<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=136&amp;manufacturerItems=50&amp;page=2">Solid phase extraction</a> is routinely selected as a sample preparation technique prior to HPLC and GC analysis. It provides:<ul><li>Clean-up;</li><li>Analyte pre-concentration;</li><li>Solvent switching.</li></ul>This works well for any homogeneous liquid samples, but clearly a solid sample cannot be applied directly to an SPE device. Here we will consider how best to approach the handling of solid samples prior to SPE.The first stage of sample pre-treatment involves homogenising the solid sample with a suitable solvent. The solvent selected for this purpose will:<ul><li>Be effective at solubilising the analyte.</li><li>Be effective at solubilising the sample matrix, although this is not necessary providing the sample is blended well with the solvent.</li><li>Ideally it will be a weak solvent when it comes to the SPE stage. This may not be possible, and it may be necessary to dry down and reconstitute the sample prior to loading on to the SPE cartridge.</li></ul>Having formed a suspension of the sample in a suitable solvent the sample should be vortexed to promote thorough mixing, and then centrifuged to pellet the insoluble components of the matrix. The solvent should then be removed for further treatment.At this point the SPE mechanism needs to be selected:&nbsp;<h3>Reversed Phase SPE Method</h3>Mechanism / Weak Solvent / Strong Solvent<ul><li>Reversed phase – Water/buffer – Acetonitrile/Methanol (hexane strongest)</li><li>Normal phase – Hexane/Heptane – Mid polar organic such as DCM, MTBE, IPA (strongest is water)</li><li>Ion exchange – Aqueous buffer at a pH which promotes ion exchange interactions between analyte and SPE sorbent – Organic solvent, typically methanol with pH adjustment to disrupt the ion exchange interactions.</li></ul>Analyte properties and sample diluent both need to be considered at this point. Reversed phase extractions are effective for moderately polar to moderately hydrophobic analytes, but only if they are dissolved in a weak solvent such as water. If the sample is dissolved in a mid-polar solvent the sample will either need to be diluted, or alternatively dried down and reconstituted. The same is true for normal phase, where samples need to be dissolved in hexane/heptane for analytes to be successfully retained during sample loading onto the SPE device.These examples will hopefully illustrate this concept.<h4>The extraction of PCBs from soil.</h4>PCBs are hydrophobic and will dissolve readily in a non-polar solvent. Soil can be dried, weighed and then extracted using hexane (or heptane). Once thoroughly mixed and centrifuged the resulting solution can be applied directed to a conditioned silica SPE tube allowing for a normal phase extraction to be conducted.<h4>Tylosin from meat.</h4>Tylosin is an antibiotic, with a log P of 1.0. Extracting from meat with a mixture of acetonitrile and water (80:20) will break any hydrophobic interactions releasing the tylosin. After centrifugation the resulting liquid sample will require further dilution with water to reduce the loading solvent strength prior to a reversed phase extraction.<h4>Enilconazole from oranges.</h4>Enilconazole is a fungicide widely used in agriculture and is often used with citrus fruits. It can readily be extracted from oranges using acetonitrile. As enilconazole is basic, the addition of HCl to the acetonitrile extract will promote protonation of the analyte, allowing for it to be directly retained on a strong cation exchange SPE material. This greatly simplifies the extraction process as no dilution of the sample is required (in contrast to reversed phase, or normal phase extraction where 100% acetonitrile would cause the analyte to be unretained during loading).

Considerations when working with solid samples and SPE

ALS recognizes that Climate Change is one of the defining issues of our time and we strive to make Impact through Action in the areas of Sustainability, linked to the ALS core value of Caring.ALS intends to make the world a better place through innovation, science, and commitment to best practice. This year the ALS Board has signed off on a company commitment to Net Zero by 2050 as summarized on our <a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.com/en/sustainability?utm_source=email-sig-link-ca&amp;utm_medium=email&amp;utm_campaign=sustainability-report-23">annual sustainability report</a>. ALS is pleased to share our milestones and targets with clients.<h3>Background</h3>In 2021, ALS developed a climate change strategy, setting a target of 40% reduction in scope 1 &amp; 2 emissions by 2030. By end of FY23 (March 2023), ALS had achieved a 58% reduction, and achieved carbon neutrality for scope 1 &amp; 2 emissions – well ahead of plan. In the same month, ALS recorded a key milestone with the ALS Board approving our roadmap to reach Net Zero by 2050 (shared at right), with milestones noted and completed actions shaded green.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_b157904cab.jpg" alt="ALS Czech Republic: EnviroMail™12/Europe: ALS Net Zero Commitments &amp; Roadmap in Support of our Staff, Clients, Communities &amp; Shareholders." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_b157904cab.jpg 112w," sizes="100vw" width="805" height="1120"><h3>Further detail</h3>Key projects to date have included purchasing Renewable Electricity (RE) from the grid (we utilize greater than 90% RE across all operations), installing &gt;1.8MW of solar photovoltaic (PV) systems across 16 site locations, and continued implementation of our ALS Green Building Standards, which focus on HVAC systems, building insulation, LED lights, energy efficient plant and equipment, moving to electric vehicles (EVs), and staff engagement on adopting climate friendly practices to reduce actual energy demand.This ALS Net Zero commitment will involve reducing absolute scope 1 and 2 emissions by 95% and scope 3 emissions by 90% by 2050, against a 2020 baseline year. This is a challenging task for laboratory operations given requirements for chemical, plastic, and glassware usage, and significant air heating and cooling requirements due to fume hoods which cause frequent cycling of laboratory air.The ALS Roadmap includes a near-term target to reduce absolute scope 1 and 2 greenhouse gas emissions by 78% by 2030 against a 2020 baseline year.Over the next 2 years ALS will measure our scope 3 greenhouse gas inventory to confirm the calculated scope 3 estimate completed in FY2023 and will develop action plans for freight, purchased goods/services, wastes, and employee commutes. A near-term target for 2030 for scope 3 will then be set.Priority actions over the next 5 years will include:<ul><li>purchasing renewable electricity for &gt;95% of our operations</li><li>installing further solar energy PV systems (7 more installed YTD for FY24 with a further 9 systems to be completed in early FY25)</li><li>converting gas ovens and furnaces to electric where possible</li><li>selection of more energy-efficient vehicles; 30% of fleet to be hybrids and EVs by 2030</li><li>upgrading to ALS green building guidelines for new laboratory developments and refurbishments (better insulation, more efficient HVAC, solar PV systems, and upgraded building management systems)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_2_91b5e7ebf8.jpg" alt="ALS Czech Republic: EnviroMail™12/Europe: ALS Net Zero Commitments &amp; Roadmap in Support of our Staff, Clients, Communities &amp; Shareholders." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviro_Mail_12_Europe_ALS_Net_Zero_Commitments_and_Roadmap_in_Support_of_our_Staff_Clients_Communities_and_Shareholders_2_91b5e7ebf8.jpg 156w," sizes="100vw" width="1089" height="1089"><blockquote>We are pleased to share our commitments with our stakeholders. Please feel free to contact your local ALS representative for further information.</blockquote>

EnviroMail™12/Europe: ALS Net Zero Commitments & Roadmap in Support of our Staff, Clients, Communities & Shareholders

Solid-phase extraction (SPE) is one of the key techniques in modern analytical chemistry for pre-concentrating analytes prior to chromatographic analysis. However, traditional manual SPE procedures are often time-consuming, error-prone, and difficult to reproduce. In response to laboratories’ increasing demand for automation and efficiency, <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Watrex Praha</a> introduces the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> – an advanced system for online SPE.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Ole_uvod_oprava_bd77e4d8c2.jpg" alt="Watrex: Streamline™ OLE 200" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Ole_uvod_oprava_bd77e4d8c2.jpg 245w," sizes="100vw" width="1599" height="900"><h4>Comprehensive Automation of SPE&nbsp;</h4>The <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> is a fully automated solid-phase extraction unit designed for direct integration into HPLC systems. It enables efficient pre-concentration of up to eight different samples, minimizes the need for manual handling, and significantly boosts laboratory productivity.<h4>Advantages of SPE Automation with OLE 200&nbsp;</h4><ul><li>Increased laboratory throughput: Automatic processing of up to 8 samples without the need for operator intervention.&nbsp;</li><li>Reduced manual errors and improved result reproducibility.&nbsp;</li><li>Flexible integration: Compatible with any HPLC system, allowing implementation across various laboratory settings.&nbsp;</li><li>Time savings: Eliminates the need to manually transfer samples between the SPE and HPLC steps.&nbsp;</li><li>Multimodal selection of SPE cartridges based on analytes and matrix complexity.&nbsp;</li></ul><h4>Technical Specifications and System Design&nbsp;</h4>At the heart of the system is a high-pressure pump, ensuring precise dosing of both samples and solvents. The system’s design features three key valves:&nbsp;<ul><li>A 10-port selection valve for sample and wash solvent selection.&nbsp;</li><li>A high-pressure SPE cartridge selector, allowing switching between up to five different SPE cartridges.&nbsp;</li><li>A standard injection valve, connecting the device to any HPLC system.</li></ul>This setup ensures that each sample is precisely processed: drawn into an SPE cartridge, washed with one or two eluents, and then eluted directly into the chromatograph.&nbsp;<h4>Functional Diagram&nbsp;</h4><ol><li>The sample is drawn by the pump through the 10-port valve to the selected SPE cartridge.&nbsp;</li><li>The cartridge is washed with one or two eluents to remove interferences.&nbsp;</li><li>Analytes are then eluted with a selected solvent and delivered directly into the HPLC system via the injection valve.&nbsp;</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_OLE_clanek_obr_2_36588f90b9.jpg" alt="Watrex: Fig. 2 - Streamline™ OLE 200 Schematic" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_OLE_clanek_obr_2_36588f90b9.jpg 245w," sizes="100vw" width="1700" height="957"><h4>Efficiency, Reproducibility, and Flexibility&nbsp;</h4>Automation of the entire SPE process leads to greater reproducibility and reduces the risk of contamination or human error. The system allows sequential processing of multiple samples, each of which can be analyzed following a specific protocol. Thanks to its open design, the OLE 200 is compatible with any HPLC system, making it easy to integrate into existing lab workflows without major modifications.&nbsp;<h4>Application Potential&nbsp;</h4>The OLE 200 system is particularly suitable for:&nbsp;<ul><li>Pesticide and pharmaceutical residue analysis in water&nbsp;</li><li>Clinical and pharmaceutical analysis of biological fluids&nbsp;</li><li>Food safety and environmental monitoring&nbsp;</li><li>Forensic and toxicological analyses&nbsp;</li></ul>Thanks to its modular design and the ability to select various SPE cartridges based on polarity and interactions, the device can be easily adapted to specific applications.&nbsp;<h4>Operation and Software&nbsp;</h4>The system's user-friendly interface enables easy setup of each SPE step, including cartridge selection, volumes, elution solvents, and wash sequences. The entire process can be fully programmed, minimizing the need for operator involvement during analysis.&nbsp;<h4>Conclusion&nbsp;</h4>The <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/streamline-ole-200/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Streamline OLE 200</a> from <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_clanky">Watrex Praha</a> prepresents a modern and powerful tool for automated sample pre-concentration that significantly enhances efficiency, reliability, and accuracy in analytical laboratories. Its flexibility, robust design, and easy integration with existing systems make it an ideal solution for demanding applications in environmental analysis, pharmaceutical research, and routine quality control.&nbsp;<blockquote>Want to learn more? Visit our website at <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?utm_source=linkedin&amp;utm_medium=propagace&amp;utm_campaign=linkedin_clanky">www.watrex.com</a> or email us at watrex@watrex.com.</blockquote>

Flawless and Effortless: How the Streamline™ OLE 200 Transforms Sample Preparation for HPLC? 

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For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

ALS Czech Republic

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

WATREX Prague is the distributor and producer of HPLC techniques in the Czech Republic and Slovakia. Main Chromatografic and Related Techniques Supported: LC, GPC, IC, IEC, Automated SPE, LC Columns and special column packings, LC method development, Software development, Laboratory Robots.

Watrex Praha

Waters ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards Kits

Eliminate manual preparation of custom standard calibration mixes with ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards.

<div><div><h3>Frequent calibration for greater confidence in your results</h3></div></div><div><div>Eliminate manual preparation of custom standard calibration mixes with ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards, which provide the right number of data points for your targeted molecular weight (MW) range. ACQUITY APC Calibration Standards are specifically designed to match the molecular weight range of the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1950">ACQUITY APC Columns</a>.ACQUITY APC polymethylmethacrylate and polystyrene standards are configured into Low, Middle, and High Molecular Weight Calibration Kits, as well as a convenient Method Development Kit that includes the full separation range of all three kits combined. These chromatography standards are designed to provide a maximum range of narrow standard data points for a specific column set or molar mass range.With ACQUITY APC Calibration Standards, you can conduct calibration with 3-5x shorter cycle times, enabling you to run more replicates and more easily include calibration with each test set. With increased calibration frequency, you can have higher confidence in the accuracy of each test run.<div><div><ul><li>Simple and easy&nbsp;to use by adding solvent and injecting</li><li>Shorter preparation time resulting in faster calibration cycle time</li><li>Greater confidence in molecular weight determination of samples</li><li>Reduce variances in analysis providing increased product quality</li></ul><div><div>Recommended Use:&nbsp;For <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/194">advanced polymer chromatography </a>calibration.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_Calibration_Standards_Kits_2_1e32010bc1.jpg" alt="Waters ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards Kits: The ACQUITY Advanced Polymer Chromatography (APC) Calibration Standards are available in polystyrene and polymethylmethacrylate configured into Low, Middle, and High Molecular Weight Calibration Kits. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Advanced_Polymer_Chromatography_APC_Calibration_Standards_Kits_2_1e32010bc1.jpg 245w," sizes="100vw" width="1024" height="565"></div></div></div></div></div></div>

Waters AccQ·Tag Ultra Chemistry kit

Waters AccQ•Tag Ultra kits offer accurate amino acid analysis for diverse matrices, with manual and automated options, optimized columns, stable derivatization, and compatibility with UPLC and robotic pipetting platforms.

<h4>Manual Options</h4>AccQ•Tag Ultra and AccQ•Tag chemistries are the first step in enabling your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and many other sample matrices. Combined with traditional HPLC, UHPLC, or the Waters ACQUITY UPLC, you can now have a system that is customized to separate amino acids from protein hydrolysates, cell culture media, and food/feed samples to get enhanced resolution. The end result is reliability with precise qualitative and quantitative results.AccQ•Tag Ultra and AccQ•Tag chemistry kits include:<ul><li>Pre-column derivatization reagents</li><li>Dedicated reversed-phase columns QC tested for amino acid analysis</li><li>Eluents and standards</li><li>Documentation on methods for the analysis of these important biocompounds</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acc_Q_Tag_Ultra_Chemistry_kit_1_879ccc4140/Waters-AccQTag-Ultra-Chemistry-kit-1.jpg" alt="Waters: AccQ·Tag Ultra Chemistry kit" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acc_Q_Tag_Ultra_Chemistry_kit_1_879ccc4140/thumbnail_Waters-AccQTag-Ultra-Chemistry-kit-1.jpg 234w," sizes="100vw" width="660" height="440"><h4>Automation Options</h4>AccQ•Tag Ultra Derivatization Automation Kit provides simplified tools to enhance high throughput amino acid automation via an easy-to-use, automation friendly format that maximizes workflow and accommodates residual volume. Compatible with Tecan, Hamilton, and Andrew Alliance systems, the AccQ•Tag Ultra Derivatization Automation Kit provides fast, accurate, and precise amino acid analyses, translating into cost and time savings for your lab.<ul><li>Parallel high-throughput sample prep (process up to 96 samples at a time)</li><li>Minimal user intervention</li><li>Larger reagent volume to accommodate automation needs</li><li>Starter kits include reagents, column, eluents, application standards and labware</li><li>Scripts ready for multiple automated platforms<ul><li>Tecan, Hamilton, and Andrew Alliance</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acc_Q_Tag_Ultra_Chemistry_kit_2_36d9723e92/Waters-AccQTag-Ultra-Chemistry-kit-2.jpg" alt="Waters: AccQ•Tag Ultra Derivatization Automation Kit " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acc_Q_Tag_Ultra_Chemistry_kit_2_36d9723e92/thumbnail_Waters-AccQTag-Ultra-Chemistry-kit-2.jpg 240w," sizes="100vw" width="1200" height="780"><h4>Let Andrew+ Pipette for You</h4>One of the largest pain points in sample preparation is repetitive pipetting. Minimizing sources of contamination is also critical. The Andrew+ robotic pipetting platform helps alleviate those pain points and provides confidence and reliability when doing derivatization.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/208">Andrew+ Pipetting Robot</a> provides:<ul><li>Flexibility in the number of samples run using AccQ•Tag Ultra Automation</li><li>Sample Preparation Kit (32, 64, 96 sample)</li><li>Verified protocols available in OneLab</li><li>Compatible with additional connected devices</li><li>Easy-to-use, no in-depth training required</li><li>Self-guided installation, online troubleshooting, and help</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Andrew_Pipetting_Robot_ab82a4584b.jpg" alt="Waters: Andrew+ Pipetting Robot" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Andrew_Pipetting_Robot_ab82a4584b.jpg 245w," sizes="100vw" width="1200" height="726"><h4>Amino Acid Reference Standards</h4>Waters offers reference standards for Amino Acid Analysis (AAA) required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development, and troubleshooting of the AccQ•Tag Ultra and AccQ•Tag methods.Available standards include:<ul><li>Cell Culture Standard Kit</li><li>Food &amp; Feed Standard Kit</li><li>Amino Acid Hydrolysates Standard</li><li>Internal Standard (Nva)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Amino_Acid_Reference_Standards_fd1db2e697.jpg" alt="Waters: Amino Acid Reference Standards" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Amino_Acid_Reference_Standards_fd1db2e697.jpg 234w," sizes="100vw" width="838" height="559"><h4>AccQ•Tag Ultra and AccQ•Tag Derivitization Chemistry</h4>This line of products is a comprehensive, fully tested set of reagents, columns, eluents, and methods. &nbsp;AccQ•Tag is optimized for use with HPLC/UHPLC.AccQ•Tag Ultra is optimized for use with the UPLC Amino Acid Analysis Application Solution.<ul><li>Both methods are based on the AccQ•Tag pre-column derivatization method</li><li>Primary and secondary amino acids are reacted with a single reagent (AccQ•Fluor) in a high-throughput batch process resulting in exceptionally stable derivatives</li><li>AccQ•Tag Ultra provides high-resolution separations achieved using the AccQ•Tag Ultra UPLC column (1.7 µm particles) and mobile phases</li><li>Derivatized amino acids resolved and quantified to sub-pmol levels with single wavelength UV detection</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_kits_9c7f40f8c4.jpg" alt="Waters: AccQ•Tag Ultra and AccQ•Tag Derivitization Chemistry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_kits_9c7f40f8c4.jpg 133w," sizes="100vw" width="352" height="413">

Waters SARS-CoV-2 LC-MS Kit (RUO)

Advance virus research with Waters' RUO LC-MS kit for SARS-CoV-2. Detect and quantify NCAP peptides with high sensitivity and reproducibility using SISCAPA antibody enrichment and automation-ready workflows.

<h4>Detect and quantify SARS-CoV-2 NCAP peptides to advance virus research</h4>The global COVID-19 pandemic created an unprecedented need to quickly deploy emergency tests for SARS-CoV-2 infection. Now, scientists can harness the flexibility and reproducibility of LC-MS to advance critical SARS-CoV-2 research.Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit to directly detect and quantify SARS-CoV-2 signature nucleocapsid peptides (NCAPs) without the need to amplify the target analytes – so you can ensure accuracy, reproducibility, and sensitivity for your most critical research.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg" alt="Waters: SARS-CoV-2 LC-MS Kit (RUO)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/thumbnail_Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg 234w," sizes="100vw" width="660" height="440"><h4>Overview</h4><ul><li>Achieve reproducible results between and within labs &nbsp;</li><li>Ensure high accuracy and analytical sensitivity to enable critical research &nbsp;</li><li>Empower research and development of pioneering SARS-CoV-2 testing with an alternative and complementary technology to common infectious pathogen research &nbsp;</li><li>Enable broader research with an LC-MS method with the potential to be multiplexed with other peptides and biomarkers&nbsp;</li></ul>* This kit is for research use only and has not been approved for use in clinical diagnostic procedures. This RUO kit has not been tested with clinical samples. The SARS-CoV-2 kit for SARS-CoV-2 nucleocapsid peptides is not available in all countries, please contact your Waters representative for more details.Recommended Use: For the detection and quantification of SARS-CoV-2 signature NCAP peptides.<h4>LC-MS enables cutting-edge SARS-CoV-2 research</h4>The Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit intended for the quantitative measurement of three proteolytic peptides derived from the SARS-CoV-2 Nucleocapsid (NCAP) protein. The method has been optimized on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/199">ACQUITY UPLC I-Class PLUS</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/195">Xevo TQ-XS System</a> and is also intended for use with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/208">Andrew+ Pipetting Robot</a>. Optimization on other instrument platforms is possible. &nbsp;Further investigation is needed to collect objective evidence in promising research applications, however, based on the properties of the analytical test method, the SARS-CoV-2 LC-MS Kit might be leveraged for the following research studies: &nbsp;<ul><li>Peptide concentration to quantitate SARS CoV-2 signature peptides at different stages of infection&nbsp;</li><li>Research of infection and long-term effects through peptide quantitation&nbsp;</li></ul>Future pandemic preparedness studies and research of signature peptide-based biomarkers unique to other infections <img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg" alt="Waters: The SARS-CoV-2 LC-MS Kit (RUO) allows for easy sample preparation, sample enrichment, and sample detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Achieve confidence in results</h4>Ensure accurate and consistent results with highly analytically selective antibody magnetic bead enrichment. With the innovative SISCAPA® anti-peptide antibody enrichment sample preparation method from SISCAPA Assay Technologies, Inc. in the SARS-CoV-2 LC-MS Kit (RUO), you can improve the performance of mass spectrometry for measurement of pre-selected protein targets.With the SARS-CoV-2 LC-MS Kit (RUO) and SISCAPA, you can achieve the analytical sensitivity you need to detect low levels of SARS-CoV-2 peptides from complex digested samples with stable isotope standards and capture by anti-peptide antibodies. Increase laboratory efficiency with all the components your busy research laboratory needs to quickly deploy the supplied method - all in one package.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg" alt="Waters: The SARS-CoV-2 method minimizes sample media interference, providing greater flexibility for the analysis of multiple research samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Ensure reproducibility with trusted chemistries and consumables</h4>Ensuring reproducible quantitation from sample-to-sample, run-to-run, and lab-to-lab provides the confidence you need for critical research decisions. Ensuring reproducibility starts with minimizing variability risks, including the risk of unpredictable non-specific binding of peptides to surfaces. The SARS-CoV-2 LC-MS Kit uses QuanRecovery Plates with MaxPeak HPS and a MaxPeak Premier Peptide Column, minimizing non-specific binding of peptides to surfaces while enhancing recovery and signal.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg" alt="Waters: Analytical sensitivity of the ADE, AYN, and NPA peptides in VTM at 2, 3, 5, 10, and 20 amolµL following antibody enrichment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg 234w," sizes="100vw" width="1024" height="682"><h4>Let Andrew+ pipette for you</h4>Save valuable time and focus on your research with reliable and reproducible sample preparation using the Andrew+ Pipetting Robot. The SARS-CoV-2 LC-MS Kit (RUO) comes in an automation-friendly format with liquid handling protocols for the Andrew+ Pipetting Robot available on OneLab.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg" alt="Waters: SARS-CoV-LC-MS Kit (RUO) automation protocol available for Andrew + Pipetting Robot. It provides a flexible automation platform easily adaptable to multiple workflows." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg 234w," sizes="100vw" width="1024" height="682">

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