<div><div><div><p><strong>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in </strong><i><strong>Nature Communications</strong></i><strong>.</strong></p></div></div><div><p>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</p></div><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916"></p><p>As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en"><strong>Prof. Michal Hocek</strong></a>, has now developed a technology that significantly shortens the development process.</p><p><i>“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” </i>says Marek Ondruš.</p><blockquote><p><strong>Video:</strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></p></blockquote><p>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.</p><p>The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. <i>“We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,”</i> says Marek Ondruš.</p><p>In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"></p><blockquote><h4><strong>Original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas"><strong>Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</strong></a></h4><p>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal Hocek</p><p><i><strong>Nat. Commun., 2026</strong></i></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y"><span>https://doi.org/10.1038/s41467-026-73676-y</span></a><span>&nbsp;</span></p><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><h4><strong>Abstract</strong></h4><p>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</p></div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

<p><strong>Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.</strong></p><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity</strong></a><strong> 10.2 highlights</strong></h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4><strong>Instrument control modules</strong></h4><p>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"></p><h4><strong>MS data import/export improvements</strong></h4><p>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"></p><h4><strong>Obsolete hardware support terminated</strong></h4><p>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"></p><blockquote><p><strong>Explore the complete list of all changes, fixes, and additions in version 10.2.</strong></p><p><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1"><strong>See the complete changelog&nbsp;</strong></a></p></blockquote>

Clarity Chromatography Software 10.2 released!

<p><strong>🎤Dr. Leigh Terry (University of Alabama)</strong></p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.</strong></p><p>While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.</p><h4><strong>In this episode, we cover:</strong></h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted<strong> LC-MS/MS methods</strong></li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (<strong>CIC</strong>) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4><strong>Key Insight:</strong></h4><p>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.</p><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.</p><h4><strong>Understanding PFAS and Their Significance</strong></h4><h5><strong>The University of Alabama Water Quality Research Center</strong></h5><p>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.</p><p>The laboratory specializes in:</p><ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul><p>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.</p><h4><strong>Why Focus on PFAS?</strong></h4><p>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.</p><p>According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:</p><ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul><p>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.</p><h4><strong>The Development of Standardized PFAS Methods</strong></h4><p>One major advancement in PFAS analysis has been the development of standardized EPA methods.</p><p>These methods allow:</p><ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul><p>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.</p><h4><strong>How Large Is the PFAS Universe?</strong></h4><p>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.</p><p>PFOA and PFOS are often referred to as:</p><ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul><p>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.</p><p>Researchers estimate:</p><ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul><p>Scientific debate continues regarding classification and definition, making precise counting difficult.</p><h4><strong>Why PFAS Are Called "Forever Chemicals"</strong></h4><p>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.</p><p>PFAS persistence results from two key chemical properties.</p><h5><strong>1. Amphiphilic Structure</strong></h5><p>PFAS molecules contain:</p><ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul><p>This unusual combination allows PFAS to partition between:</p><ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul><p>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.</p><h5><strong>2. Extremely Strong Carbon–Fluorine Bonds</strong></h5><p>The carbon–fluorine bond is among the strongest bonds in organic chemistry.</p><p>As Dr. Terry explains:</p><ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul><p>These properties make PFAS extremely persistent in both environmental systems and biological organisms.</p><h4><strong>Toxicity, Regulations, and Environmental Behavior</strong></h4><h5><strong>Understanding PFAS Toxicity</strong></h5><p>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.</p><p>Dr. Terry explains that EPA regulations generally consider three factors:</p><ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul><p>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.</p><p>This creates important scientific questions:</p><ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul><p>These uncertainties continue to drive active research.</p><h4><strong>What Happens to PFAS in Wastewater Treatment Plants?</strong></h4><p>PFAS do not behave like many conventional contaminants.</p><p>Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:</p><ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul><p>As a result:</p><ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul><p>Their distribution depends on wastewater composition and treatment processes.</p><h4><strong>Regulatory Monitoring and Targeted LC-MS Analysis</strong></h4><p>Current EPA regulations focus on targeted analysis of specific PFAS compounds.</p><p>Examples include:</p><ul><li>PFOA</li><li>PFOS</li></ul><p>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.</p><p>Advantages of targeted LC-MS analysis include:</p><ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul><p>However, limitations remain:</p><ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul><p>As Dr. Terry notes, targeted analysis provides only part of the overall picture.</p><h4><strong>The Fluorine Mass Balance Problem</strong></h4><p>One of the most important concepts discussed in the interview is fluorine mass balance.</p><p>When PFAS degrade, fluorinated fragments may remain in the environment.</p><p>This creates a critical question:</p><p>Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?</p><p>To answer this, researchers perform fluorine mass balance calculations using:</p><ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul><p>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.</p><h4><strong>Advanced Analysis, Treatment Technologies, and Future Directions</strong></h4><h5><strong>Combining Targeted and Bulk Fluorine Measurements</strong></h5><p>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.</p><p>Approaches include:</p><ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul><p>In these methods:</p><ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul><p>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.</p><h4><strong>Surprising Findings from Combined Analysis</strong></h4><p>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.</p><p>Dr. Terry reports that:</p><ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul><p>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.</p><h4><strong>Preventing PFAS Contamination in the Laboratory</strong></h4><p>PFAS analysis requires exceptionally strict quality control.</p><p>Dr. Terry's laboratory routinely:</p><ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul><p>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.</p><h4><strong>Current PFAS Treatment Technologies</strong></h4><p>Three technologies currently dominate PFAS removal in drinking water treatment:</p><ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul><p>Selection depends on:</p><ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul><p>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.</p><h4><strong>Membrane Filtration and Pretreatment</strong></h4><p>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.</p><p>Her own work focuses on pretreatment through biological filtration.</p><p>The strategy involves:</p><ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul><p>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.</p><h4><strong>Regional Monitoring Projects</strong></h4><p>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.</p><p>Their observations indicate:</p><ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul><p>Examples include:</p><ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul><p>Source identification remains a critical component of environmental monitoring programs.</p><h4><strong>Future Directions for PFAS Monitoring</strong></h4><p>Looking ahead, Dr. Terry sees several opportunities for improvement.</p><p>Desired advances include:</p><ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul><p>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.</p><h4><strong>Dr. Terry's Perspective on Long-Term Solutions</strong></h4><p>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.</p><p>Rather than focusing solely on remediation, she emphasized prevention.</p><p>Her view is that society should:</p><ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul><p>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.</p><h4><strong>Final Thoughts</strong></h4><p>Dr. Terry encourages consumers to:</p><ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul><p>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.</p><h4><strong>Conclusion</strong></h4><p>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.</p><p>The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Waters ACQUITY Arc LC System

The ACQUITY™ Arc™ System is an all-in-one platform for HPLC and UHPLC, designed for robust method development and seamless transfer between modern LC systems, addressing a wide range of analytical challenges.

<p class="cke-user_style-important"><strong>Built upon a foundation of reliability</strong></p><p data-pm-slice="1 1 []"><strong>The method development process is changing. New regulations, software and analytical technologies are rapidly changing how methods are designed, transferred, and monitored over their lifecycle. An all-in-one platform for HPLC and UHPLC separations, the ACQUITY™ Arc™ System is designed to meet the spectrum of challenges you face from robust method development to seamless transfer between any modern LC systems.</strong></p><p data-pm-slice="1 1 []">With the <strong>ACQUITY Arc System</strong>, you can experience true plug-and-play method compatibility for HPLC and UHPLC separations. Significant productivity gains can be realized by deploying a single LC platform that allows for the efficient transfer, adjustment, and improvement of methods from any LC platform without compromise in method integrity.</p><figure class="image"><img style="aspect-ratio:768/886;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_63246061ea.jpg" alt="Waters ACQUITY Arc System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_63246061ea.jpg 136w," sizes="100vw" width="768" height="886"></figure><p data-pm-slice="1 1 []">Laboratory-driven organizations have relied on Waters for over 60 years to deliver reliable, robust, and reproducible solutions to ensure confidence in their analytical results. With over 100,000 LC systems installed, we understand the significant impact the right technology has on your business, and the implications it has on characterizing the quality and safety of your products.</p><p data-pm-slice="1 1 []">Methods with greater agility, robustness, and capacity for change can help you reduce regulatory burden, risk of failure and costs. Visit <a disabled="true">www.methods.waters.com</a> to learn more about how method life cycle management (MLCM) connects your method today to your method tomorrow.</p><h3><strong>Designed for discovery to routine QC applications</strong></h3><p>The <strong>ACQUITY Arc System</strong> is the only available LC system with regulatory compliant-ready hardware and software for both HPLC and UHPLC separations in one instrument. With unrivaled column capacity and Arc Multi-flow path™ technology, the<strong> ACQUITY Arc System</strong> allows you to generate new methods and modernize legacy HPLC methods faster than ever before.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_2_c06ba42baa.jpg" alt="Waters ACQUITY Arc System: The ACQUITY Arc System can replicate your established HPLC methods by compensating for these differences with unique features such as the Arc Multi-Flow Path technology and Gradient SmartStart. These innovative attributes enable seamless replication of your existing chromatographic results to meet your acceptance criteria without changes to validated gradient tables." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_2_c06ba42baa.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Comprehensive detector portfolio</strong></h4><p>High-performance analytical detectors designed to maximize HPLC and UHPLC performance, delivering exceptional sensitivity and linearity for your assays.</p><p>Photodiode array, UV/Vis, fluorescence, refractive index, evaporative light scattering, and mass detection, including the ACQUITY QDa™ Detector.</p><h4><strong>Gradient SmartStart</strong></h4><p>Automatically manage gradient start time and pre-injection steps to maximize sample throughput. Automatically counteract differences in system dwell volume without the need to alter gradient table inputs.</p><h4><strong>Quaternary solvent management</strong></h4><p>Precise and accurate blending of up to four solvents with automated solvent compressibility compensation. Increase solvent flexibility with an optional, integrated solvent select valve, providing access to six additional solvents.</p><h4><strong>Negligible carryover</strong></h4><p>Advanced flow-through needle design minimizes carryover by continuously cleansing the needle during run. User-configurable wash settings provide flexibility to address even the most complex sample matrices.</p><h4><strong>Thermal management options</strong></h4><p>Column manager options available for up to 15 columns ranging in length from 50 mm to 300 mm, with a temperature range of 4 °C to 90 °C. Integrated column selection valve options provide unattended, fast column changeover.</p><h4><strong>Auto•Blend™ Plus Technology</strong></h4><p>Program gradients directly in terms of pH and ionic strength to minimize manual mobile phase preparation, reduce potential for human error, and accelerate method robustness testing for chromatographic methods.</p><h4><strong>Arc Multi-flow path technology</strong></h4><p>Delivers plug-and-play method compatibility with HPLC or UHPLC methods with the flip of a switch.</p><p>Easily replicate or improve established methods by simply selecting between Path 1 (HPLC) or Path 2 (UHPLC) without any manual user intervention.</p><h4><strong>Column Technology</strong></h4><p>Industry-leading selectivity choices to meet the needs of virtually every LC application.</p><h4><strong>Laboratory Informatics</strong></h4><p>Software control within your existing Empower™ or MassLynx™ infrastructure.</p><h3><strong>Replicate your established methods</strong></h3><p data-pm-slice="1 1 []">The <strong>ACQUITY Arc System</strong>, enabled by unique Arc Multi-flow path technology, easily accepts and replicates methods from any HPLC platform, without compromise.&nbsp;</p><p data-pm-slice="1 1 []">Whether you are trying to replicate an out-of-date HPLC method, transfer methods across the organization, or to contract partners, the <strong>ACQUITY Arc System</strong> gives you ultimate flexibility to reproduce, modernize, and transfer existing methods with ease and greater cost efficiency, while preserving the integrity of your methods.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_3_c8c7901de3.jpg" alt="Waters ACQUITY Arc System: Analysis run on a Vendor X LC system and replicated on the ACQUITY Arc System. Conditions: 15 to 35% B in 3 min, 35 to 95% B in 2 min; Mobile phase A: 0.1% HCOOH in H 2 O, mobile phase B: 0.1% HCOOH in ACN; Flow rate = 2.0 mL/min; Column: XSelect™ CSH C 18 , 4.6 x 150 mm, 5 μm; Temp. = 30 °C; UV@ 260 nm; 10.0 μL injection volume." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_3_c8c7901de3.jpg 245w," sizes="100vw" width="1099" height="596"></p><p>Upgrade your laboratory with a versatile, modern LC system that delivers HPLC and UHPLC method compatibility at the flip of a switch.</p><h3><strong>Modernize your methods</strong></h3><p>The United States Pharmacopeia (USP) is championing pharmacopeial harmonization and modernization efforts, and international organizations are following suit. As a result, the USP is encouraging the modernization of older compendial methods to ensure they are fit for purpose.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_4_a1aaab8d02.jpg" alt="Waters ACQUITY Arc System: Quetiapine fumarate system suitability solution analyzed on the ACQUITY Arc System using HPLC, UHPLC, and UPLC™ column/method conditions for the quetiapine impurity method. Peak identification: (1) quetiapine related compound G, (2) quetiapine related compound B, (3) quetiapine desethoxy, and (4) quetiapine." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_4_a1aaab8d02.jpg 245w," sizes="100vw" width="1092" height="678"></p><p>Revisions to these guidelines give you more flexibility to modify your existing pharmacopeial methods with modern techniques and technologies, and in some cases avoid revalidation. The <strong>ACQUITY Arc System</strong>, featuring the Waters Columns Calculator, allows you to confidently scale your gradient methods from a 3.5 μm to a 2.5 μm particle, decreasing run times, lowering solvent consumption, and increasing sample throughput.</p><h3><span><strong>Save time. Reduce cost.</strong></span></h3><p data-pm-slice="1 1 []">With the right column, the <strong>ACQUITY Arc System</strong> is the ideal solution to accommodate methods from any LC platform.&nbsp;</p><p data-pm-slice="1 1 []">From concept to production, the transition your product takes through the product development cycle often requires rigorous LC assays to properly characterize and assess its readiness to advance to the next stage. These LC assays are often adapted to accommodate the technology available from one stage to the next, in some cases requiring an adjustment from a smaller particle size (sub-2-μm) to a larger particle size (2.5 μm–5 μm).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_5_d9dd95135c.jpg" alt="Waters ACQUITY Arc System: The ACQUITY Arc System enables the support of HPLC and UHPLC methods on a single platform." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_5_d9dd95135c.jpg 173w," sizes="100vw" width="959" height="860"></p><p>The <strong>ACQUITY Arc System</strong> provides the flexibility to maximize productivity through efficient and rapid 2.5 μm–2.7 μm UHPLC separations, or to accommodate larger 3.0 μm–5 μm HPLC particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_6_1ebc2c67e7.jpg" alt="Waters ACQUITY Arc System: Save time. Reduce cost." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_6_1ebc2c67e7.jpg 245w," sizes="100vw" width="1683" height="469"></p><h3><span><strong>Mitigate your risk</strong></span></h3><p data-pm-slice="1 1 []">With an expanded range of column manager options enabling up to 15-column switching from 4 °C to 90 °C, the <strong>ACQUITY Arc System</strong> will help you significantly reduce the time spent generating robust methods. Equipped with the plug-and-play ACQUITY QDa Mass Detector, the <strong>ACQUITY Arc System</strong> tracks peaks in your method and reveals hidden coelutions of similar analytes, ensuring you have complete confidence in the reliability and reproducibility of your method now and far into the future.</p><h4><strong>Thermal management options</strong></h4><p data-pm-slice="1 1 []">Active and passive pre-heating options available, with up to 15-column switching from 4 °C to 90 °C. Automated monitoring and tracking of column usage with eCord™ technology available with active pre-heating options.</p><h4><strong>Quaternary solvent management</strong></h4><p data-pm-slice="1 1 []">Accelerate your method development with precise and accurate blending of up to four solvents. Increase solvent flexibility with an optional solvent select valve, providing access to six additional solvents.</p><h4><strong>Auto•Blend Plus Technology</strong></h4><p data-pm-slice="1 1 []">Reduce human error and accelerate method robustness testing for chromatographic methods by programming gradients to control mobile phase pH and ionic strength.</p><h4><strong>Comprehensive detector portfolio</strong></h4><p>Diverse portfolio of detectors for method development, method transfer, and routine use.</p><h4><strong>ACQUITY QDa Detector</strong></h4><p>Mass detection built around the needs of analytical scientists for chromatographic analysis. Robust, reliable and requiring no adjustments, it easily integrates with your<strong> ACQUITY Arc System.</strong></p><h5><strong>Support</strong></h5><p data-start="0" data-end="175">Empower eLearning modules to help train your lab on Empower 3 and Method Validation. Applications support that’s customized based on your method development needs.</p><h5><strong>Consumables</strong></h5><p data-start="177" data-end="410" data-is-last-node="">Industry-leading selectivity choices to meet the needs of virtually every LC application. Quality Control (QC) Reference Material benchmarks your <strong>ACQUITY Arc System </strong>and enables perfect resolution. Certified clean vials.</p><h5><strong>Laboratory Informatics</strong></h5><p>Software control within your existing Empower or MassLynx infrastructure. Empower 3 Method Validation Manager allows you to perform chromatographic method validation in one application. Optional S-Matrix Fusion Software automates your method development according to AQbD guidelines.</p><h3><span><strong>Develop methods that stand the test of time</strong></span></h3><p>Need an orthogonal detection approach to UV in your method development? Streamline your method development with an LC-MS solution that’s as easy to use as photodiode array detection.</p><p>The <strong>ACQUITY Arc System</strong> is fully compatible with the ACQUITY Arc, supporting efficient and robust method development by expanding the information you can get from your system using the same Empower Software. The system automatically pairs qualitative mass spectral data to the peaks in your chromatogram, leaving nothing to chance.</p><ul><li>Confirm the identity of peaks in a separation without injecting standards.</li><li>Identify new peaks in a separation with molecular weight information.&nbsp;</li><li>Detect unresolved peaks and determine if a peak is homogeneously pure.</li><li>Easily track peaks as method conditions are changed.</li></ul><p data-start="272" data-end="600">Using Empower 3 Software and its Custom Calculations and Custom Reporting functions enables you to quickly and automatically evaluate chromatographic data. Configurable reports allow you to choose optimal conditions based on metrics rather than judgment, ensuring a comprehensive assessment of data and best method conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_7_e7ef959203.jpg" alt="Waters ACQUITY Arc System: Method transfer of USP Assay for Fluconazole using the ACQUITY Arc System. Here the peak has same m/z of a related compound. Conditions: Mobile phase: 20:80 acetonitrile/ water; Flow rate = 0.5 mL/min; Column: XSelect HSS T3, 4.6 x 150 mm, 3.5 μm; UV@ 260 nm; 20.0 μL injection volume." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_7_e7ef959203.jpg 224w," sizes="100vw" width="939" height="654"></p><h3><strong>Streamline your workflow</strong></h3><div><div data-message-author-role="assistant" data-message-id="7b732be9-0a45-45fb-b585-70a90c1a74d6" dir="auto" data-message-model-slug="gpt-4o"><div><div><p data-start="602" data-end="827">How would you benefit from increased assay consistency between analysts and from lab to lab? What would you gain by accelerating your robustness testing prior to transferring methods to QA/QC?</p><p data-start="829" data-end="1392" data-is-last-node="">Auto•Blend Plus Technology is a novel software tool that extends traditional quaternary solvent blending to permit gradient programming directly in units of pH and ionic strength, providing the ability to vary composition in even the smallest increments of pH (even those differing by 0.1 pH units or less).&nbsp;</p><p data-start="829" data-end="1392" data-is-last-node="">With Auto•Blend Plus, the cumbersome and error-prone approach of manually preparing several pre-mixed mobile phases can now be eliminated with on-demand mobile phase creation, providing significant time savings and improved productivity to the laboratory.</p></div></div></div></div>

Waters Alliance HPLC System

 With its proven performance history, the Alliance HPLC is the industry’s analytical HPLC system of choice, ideally suited for methods development and routine analysis.

<div><div><h4><strong>Reliably achieve quality, compliant data. Every time, in every lab.</strong></h4></div></div><div><div><p><strong>Liquid chromatography (LC) systems must deliver unequivocal results that give you confidence in your scientific and business decisions. Waters LC commitment continues to succeed with the latest Alliance HPLC System – trusted by scientists worldwide for the data to drive informed decisions.</strong></p><div><div><div><div><p>Building on <strong>industry-standard performance and reliability</strong>, the <strong>Alliance HPLC System</strong> provides the <strong>capabilities to meet today’s business challenges</strong>. With its <strong>proven performance history</strong>, the Alliance HPLC is the <strong>industry’s analytical HPLC system of choice</strong>, ideally suited for <strong>methods development and routine analysis</strong>.</p><ul><li>Time-tested, dependable, and robust workhorse for your HPLC separations</li><li>Integrated solvent and sample management with consistent system-to-system performance and high reproducibility</li><li>Simplify your daily LC system start-up with advanced software functionality</li></ul><h5><strong>Recommended Use</strong></h5><p>For standard HPLC workflows where <strong>consistency and compliance</strong> are the highest priorities.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_1_6b63ab6d62/Waters-Alliance-HPLC-System-1.jpg" alt="Waters Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_1_6b63ab6d62/thumbnail_Waters-Alliance-HPLC-System-1.jpg 147w," sizes="100vw" width="908" height="961"></p><div><div><h3><strong>Excellence through the years</strong></h3><p>The consistency of the Alliance HPLC System gives you <strong>exceptional confidence in your analytical results</strong>, day-to-day and year-to-year. Get <strong>accurate and reproducible results</strong> with <strong>Waters flagship HPLC technology</strong>, which provides <strong>consistent and reliable performance</strong>. Whether your Alliance HPLC System is newly manufactured, or you're working on one that is several years old, the Alliance HPLC System is a <strong>time-tested, dependable,</strong> and <strong>robust </strong>workhorse for your HPLC separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_dfafc334c1.jpg" alt="Waters Alliance HPLC System: Comparison of the separation of carbamates using two different Alliance HPLC Systems, manufactured 7 years apart. The results are equivalent." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_dfafc334c1.jpg 245w," sizes="100vw" width="1024" height="532"></p></div></div><div><div><h3><strong>Performance that exceeds your method requirements</strong></h3><p>The <strong>Alliance HPLC System</strong> ensures the <strong>reliability of your results</strong> via <strong>high injection precision</strong> and <strong>repeatable and accurate flow rates</strong>, resulting in <strong>easy peak identification</strong>, <strong>precise quantification</strong>, and <strong>exceptional retention time repeatability</strong>.</p><p>The integrated solvent delivery system features <strong>quaternary blending with a fixed delay volume</strong> for <strong>consistent and predictable results</strong>, regardless of system back pressure. The sample managers’ <strong>flexible injection capabilities</strong> cover a wide injection volume range (up to 2 mL) with <strong>excellent carryover performance</strong>, typically <strong>below 0.005%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_6dab43c365.jpg" alt="Waters Alliance HPLC System: USP method for Budesonide on the Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_6dab43c365.jpg 245w," sizes="100vw" width="1024" height="580"></p></div></div><div><div><h3><strong>Advancements in column chemistry and mass detection</strong></h3><p>The <strong>Alliance HPLC</strong> can leverage <strong>smaller particle size column chemistry</strong> to improve your laboratory's efficiency. With <strong>CORTECS 2.7 µm particle size columns</strong>, your lab will benefit from <strong>improvements in resolution</strong> and <strong>faster run times</strong> when transferring from a 5 µm size particle chemistry.</p><p>Further <strong>increase confidence in your data</strong> by adding <strong>mass confirmation with the ACQUITY QDa Detector</strong>, which is <strong>simple to use</strong> and can easily integrate into your existing workflow. <strong>Analysis in Empower 3 Software</strong> combines data from <strong>UV/Vis and mass spectrometry (MS)</strong> peaks to give <strong>mass information to each peak</strong> in the separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_3_3711671b2e.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_3_3711671b2e.jpg 245w," sizes="100vw" width="1024" height="550"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_4_bf40981882.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_4_bf40981882.jpg 240w," sizes="100vw" width="1024" height="666"></p></div></div><div><div><h3><strong>Features and benefits</strong></h3><ul><li><strong>Quaternary solvent blending. </strong>Support isocratic and gradient applications with 11 unique gradient profile curves and blend up to 4 solvents in any proportion with Auto-Blend Technology. Compatible with solvent types such as reversed-phase, normal-phase, GPC, SEC, and IEX.</li><li><strong>Advanced solvent delivery capabilities. </strong>Serial flow path with pulse-free solvent delivery without the use of dampeners. Online solvent degassing and automated solvent compressibility compensation for accurate flow. Integrated seal wash for robust operation with all solvent types is standard.</li><li><strong>Optional, temperature-controlled environment.</strong> House up to 120 vials in an optional, temperature-controlled environment. Achieve low carryover with active and user programmable needle wash. Variable injection volumes from 0.1 to 100 μL standard, and up to 2 mL optional. Support STAT samples and auto-addition.</li><li><strong>Detection and column options. </strong>Get optional column heating, heating/cooling, or column switching capabilities. Detection capabilities include photodiode array, UV/Vis, fluorescence (FLR), refractive index (RI), evaporative light scattering (ELS), electrochemical, conductivity, and mass detection.</li><li><strong>Intuitive interface and software. </strong>Get instant access to system settings through the large LCD screen with simplified daily set-up with SystemPREP. Supports Empower, MassLynx, and 3<sup>rd</sup> party software packages.</li><li><strong>Compliance-ready. </strong>Supports Waters Empower-based automated system qualification tool (SQT), minimizing the time and cost of annual qualification with standards like GMP, GLP, ISO 17025, and USP &lt;1058&gt;.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_47fe8951d2/Waters-Alliance-HPLC-System-2.jpg" alt="Waters Alliance HPLC System: Alliance HPLC System provides powerful functionality for all your application needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_47fe8951d2/thumbnail_Waters-Alliance-HPLC-System-2.jpg 161w," sizes="100vw" width="903" height="874"></p><h3><strong>Specifications</strong></h3><ul><li><strong>Maximum operating pressure</strong>: 5,000 psi</li><li><strong>Front panel display</strong>: Easy to use control panel</li><li><strong>Mobile phases</strong>: Up to 4 with Auto-Blend Technology</li><li><strong>Flow rate range</strong>: Up to 10 mL/min</li><li><strong>Sample carryover</strong>: ≤0.0025% (Caffeine) under specified conditions</li><li><strong>Sample compartment</strong>: 4–40 °C</li><li><strong>Pump options</strong>: Quaternary</li><li><strong>Solvent conditioning</strong>: Vacuum Degas</li><li><strong>Column capacity</strong>: Standard up to 3 columns</li><li><strong>Maximum sample capacity</strong>: 120</li><li><strong>Detector options</strong>:&nbsp;<ul><li>Tunable UV</li><li>Photo Diode Array</li><li>Fluorescence</li><li>Refractive Index</li><li>Evaporative Light Scattering</li><li>Electrochemical</li><li>Conductivity</li><li>Mass Spectrometry</li></ul></li><li><strong>External control</strong>:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Empower Software</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635">MassLynx Software</a></li></ul></li><li><strong>Physical specifications</strong>:<ul><li><strong>Width:</strong> 45.7 cm (18.0 in.) or 58.4 cm (23.0 in.) with column heater</li><li><strong>Height:</strong> 57.1 cm (22.5 in.)</li><li><strong>Depth:</strong> 57.1 cm (22.5 in.) or 64.8 cm (25.5 in.) with sample heater/cooler</li></ul></li></ul></div></div></div></div></div></div></div></div>

Waters Alliance iS Bio HPLC System

Alliance iS Bio HPLC combines MaxPeak HPS Technology, bio-inert separations, reduced errors, and enhanced reproducibility for biopharma QC workflows.

<p><strong>The Waters Alliance iS Bio HPLC System is an advanced liquid chromatography platform specifically designed for biopharmaceutical quality control laboratories. Combining exceptional bio-inertness, intuitive operation, and intelligent automation tools, the system helps reduce human error, improve productivity, and ensure consistent analytical performance.</strong></p><p>Featuring <strong>MaxPeak™ High Performance Surfaces (HPS) Technology</strong>, the Alliance iS Bio minimizes unwanted interactions between analytes and metallic flow path surfaces. This improves analyte recovery, sensitivity, reproducibility, and retention time consistency while reducing system conditioning requirements. The technology is particularly beneficial for oligonucleotides, phosphopeptides, monoclonal antibodies, and other metal-sensitive biomolecules.</p><p>The system integrates intuitive touchscreen operation, guided workflows, RFID-enabled column tracking, and advanced compliance-support tools. Combined with corrosion-resistant construction materials and robust performance under challenging operating conditions, the Alliance iS Bio HPLC System is an ideal platform for modern biopharma QC laboratories.</p><h3><strong>Key Features</strong></h3><ul><li>MaxPeak™ High Performance Surfaces (<strong>HPS) Technology</strong></li><li>Highly <strong>bio-inert sample flow path</strong></li><li>Reduced analyte-surface interactions</li><li>Improved analyte recovery and sensitivity</li><li>Enhanced reproducibility and retention time consistency</li><li>Up to 40% reduction in human errors</li><li>Intuitive touchscreen operation</li><li>Guided workflows</li><li><strong>RFID-enabled eConnect column</strong> tracking</li><li><strong>Intelligent Method Translator App</strong> (iMTA)</li><li>Automated column history tracking</li><li>Empower Audit Trail functionality</li><li><strong>waters_connect™</strong> System Monitoring</li><li>Integrated maintenance and troubleshooting tools</li><li><strong>Carryover ≤ 0.002%</strong></li><li>Robust operation across extreme temperature conditions</li><li>Tool-free finger-tight fittings</li></ul><h3><strong>Technical Specifications</strong></h3><h4><strong>Chromatography System</strong></h4><ul><li><strong>System type: </strong>HPLC</li><li><strong>Pump configuration: </strong>Quaternary</li><li><strong>Injection type:</strong> Flow-through needle</li><li><strong>Solvent conditioning:</strong> Passive pre-heating</li></ul><h4><strong>Operating Parameters</strong></h4><ul><li><strong>Maximum operating pressure:</strong> 12,000 psi</li><li><strong>Flow rate range:</strong> 0.001–10.000 mL/min</li><li><strong>pH range:</strong> 1–13</li><li><strong>Carryover:</strong> ≤ 0.002% (caffeine, UV conditions)</li></ul><h4><strong>Sample Management</strong></h4><ul><li><strong>Sample compartment temperature:</strong> 4–40 °C</li><li><strong>Maximum sample capacity:</strong><ul><li>48-position 2.0 mL vial holder</li><li>54-position 2.0 mL vial holder</li><li>96-well plate</li><li>384-well plate</li><li>48-position 0.65 mL microcentrifuge tube plate</li><li>24-position 1.5 mL microcentrifuge tube plate</li></ul></li></ul><h4><strong>Column Management</strong></h4><ul><li>RFID column tracking</li><li>eConnect Waters Column support</li><li><strong>Capacity:</strong><ul><li>One column up to 300 mm length</li><li>Up to 8 mm internal diameter</li></ul></li></ul><h4><strong>Detector Options</strong></h4><ul><li>PDA Detector</li><li>TUV Detector</li></ul><h4><strong>Software and Informatics</strong></h4><ul><li>Empower™ Chromatography Data System (CDS)</li><li>Intelligent Method Translator App (iMTA)</li><li>waters_connect™ Monitoring</li><li>Audit Trail functionality</li><li>Automated column history tracking</li></ul><h4><strong>Construction</strong></h4><ul><li>Inert sample flow path with MaxPeak HPS Technology</li><li>Corrosion-resistant bio-compatible materials</li><li>High-salt tolerant design</li></ul><h4><strong>Dimensions and Weight</strong></h4><ul><li><strong>Width: </strong>49.9 cm</li><li><strong>Height: </strong>74.2 cm</li><li><strong>Depth:</strong> 63.5 cm</li><li><strong>Weight:</strong> 72.5 kg</li></ul><h4><strong>Typical Applications</strong></h4><ul><li>Biopharmaceutical QC</li><li>Monoclonal antibodies (mAbs)</li><li>Oligonucleotide analysis</li><li>Phosphopeptide analysis</li><li>Peptide mapping</li><li>Size exclusion chromatography (SEC)</li><li>Bioseparations</li><li>Pharmaceutical QC</li><li>Regulatory testing laboratories</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SEC_separation_of_a_USP_m_Ab_System_Suitability_Solution_on_a_legacy_HPLC_system_and_the_Alliance_i_S_Bio_HPLC_System_with_a_Bio_Suite_Diol_SEC_Column_851dc3a90f.jpg" alt="Waters: SEC separation of a USP mAb System Suitability Solution on a legacy HPLC system and the Alliance iS Bio HPLC System with a BioSuite Diol SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SEC_separation_of_a_USP_m_Ab_System_Suitability_Solution_on_a_legacy_HPLC_system_and_the_Alliance_i_S_Bio_HPLC_System_with_a_Bio_Suite_Diol_SEC_Column_851dc3a90f.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters Alliance iS HPLC System

Waters™ delivers a reliable partner for QC labs. The Alliance™ iS HPLC System enhances efficiency, reduces errors, and simplifies workflows - your new essential lab ally.

<h3><strong>The Alliance iS HPLC System didn’t start in our lab.</strong></h3><p class="cke-user_style-important"><strong>It started in yours.</strong></p><p><strong>Pharmaceutical Quality Control (QC)</strong> labs face relentless demand to <strong>do more with less</strong> amid higher staff turnover, growing levels of sample complexity, and the drive to reduce errors.</p><p>With regulatory scrutiny and faster time to market fueling these intense demands, the need for <strong>simplicity has never been greater</strong>.</p><p>Waters™ understands the challenges you face and is ready to deliver a true ally for your laboratory. With capabilities developed specifically for the QC laboratory, the <strong>Alliance™ iS HPLC System</strong> will quickly become your favorite ally.</p><ul style="list-style-type:disc;"><li>Intuitively prevents up to 40% of common errors*</li><li>Provides fast, simple guidance at the point of need</li><li>Boosts productivity and capacity through effective resource utilization and error-free operation</li><li>Drives workflow efficiencies and quality improvements</li><li>Improves data integrity</li></ul><p>*Based on Waters’ market research in 2022, surveying 56 global Pharma QC labs running &gt;25 systems</p><h3><strong>Common challenges facing the QC lab</strong></h3><p class="cke-user_style-important"><strong>What you are telling us</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg" alt="Waters Alliance iS HPLC System - What you are telling us" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg 245w," sizes="100vw" width="2038" height="1132"></p><h3><strong>Meet your new lab ally</strong></h3><p>Intuitive and reliable, the <strong>Alliance iS HPLC System</strong> is designed to work seamlessly with <strong>Empower™ Software</strong> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720007883en_41b7055d98/720007883en.pdf"><strong>eConnect™ Column Tag Technology</strong></a>, unlocking unique features and functionality that eliminate the need to choose between simplicity, efficiency, and quality.</p><h3><strong>Step-change the performance of your lab</strong></h3><p class="cke-user_style-important"><strong>Achieve First Time Quality (FTQ) with the Alliance iS HPLC System</strong></p><p>The <strong>Alliance iS HPLC System</strong> supports FTQ by reducing the risk of common errors and improving the reliability of analytical measurements. Your lab can <strong>reduce reagents and consumables waste</strong>, and automated system checks help to meet critical error reduction KPIs. The increase in operational efficiency and productivity will save time and money and boost the capacity of your lab.**</p><p>With ease of use at the heart of the user experience, the <strong>Alliance iS HPLC System</strong> identifies potential errors earlier, reducing the number of retests and failure investigations. This supports a Quality culture resulting not only in safer products critical to human health and well-being, but also an improved working environment, directly impacting employee retention and motivation.**</p><p>**vs the Alliance HPLC System</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg" alt="Waters Alliance iS HPLC System with bottles connected." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>The System: intuitively simple</strong></h3><h5><strong>System Status Lighting and Solvent Tubing Clips&nbsp;</strong></h5><p>Status lighting provides you with a clear visual indication of the system status (in use, idle etc), while the color-coded solvent tubing clips ensure the tubing remains organized to enable tracing from instrument to the solvent bottle flags.</p><h5><strong>Made for Empower</strong></h5><p><strong>Waters Empower Software Suite</strong> helps your entire laboratory operate better, with the industry’s most advanced data acquisition, management, processing, analytics, and reporting capabilities. Empower Software provides you an environment where your records are traceable, so you always have full control of your most critical asset - your data.</p><h5><strong>waters_connect™ System Monitoring&nbsp;</strong></h5><p>Maximize lab productivity - utilize resources more effectively with real-time lab management from anywhere, anytime. System Monitoring provides intuitive and centralized access to detailed Empower Software instrument information via the waters_connect Cloud Platform, without compromising security or compliance.</p><h5><strong>Intelligent Method Translator App&nbsp;</strong></h5><p>Working with Empower Software, the iMTA enables you to create an Alliance iS HPLC System Instrument Method directly from a Waters or other third-party LC system. Basic chromatographic conditions are automatically transferred for you, and the new Empower instrument method can be reported, providing traceability to the original method for compliance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg" alt="Waters Alliance iS HPLC System with bottles connected and the doors open." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Say ‘hi’ to your lab ally</strong></h3><h5><strong>Intuitive touchscreen&nbsp;</strong></h5><p>An easy-to-use, advanced touchscreen integrates with Empower Software and provides easy-to-follow steps. Pre-run checks for common errors such as missing vials, incorrect column, and qualification/preventative maintenance requirements enable you to catch these mistakes before they result in a sample analysis failure, preventing re-runs.</p><h5><strong>Help at your fingertips&nbsp;</strong></h5><p>The Alliance iS HPLC System support journey delivers smart on-board technology that digitally brings you straight to the information you need at the new Waters Help Center. Train and troubleshoot with ease, knowing that Waters will support you at every stage.</p><h5><strong>Smart column capabilities&nbsp;</strong></h5><p>Ensure complete column traceability and facilitate post-run troubleshooting with the eConnect HPLC Columns. Ready-to-use from the box, eConnect columns are securely fitted with an NFC-enabled eConnect device tag, ensuring they are always automatically identified, verified, and tracked by the system. These second-generation smart columns propel today’s QC laboratory toward an entirely digital platform and away from manual logbooks.</p><h3><strong>Enjoy stress-free analysis</strong></h3><p>The Alliance iS HPLC System keeps you and your lab stress-free and running smoothly with an intuitive touchscreen that supports the productivity and efficiency of your lab.</p><ul><li>Start your day easily with a system guided start-up.</li><li>Pre-run checks to minimize sample analysis failures due to human error.</li><li>When issues arise, Alliance iS HPLC System provides you with information about what needs attention.</li><li>Guided workflows provide step-by-step guidance on how to resolve common issues.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg" alt="Waters Alliance iS HPLC System - Enjoy stress-free analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg 204w," sizes="100vw" width="1388" height="1063"></p><h3><strong>Built to perform, time after time</strong></h3><h4><strong>Intuitive innovation</strong></h4><p>Developed with the singular goal of providing innovation that solves the problems that matter in the QC lab, the Alliance iS HPLC System includes a range of new and unique features.&nbsp;</p><ul><li>New electronics to enable the Alliance iS HPLC System smart features (including advanced touchscreen, eConnect Column Tag Technology, and unparalleled interactivity with Empower Software)</li><li>Redesigned check valves and mixer for increased performance and robustness</li><li>Faster detector warm-up time and reduced drift to ensure you’re up and running quickly, for as long as your analysis requires</li><li>Robust performance when operating at the extremes of the Column Heater Cooler and Sample Manager temperature ranges</li><li>Class leading carryover performance and injection precision</li></ul><figure class="image"><img style="aspect-ratio:1561/740;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg" alt="Waters Alliance iS HPLC System - Overlay of 42 injections of gradient performance mix over 44 hours demonstrated RT repeatability of 0.5% RSD on the Alliance iS HPLC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg 245w," sizes="100vw" width="1561" height="740"></figure><h3><strong>Alliance iS HPLC System gives you so much more</strong></h3><p class="cke-user_style-important"><strong>Unlock new potential with Empower Software</strong></p><p><strong>The Alliance iS HPLC System works exclusively with our </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008057en_af76145fe7/720008057en.pdf"><strong>Empower Software</strong></a><strong> version 3 Feature Release 4 </strong>or later, with fully enabled and differentiated features, starting with eConnect Technology. Empower Software elevates your quality, your business, and your lab.</p><ul style="list-style-type:disc;"><li>Intuitive method matching</li><li>Bi-directional touchscreen interactivity</li><li>Column tracking and history</li><li>Enhanced instrument traceability</li><li>Pre-run checks</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg" alt="Waters Alliance iS HPLC System gives you so much more." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg 245w," sizes="100vw" width="1385" height="674"></p><h3><strong>Help at your fingertips</strong></h3><p class="cke-user_style-important"><strong>From onscreen to onsite, we’re there for you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg" alt="Waters Alliance iS HPLC System - From onscreen to onsite, we’re there for you." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg 245w," sizes="100vw" width="1550" height="910"></p><p>Maintain peak performance, minimize unexpected downtime, and ensure regulatory compliance of your AllianceTM iS HPLC System by partnering with Waters Service. Waters FlexCHOICETM Full-Service Plan for Regulated Laboratories includes:</p><ul style="list-style-type:disc;"><li>Yearly Waters Performance Maintenance</li><li>Priority* remote and onsite service including all parts, labor and travel</li><li>Full suite of compliance services</li><li>Discounts on future hardware upgrades and user training</li><li>Optional coverage available:<ul><li>Additional Performance Maintenance visits</li><li>Two business day on-site response time**</li></ul></li></ul><p>Priority over non-contract customers **May not be available in all areas</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg" alt="Waters Alliance iS HPLC System - The first system designed for Empower, the Alliance iS HPLC System features intuitive simplicity built specifically for QC laboratories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg 234w," sizes="100vw" width="1024" height="682"></p>

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