At MSACL 2025, a Thermo Fisher Scientific–sponsored workshop led by Qin Fu addressed one of the most persistent challenges in proteomics: how to efficiently and reproducibly translate biomarker discoveries into clinically meaningful assays. Drawing on experience spanning academia and industry—including prior work in Jennifer Van Eyk’s lab—Fu shared practical insights into building a scalable, end-to-end biomarker discovery-to-validation pipeline.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Accelerating_Biomarker_Discovery_and_Validation_From_Discovery_to_Clinical_Utility_9558364cf2.jpg" alt="Thermo Fisher Scientific: Accelerating Biomarker Discovery and Validation: From Discovery to Clinical Utility" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Accelerating_Biomarker_Discovery_and_Validation_From_Discovery_to_Clinical_Utility_9558364cf2.jpg 245w," sizes="100vw" width="1024" height="512">The session highlighted how emerging mass spectrometry technologies, automation, and informatics are reshaping biomarker research—reducing complexity, improving reproducibility, and accelerating the path to clinical utility.<h3>The three tiers of biomarker assays</h3>The workshop opened with a review of the widely accepted three-tier framework that guides biomarker development from early discovery through clinical validation:<h4>Tier 3 – discovery</h4>Exploratory, untargeted workflows such as data-dependent acquisition (DDA) and data-independent acquisition (DIA). These approaches prioritize breadth, enabling the identification of large-magnitude biological changes across hundreds to thousands of analytes, typically with low-to-moderate precision and without internal standards.<h4>Tier 2 – verification</h4>Research-grade targeted assays, including parallel reaction monitoring (PRM) and multiple reaction monitoring (MRM). These methods focus on tens to hundreds of analytes, delivering improved precision and repeatability to confirm trends observed during discovery.<h4>Tier 1 – validation</h4>Clinically validated assays designed to meet rigorous quality and regulatory standards. These workflows incorporate internal standards and strict quality controls to ensure quantitative reproducibility suitable for clinical and translational use.Each tier represents a critical step toward ensuring that biomarker candidates can ultimately deliver reliable patient benefit.<h3>The central challenge: from sample to signal</h3>Reliable biomarker development depends on consistency across the entire analytical workflow—from biological sample preparation to mass spectrometry data acquisition and downstream analysis. Every step introduces potential sources of variability, and minimizing this analytical noise while preserving biological signal is essential.Automation emerged as a central theme of the workshop. Beyond improving throughput, automation fundamentally enhances reproducibility. Robotic sample preparation reduces variability associated with digestion, pipetting, and handling—enabling consistent performance across hundreds or thousands of samples, a scale that is difficult to achieve manually.<h3>Technological innovation powering a paradigm shift</h3>Recent advances in mass spectrometry are enabling researchers to bridge the gap between discovery and validation more efficiently than ever before. The workshop highlighted several Thermo Fisher Scientific platforms driving this shift:<ul><li>Thermo Scientific Orbitrap Astral mass spectrometer Optimized for discovery workflows, the Astral system delivers rapid acquisition and deep proteome coverage while maintaining high precision across large cohorts.</li><li>Thermo Scientific Stellar platform Designed for high-throughput targeted quantification, the Stellar platform supports scalable PRM workflows. Features such as PRM Conductor enable adaptive retention time tracking, which is essential for robust performance in large studies.</li><li>Thermo Scientific OptaSpray Ion Source (under evaluation) A hands-free ionization source designed to deliver consistent spray performance with simplified maintenance, supporting reproducibility across thousands of injections.</li></ul>Together, these technologies create a smoother continuum across assay tiers, shortening timelines and reducing barriers to clinical translation.<h3>Case study: Inflammatory bowel disease (IBD) cohort</h3>A key highlight of the presentation was a large-scale biomarker study in inflammatory bowel disease (IBD). The team conducted a 500-sample discovery analysis, followed by validation in a 1,000-sample cohort. Discovery was performed using the Orbitrap Astral system, while targeted validation employed the Stellar platform.This integrated approach successfully confirmed established biomarkers such as C-reactive protein (CRP) while also identifying novel protein candidates with strong translational potential. Importantly, these markers demonstrated consistency across discovery and validation data sets—underscoring the power of advanced instrumentation combined with robust workflows and informatics.<h3>From high quantity to high quality</h3>Historically, biomarker discovery has involved a tradeoff between throughput and data quality. The workshop demonstrated that this compromise is no longer necessary. By integrating automated sample preparation, optimized informatics, and next-generation mass spectrometry, researchers can now generate high-quality, high-throughput data that is both scalable and reproducible.This shift enables faster discovery while increasing confidence that candidate biomarkers are ready for downstream validation and clinical follow-up.<h3>The future of biomarker translation</h3>The workshop concluded on an optimistic note. Innovations in proteomics are fundamentally transforming biomarker development—shortening timelines, reducing costs, and accelerating the transition from discovery to clinical application.By integrating platforms such as Orbitrap Astral, Stellar, and OptaSpray, Thermo Fisher Scientific is helping advance a new era of translational biomarker science—where precision, scalability, and reliability work together to deliver meaningful clinical impact.Watch the full workshop recording to <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=ziyffQzZTwk">explore the data, workflows, and results behind this end-to-end approach to biomarker discovery and validation.</a><h3>Intended use disclaimer</h3>General Laboratory Equipment – Not For Diagnostic Procedures. Thermo Scientific general laboratory equipment, consumables, and software are intended for research, academic, and industrial use only. These products are not designed, validated, or approved for in vitro diagnostic or clinical use. Use of these products for diagnostic purposes may result in inaccurate results and potential health and safety risks.<h3>Resources</h3>Workshop &amp; educational content<ul><li>MSACL 2025 workshop recording: <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=ziyffQzZTwk">Accelerating Biomarker Discovery and Validation</a></li><li>Other MSACL workshop recordings coming soon</li></ul>Thermo Fisher Scientific Product Pages<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2207">Thermo Scientific TSQ Certis Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/836">Thermo Scientific Orbitrap Astral Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar Mass Spectrometer</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/B51004132">Thermo Scientific OptaSpray Ion Source</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/mass-spectrometer-price-affordability-programs.html">Mass spectrometer affordability programs</a> (Refurbished MS, trade-in, financing/ leasing)</li></ul>Peer-Reviewed Literature<ul><li>Baker ES et al. <a target="_blank" rel="noopener noreferrer" href="https://europepmc.org/article/MED/22943415">Mass spectrometry for translational proteomics: progress and clinical implications.</a> Genome Medicine (2012).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://academic.oup.com/clinchem/article/62/1/198/5611803">An Empirical Approach to Signature Peptide Choice for Selected Reaction Monitoring.</a> Clinical Chemistry (2016).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/mas.21750">Automated proteomic sample preparation: The key component for high throughput and quantitative mass spectrometry analysis.</a> Mass Spectrometry Reviews (2023).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.jproteome.7b00623">Highly Reproducible Automated Proteomics Sample Preparation Workflow for Quantitative Mass Spectrometry.</a> Journal of Proteome Research (2018).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202409446">A Proteomics Pipeline for Generating Clinical Grade Biomarker Candidates from DIA-MS Discovery.</a> Angewandte Chemie International Edition (2024).</li><li>Fu Q et al. <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/anie.202507610">Development and Clinical Evaluation of a Multiplexed Health Surveillance Panel Using Ultra High-Throughput PRM-MS in an Inflammatory Bowel Disease Cohort.</a> Angewandte Chemie International Edition (2025).</li></ul><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#Omics #BiomarkerDiscovery #MassSpectrometry #ClinicalResearch</blockquote>

Accelerating Biomarker Discovery and Validation: From Discovery to Clinical Utility

Interlaboratory comparisons (ILCs) have been used for more than a century and many important concepts are linked to them. This leaflet addresses the basic terminology of ILCs, why some colloquial terms can be misleading or misunderstood, and the importance of harmonisation.&nbsp;An ‘ILC’ is the organisation, performance and evaluation of measurements or tests on the same or similar items by two or more laboratories in accordance with predetermined conditions [1]. ILCs have a variety of overall – and detailed – objectives, the three most common [2] being:<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Let_s_call_a_PT_scheme_a_PT_scheme_3_9993b9fb0f.jpg" alt="Eurachem: Let’s call a PT scheme a PT scheme" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Let_s_call_a_PT_scheme_a_PT_scheme_3_9993b9fb0f.jpg 245w," sizes="100vw" width="1516" height="472"><h4>Some comparisons have special names&nbsp;</h4>The evaluation of participant (laboratory, organisation or individual) performance is typically referred to as ‘proficiency testing’ (PT) or ‘external quality assessment’ [1]. When the main objective is evaluating the performance of, for example, a candidate standard method, the ILC is often called a ‘method performance study’ or a ‘collaborative study’ [2, 3]. The former is quite clear whereas wording such as “collaborative” and “cooperative” just stresses joint effort. A project aimed at producing a new certified reference material may involve several laboratories conducting analyses on agreed properties [4]. Not surprisingly, this work is sometimes labelled as a ‘material certification study’. Special ILCs used to demonstrate measurement capabilities of National Metrology Institutes are called ‘key comparisons’, ‘supplementary comparisons’, and ‘pilot studies’ [5].&nbsp;<h4>Some terms can be confusing&nbsp;</h4>Other terms for ILCs, including ‘ring test’, ‘round robin’ and ‘circle analysis’, are common in the literature. They have, however, been used for different objectives and different designs and may be ambiguous or understood differently by laboratory staff. The use of these terms may, for example, imply a specific way of distributing the ILC sample. ISO/IEC 17043 uses ‘sequential scheme’, for a PT where a unique item is transferred between the participants and ‘simultaneous scheme’, in which similar items from a batch are distributed simultaneously to the participants [1].&nbsp;Terms like ‘measurement audit’, ‘interlaboratory testing scheme’, ‘measurement comparison scheme’, ‘rapid performance evaluation scheme’ and ‘intercalibration’ may not correctly reflect the category of laboratory involved, or the type of work participants do during the ILC.&nbsp;When in 1994 the IUPAC defined and described three major types of ‘interlaboratory studies’, the word ‘study’ was preferred to synonyms such as ‘trial’, ‘exercise’, ‘test’, ‘evaluation’ or ‘check’ [2].<h4>Let’s try to harmonise&nbsp;</h4>Many laboratory staff are unaware of official terms related to ILCs but recognise the acronym PT and words like ‘ring test’. Ideally, we would use a common scientific language, but harmonisation is time consuming. Terms appear and disappear and definitions change. The same term can be defined differently, even in different international standards. Lack of standards and guidelines in local languages can cause confusion and contribute to spreading of less appropriate terms.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Eurachem_Let_s_call_a_PT_scheme_a_PT_scheme_2_820043ce8b.jpg" alt="Eurachem: Let’s call a PT scheme a PT scheme" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Eurachem_Let_s_call_a_PT_scheme_a_PT_scheme_2_820043ce8b.jpg 234w," sizes="100vw" width="1006" height="671">Much confusion can be avoided by clear reference to relevant standards and sectoral guides. ISO/ IEC 17043 [1] and ISO 13528 [6] contain many important concepts related to PT schemes and other ILCs. Some of these are also incorporated into ISO/IEC 17025 [7]. Additional useful, and free, tools are the Eurachem PT Guide [8] and the ISO online browsing platform [9].&nbsp;In writing guidance and instructions, remember that the term ‘interlaboratory comparison’ has a broad meaning and can be used for all cases described above. Sometimes this is clear enough for the reader. In other cases, use acknowledged terms that reflect the detailed objective of the ILC. Let’s call a PT scheme a PT scheme!

Let’s call a PT scheme a PT scheme!

The countdown is on for one of the world’s leading conferences dedicated to food analysis. The 12th International Symposium on Recent Advances in Food Analysis (RAFA 2026), taking place in Prague from 3–6 November 2026, is approaching two important milestones: the Early Bird registration deadline and the deadline for oral abstract submissions, both ending on 30 June 2026. Researchers, analysts, industry professionals, regulators, and students are encouraged to secure their participation at a reduced registration rate and take advantage of the opportunity to present their latest scientific results to the international food analysis community.RAFA 2026 will once again bring together leading experts from academia, industry, and regulatory organizations to discuss the latest developments in food safety, authenticity, contaminants, non-target screening, PFAS, microplastics, food contact materials, artificial intelligence, and advanced analytical technologies. The preliminary program already features an outstanding lineup of internationally recognized speakers addressing some of the most pressing challenges in modern food analysis and food safety.<h3>Registration</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/">Register to attend RAFA 2026!</a>Early-bird registration until 30 June, 2026</blockquote>After successful submission of your registration, you will receive by e-mail (i) an automatic confirmation of receipt indicating the total amount of payment due (either for bank transfer or for on-line payment by card), (ii) login to Member section where you can administrate your profile, submit your abstracts, and from September 2026 register for satellite events, workshops and seminars. If it will not be delivered within a few minutes after submission of your registration please contact us at RAFA2026@vscht.cz.As soon as payment of registration fee is processed, you will also receive by e-mail (iii) confirmation of payment.<h4>Registration Fees</h4><ul><li>Smart rate (Until 30 June, 2026): € 655</li><li>Regular registration (Until 30 September, 2026): € 855</li><li>Late registration (From 1 October, 2026): € 955</li><li>Student rate*&nbsp;<ul><li>Until 30 June, 2026: € 465</li><li>From 1 July, 2026: € 605</li></ul></li></ul>* The proof of student status (e.g. copy of a student identify card) is requested as an attachment of the registration form.<h5>The regular registration fee will include:</h5><ul><li>Attendance at all scientific sessions, workshops, seminars, vendor seminars and other satellite events</li><li>Access to the poster and exhibition area</li><li>Conference kit (name badge, conference bag, conference documents, certificate of participation, certificate of presentation on request)</li><li>Final programme</li><li>E-Book of Abstracts</li><li>Coffee breaks</li><li>Buffet luncheons</li><li>Welcome drink</li></ul>In the registration form you can register free of charge for the welcome drink and you can also order ticket(s) for the conference dinner (€ 85).Please note that your registration is firm when your payment has been received.<h3>Abstract submission</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://rafa.easyevent.eu/tickets/">Submit your ABSTRACT HERE!</a>In order to submit an abstract, you must first register to attend RAFA 2026. After registration you will receive an access to the Member section where you can submit your abstract(s).</blockquote>&nbsp;After successful submission of your abstract you will receive an automatic confirmation of receipt of submitted abstract via e-mail. If it will not be delivered within a few minutes after submission of your abstract please check at first your spam box-mail and then contact us at RAFA2026@vscht.cz.Please ensure that your abstract does not contain spelling, grammatical or scientific errors. Even though modifications of your submitted abstract will be allowed in the Abstract section of the Member section until the deadline for the abstract submission, please be confident on the lack of errors in your final version prior to starting the submission process.The abstract will be reproduced exactly as submitted. No proofreading will be done, although some corrections may be requested by the reviewers.If you need to withdraw your abstract, a written statement reflecting the reasons for this decision must be sent to RAFA2026@vscht.cz no later than September 30, 2026.<h4>Important notice:</h4><blockquote>1 registered participant can submit no more than 2 abstracts.</blockquote>To apply for a young scientist’s oral presentation, as an indication please respect the rule that you should be born in 1991 or later.The text of the abstract should be limited to 3000 characters including spaces. Tables and pictures are not allowed.Abstracts of oral presentations and posters will be included into the "e-Book of Abstracts" only after receipt of the conference registration fee. Any payment of the registration fee has to be received until September 30, 2026, otherwise contribution(s) will not be included into the e-Book of Abstracts and the Conference programme.<h3>Program</h3><h4>Invited Speakers</h4><h5>Food allergen risk assessment: Past, present, future</h5><ul><li>Ben Remington from Remington Consulting Group B.V., Utrecht, the Netherlands &amp; University of Nebraska, USA</li></ul>Food allergy and allergen management are global public health issues, and consumer protection depends on transparent and trustworthy labelling. While 30 years of scientific advances show that low level exposures, or reference doses, are unlikely to trigger reactions in most allergic individuals, harmonized and globally aligned regulations are still needed to promote consistent allergen risk assessment and labelling decisions. This presentation will discuss the landscape of allergen risk assessment, global policy developments, current research and future needs in the area.<h5>Unmasking hidden adulteration in honey: Strategies for detecting and characterizing honeys produced from sugar-fed colonies</h5><ul><li>Oscar Nuñez Burcio from University of Barcelona, Barcelona, Spain</li></ul>From hive to jar, not all honey tells the same story. This presentation addresses hidden adulteration associated with sugar-fed bee colonies, highlighting implications for authenticity, production practices, and the need for a more precise characterization of honey to ensure consumer trust.<h5>Analytical and toxicological methods for safety of food contact materials</h5><ul><li>Erich Leitner from Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, Graz, Austria</li></ul>Food packaging plays a critical role in protecting and preserving food throughout the supply chain. All packaging materials intended to come into contact with food must comply with strict EU safety rules. Due to increased complexity of packaging solutions, lowering legal limits and increasing numbers of target compounds, we also have to adapt the strategies used to verify and guarantee the safety of these materials.<h5>Data-driven strategies to reduce complexity in non-target HRMS analysis of food samples</h5><ul><li>Anneli Kruve from Stockholm University, Stockholm, Sweden</li></ul>LC/HRMS based non-target screening can detect hundreds to thousands of chemical features in complex food and environmental samples. Investigating all of these in detail is practically impossible, so which ones to focus on? Novel computational tools developed in computational mass spectrometry allow predicting the toxicity and concentration of the detected chemical features even if these have not yet been fully structurally elucidated. Combining these predictions enables us to focus on the chemical features possessing high risk.<h5>Role of AI in the identification of emerging food systems risks</h5><ul><li>Ákos Bernard Jóźwiak from Syreon Research Institute, Budapest, Hungary</li></ul>AI-assisted evidence synthesis – spanning curated scientific evidence, exposome data, and network analysis – is the backbone of next-generation emerging chemical food safety risk identification, enabling earlier signals and more resilient surveillance systems.<h5>Challenges of microplastics analysis in the context of food safety</h5><ul><li>Dorte Herzke from Norwegian Institute of Public Health, Oslo, Norway</li></ul>Food and food packaging are major sources of micro- and nanoplastic exposure. Plastics used in processing, storage, and packaging can release particles through heat, abrasion, and degradation. Small particles below 2 µm may cross gut barriers and carry chemicals, highlighting the need for robust and sensitive measurement methods.<h5>Never-ending analytical challenges in PFAS analysis of food</h5><ul><li>Darina Dvořáková from University of Chemistry and Technology Prague, Prague, Czechia</li></ul>Exploring and monitoring PFAS in food requires advanced strategies. Combining targeted, suspect, and non-target HRMS analysis, determination of total fluorine and sum parameters, and novel sensors provides new insights into PFAS occurrence and supports food safety monitoring.<h5>An overview of the latest scientific knowledge on process contaminants in food for appropriate risk management measures</h5><ul><li>Thierry Delatour from Nestlé Research, Lausanne, Switzerland.</li></ul>Recent scientific findings related to acrylamide and furans formation, occurrence and analytical methods in food provide important insights for risk management.

Deadline for RAFA 2026 Early Registration and Oral Abstract Submission is approaching

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RAFA provides an overview of contemporary trends in ANALYTICAL & BIOANALYTICAL STRATEGIES IN FOOD QUALITY AND SAFETY CONTROL and discusses CHALLENGES / NOVEL APPROACHES IN FOOD AND NATURAL PRODUCTS ANALYSIS.

Recent Advances in Food Analysis (RAFA)

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Eurachem is a European network promoting international traceability of chemical measurements and good analytical practice. It provides expert guidance, supports method validation, uncertainty evaluation, proficiency testing, and facilitates discussion on technical and policy issues in analytical quality.

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KNAUER LH 8.1 - Modular analytical XYZ Autosampler

KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed.

KNAUER Liquid Handler offers finally an alternative in the world of robotic autosamplers. In-house design and development for a new level of simplicity and speed, namely by:<h4>Performance</h4><ul><li>Highest repeatability of peak area in full loop or sandwich injection mode</li><li>Zero sample loss and high precision via Sandwich injection mode&nbsp;</li><li>Lowest carry over with active Fast wash station that cleans the injection syringe and needle on the in- and outside by delivering an adjustable cleaning solvent flow</li><li>User-friendly control via pre programmed injection parameters in Chromatography Software packages</li></ul><h4>Versatility</h4><ul><li>Modular design and wide selection of accessories</li><li>Flexible injection volumes by a variety of exchangeable injection syringes&nbsp;</li><li>Customizable lengths of the rail from standard &nbsp;557 or 887 mm up to 1200mm to increase the amount of modules</li></ul><h4>High throughput</h4><ul><li>Up to 390 x 1.5 mL vials or 6 well plates per Robotic Cooler</li><li>Maximum 1560 x 1.5 ml vials or 24 well plates per Liquid Handler</li><li>Short cycle times via fast and precise movements and the software option "overlapped injections" that allows for preparing the next injection while the previous is still running</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg" alt="Knauer: Liquid Handler LH 8.1 with AZURA system" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_AZURA_system1_7ad2b43571.jpg 244w," sizes="100vw" width="1390" height="889"><h3>Great variety of modules available</h3>KNAUER offers a range of active and passive modules for easy customisation of your LH 8.1.<ul><li>Want to have more samples and need a cooling option? Use the Robotic Cooler!</li><li>Want a lower carry over? Install the Fast Wash Station with two different solvents for in- and outside syringe and needle cleaning!</li><li>Want to change the solvent in your syringe? Use a passive solvent option to draw liquid during the injection process!</li></ul>There are a variety of active and passive options available. Just mount the module on the sampler, configure it in your software and you are ready to go.<h4>Injection Valve</h4>The injection valve consists of a valve drive and a 6 Port 2-Position valve with a special injection port.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg" alt="Knauer: Liquid Handler LH 8.1 - standalone" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_standalone1_5f21514cca.jpg 179w," sizes="100vw" width="1293" height="1127"><h4>Tray Holder</h4>The manual tray holder can store up to 130 standard HPLC vials and is recommended whenever no sample cooling is needed.<h4>Robotic Cooler</h4>The Robotic Cooler can store your samples at 4 - 40°C. You can insert up to 3 Sample Racks for 130 vials each, what means you can store up to 390 sample vials per Robotic Cooler.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg" alt="Knauer: Liquid Handler LH 8.1 - cooled tray" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_2_c6fd0672e9.jpg 239w," sizes="100vw" width="1595" height="1043"><h4>Fast Wash Station</h4>With the fast wash station, the injection syringe is cleaned on the in- and outside. It is always recommended, because it significantly reduces carry-over and offers a very user-friendly handling.&nbsp;<h4>Manual Wash Station</h4>Manual wash stations are an alternative to the fast wash station when carry-over is not the major concern. A manual wash station can be integrated in the manual tray holder with 5 x 10 ml vials or used as an additional module with 6 x 10 ml vials or 2 x 60 ml vials.<h4>Sample Trays</h4>Different sample trays can be used either in the manual rack holder or in the Robotic Cooler. Depending on your needs, they are made for analytical standard 1.5 ml vials or well plates.<h4>Syringes</h4>The most common syringes are implemented in the chromatography software packages. All optimized parameters are preconfigured to get quickly started.<h3>Special features</h3><h4>Injection modes</h4><h5>Sandwich loop: Zero sample loss with excellent reproducibility</h5>Tired of choosing between sample loss and high performance? The new injection mode offers both.<ul><li>KNAUER Liquid Handler LH 8.1 has a special injection mode. Next to standard injections like full loop and partial loop it has the opportunity to use the sandwich injection mode.</li><li>The sample is prepared inside a gastight syringe and then transferred into the HPLC System. In sandwich mode the sample is trapped between small gaps of air. This injection method only takes up the sample volume it needs and the air gaps prevent any peak broadening. Furthermore the pressure increase during the injection process will reduce the sample plug even more leading ot much narrower peak shapes and higher injection precision. &nbsp;</li><li>Ths injection method combines the zero sample loss of µl pickup and injection precision of partial loop injection.</li><li>Only the amount of sample that is injected is aspired by the syringe</li><li>Minimized sample widenening during injection process</li><li>Very high precision</li><li>No loss of sample</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg" alt="Knauer - Liquid Handler LH 8.1 - Sandwich Injection Mode" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Sandwich_Injection_Mode_Big_fb713e65a0.jpg 179w," sizes="100vw" width="2832" height="2475"><h5>Full loop</h5><ul><li>Sample loop is completely filled with the sample</li><li>Maximum reproducibility is achieved</li><li>Injection volume equals the loop volume</li><li>The sample loop will be overfilled to ensure a complete and reproducible filling</li></ul><h5>Partial loop</h5><ul><li>Sample loop is partially filled with programmed amount of sample&nbsp;</li><li>Injection mode is recommended for preparative tasks</li><li>Maximum injection volume equals 50 % of the loop volume</li><li>No sample loss</li></ul><h3>Large number of samples for high-throughput applications</h3>The Robotic Cooler offers space for up to 390 x 1.5 ml standard sample vials while maintaining a stable temperature.&nbsp;<ul><li>The Robotic Cooler compartment offers 6 places for sample racks. Two per drawer with a variety of options. One place can be used by a 60 x 1.5 ml vial rack, or 384 well plates or 96 well plates. Furthermore a double rack can be used to offer 130 x 1.5 mL vial positions, taking the total sample capacity to 390 x 1.5 ml vials.</li><li>Depending on the rail length a multitude of Robotic Coolers can be installed and used seperatly. Up to 4 Robotic Coolers on the longest rail.</li><li>The Robotic Cooler is software controlled, which enables a higher response speed and only open the doors so the injection syringe can reach the sample, to protect other samples from temperature fluctuations.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg" alt="Knauer: Liquid Handler LH 8.1 - high throughtput" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_cooled_tray_1_d2a6edf265.jpg 245w," sizes="100vw" width="1722" height="1040"><h3>Speed up your injection</h3>The LH 8.1 is optimized to save time during injection and movement.<ul><li>To safe time during the movement, the opening of the Robotic Cooler is software controlled and happens automatically.</li><li>To further speed up the injection, the sampler can apply the "overlapped injections" function, where the sample is already prepared inside the syringe.</li><li>Then the sample can be injected directly after the end of the previous run.&nbsp;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg" alt="Knauer: Liquid Handler LH 8.1 - Method_overlapped" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_2_e94a888086.jpg 245w," sizes="100vw" width="1121" height="310"><h3>Easy control via HPLC software&nbsp;</h3><ul><li>The LH 8.1 can be used just as comfortable as any HPLC Autosampler.</li><li>All parameters for recommended syringes, like injection speed, delay and sandwich build-up are already implemented and extensively tested in our Chromatography Software packages, so that you are ready to go!</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg" alt="Knauer: Liquid Handler LH 8.1 - SW control" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Liquid_Handler_LH_8_1_Method_overlapped_af91cd2ba5.jpg 188w," sizes="100vw" width="928" height="771">

KNAUER High Precision AZURA P 6.1L HPLC Pump

KNAUER AZURA P 6.1L HPLC pump with up to 1000 bar, precise gradient control, and low pulsation for reliable UHPLC workflows.

The KNAUER AZURA P 6.1L is a high-precision analytical HPLC pump designed for accurate and reproducible solvent delivery in both HPLC and UHPLC applications.The pump combines essential features of a modern pumping system, including low pulsation, high pressure capability, precise gradient formation, and flexible configuration. It is available in both HPG (high-pressure gradient) and LPG (low-pressure gradient) versions, with a flow range up to 50 mL/min.The system uses a dual serial piston mechanism with active pulsation compensation, ensuring excellent flow stability under all conditions. A low-dead-volume microfluidic mixer enables efficient and precise solvent mixing.Different pump heads are available:<ul><li>up to 10 mL/min at 862 bar</li><li>up to 5 mL/min at 1000 bar</li></ul>Optional configurations include biocompatible materials (PEEK, ceramic, titanium) and specialized setups for normal phase applications (e.g., hexane, heptane).<h4>Key Features</h4><ul><li>High-precision analytical HPLC/UHPLC pump</li><li>Pressure up to 1000 bar</li><li>Flow rates up to 50 mL/min</li><li>Dual piston system with low pulsation</li><li>Active pulsation compensation</li><li>HPG and LPG gradient configurations</li><li>Microfluidic mixer (50–600 µl)</li><li>RFID pump head detection</li><li>Optional degasser</li><li>Biocompatible configurations available</li><li>Robust operation with demanding solvents</li></ul><h3>Technical Specifications</h3><h4>Solvent Delivery</h4><ul><li>Pump type: analytical HPLC pump</li><li>Mechanism: dual serial piston</li><li>Flow range: 0.001 – 50 mL/min</li><li>Max pressure: up to 1000 bar</li><li>Flow accuracy: ± 0.25%</li><li>Precision: ≤ 0.04% RSD</li></ul><h4>Gradient</h4><ul><li>Range: 0–100% (0.1% steps)</li><li>HPG accuracy: ± 0.3%</li><li>HPG precision: &lt; 0.1% RSD</li><li>LPG accuracy: ± 0.3% to ± 2%</li><li>LPG precision: &lt; 0.5% RSD</li></ul><h4>Mixing</h4><ul><li>Microfluidic mixer</li><li>Volumes: 50 / 100 / 200 / 400 / 600 µl</li><li>Low dead volume</li></ul><h4>Degasser (optional)</h4><ul><li>2–4 channels</li><li>Max flow: 10 mL/min per channel</li><li>Residual O₂: &lt; 0.5 ppm</li><li>Membrane: fluoropolymer</li></ul><h4>Materials</h4><ul><li>PEEK, Tefzel®, Systec AF</li><li>Optional biocompatible materials (titanium, ceramic)</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN, analog inputs</li><li>Event outputs (TTL, relay)</li><li>Optional mobile control</li><li>RFID pump head detection</li></ul><h4>Operating Conditions</h4><ul><li>Temperature: 4–40 °C</li><li>Humidity: &lt; 90% non-condensing</li></ul><h4>Dimensions &amp; Power</h4><ul><li>Dimensions: 361 × 208 × 523 mm</li><li>Weight: 14.1 kg</li><li>Power supply: 100–240 V, 50–60 Hz</li><li>Power consumption: max 100 W</li></ul>

KNAUER High Precision AZURA P 8.1L UHPLC Pump

KNAUER AZURA P 8.1L UHPLC pump with up to 1240 bar, adaptive pulsation compensation, and high precision for demanding UHPLC applications.

The KNAUER AZURA P 8.1L is a high-performance UHPLC pump designed for ultra-fast and high-resolution chromatographic applications. With a maximum pressure of 1240 bar (124 MPa) and flow rates up to 5 mL/min, it meets the demands of modern UHPLC workflows.The pump features a dual serial piston mechanism combined with adaptive pulsation compensation, which operates independently of flow rate, pressure, and solvent type through real-time monitoring of eluent compressibility and variable piston stroke.Together with KNAUER’s advanced piston drive technology, this results in exceptional flow reproducibility under all operating conditions. High-precision in-house manufacturing ensures long piston seal lifetime and maximum system uptime.<h4>Key Features</h4><ul><li>UHPLC pump up to 1240 bar</li><li>Flow rates up to 5 mL/min</li><li>Dual serial piston system</li><li>Adaptive pulsation compensation</li><li>Excellent flow reproducibility</li><li>HPG gradient capability</li><li>Integrated 4-channel degasser</li><li>RFID pump head detection</li><li>Low baseline noise</li><li>High durability and long lifetime</li><li>Compatible with UHPLC and LC-MS systems</li></ul><h3>Technical Specifications</h3><h4>Solvent Delivery</h4><ul><li>Pump type: analytical UHPLC pump</li><li>Mechanism: dual serial piston</li><li>Max pressure: 1240 bar</li><li>Flow rate: up to 5 mL/min</li><li>Flow accuracy: ± 0.25%</li><li>Precision: ≤ 0.04% RSD</li></ul><h4>Gradient</h4><ul><li>Type: HPG (high-pressure gradient)</li><li>Range: 0–100% (0.1% steps)</li><li>Accuracy: ± 0.3%</li><li>Precision: &lt; 0.1% RSD</li></ul><h4>Degasser</h4><ul><li>Channels: 4</li><li>Max flow: 5 mL/min per channel</li><li>Residual O₂: &lt; 0.5 ppm</li><li>Technology: fluoropolymer membrane</li></ul><h4>Connections</h4><ul><li>Inlet: UNF 1/4-28 (1/8” tubing)</li><li>Outlet: UNF 10-32 (1/16” capillary)</li></ul><h4>Materials</h4><ul><li>PEEK, Tefzel®, Systec AF</li><li>Compatible with most solvents (except HCl and halogenated hydrocarbons)</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN, analog inputs</li><li>Event outputs (TTL, relay)</li><li>Optional mobile control</li><li>RFID pump head detection</li></ul><h4>Safety &amp; Operating Conditions</h4><ul><li>Leak sensor</li><li>Soft start and programmable pressure limits</li><li>Temperature: 4–40 °C</li><li>Humidity: &lt; 90% non-condensing</li></ul><h4>Dimensions &amp; Power</h4><ul><li>Dimensions: 361 × 208 × 523 mm</li><li>Weight: 26.7 kg</li><li>Power supply: 100–240 V, 50–60 Hz</li><li>Power consumption: max 310 W</li></ul>

KNAUER AZURA Autosampler AS 6.1L

KNAUER AZURA AS 6.1L autosampler with up to 768 samples, ultra-low carryover, and fast injection cycles for HPLC/UHPLC workflows.

The KNAUER AZURA® AS 6.1L is a high-performance autosampler designed for automated sample injection in HPLC/UHPLC systems, offering high capacity, precision, and flexibility for both routine and high-throughput applications.The autosampler supports:<ul><li>up to 768 sample positions (microtiter plates)</li><li>up to 108 standard 1.5 mL vials</li></ul>A highly effective internal and external needle wash system minimizes carryover to extremely low levels. The system is also fast, with a complete injection cycle taking less than one minute, including needle washing.Three injection modes are supported:<ul><li>full loop filling (highest precision)</li><li>partial loop filling (variable volumes)</li><li>µl pickup (loss-free injection of small volumes)</li></ul>The integrated ILD™ (Intermediate Loop Decompression) valve ensures accurate high-pressure injections without sample dilution and reduces pressure spikes, protecting the column.<h4>Key Features</h4><ul><li>Autosampler for HPLC/UHPLC systems</li><li>Up to 768 sample positions</li><li>Up to 108 vials (1.5 mL)</li><li>Ultra-low carryover (&lt; 0.0003%)</li><li>Fast injection cycle (&lt; 1 min)</li><li>3 injection modes (full loop, partial loop, µl pickup)</li><li>ILD™ technology for high-pressure injection</li><li>PASA™ (pressure-assisted sample aspiration)</li><li>Optional sample cooling (4–40 °C)</li><li>Missing vial/plate detection</li><li>Supports precolumn derivatization</li></ul><h3>Technical Specifications</h3><h4>Sample Injection</h4><ul><li>Injection volume range: 0.1 µl – 10 mL</li><li>Precision:<ul><li>Full loop: &lt; 0.3% RSD</li><li>Partial loop: &lt; 0.5% RSD</li><li>µl pickup: &lt; 1.0% RSD</li></ul></li><li>Accuracy: 0.2 µl (250 µl syringe)</li></ul><h4>Performance</h4><ul><li>Carryover:<ul><li>&lt; 0.0015% (standard)</li><li>&lt; 0.0003% (extended wash)</li></ul></li><li>Injections per vial: max. 9</li><li>Cycle time:<ul><li>7 s (same vial)</li><li>14 s (different vials)</li><li>&lt; 60 s total</li></ul></li></ul><h4>Capacity &amp; Compatibility</h4><ul><li>Microtiter plates: ANSI SLAS standard</li><li>Max vial/plate height: 47 mm</li></ul><h4>Additional Features</h4><ul><li>Headspace pressure (bubble prevention)</li><li>Precolumn derivatization</li><li>Sample tray cooling (optional, 4–40 °C)</li><li>Vial detection sensor</li></ul><h4>Materials</h4><ul><li>ETFE, PTFE, stainless steel, glass, Vespel</li><li>Wide chemical compatibility</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN / Ethernet</li><li>Analog inputs</li><li>TTL inputs (start, stop, freeze)</li><li>Relay output (alarm, marker)</li></ul><h4>Operating Conditions</h4><ul><li>Temperature: 10–40 °C</li><li>Humidity: 20–80%</li></ul>

KNAUER AZURA CT 2.1 Column Thermostat

KNAUER AZURA CT 2.1 column thermostat with Peltier control (5–85 °C), high stability, and optional eluent pre-heating for precise HPLC separations.

The KNAUER AZURA CT 2.1 is a cost-effective column thermostat designed for precise and stable temperature control in HPLC applications.The instrument uses a microprocessor-controlled Peltier element, enabling both heating and cooling in the range of 5–85 °C. Its high temperature stability allows for programming linear and non-linear temperature gradients, improving separation performance.For advanced applications, the system can be optionally equipped with an eluent pre-heating cartridge, ensuring consistent temperature conditions, improved selectivity, and better peak shapes.Its compact design supports flexible integration and accommodates multiple columns.<h4>Key Features</h4><ul><li>Column thermostat for HPLC applications</li><li>Temperature range 5–85 °C</li><li>Peltier technology (heating and cooling)</li><li>High temperature stability (±0.1 °C)</li><li>Accurate temperature control (±0.2 °C)</li><li>Programmable temperature gradients</li><li>Optional eluent pre-heating</li><li>Supports multiple columns</li><li>Compact and robust design</li><li>Built-in safety features</li></ul><h3>Technical Specifications</h3><h4>Thermostatting</h4><ul><li>Technology: Peltier (heating &amp; cooling)</li><li>Temperature range: 5–85 °C</li><li>Heating/cooling rate: 2 °C/min</li><li>Accuracy: ± 0.2 °C</li><li>Stability: ± 0.1 °C</li><li>Air circulation: fan-assisted bidirectional flow</li></ul><h4>Column Compartment</h4><ul><li>Max number of columns: up to 8</li><li>Max length: 325 mm</li><li>Max diameter: 35 mm</li><li>Compatibility:<ul><li>125 mm × 4.6 mm ID (with precolumn)</li><li>300 mm × 4.6 mm ID</li><li>300 mm × 16 mm ID</li></ul></li></ul><h4>Internal Dimensions</h4><ul><li>90 × 390 × 47 mm</li></ul><h4>Safety</h4><ul><li>Self-check and auto-calibration at startup</li><li>Adjustable shut-off temperature</li><li>Leak sensor with acoustic alarm</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN interface</li><li>Optional Mobile Control (stand-alone operation)</li></ul><h4>Power &amp; Dimensions</h4><ul><li>Power supply: 90–230 V, 50–60 Hz</li><li>Power consumption: 100 W</li><li>Dimensions: 150 × 470 × 310 mm</li><li>Weight: 8.4 kg</li></ul><h4>Optional Accessories</h4><ul><li>Eluent pre-heating cartridge<ul><li>for capillaries 0.1 mm / 0.18 mm ID</li></ul></li></ul>

Products from category HPLC components from KNAUER - page 2

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