Tasty beer starts with knowing what’s in your hops. Explore how online SPE makes bitter acid analysis in hops faster and more reliable.<h4>How can you detect bitter acid profiles quickly, reliably, and more sustainably?</h4>For craft brewers and beer lovers alike, a refreshing experience is all about flavor.So how can you achieve the desired bitterness in your beer? The answer is not just about the recipe. Finding the right flavor profile starts with the bitter acids present in the starting hops material.<div><div>Hops (the heart of your favorite beer) are the source of the brew’s distinctive flavor tied to bitterness and aroma.At the molecular level, bitterness is driven by <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0308814622016338">α- and β-bitter acids</a>, like humulones and lupulones, whose concentrations determine the final sensory profile of the beer (Figure 1).Accurately characterizing these compounds in each batch allows brewers to unlock the full potential of every variety of hops.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 1. General chemical structure of hop bitter acids." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_2_6435757c03.jpeg 222w," sizes="100vw" width="700" height="493">While conventional analytical methods rely on long, manual sample preparation steps and hazardous solvents, laboratories now strive for greener and more efficient workflows.And moving from manual to online sample preparation is one of the easiest ways your lab can reduce waste and — most importantly — improve reliability with automation.<h4>Key takeaways</h4><ul><li>Online SPE reduces hands-on work, shortens turnaround time, and supports more sustainable methods by cutting solvent use and eliminating single-use cartridges.</li><li>Automated UHPLC workflows are easier for new users to execute, offering more reproducible quantifications of α- and β-bitter acids in hops.</li><li>Accurate bitter acid profiles help brewers distinguish hop varieties and control flavor with confidence.</li></ul><h4>Comparing offline vs. online solid phase extraction for bitter acid HPLC analysis</h4>If you’ve spent long hours in the lab preparing samples for hops analysis, you’re not alone.Manual sample preparation often takes the most time in hops analysis, an area where online <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/sample-prep.html">solid phase extraction</a> (SPE) can make a real difference. Here’s why.Manual SPE has a lot of manual steps. Extraction. Cartridge loading. Washing. Elution. And sometimes solvent exchange or reduction. All before your actual analysis begins. This labor-intensive workflow can take up to an hour and depends strongly on operator skillsets, making reproducibility a challenge for new hires.<h4>Online SPE analysis provides key benefits like:</h4><ul><li>Hands-off workflow free of cartridges, manifolds, or vacuum steps.</li><li>Column washing and re-equilibration occur in parallel with separation.</li><li>SPE step is complete in just two minutes.</li><li>Minimizes operator-dependent variability.</li><li>Uses less than 8 mL of solvent.</li></ul>Let’s explore what this technique looks like in practice.<h4>Online SPE for analyzing α- and β-bitter acids in hops standard solutions.</h4>Changing your sample preparation from manual to automated isn’t complicated. With the right tools you can fully integrate online SPE with your UHPLC system (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 2. Schematic workflow for the determination of bitter acids in hops. The process involves clean up and instrumental analysis using the Thermo Scientific Vanquish Online SPE UHPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_3_4fb14243b4.jpeg 245w," sizes="100vw" width="700" height="323"><h4>The process for analyzing a hops standard solution is straightforward:</h4><ol><li>First, extract hop samples with ethanol (a greener alternative to toluene or diethyl ether in conventional methods).</li><li>Inject the extract directly into your instrument for analysis. Bitter acids retain<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1970"> on the SPE column</a> while matrix interferences that can comprise quantitation are removed.</li><li>Next, backflush the SPE column to transfer the analytes to the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/423"> analytical column </a>for separation. While this separation is taking place, the SPE column is washed and re-equilibrated in parallel, keeping the total run time to 15 minutes, with the separation of all six major bitter acids in a single analysis (Figure 3).</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 3. UV chromatogram of hop bitter acids in standard solution (1-cohumulone, 2-humulone, 3-adhumulone, 4-colupulone, 5-lupulone, 6-adlupulone)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_4_1e47405e3c.jpeg 245w," sizes="100vw" width="700" height="347"><h4>Online SPE for analyzing α- and β-bitter acids in real hops samples</h4>Analytical labs need methods that are accurate and reliable for brewers to develop distinct and reproducible flavor profiles across batches.To assess the applicability of online SPE UHPLC methods for hops analysis, we analyzed bitter acid profiles from four species — Sultana, Barbe Rouge, Azacca, and Sabro.The concentration of α-acids dominated all samples, while each hops species exhibited distinct profiles in terms of α-acid and β-acid content, making them suitable for different brewing strategies (Figure 4).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg" alt="Thermo Fisher Scientific: How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors: Figure 4. Bitter acid profiles in four different hop species, error bars display SD (n=6). To check method consistency, we measured each hops extract six times. Variation between measurements was low, with relative standard deviation below 3.5 % across all hops species." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_How_Bitter_Acid_Analysis_Helps_Brewers_Better_Control_Beer_Flavors_5_3a179217db.jpeg 245w," sizes="100vw" width="700" height="410"><ul><li>For testing labs, this process translates into a workflow that is easy to run, fast, and delivers reliable results without added complexity.</li><li>For craft brewers, reliability means clear, trustworthy insights into hops bitter acid profiles, for better hop selection and more precise control of bitterness and flavor in your beer.</li></ul><h4>From carefully procured samples to tasty sips</h4>Efficient UHPLC workflows support faster results, less solvent use, and reliable bitterness data for brewers. And online SPE turns those advantages into practical, day-to-day benefits for brewers and labs alike.<ul><li>Less hands-on skills required for lab analysts</li><li>Faster results from labs to brewers</li><li>A more sustainable way to work</li><li>Clear insights into hops flavor</li></ul>To learn more about the method and see the full application data, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cn_004238_hplc_vanquish_online_spe_bitter_acids_hops_cn004238_na_en_587a5d300b.pdf">check out our application note today</a>.<h4>FAQs</h4><div><div><h5>What are the key compounds in hops analyzed by HPLC?</h5>Bitter acids, namely α-acids and β-acids, especially the six major bitter acids (humulones and lupulones) that define hop bitterness and are required for quality control and brewing decisions.</div><div><h5>What column types are recommended for hop acid separations?</h5>Classical C18 Silica columns are ideal for bitter acids separation. UHPLC compatible columns with particle size ≤ 2 µm boost analysis with higher flow rate, thus shorter analysis time and solvent consumption.</div><div><h5>What’s the best workflow for hops analysis?</h5>A typical workflow for hop analysis consists of three main steps: extraction of the target compounds, clean-up of co-extracted matrix components, and instrumental analysis by HPLC or UHPLC. To keep this workflow efficient and reproducible, you should minimize manual handling and add in automation when possible. Integrating online SPE into your LC instrument allows sample clean-up to run automatically, reducing hands-on time, solvent use, and variability while delivering faster, more reliable results.</div></div><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#UHPLC #Automation</blockquote></div></div>

How Bitter Acid Analysis Helps Brewers Better Control Beer Flavors

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

Webinars LabRulezLCMS Week 27/2026

The international standard ISO/IEC 17025 [1] clause 7.7.2 with regards to ensuring the validity of results, requires a laboratory to participate in proficiency testing (PT) and/or to participate in interlaboratory comparisons (ILCs) other than PT. However, no guidance as to what these other ILCs might be, or indeed how they might compare in relation to PT, is provided. This leaflet aims to outline other ILCs available, pointing out some of the limitations as compared to PT.&nbsp;There are three major types of ILCs in which a group of laboratories analyses identical portions from a homogeneous, stable test sample, with each type being characterized by its intended purpose [2,3]:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_2_d9263807fb.jpg 245w," sizes="100vw" width="1507" height="607">A key point is that PT is an ILC that is designed to specifically evaluate the performance of participants, providing them with an infrastructure to monitor and assess the validity of their results. This is not the key purpose of the two other ILCs, and thus depending on their intended purpose they have a number of limitations that laboratories need to be aware of when using them rather than PT. Only proficiency testing should have been conducted in full compliance to the competency standard ISO/IEC 17043 [4].&nbsp;<h4>Evaluation of measurement procedure performance&nbsp;</h4>These ILCs, are designed to assess how a particular measurement procedure performs and whether it is fit for its intended purpose. These ILCs are often referred to as a ‘method performance study’ or a ‘collaborative study’ [2]. Some limitations of using such ILCs for monitoring laboratory performance as an alternative to PT are:&nbsp;<ul><li>All participants use the same measurement procedure i.e. the measurement procedure that is being validated. Unless the measurement procedure is being routinely used by the participating laboratory such an ILC will not assess the routine performance of the laboratory using their own routine measurement procedure. Nor does it enable them to compare their performance with other laboratories using alternative measurement procedures.&nbsp;</li><li>The measurement procedure is likely to be new, so participants may not have established stable procedures and quality control, so performance may not reflect routine use.&nbsp;</li><li>The report from such an ILC would not provide information on the performance of the participant, if the statistical model used makes the assumption that all laboratories perform with equal variability.</li><li>The design of the study may require the participants to assess multiple samples and/or to undertake multiple replicate analysis on the test materials. This may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li><li>The conclusion of the ILC might be that the measurement procedure being assessed is not valid.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg" alt="Eurachem: Interlaboratory comparisons other than proficiency testing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Interlaboratory_comparisons_other_than_proficiency_testing_3_a53ecd2f5d.jpg 157w," sizes="100vw" width="570" height="566"><h4>Characterisation of candidate reference materials&nbsp;</h4>These are ILCs that assign a property value to a candidate reference material, usually with a stated measurement uncertainty. These are often referred to as a ‘material certification study’ [2]. Limitations of using such ILCs as an alternative to PT are:&nbsp;<ul><li>The analysis of the candidate reference material is likely to require additional care in exercising the routine measurement procedure, such as substantially more replication, and/or may require quite different reporting formats (e.g. all valid replicates instead of a mean result). Thus, such an ILC will not necessarily provide an evaluation of the performance of the laboratory based on the routine use of their measurement procedures.&nbsp;</li><li>The report from such an ILC may include the results from the participating laboratories but would not provide information on the performance of each laboratory.&nbsp;</li><li>Participating laboratories may be required to assess multiple samples and/or to undertake multiple replicate analysis on the candidate reference materials. Thus this may not be representative of the routine measurements or tests undertaken by the laboratory.&nbsp;</li></ul><h4>Other ILCs&nbsp;</h4>Some other ILCs, whilst not being conducted in full compliance of the requirements of ISO/IEC 17043, may be designed to evaluate participant performance. These would include small ILCs as defined by EA‑2/21 [5] or split sample ILCs i.e. where a portion of a single sample is sent to two or three laboratories. Whilst these ILCs are specifically designed to evaluate participant performance, they may not provide a comprehensive comparison that a PT might provide. In such cases the laboratory needs to evaluate carefully the competence of the organization managing the ILC and the suitability of the service externally provided (ISO/IEC 17025 clause 6.6).

Interlaboratory comparisons other than proficiency testing

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Characterization of polymers (Watrex)

Watrex Prague offers laboratory characterization of polymers using GPC.

<h4>WI08112 – Determination of Molecular Weight Distribution of Polymeric Materials</h4><ul><li>Determination by GPC method in water and THF, or optionally in other solvents upon request.</li><li>Each set of measurements requires calibration (WI08113).</li><li>Price: 3,300 CZK excl. VAT</li></ul><h4>WI08113 – Calibration for Molecular Weight Distribution of Polymeric Materials</h4><ul><li>Calibration using dextran, polymaltotriose, or polystyrene standards for GPC molecular weight distribution determination.</li><li>Price: 4,500 CZK excl. VAT</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_GPC_separace_polymeru_na_kolone_Watrex_Delta_Gel_47ce92deaa.jpg" alt="Watrex: GPC separation of polymers on Watrex DeltaGel column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_GPC_separace_polymeru_na_kolone_Watrex_Delta_Gel_47ce92deaa.jpg 213w," sizes="100vw" width="1385" height="1018"><blockquote><h3>Services – Laboratory</h3>The Watrex Analytical Laboratory will be happy to perform any of our standard analyses, custom analyses, or assist you with method development.We specialize primarily in liquid chromatography techniques and are ready to meet a wide range of requirements—from method development and optimization, through validation and verification of external methods, to training and troubleshooting.Our laboratory also manufactures and packs its own chromatographic columns and guard columns for basic HPLC modes (reversed phase, normal phase, ion chromatography, and ion exclusion chromatography).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Analyticka_laborator_fc8862fba5.jpg" alt="Watrex: Analytical Laboratory" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Analyticka_laborator_fc8862fba5.jpg 245w," sizes="100vw" width="1569" height="637"></blockquote>

Analytical Services for Building Materials (Watrex Laboratory)

Comprehensive analysis of building materials by ion chromatography and gravimetry – salts, moisture, lime, gypsum, density, and absorption testing by Watrex Laboratory.

<h5>WI08101 – Determination of Water-Soluble Salts in Building Materials</h5><ul><li>Determination of chlorides, nitrates, and sulfates in aqueous extract using ion chromatography. Requires approx. 1 g of a homogeneous sample.</li><li>Price: 600 CZK excl. VAT</li></ul><h5>WI08102 – Determination of Moisture in Building Materials</h5><ul><li>Gravimetric method after drying the sample to constant weight. Moisture content is expressed in mass %. Requires at least 10 g of sample in sealed packaging.</li><li>Price: 300 CZK excl. VAT</li></ul><h5>WI08103 – Determination of Lime Content in Lime Mortars</h5><ul><li>After acid digestion, Ca and Mg content is determined to calculate CaCO₃ and MgCO₃. Sand content determined gravimetrically. Not suitable for cement mortars. Requires approx. 5 g of sample.</li><li>Price: 1,275 CZK excl. VAT</li></ul><h5>WI08104 – Semi-Quantitative Determination of Gypsum in Building Materials</h5><ul><li>Based on solubility differences of gypsum and alkaline sulfates. Requires approx. 11 g of sample.</li><li>Price: 1,100 CZK excl. VAT</li></ul><h5>WI08105 – Determination of Bulk Density of Building Materials</h5><ul><li>Performed using quartz sand displacement. Requires 30–50 cm³ of sample in pieces 2–4 cm.</li><li>Price: 440 CZK excl. VAT</li></ul><h5>WI08109 – Determination of Hygroscopic Moisture in Building Materials</h5><ul><li>Gravimetric method after equilibrium at 100% RH and drying at 105 °C for 2 hours. Requires approx. 10 g of sample.</li><li>Price: 440 CZK excl. VAT</li></ul><h5>WI08114 – Determination of Water Absorption of Building Materials</h5><ul><li>Gravimetric method from weight difference between dried and water-saturated sample. Bulk density determined simultaneously. Requires 30–50 cm³ of sample in pieces 2–4 cm.</li><li>Price: 4,495 CZK excl. VAT</li></ul><blockquote>10% discount when submitting 3 or more samples at once.</blockquote><blockquote><h3>Services – Laboratory</h3>The Watrex Analytical Laboratory will be happy to perform any of our standard analyses, custom analyses, or assist you with method development.We specialize primarily in liquid chromatography techniques and are ready to meet a wide range of requirements—from method development and optimization, through validation and verification of external methods, to training and troubleshooting.Our laboratory also manufactures and packs its own chromatographic columns and guard columns for basic HPLC modes (reversed phase, normal phase, ion chromatography, and ion exclusion chromatography).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Analyticka_laborator_fc8862fba5.jpg" alt="Watrex: Analytical Laboratory" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Analyticka_laborator_fc8862fba5.jpg 245w," sizes="100vw" width="1569" height="637"></blockquote>

Advanced PFAS Analytical Services (ALS)

Comprehensive PFAS analysis in water, soil, sludge, and AFFF sites using LC-MS/MS. Detect 60+ PFAS compounds, meet EU limits, and ensure compliance with evolving 2025 regulations.

<h4>Advanced PFAS Analytical Services</h4><h5>Ensuring Compliance, Safety and Environmental Responsibility</h5>Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals widely used in industrial applications and consumer products. Their persistence in the environment and potential health risks have led to increasing regulatory scrutiny, particularly in drinking water and firefighting foam residues.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Advanced_PFAS_Analytical_Services_c8d8b28b7c.jpg" alt="ALS: Advanced PFAS Analytical Services" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Advanced_PFAS_Analytical_Services_c8d8b28b7c.jpg 245w," sizes="100vw" width="1604" height="502"><h4>Our Expertise</h4>We offer state-of-the-art analytical services for the detection and quantification of PFAS in:<ul><li>Drinking/ground/surface/waste water</li><li>Soil, sediment, sludge and biosolids</li><li>AFFF-contaminated sites and products</li></ul><h5>Using LC-MS/MS, our laboratories provide:</h5><ul><li>Ultra-trace level detection (ng range)</li><li>Analysis of over <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_01_europe_the_scope_of_pfas_analyses_in_waters_and_current_state_of_legislation_6ebd4f1b6f/enviromail_01_europe_the-scope-of-pfas-analyses-in-waters-and-current-state-of-legislation.pdf">60 individual PFAS compounds</a></li><li>Non-targeted PFAS analysis like TOPA</li></ul><blockquote><h5>Contact Us</h5>Let us help you navigate the complex landscape of PFAS regulation and ensure your operations meet the highest standards of safety and compliance.<ul><li>+420 226 226 228</li><li><a href="mailto:customer.support@alsglobal.com">customer.support@alsglobal.com</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.eu/">alsglobal.eu</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_PFAS_421ef75bea.jpg" alt="ALS: PFAS Analytical Services" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_PFAS_421ef75bea.jpg 156w," sizes="100vw" width="919" height="919"></blockquote><h4>Regulatory Context in Europe (2025)</h4>European legislation is rapidly evolving to address PFAS contamination:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/legal-content/EN/LSU/?uri=oj:JOL_2020_435_R_0001">The EU Drinking Water Directive (2020/2184) </a>mandates monitoring of PFAS, with a parametric value of 0.10 μg/L for the sum of 20 PFAS compounds</li><li><a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=OJ:L:2006:396:TOC">Reach Regulation 1907/2006</a> and the key <a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/eli/reg/2021/1297/oj/eng">Amendment Regulation 2021/1297</a>, targeting the C9-C14 perfluorocarboxylic acids and related substances</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/04/10/soil-monitoring-law-council-reaches-deal-with-parliament/">The Soil Monitoring law</a> (provisional agreement april 2025) includes PFAS as part of the soil Health assessment</li><li><a target="_blank" rel="noopener noreferrer" href="https://environment.ec.europa.eu/topics/water/water-framework-directive_en">The Water Frame Directive </a>with EQS on surface and groundwater and considerations for sediments and biota</li></ul><h3>List of PFAS analytes and report limits for determining PFAS substances in water</h3>For the sum of PFAS, a limit of 0.1 μg/l has been set, which applies to 20 substances marked with an asterisk (*) in the text below.<h5>Group: Perfluoroalkyl carboxylic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluorobutanoic acid / PFBA* / 0.01 / 0.002</li><li>Perfluoro-3-methoxypropanoic acid / PFMPA / 0.025 / 0.001</li><li>Perfluoropentanoic acid / PFPeA* / 0.01 / 0.0003</li><li>Perfluoro-4-methoxybutanoic acid / PFMBA / 0.025 / 0.001</li><li>Perfluorohexanoic acid / PFHxA* / 0.01 / 0.0003</li><li>Tetrafluoro-2-(heptafluoropropoxy) propanoic acid / HFPO-DA / 0.02 / 0.001</li><li>Perfluoroheptanoic acid / PFHpA* / 0.01 / 0.0003</li><li>4,8-dioxa-3H-perfluorononanoic acid / DONA / 0.01 / 0.002</li><li>7H-perfluoroheptanoic acid / HPFHpA / 0.01 / 0.001</li><li>Perfluorooctanoic acid / PFOA* / 0.005 / 0.0003</li><li>Perfluoro-3,7-dimethyloctanoic acid / P37DMOA / 0.01 / 0.001</li><li>Perfluorononanoic acid / PFNA* / 0.01 / 0.0003</li><li>Perfluorodecanoic acid / PFDA* / 0.01 / 0.0003</li><li>2H,2H,3H,3H-Perfluorooctanoic acid / H4PFUnDA / 0.02 / 0.0003</li><li>Perfluorooctanoic acid / PFUnDA* / 0.01 / 0.0003</li><li>Perfluorododecanoic acid / PFDoDA* / 0.01 / 0.0003</li><li>Perfluorotridecanoic acid / PFTrDA* / 0.01 / 0.0003</li><li>Perfluorotetradecanoic acid / PFTeDA / 0.025 / 0.0003</li><li>Perfluorohexadecanoic acid / PFHxDA / 0.05 / n.a.</li><li>Perfluorooctadecanoic acid / PFOcDA / 0.05 / n.a.</li></ul><h5>Group: Perfluoroalkylsulfonic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluoropropanesulfonic acid / PFPrS / 0.02 / 0.001</li><li>Perfluoro(2-ethoxyethane)sulfonic acid / PFEESA / 0.1 / 0.001</li><li>Perfluorobutanesulfonic acid / PFBS* / 0.01 / 0.0003</li><li>Perfluoropentanesulfonic acid / PFPeS* / 0.01 / 0.0003</li><li>Perfluorohexanesulfonic acid / PFHxS* / 0.01 / 0.0003</li><li>Perfluoroheptanesulfonic acid / PFHpS* / 0.01 / 0.0003</li><li>Perfluorooctane sulfonic acid / PFOS* / 0.005 / 0.0003</li><li>n-Decafluoro-4-(pentafluoroethyl) cyclohexane sulfonic acid / PFECHS / 0.01 / 0.0003</li><li>Perfluorononane sulfonic acid / PFNS* / 0.01 / 0.0003</li><li>Perfluorodecane sulfonic acid / PFDS* / 0.01 / 0.0003</li><li>Perfluorooctadecane sulfonic acid / PFUnDS* / 0.01 / 0.001</li><li>Perfluorododecane sulfonic acid / PFDoDS* / 0.01 / 0.0003</li><li>Perfluorotridecane sulfonic acid / PFTrDS* / 0.02 / 0.001</li></ul><h5>Group: Fluorotelomeric sulfonates</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>4:2 fluorotelomeric sulfonate / 4:2 FTS / 0.01 / 0.0003</li><li>6:2 fluorotelomeric sulfonate / 6:2 FTS / 0.01 / 0.0003</li><li>8:2 fluorotelomeric sulfonate / 8:2 FTS / 0.01 / 0.0003</li><li>10:2 fluorotelomeric sulfonate / 10:2 FTS / 0.01 / n.a.</li></ul><h5>Group: Perfluorinated sulfonamides</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>N-methyl-perfluorooctanesulfonamide / MeFOSA / 0.05 / 0.002</li><li>N-ethyl perfluorooctane sulfonamide / EtFOSA / 0.05 / 0.002</li><li>N-methyl perfluorooctane sulfonamidoethanol / MeFOSE / 0.025 / 0.002</li><li>N-ethyl perfluorooctane sulfonamidoethanol / EtFOSE / 0.025 / 0.002</li></ul><h5>Group: Perfluorooctane sulfonamidoacetic acid derivatives</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluorooctane sulfonamidoacetic acid / FOSAA / 0.01 / 0.001</li><li>N-Methyl perfluorooctane sulfonamidoacetic acid / MeFOSAA / 0.01 / 0.001</li><li>N-Ethyl perfluorooctane sulfonamidoacetic acid / EtFOSAA / 0.01 / 0.001</li></ul><h5>Group: Fluorotelomeric carboxylic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>3-Perfluoropropyl propanoic acid (3:3) / 3:3 FTCA / 0.1 / 0.0003</li><li>n-2-Perfluorohexyl ethanoic acid (6:2) / 6:2 FTCA / 0.1 / 0.001</li><li>3-Perfluoropentyl propanoic acid (5:3) / 5:3 FTCA / 0.02 / 0.001</li><li>2H-Perfluoro-2-octenoic acid (6:2) / 6:2 FTUCA / 0.02 / 0.001</li><li>3-Perfluoroheptyl propanoic acid (7:3) / 7:3 FTCA / 0.02 / 0.0003</li><li>n-2-Perfluorooctyl ethanoic acid (8:2) / 8:2 FTCA / 0.1 / 0.001</li><li>2H-Perfluoro-2-decanoic acid (8:2) / 8:2 FTUCA / 0.02 / 0.001</li></ul><h5>Group: Chlorinated perfluoroalkyl sulfonic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid / 9Cl-PF3ONS / 0.01 / 0.001</li><li>11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid / 11Cl-PF3OUdS / 0.01 / 0.002</li></ul>*Analytes included in the sum of 20 PFAS in drinking water according to DIRECTIVE (EU) 2020/2184 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2020 on the quality of water intended for human consumption.<h4>Applications</h4><ul><li>Site investigations and remediation planning</li><li>Compliance monitoring for water utilities</li><li>Environmental due diligence for industrial sites</li><li>AFFF legacy contamination assessments</li><li>Products compliance</li></ul><h4>Why Choose Us?</h4><ul><li>Accredited laboratories with experience in regulatory, diagnostic and scientific PFAS analyses</li><li>Rapid turnaround times and customizable reporting formats</li><li>Expert support for laboratories, environmental consultants, utilities, and industrial clients</li><li>Webinars by specialists in the field</li></ul><blockquote><h5>Read more about PFAS analysis:</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_02_europe_sampling_recommendations_for_pfas_to_maximize_data_quality_26c1da00bb/enviromail_02_europe_sampling-recommendations-for-pfas-to-maximize-data-quality.pdf">EnviroMail™ 02/Europe: Sampling Recommendations for PFAS to Maximize Data Quality</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_10_europe_pfas_forever_chemicals_in_soil_a5b49ad7dd/enviromail_10_europe_pfas_forever-chemicals-in-soil.pdf">EnviroMail™ 10/Europe: PFAS: “Forever chemicals” in soil</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_13_europe_passive_sampling_for_the_monitoring_of_pfas_in_water_feb2024_227a69e2dc.pdf">EnviroMail™ 13/Europe: Passive sampling for the monitoring of PFAS in water</a></li></ul></blockquote>

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