KNAUER Sepapure oliGO 120-3 Column

The KNAUER Sepapure® oliGO 120-3 is a high-performance HPLC/UHPLC column optimized for oligonucleotide analysis in both reversed-phase (RP) and ion-pair reversed-phase (IP-RP) modes.

The column is based on hybrid silica with advanced endcapping and hydrophobic modification, ensuring stable interactions, high reproducibility, and long lifetime, even under demanding conditions.

With 3 µm particles, the column delivers UHPLC performance while maintaining low back pressure, allowing use even on standard HPLC systems.

It is ideal for:

• identification
• quantification
• quality control (QC)
  of oligonucleotides.

KNAUER: Sepapure oliGO 120-3 Column

Key Features

• Optimized for oligonucleotides
• Supports RP and IP-RP modes
• UHPLC performance with low back pressure
• 3 µm particles for high resolution
• 18% carbon load
• Wide pH range (1.5–10)
• High chemical stability
• Compatible with standard HPLC systems
• High reproducibility and robustness
• Suitable for extreme pH and temperature conditions

Technical Specifications

General

• Separation modes: RP / IP-RP
• Endcapping: Yes
• USP classification: L1
• Connection: 1/16"

Column Parameters

• Particle size: 3 µm
• Pore size: 120 Å
• Surface area: 300 m²/g
• Carbon load: 18%

Operating Conditions

• pH range: 1.5 – 10
• Max pressure: 413 bar
• Max temperature: 60 °C

Technology Advantages

Low Back Pressure

• enables UHPLC performance on HPLC systems
• protects instrumentation
• reduces wear and clogging
• minimizes oligonucleotide aggregation

IP-RP Optimization

• ideal pH range: 7.0 – 9.5
• combines hydrophobic and ionic interactions
• enables high-resolution separation

Cost-effective Hardware

• no need for inert hardware
• optimized for routine analytical use
• tested with no impact on performance

Typical Applications

• Oligonucleotide analysis
• Biopharmaceutical QC
• Polar molecule analysis
• Method development (extreme pH/temperature)