Waters Alliance iS Bio HPLC System

The Waters Alliance iS Bio HPLC System is an advanced liquid chromatography platform specifically designed for biopharmaceutical quality control laboratories. Combining exceptional bio-inertness, intuitive operation, and intelligent automation tools, the system helps reduce human error, improve productivity, and ensure consistent analytical performance.

Featuring MaxPeak™ High Performance Surfaces (HPS) Technology, the Alliance iS Bio minimizes unwanted interactions between analytes and metallic flow path surfaces. This improves analyte recovery, sensitivity, reproducibility, and retention time consistency while reducing system conditioning requirements. The technology is particularly beneficial for oligonucleotides, phosphopeptides, monoclonal antibodies, and other metal-sensitive biomolecules.

The system integrates intuitive touchscreen operation, guided workflows, RFID-enabled column tracking, and advanced compliance-support tools. Combined with corrosion-resistant construction materials and robust performance under challenging operating conditions, the Alliance iS Bio HPLC System is an ideal platform for modern biopharma QC laboratories.

Key Features

• MaxPeak™ High Performance Surfaces (HPS) Technology
• Highly bio-inert sample flow path
• Reduced analyte-surface interactions
• Improved analyte recovery and sensitivity
• Enhanced reproducibility and retention time consistency
• Up to 40% reduction in human errors
• Intuitive touchscreen operation
• Guided workflows
• RFID-enabled eConnect column tracking
• Intelligent Method Translator App (iMTA)
• Automated column history tracking
• Empower Audit Trail functionality
• waters_connect™ System Monitoring
• Integrated maintenance and troubleshooting tools
• Carryover ≤ 0.002%
• Robust operation across extreme temperature conditions
• Tool-free finger-tight fittings

Technical Specifications

Chromatography System

• System type: HPLC
• Pump configuration: Quaternary
• Injection type: Flow-through needle
• Solvent conditioning: Passive pre-heating

Operating Parameters

• Maximum operating pressure: 12,000 psi
• Flow rate range: 0.001–10.000 mL/min
• pH range: 1–13
• Carryover: ≤ 0.002% (caffeine, UV conditions)

Sample Management

• Sample compartment temperature: 4–40 °C
• Maximum sample capacity:
  • 48-position 2.0 mL vial holder
  • 54-position 2.0 mL vial holder
  • 96-well plate
  • 384-well plate
  • 48-position 0.65 mL microcentrifuge tube plate
  • 24-position 1.5 mL microcentrifuge tube plate

Column Management

• RFID column tracking
• eConnect Waters Column support
• Capacity:
  • One column up to 300 mm length
  • Up to 8 mm internal diameter

Detector Options

• PDA Detector
• TUV Detector

Software and Informatics

• Empower™ Chromatography Data System (CDS)
• Intelligent Method Translator App (iMTA)
• waters_connect™ Monitoring
• Audit Trail functionality
• Automated column history tracking

Construction

• Inert sample flow path with MaxPeak HPS Technology
• Corrosion-resistant bio-compatible materials
• High-salt tolerant design

Dimensions and Weight

• Width: 49.9 cm
• Height: 74.2 cm
• Depth: 63.5 cm
• Weight: 72.5 kg

Typical Applications

• Biopharmaceutical QC
• Monoclonal antibodies (mAbs)
• Oligonucleotide analysis
• Phosphopeptide analysis
• Peptide mapping
• Size exclusion chromatography (SEC)
• Bioseparations
• Pharmaceutical QC
• Regulatory testing laboratories

Waters: SEC separation of a USP mAb System Suitability Solution on a legacy HPLC system and the Alliance iS Bio HPLC System with a BioSuite Diol SEC Column.