong></li></ul><h4><span><strong>IP-RP Optimization</strong></span></h4><ul><li>ideal <strong>pH range: 7.0 – 9.5</strong></li><li>combines <strong>hydrophobic and ionic interactions</strong></li><li>enables <strong>high-resolution separation</strong></li></ul><h4><span><strong>Cost-effective Hardware</strong></span></h4><ul><li>no need for inert hardware</li><li>optimized for <strong>routine analytical use</strong></li><li>tested with no impact on performance</li></ul><h4><span><strong>Typical Applications</strong></span></h4><ul><li>Oligonucleotide analysis</li><li>Biopharmaceutical QC</li><li>Polar molecule analysis</li><li>Method development (extreme pH/temperature)</li></ul>

KNAUER Sepapure Laboratory Filtration

Sepapure laboratory filtration includes syringe filters, membranes, and filter units for clean, precise, and reproducible results.

<p><strong>The Sepapure® Laboratory Filtration range provides a comprehensive solution for laboratory sample filtration, focusing on purity, precision, and reproducibility.</strong></p><p>The portfolio includes:</p><ul><li><strong>syringe filters</strong></li><li><strong>membrane disc filters</strong></li><li><strong>filter units</strong></li><li><strong>filter holders</strong></li></ul><p>These products are designed to ensure <strong>maximum sample recovery, minimal loss, and broad compatibility</strong> across analytical, pharmaceutical, and routine laboratory applications.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>High filtration purity and reproducibility</li><li>Minimal sample loss</li><li>Color-coded identification</li><li>Wide range of membrane materials</li><li>Broad application compatibility</li><li>Pressure resistance (&gt;6 bar)</li><li>Eco-friendly reusable solutions</li></ul><h3><span><strong>Product Variants</strong></span></h3><h4><span><strong>Sepapure® Syringe Filters</strong></span></h4><ul><li>Color-coded and labeled</li><li>Integrated polypropylene prefilter</li><li>Low hold-up volume → minimal sample loss</li><li>High pressure resistance (&gt;6 bar)</li><li>Wide application compatibility</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepapure_Laboratory_Filtration_5_00f6e96bbe.jpg" alt="KNAUER: Sepapure® Syringe Filters" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepapure_Laboratory_Filtration_5_00f6e96bbe.jpg 245w," sizes="100vw" width="1216" height="196"></p><h4><span><strong>Sepapure® Membrane Disc Filters</strong></span></h4><ul><li>Various membrane materials</li><li>Efficient separation for complex samples</li><li>High filtrate purity</li><li>Suitable for lab and research use</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepapure_Laboratory_Filtration_3_1e207905e0.jpg" alt="KNAUER: Sepapure® Membrane Disc Filters" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepapure_Laboratory_Filtration_3_1e207905e0.jpg 156w," sizes="100vw" width="1000" height="1000"></p><h4><span><strong>Filter Units (Glass Vacuum Units)</strong></span></h4><ul><li>Glass vacuum filtration systems</li><li>Available volumes: 1000 mL and 2000 mL</li><li>Ground glass or rubber joints</li><li>Compatible with membrane filters</li><li>High chemical resistance and durability</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Sepapure_Laboratory_Filtration_2_86cf0cc5f8.jpg" alt="KNAUER: Filter Units (Glass Vacuum Units)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Sepapure_Laboratory_Filtration_2_86cf0cc5f8.jpg 156w," sizes="100vw" width="1000" height="1000"></p><h4><span><strong>Filter Holders</strong></span></h4><ul><li>Reusable and durable</li><li>Eco-friendly (waste reduction)</li><li>Versatile applications</li><li>High mechanical stability</li></ul><h4><span><strong>Typical Applications</strong></span></h4><ul><li>Sample preparation for HPLC/LC-MS</li><li>Mobile phase filtration</li><li>Environmental analysis</li><li>Pharmaceutical QC</li><li>Biochemical and clinical workflows</li></ul>

KNAUER Vials & Caps

KNAUER vials and caps for HPLC/UHPLC with high chemical resistance, multiple closure types, and optimized solutions for precise sample handling.

<p><strong>The KNAUER Vials &amp; Caps range provides a complete solution for sample preparation in HPLC/UHPLC analysis, where precision, purity, and sample integrity are critical.</strong></p><p>Selecting the correct vial and cap combination directly impacts:</p><ul><li><strong>result reproducibility</strong></li><li><strong>contamination prevention</strong></li><li><strong>minimization of evaporation</strong></li><li><strong>autosampler performance</strong></li></ul><p>KNAUER offers a wide variety of configurations including:</p><ul><li>glass types (clear / amber)</li><li>closure types (screw, crimp, snap)</li><li>septa (pre-slit / non pre-slit)</li><li>accessories (µ-inserts for small volumes)</li></ul><h3><span><strong>Product Variants</strong></span></h3><h4><span><strong>Sample Vials</strong></span></h4><ul><li><strong>Neck types:</strong><ul><li>screw neck</li><li>crimp neck</li><li>snap ring</li></ul></li><li><strong>Materials:</strong><ul><li>clear glass</li><li>amber glass (UV protection)</li></ul></li><li>High chemical resistance</li><li>Compatible with micro-inserts</li></ul><h4><span><strong>Caps</strong></span></h4><ul><li>Compatible with all vial types</li><li><strong>Options:</strong><ul><li>pre-slit septa</li><li>solid septa</li></ul></li><li>Optimized sealing</li><li>Suitable for automated and manual sampling</li></ul><h3><span><strong>Vial Selection Guide</strong></span></h3><h4><span><strong>Glass Color</strong></span></h4><ul><li><strong>Amber glass</strong><ul><li>UV protection</li><li>ideal for <strong>light-sensitive compounds (vitamins, steroids, pharmaceuticals)</strong></li></ul></li><li><strong>Clear glass</strong><ul><li>maximum visibility</li><li>ideal for <strong>routine applications</strong></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Vials_and_Caps_glass_color_bd1238185e.jpg" alt="KNAUER: Vials &amp; Caps - Glass color" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Vials_and_Caps_glass_color_bd1238185e.jpg 155w," sizes="100vw" width="435" height="437"></p><h4><span><strong>Closure Types</strong></span></h4><ul><li><strong>Screw Neck</strong><ul><li>resealable</li><li>ideal for <strong>volatile samples and storage</strong></li></ul></li><li><strong>Crimp Neck</strong><ul><li>tightest seal</li><li>ideal for <strong>highly volatile samples</strong></li></ul></li><li><strong>Snap Ring</strong><ul><li>tool-free handling</li><li>suitable for <strong>short-term use</strong></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Vials_and_Caps_closure_type_c6c7a61418.jpg" alt="KNAUER: Vials &amp; Caps - Closure type" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Vials_and_Caps_closure_type_c6c7a61418.jpg 245w," sizes="100vw" width="978" height="389"></p><h4><span><strong>Septum Types</strong></span></h4><ul><li><strong>Pre-slit septum</strong><ul><li>easier needle penetration</li><li>ideal for <strong>autosamplers</strong></li><li>prevents vacuum formation</li></ul></li><li><strong>Non pre-slit septum</strong><ul><li>tighter seal</li><li>ideal for <strong>storage and evaporation control</strong></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Vials_and_Caps_septum_type_0b6afde0e9.jpg" alt="KNAUER: Vials &amp; Caps - Septum type" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Vials_and_Caps_septum_type_0b6afde0e9.jpg 245w," sizes="100vw" width="551" height="343"></p><h4><span><strong>µ-Inserts (Small Volumes)</strong></span></h4><ul><li>Reduce sample volume requirements</li><li>Maximize recovery</li><li>Ideal for valuable or limited samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Vials_and_Caps_3_b697f0f41b.webp" alt="KNAUER: Vials &amp; Caps - Micro inserts" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Vials_and_Caps_3_b697f0f41b.webp 239w," sizes="100vw" width="1000" height="653"></p>

KNAUER Capillaries & Tubing

KNAUER Capillaries & Tubing provide high-pressure LC capillaries and tubing up to 1200 bar, including MarvelXACT™ tool-free UHPLC connection systems.

<p><strong>KNAUER Capillaries &amp; Tubing provide a wide range of capillaries and tubing solutions for analytical and preparative HPLC, UHPLC, and FPLC applications. The portfolio includes stainless steel, PEEK, PTFE, and other tubing materials designed for reliable LC connections at pressures up to 1200 bar. The range also features innovative MarvelXACT™ capillaries with tool-free fingertight connections and a patented torque-limiting mechanism for secure and trouble-free operation.</strong></p><h4><span><strong>Key Features</strong></span></h4><ul><li>Capillaries and tubing for HPLC, UHPLC, and FPLC</li><li>Maximum pressure up to 1200 bar</li><li>Materials include stainless steel, PEEK, PTFE, PFA, ETFE, and more</li><li>Suitable for analytical and preparative applications</li><li>MarvelXACT™ fingertight connection system</li><li>Patented torque-limiting “click” mechanism</li><li>Automatic adjustment to different port depths</li><li>More than 100 connection/disconnection cycles</li><li>PEEK capillaries for maximum biocompatibility</li><li>User-cuttable standard capillaries</li><li>Tubing for low-pressure solvent and waste lines</li></ul><h4><span><strong>MarvelXACT™ Capillaries</strong></span></h4><ul><li>Recommended for AZURA® Analytical systems</li><li>Tool-free installation</li><li>Flexible 1/32” OD tubing</li><li>compatible with KNAUER standard 1/16” receiving ports</li><li>Pre-installed fittings</li><li>Eliminates under- and over-tightening</li><li>Patented torque-limiting mechanism</li><li>Flexible tubing for easy routing</li><li>Bottom-port sealing without ferrules</li></ul><h4><span><strong>Available Options</strong></span></h4><ul><li>Stainless steel capillaries for high-pressure applications</li><li>PEEK capillaries for biocompatible workflows</li><li>PTFE, PFA, ETFE, and Tefzel® tubing</li><li>User-customizable capillary lengths</li><li>Tubing for solvent and waste connections</li></ul>

KNAUER Pipettes

KNAUER Pipetting Solutions combine ergonomic BlueOrchid pipettes, high-purity pipette tips, reload systems, and sustainable rack boxes for accurate, comfortable, and contamination-free liquid handling workflows.

<p><strong>KNAUER Pipetting Solutions combine ergonomic design, precision engineering, and high-quality consumables to support reliable liquid handling workflows in analytical, biological, and routine laboratory applications. The portfolio includes BlueOrchid micropipettes, premium pipette tips, reload systems, and sustainable rack boxes designed to deliver maximum accuracy, user comfort, and workflow efficiency.</strong></p><h4><span><strong>BlueOrchid Pipettes</strong></span></h4><p>KNAUER BlueOrchid pipettes are designed for effortless daily pipetting with reduced hand strain and reliable long-term performance. The lightweight and balanced construction, combined with a silicone-based shock absorption mechanism and magnet-assisted piston system, minimizes pipetting and tip ejection forces for fatigue-free operation.</p><p>The pipettes feature intuitive one-handed volume adjustment with a secure locking mechanism and a large 4-digit display for easy readability. All models are ISO 8655-6 calibrated to ensure high accuracy and precision.</p><p><strong>Available single-channel volume ranges include:</strong></p><ul><li>0.1–2.5 µL</li><li>0.5–10 µL</li><li>2–20 µL</li><li>5–50 µL</li><li>10–100 µL</li><li>20–200 µL</li><li>100–1,000 µL</li><li>500–5,000 µL</li><li>1,000–10,000 µL</li><li>Fixed-volume 150 µL model</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Pipettes_6_3d401a1c67.jpg" alt="KNAUER: Pipettes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Pipettes_6_3d401a1c67.jpg 241w," sizes="100vw" width="1046" height="677"></p><p><strong>Key characteristics:</strong></p><ul><li>Ergonomic low-force pipetting design</li><li>Color-coded volume identification</li><li>Autoclavable and chemically resistant construction</li><li>UV-resistant materials</li><li>No recalibration required after sterilization</li><li>Compatible with KNAUER pipette tips and many third-party brands</li></ul><h4><span><strong>Pipette Tips</strong></span></h4><p>KNAUER pipette tips are manufactured in Germany from high-quality medical-grade virgin polypropylene to ensure contamination-free and highly reproducible pipetting. The product range includes eight <strong>tip sizes from 10 µL to 10 mL</strong>, including <strong>extra-long 10 µL tips</strong> optimized for recovery of very small sample volumes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Pipette_Tips_71e87b2e4b.webp" alt="KNAUER: Pipette Tips" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Pipette_Tips_71e87b2e4b.webp 100w," sizes="100vw" width="690" height="1077"></p><p>The tips are designed with optimized cone geometry for secure pipette fitting and low-force attachment and ejection. A conical tip opening and ultra-smooth internal surface reduce droplet formation and minimize sample retention.</p><p><strong>Key advantages:</strong></p><ul><li>Free of DNase, RNase, human DNA, pyrogens, and PCR inhibitors</li><li>Autoclavable and non-wettable</li><li>US FDA-approved virgin polypropylene</li><li>Universal compatibility with major pipette brands</li><li>Graduation marks for rapid volume verification</li><li>Available in bulk bags or reload systems</li><li>Yellow and blue versions available for selected tip sizes</li></ul><p><strong>Available packaging options:</strong></p><ul><li>Bulk bags for economical refilling</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Bulk_Bags_f99e73dba3.webp" alt="KNAUER: Bulk Bags" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Bulk_Bags_f99e73dba3.webp 156w," sizes="100vw" width="690" height="690"></p><ul><li>Reload units for fast one-handed box refilling and reduced plastic waste</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Reloads_48b0e2cf9d.webp" alt="KNAUER: Reload units" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Reloads_48b0e2cf9d.webp 245w," sizes="100vw" width="690" height="343"></p><h4><span><strong>Reload Systems</strong></span></h4><p>KNAUER reload systems are designed to simplify laboratory workflows while reducing environmental impact. <strong>Reloads</strong> are available for <strong>10 µL, 200 µL, and 1,000 µL pipette tips</strong> and allow quick replacement of empty rack inserts with minimal handling effort.</p><p><strong>Advantages of reload systems:</strong></p><ul><li>One-handed reload operation</li><li>Reduced plastic consumption</li><li>Space-saving storage</li><li>Color-coded rack inserts for quick identification</li><li>Optimized for routine high-throughput workflows</li></ul><h4><span><strong>Rack Boxes</strong></span></h4><p>KNAUER rack boxes provide secure and sustainable storage for pipette tips. The boxes are manufactured from 100% recycled plastic sourced from pipette tip production and are fully autoclavable.</p><p>The stackable design allows safe and space-efficient storage, while the easy-open lid and color-coded rack inserts improve laboratory organization and workflow efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Tip_boxes_1d59517540.webp" alt="KNAUER: Rack boxes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Tip_boxes_1d59517540.webp 245w," sizes="100vw" width="1024" height="427"></p><p><strong>Available rack box versions:</strong></p><ul><li>10 µL / 10 µL XL pipette tips</li><li>200 µL / 300 µL pipette tips</li><li>1,000 µL pipette tips</li><li>1,250 µL pipette tips</li></ul><p><strong>Key features:</strong></p><ul><li>Made from 100% recycled plastic</li><li>Stackable construction</li><li>Secure tip positioning</li><li>Easy one-handed handling</li><li>Compatible with single- and multi-channel pipettes</li><li>Sustainable and reusable design</li></ul>

Phenomenex Luna Omega HILIC HPLC Columns

Phenomenex Luna Omega HILIC combines a bonded diol phase with fully porous particles for superior retention and selectivity of polar analytes in HILIC applications.

<p><strong>Luna Omega HILIC columns combine thermally modified fully porous particles with a bonded diol stationary phase to provide enhanced retention and selectivity for polar analytes under HILIC conditions. Designed for small polar molecules, metabolites, nucleobases, pharmaceuticals, and other challenging compounds, these columns deliver excellent chromatographic performance and robustness.</strong></p><p>The diol stationary phase provides multiple interaction mechanisms that improve retention and separation of polar compounds while maintaining excellent peak shape and reproducibility. The thermally modified fully porous particles extend column lifetime and ensure reliable performance across demanding analytical workflows.</p><h3><strong>Technical Specifications</strong></h3><h4><strong>Stationary Phase</strong></h4><ul><li><strong>Phase: </strong>Diol</li><li><strong>USP Classification:</strong> L20</li></ul><h4><strong>Particle Characteristics</strong></h4><ul><li><strong>Particle Sizes:</strong><ul><li>1.6 µm</li><li>3 µm</li></ul></li><li><strong>Pore Size:</strong> 100 Å</li><li><strong>Surface Area:</strong> 260 m²/g</li><li><strong>Carbon Load:</strong> 5 %</li></ul><h4><strong>Chemical Properties</strong></h4><ul><li><strong>pH Range: </strong>2.0–7.0</li><li><strong>Maximum Pressure: </strong>1034 bar (15,000 psi)</li></ul><h4><strong>Available Dimensions</strong></h4><ul><li><strong>Lengths:</strong><ul><li>50 mm</li><li>100 mm</li><li>150 mm</li><li>250 mm</li></ul></li><li><strong>Internal Diameters:</strong><ul><li>2.1 mm</li><li>3.0 mm</li><li>4.6 mm</li></ul></li></ul><h4><strong>Typical Applications</strong></h4><ul><li>HILIC separations</li><li>Nucleobases</li><li>Nucleotides</li><li>Metabolomics</li><li>Pharmaceutical analysis</li><li>Bioanalytical workflows</li><li>Food analysis</li><li>Environmental testing</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_Omegs_HILIC_b02079eee2.jpg" alt="Phenomenex: Separation of Nucleobases with Luna Omega 3μm HILIC.  1. Thymine 2. Uracil 3. Adenine 4. Cytosine 5. Guanine" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_Omegs_HILIC_b02079eee2.jpg 245w," sizes="100vw" width="1284" height="648"></p><h3><strong>Phenomenex Luna Omega HPLC/UHPLC Columns</strong></h3><p>The cutting edge thermally modified Luna Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.</p><ul><li>Greater separation muscle and retention</li><li>Consistent porous structure and elimination of micropores</li><li>Extreme ruggedness and reproducibility</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg" alt="Phenomenex:Thermal modified pore structure of Luna Omega Column particles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg 245w," sizes="100vw" width="1257" height="369"></p><h3><strong>Explore Luna Omega Columns</strong></h3><h5><strong>Astounding Performance</strong></h5><p>The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.</p><h5><strong>Separation Muscle</strong></h5><p>Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.</p><h5><strong>Inert Foundation</strong></h5><p>Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.</p><h5><strong>Consistent Results</strong></h5><p>Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.</p>

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